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Scientific Spring Meeting KNVM & NVMM 2012

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Athene A Into the deep: applications of next generation sequencing in clinical virology<br />

Chairs: M. Koopmans & R. Schuurman<br />

14:00 - 14:30 Discovery of Schmallenberg virus<br />

M. Beer (Germany)<br />

14:30 - 14:45 Longitudinal next generation “Deep” sequence analysis of Dual/Mixed HIV<br />

infected patients treated with Maraviroc demonstrates rapid selection for<br />

X4-predicted virus with extremely low FPR<br />

J. Symons<br />

14:45 - 15:00 Evaluation of persistence of resistant variants with ultra-deep pyrosequencing<br />

in chronic Hepatitis C patients treated with Telaprevir<br />

X.V. Thomas<br />

15:00 - 15:15 HIV-1 Reverse Transcriptase drug resistance mutations M184V, M184I and<br />

M184T have a differential impact on Entecavir incorporation and susceptibility<br />

M. Nijhuis<br />

15:15 - 15:30 Comparison between Sanger cloning sequencing and ultra-deep sequencing<br />

techniques for the characterization of Hepatitis C quasispecies<br />

C.K.Y. Ho<br />

Room 3 STI treatment failure: the need for early diagnosis!<br />

Chair: H.J. Thjie & H. de Vries<br />

14:00 - 14:30 Challenges to the public health control of bacterial STIs, in particular gonorrhoea<br />

C. Ison (United Kingdom)<br />

14:30 - 15:00 Questioning azitromycin for uncomplicated urogenital Chlamydia infection<br />

M. Vandenbruaene (Belgium)<br />

15:00 - 15:15 Recent trends in gonococcal resistance against third-generation cephalosporins<br />

and azithromycin<br />

A.P. van Dam<br />

15:15 - 15:30 Improved diagnostics of bacterial vaginosis with molecular techniques<br />

A.G.C.L. Speksnijder<br />

Room 4/5 Clinical microbiology<br />

Chairs: B.J.M. Vlaminckx & M.J.H.M. Wolfhagen<br />

14:00 - 14:15 Outcome of nosocomial Clostridium difficile infections; results of a multicenter<br />

cohort study<br />

M.P.M. Hensgens<br />

14:15 - 14:30 Prolonged bacterial culture to detect periprosthetic joint infection: how long is<br />

long enough?<br />

K. Waar<br />

14:30 - 14:45 Evaluation of several biochemical and molecular techniques for identification<br />

of Streptococcus pneumonia and Streptococcus pseudopneumoniae and detection<br />

of these bacteria in respiratory samples<br />

E. Wessels<br />

14:45 - 15:00 Routine identification of clinical isolates of anaerobic bacteria: matrix-assisted<br />

laser desorption/ionization time-of-flight mass spectrometry performs better<br />

than conventional identification methods<br />

M. Knoester<br />

15:00 - 15:15 Empiric therapy for sepsis needs adaptation due to increased resistance rates<br />

M.A. Leverstein - van Hall<br />

15:15 - 15:30 Antimicrobial susceptibility testing in ninety minutes by bacterial cell count<br />

monitoring<br />

N.L.A. Arents<br />

15

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