Phosphoproteomics-identified ERF110 affects ... - Plant Physiology

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C: Real-time RT-PCR analysis of ERF110 mRNA level in transgenic RNAi lines
erf110-1 and erf110-2.
Figure 3. Effect of ethylene on bolting time
A. Left panel, representative picture of ethylene response mutant plants at 25 days of
Arabidopsis growth on M/S medium without ACC treatment. Right panel, the
ethylene production precursor ACC delayed bolting time in the wild-type Arabidopsis.
None stands for M/S medium; 1 µM and 5 µM ACC are M/S media supplemented
with 1 µM and 5 µM ACC, respectively; AOA represents M/S medium supplemented
with 100 µM AOA; AOA plus 1 and 5 µM ACC stands for M/S medium
supplemented with 100 µM AOA and 1 and 5 µM ACC, respectively. *** represents p
< 0.001 when compared between treatments.
B: Bolting time of mutant plants that were grown on M/S medium with different
treatments. N, M/S medium (untreated); E, M/S medium supplemented with 5 µM
ACC (ACC-treated); O, M/S medium supplemented with 100 µM AOA
(AOA-treated); OE, M/S medium supplemented with both 100 µM AOA and 5 µM
ACC (AOA+ACC-treated). * represents p < 0.05 and *** represents p < 0.001 in
comparison with that of Col-0 by the Student’s t-test. Both ethylene-response mutant
ctr1-1 and ERF110 RNAi lines exhibited a delayed bolting phenotype after ACC
treatment.
Figure 4. Regulation of ERF110 gene expression in ACC-treated Arabidopsis and
mutants
A: Western blot analysis of endogenous ERF110 protein in wild-type, ctr1-1 and
erf110 transgenic lines. Two-week-old seedlings of Arabidopsis were subjected to
various AOA and ACC treatments. N, M/S medium (untreated); E, M/S medium
supplemented with 5 µM ACC (ACC-treated); O, M/S medium supplemented with
100 µM AOA (AOA-treated); OE, M/S medium supplemented with both 100 µM
AOA and 5 µM ACC (AOA+ACC-treated). Actin was used as the loading control.
B: Relative and integrated abundance of endogenous ERF110 protein among mutant
with 5 µM ACC (ACC-treated). ** represents p < 0.01 according to the Student’s
t-test compared with that of Col-0 under the same treatment.
C: Relative and integrated intensity of endogenous ERF110 protein among mutant
plants after AOA and AOA+ACC treatment. O, M/S medium supplemented with 100
µM AOA (AOA-treated); OE, M/S medium supplemented with both 100 µM AOA
and 5 µM ACC (AOA+ACC-treated). * represents p < 0.05, ** represents p < 0.01
and *** represents p < 0.001 according to the Student’s t-test compared with that of
Col-0 under the same treatments.