BioSep: - sonosep
BioSep: - sonosep
BioSep: - sonosep
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The <strong>BioSep</strong> features<br />
• Cell filtration by ultrasonic<br />
resonance field<br />
• Continuous operation<br />
• Low shear environment<br />
• Simple design<br />
• Compact autoclavable<br />
device<br />
• Compact In-Situ-<br />
Sterilizable device<br />
• Scalable system<br />
The <strong>BioSep</strong> advantages<br />
• No physical filter surfaces<br />
to foul<br />
• No mechanical parts to fail<br />
(no moving parts)<br />
• Small retention volume<br />
• Rapid turn around<br />
(low hold-up times)<br />
• Increased efficiency<br />
• Wide flow range<br />
• High cell viability<br />
• Consistent culture<br />
environment<br />
• Easy installation<br />
• Easy automation<br />
• Minimal operator<br />
involvement<br />
• Clean-In-Place<br />
The main production systems which are used today for Mab production are stirred tanks.<br />
Homogeneous systems like a stirred tank represent the biggest unit reactor volume<br />
attainable today and with the highest unit production capacities. This capacity can be<br />
increased drastically with the use of a cell retention device.<br />
The <strong>BioSep</strong> will typically remove between 90 and 99% of the cells in the harvest stream at<br />
a reactor cell concentration of up to 20 million cells/ml. The separation efficiency of the<br />
<strong>BioSep</strong> system is defined as:<br />
( )<br />
SE = 100 % 1 – Ch<br />
Cb<br />
• SE is the separation efficiency<br />
• Ch is the cell concentration in the harvest<br />
• Cb is the cell concentration in the bioreactor<br />
The separation efficiency of the <strong>BioSep</strong> system (10L, 50L and 200L) is controlled<br />
by adjusting the acoustic power input to the resonator and the run/stop cycle time ratio<br />
for the <strong>BioSep</strong> and harvest pump.<br />
Pump control cable<br />
Compressed air<br />
Output<br />
cable<br />
Harvest<br />
Recirculation pump<br />
Feed<br />
Effects on the economics of an acoustic perfused process as compared<br />
to a batch process:<br />
• improved efficiency in medium use (e.g. lower serum concentrations in growth medium),<br />
up to 5 times more efficient. The trapped and returned cells use only nutrients to maintain<br />
their metabolism and for biosynthesis of products.<br />
• a factor 10-20 higher viable cell concentration<br />
• antibody production per reactor volume per day with a factor 10-100 fold higher than<br />
in a batch (high volumetric productivity).<br />
• better on-line control due to steady state condition<br />
• reduced exposure of the products to proteases<br />
• antibody concentration in harvest improved with a factor 2-5 fold compared to batch process<br />
• downstream processing of the secreted product is reduced by one step.
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