Scientific Spring Meeting KNVM & NVMM 2012

www.knvm.org
www.nvmm.nl
Scientific Spring Meeting
KNVM & NVMM
2012
17 & 18 April 2012
Papendal Hotel and Conference Centre, Arnhem
2 nd Announcement

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Program-at-a-glance
Tuesday April 17, 2012
EXHIBITION ROOM ATHENE B/C ROOM ATHENE A ROOM 3 ROOM 4/5 ROOM 6/7 ROOM 8/9
09:00 - 09:30 Registration
09:30 - 11:00 Plenary session
11:00 - 11:30 Coffee/tea
11:30 - 13:30
Plenary session
&
Award Ceremony
13:30 - 14:30 Lunch Lunch Symposium Diasorin KNVM Business Meeting
14:30 - 16:00
16:00 - 16:30 Coffee/tea
From STEC to HUSEC - EHEC
yesterday and today
West Nile virus emergence
in Europe
Surprising infectious diseases and
association with pathogens
16:30 - 18:00 Psittacosis Mycology Virology
18:00 - 18:30 Drinks
18:30 - 20:30 Dinner
20:30 - 22:15
"Poster session & Poster award
ceremony”
22:15 - 01:30 Party
Wednesday April 18, 2012
Multiresistent microorganisms in
hospitals: recognizing outbreaks,
management and responsibilities
Mycobacterial disease: new insights
to improve treatment and outcome
Role of oxygen in lifestyle choices
of microbial pathogens
Outer membrane proteins of Gram
negative bacteria and Mycobacteria,
similarities and differences
Photosynthetic microbes:
ecophysiology and applications
Physiology & system biology
EXHIBITION ROOM ATHENE B/C ROOM ATHENE A ROOM 3 ROOM 4/5 ROOM 6/7 ROOM 8/9
08:30 - 09:00 Registration
09:00 - 10:30
Multidrug resistance:
ESBL and carbapenemase
Vaccines are not forever Asplenia: infection and prevention
WMDI: Human genetic factors in
relation to microbial infection
Gene regulation via alternative
sigma factors and their role in
virulence
Differentiation in microbial multicellular
communities
10:30 - 11:00 Coffee/tea
11:00 - 12:30
Microbial education
&
social media
Kingdomcrossers, pathogens
hopping between plant,
animal and humans
12:30 - 14:00 Lunch
13:00 - 14:00 BBC-MMO Business Meeting
14:00 - 15:30 Microbial pathogenesis
15:30 - 16:00 Coffee/tea
16:00 - 17:30 NVMM Business Meeting
Into the deep: applications
of next generation sequencing
in clinical virology
Streptococci
STI treatment failure:
the need for early diagnosis!
Lyme arthritis and
neuroborreliosis: clinical
recognition and laboratory tests
Clinical microbiology
Type V secretion: mechanism and
use in vaccine development
CRISPR-Cas:
more than just phage defence
Robustness of complex microbial
communities: implications for
microbial pathology and food
fermentations
Biomarkers for microbial
robustness

Introduction
The spring meeting of the two Dutch Microbiological Societies (NVMM & KNVM) will be held on April
17 & 18 2012 in Papendal. Last year was a very special year, celebrating the 100th anniversary of the
Dutch Society of Microbiology, now the Royal Dutch society for Microbiology (KNVM). Last year we
had over a 1000 visitors at Papendal; let’s try and equal in 2012.
This year we have again a two-day program with on day one a plenary session. During the remaining
program many invited speakers from all over the world will join us at Papendal. The general theme of
this year is sustainability. Microbes make up most of the biodiversity on earth and microorganisms are
of critical importance for the cycling of nutrients, the degradation of various compounds and the global
climate. Knowledge of this helps us to develop strategies to utilize our natural resources in a long term
sustainable manner. Also within, patient care and treatment options sustainability is a theme that we
encounter more and more. Four renowned speakers will open the meeting in the plenary session on
Tuesday morning. Prof. Jef Huisman from the Institute for Biodiversity and Ecosystem Dynamics in
Amsterdam will talk on microbes in the environment, while Prof. Derek Lovely from the University of
Massachusetts will educate us on the electrifying area of electromicrobiology. Prof. Grant McFadden of
the University of Florida, USA, will explore how poxviruses can be recycled to treat cancer and Prof.
Fernando Baquero, MD, PhD, Ramón y Cajal University Hospital, will talk on sustainability of antibiotic
efficacy: from containment to restorative approaches.
Throughout the program you will find seminars and symposia addressing the issue of sustainability but
also the broad area of microbiologically from basic research to clinical applications is addressed in these
sessions. Since we had a record number of abstracts (2011 not included) we feel that more and more
this Spring meeting is an excellent way to get acquainted with all aspects of Microbiology in the
Netherlands and to meet all your colleagues in an pleasant and open atmosphere to discuss science,
exchange thoughts and open new avenues for collaborations or possibly find your next job.
As always, several prices will be awarded, both from NVMM and KNVM, so even You can be awarded.
Below you will find more information on the prizes and remember to nominate your PhD students or
Postdocs. To make the meeting even more interesting for young scientists, the Stichting Antonie van
Leeuwenhoek will again sponsor all presenting PhD students with grants.
We hope to see you all in Papendal and look forward to an exciting and inspiring meeting and share
new data and insight in Microbiology in all its aspects (NVMM).
The organizing committee
Jos A G van Strijp
Microbiology awards 2012:
Poster prize Three poster prizes will be awarded for the best poster presentation.
(1 st prize: Euro 200, 2 nd & 3 rd prize: Euro 100)
Kiem prize (Kiemprijs) Three Kiem prizes, Euro 500 each, will be awarded to three
PhD-students for a first scientific paper.
Best thesis award The best thesis within the broad area of microbiology will be awarded
with an amount of Euro 1000.
Postdoc award A prize of Euro 2000 is awarded to the postdoctoral fellow with
the best achievements in microbiology in the preceding years.
All prizes cover the areas of Microbiology in the broadest sense: parasitology, virology, bacteriology,
mycology, molecular studies, epidemiological studies, clinical microbiology, microbe-host interaction,
biotechnological aspects and further. Four committees will judge the proposals.
Award ceremony, Tuesday 17 April, 13:00 - 13:30 hours, Room Athene B/C
After the plenary session on Tuesday the winners of the Kiem prize, Best thesis award and Postdoc
award will be announced.
1

Organizing committee
Prof. dr. J.A.G. van Strijp, chair
Dr. A.J.W. van Alphen
Prof. dr. W. Bitter
Prof. dr. S. Brul
Prof. dr. L. Dijkhuizen
Dr. B. Duim
Dr. J.W.B. van der Giessen
Prof. dr. ir. M.S.M. Jetten
Prof. dr. M.P.G. Koopmans
Prof. dr. P. Rottier
Prof. dr. P.H.M. Savelkoul
Prof. dr. L.J. Stal
Dr. B.J.M. Vlaminckx
Dr. M.J.H.M. Wolfhagen
Prof. dr. H.A.B. Wösten
Prof. dr. ir. M.H. Zwietering
Poster committee
Prof. dr. ir. M.H. Zwietering, chair
Dr. W. van Schaik
Dr. A.M. Wensing
The Scientific Spring Meeting is organized by the Royal Dutch Society of Microbiology (KNVM) and the
Dutch Society of Medical Microbiology (NVMM).
Meeting secretariat:
P.O. Box 2428
5202 CK ’s-Hertogenbosch
Tel 073 - 700 35 00
Fax 073 - 700 35 05
info@congresscompany.com
www.congresscompany.com
Photo’s: KNVM
2

General information
Dates
Tuesday 17 & Wednesday 18 April 2012
Venue
Papendal Hotel and Conference Centre
Papendallaan 3
Arnhem
Tel 026 - 483 79 11
Website
Please check www.knvm.org or www.nvmm.nl or for registration and up-to-date program information
and for more information on the KNVM or NVMM.
Language
The language will be English during the scientific sessions.
Accreditation
The Scientific Spring Meeting 2012 is accredited by the NVMM with 6 points for Tuesday 17 April and 5
points for Wednesday 18 April.
Please note that in order to receive accreditation you need to supply the meeting secretariat with your
BIG-number.
Certificate of attendance
Certificates of attendance should be collected at the registration desk in the afternoon of the last day of
your attendance.
Registration
Please register yourself online via www.knvm.org or www.nvmm.nl.
Registration fee (incl. NTMM supplement, coffee, tea and lunch)
1 day 2 days
Members KNVM / NVMM Euro 110 Euro 195
Non-members Euro 200 Euro 295
Young Investigator’s Grant
The registration fee for presenting PhD-students and AIOS’ (oral or poster, presenting author only)
will be waved.
Grant “Antonie van Leeuwenhoek Stichting”
The “Antonie van Leeuwenhoek Stichting” supports PhD-students and AIOS’ to attend the spring
meeting and therefore they will sponsor the hotel costs (1 night, Hotel Van der Valk only, 2 persons per
room) for presenting PhD-students or AIOS’ (poster or oral, presenting author only). Please indicate,
when registering, whether you would need a hotel room and provide the name of your roommate.
Please note: Pre-registration is mandatory
3

Dinner & party
Dinner Monday 16 April Euro 40 per person
Dinner & party Tuesday 17 April Euro 40 per person
Please note: only a limited number of dinner tickets are still available for the dinner on Tuesday 17
April. Reservations will be accepted on first-come-first-serve basis.
Cancellation
Cancellations and refund requests must be submitted via e-mail to the meeting secretariat before 15
February 2012. Cancellations made before 15 February 2012 will be refunded less Euro 30 to cover
administration costs. All refunds will be made after the meeting.
Hotel accommodation
At this moment only a limited number of rooms are available in the Bilderberg Hotel. Please check
availability when registering online. Reservations will be accepted on first-come-first-serve basis.
Prices hotel accommodation (including breakfast and tax):
Single room Double room
Bilderberg (Wolfheze or De Buunderkamp) Euro 126,50 Euro 144
Note: the person who makes the reservation for a double room has to pay the full amount.
Payment
Payment is only possible by a “éénmalige machtiging” for participants with a Dutch bank- or bankgiro
account. Other participants will receive payment instructions in their confirmation.
On site you can pay cash, via credit card (Visa or MasterCard) or by PIN.
Name badges
Name badges will be send, via regular mail, to all participants 2-3 weeks before the start of the meeting.
All participants should wear their name badge throughout the meeting.
Registration desk
The registration desk will be open on Tuesday and Wednesday from 8:30 - 17:00 hours.
Exhibition, lunch break, coffee/tea break
Coffee/tea will be available at the exhibition during the breaks. The lunch will be served at the
exhibition during the lunch break. The exhibition is located in Room Sydney and in Foyer 1 & 2.
Hotel rooms
If you have reserved a hotel room in Papendal Hotel and Conference Centre you may collect the room
key as of 13:00 hours at the front desk of the hotel. Please make sure to check out before 10:00 hours.
4

Instructions for presenters
PowerPoint:
All meeting rooms are equipped with computers and data projectors. Please bring your presentation on
a USB-stick to the speaker service center by latest in the break before your presentation.
For special audiovisual requirements, please contact the meeting secretariat.
Poster Sessions:
All posters will be on display throughout the congress.
The poster session is scheduled on Tuesday evening, April 17 th . Even poster numbers are presented
from 20:30 - 21:15 hours and odd poster numbers from 21:15 - 22:00 hours.
The poster board numbers correspond with the abstract/poster numbers in the NTMM supplement you
will receive upon registration.
Please mount your poster on the corresponding poster board as of Tuesday April 17 th , 8:30 hours, but
no later than 18:30 hours.
The poster should be PORTRAIT style. Landscape poster can NOT be displayed.
The maximum poster size is: 90 x 120 cm (WIDTH x HEIGHT)
Directly after the poster session the poster prizes will be announced.
There are 3 poster prizes: the 1 st prize is Euro 200 and the 2 nd and 3 rd prize Euro 100 each.
Posters may be removed on Wednesday 18 April as of 9:00 hours. Posters that haven’t been removed at
Wednesday April 18 th , 17:00 hours will be removed by the organization.
Party Tuesday evening
The poster prize ceremony will be followed by a dance party open for all participants.
Papendal Hotel en Conference Centre
A route description can be found on www.congresscompany.com.
Papendal taxi
If you are travelling by train you may use a service of Papendal Hotel en Conference Centre called the
“Papendal taxi”. The Papendal taxi will bring you from Central Railway Station Arnhem to Papendal for
Euro 11,00 per person one-way. If you would like to make use of this service, please call taxi company
Linsen at 026 - 474 22 95 (mention the Papendal taxi). The taxi’s can be found at the “Sonsbeek side”
of the railway station. You have to pay at arrival at the hotel reception. The taxi driver will wait for a
ticket you have to give him. At the end of the meeting you can order a Papendal taxi at the hotel
reception to bring you to the railway station.
Bus
From Arnhem Central Station bus 105 (direction Harderwijk) will bring you directly to bus stop ‘West
End’. From that point, it is a 10 minute walk to Hotel and Conference Centre Papendal. From Monday to
Friday, between 7 am and 6 pm, it is also possible to take bus 109 (direction Sportcentrum Papendal).
This bus goes directly to Papendal. From the bus stop it is a 3
minute walk to the rear entrance of the hotel.
5

Scientific programme
MONDAY 16 APRIL 2012
13:00 - 17:15 National examination for medical microbiologists in training
Room 8/9
19:00 - 21:00 Dinner
Restaurant
TUESDAY APRIL 17 2012
09:00 - 09:30 Registration
09:30 - 11:00 PLENARY SESSION
Athene B/C Chair: W. Bitter
09:30 - 10:15 Complex dynamics in microbial communities
J. Huisman (The Netherlands)
10:15 - 11:00 Electromicrobiology
D.R. Lovley (USA)
11:00 - 11:30 Coffee/tea break
11:30 - 13:30 PLENARY SESSION
Athene B/C Chair: B.J.M. Vlaminckx
11:30 - 12:15 The curious road from poxvirus tropism to oncolytic virotherapy
G. McFadden (USA)
12:15 - 13:00 Sustainability of antibiotic efficacy: from containment to restorative approaches
F. Baquero (Spain)
13:00 - 13:30 Award ceremony
13:30 - 14:30 Lunch
13:30 - 14:30 Lunch Symposium Diasorin: The new Liaison® XL Murex Hepatitis and
Athene B/C Retrovirus Assays: experiences from the field
Chair: Dr. Ph.H. Rothbarth, Rijnland Ziekenhuis, Leiderdorp
Dr. M. Schutten, Erasmus MC, Rotterdam
Dr. C.F.M. Linssen, Atrium Medisch Centrum, Heerlen
13:30 - 14:30 KNVM Business meeting
Athene A
14:30 - 16:00 PARALLEL SESSIONS
Athene B/C From STEC to HUSEC - EHEC yesterday and today
Chair: R.A. Coutinho
14:30 - 15:00 EHEC O104:H4: Phylogenetic origin and pathogenesis
A. Mellmann (Germany)
15:00 - 15:30 Molecular epidemiology and clinical risk assessment of Shiga-producing E. coli (STEC)
A.W. Friedrich
6

15:30 - 15:45 Subtractive epidemiology of ESBL producing Enterobacteriaceae in the northern
Dutch-German border region
S. Surie
15:45 - 16:00 High acquisition rates of ESBL producing Enterobacteriaceae among Dutch travelers
S. Paltansing
Athene A West Nile virus emergence in Europe
Chairs: C. Reusken & M. Koopmans
14:30 - 15:00 Emergence of West Nile virus in Greece
A. Papa (Greece)
15:00 - 15:30 West Nile virus non-coding RNA: effects on pathogenicity and mosquito transmission
G.P. Pijlman
15:30 - 15:45 The role of immature virus particles in Dengue pathogenesis
J.M. da Silva Voorham
15:45 - 16:00 Detection of Dengue NS1 in travelers: a comparison of the performance of three
rapid diagnostic tests with the Platelia NS1 antigen ELISA
C. Liem
Room 3 Surprising infectious diseases and association with pathogens
Chair: E.J. Kuijper
14:30 - 14:45 An outbreak of histoplasmosis misdiagnosed as miliary tuberculosis among
participants of a tropical biology course in Uganda
M.A. Schouten
14:45 - 15:00 Outbreak of a multi-drug resistant Pseudomonas aeruginosa on the intensive
care unit of a tertiary care hospital in the Netherlands; a case-control study to
identify sources and risk factors
M. Knoester
15:00 - 15:15 The curious case of a man who slept in the daytime
M.B.B. McCall
15:15 - 15:30 Appendiceal spirochaetosis in children
L.J. Westerman
15:30 - 15:45 Asymptomatic carriage of Mycoplasma pneumoniae in the upper respiratory
tract of children
C. Vink
15:45 - 16:00 Pneumococcal pneumonia: the association between clinical severity and serotype
A.J.H. Cremers
Room 4/5 Multiresistent microorganisms in hospitals: recognizing outbreaks,
management and responsibilities
Chair: M. Vos
14:30 - 15:00 Recognizing outbreaks: pitfalls in diagnosing carbapenemase producing
microorganisms
T. van Ossewaarde
15:00 - 15:15 Management of outbreaks: guidelines and responsibilities - WIP guidelines on
multiresistant microorganisms
I.J.B. Spijkerman
15:15 - 15:30 Management of outbreaks: guidelines and responsibilities - Responsibilities in
an outbreak situation
G. Haringhuizen
15:30 - 15:45 Management of outbreaks: guidelines and responsibilities - SWAB guidelines
on the treatment of multiresistant microorganisms
J.W. Mouton
15:45 - 16:00 National surveillance of outbreaks
C.H.E. Boel
7

Room 6/7 Role of oxygen in lifestyle choices of microbial pathogens
Chairs: J. Stoof & A. van Vliet
14:30 - 15:00 Bacterial oxygen sensing: lessons taught by Escherichia coli
J. Green (United Kingdom)
15:00 - 15:30 General and oxidative stress responses in foodborne pathogens
T. Abee
15:30 - 15:45 Translational regulation of the respiratory electron transport chain of Neisseria
meningitidis by the Fur controlled small non-coding RNA NrrF
Y. Pannekoek
15:45 - 16:00 Genome-wide identification of Streptococcus pneumoniae genes essential for
growth and survival in CO2-poor environmental conditions
P.J. Burghout
Room 8/9 Photosynthetic microbes: ecophysiology and applications
Chairs: M. Al-Najjar & M. Kühl
14:30 - 15:00 Ecology of chlorophyll-d containing cyanobacteria
M. Kühl (Denmark)
15:00 - 15:30 Redirecting the intermediary metabolism of Synechocystis PCC6803 for
sustainable biofuel production
K.J. Hellingwerf
15:30 - 15:45 Energy budget and light utilization efficiency in photosynthetic microbial mats
M. Al-Najjar (Germany)
15:45 - 16:00 Transition between oxygenic and anoxygenic photosynthesis in cyanobacteria
from the Frasassi sulfidic springs
J.M.K. Klatt (Germany)
16:00 - 16:30 Coffee/tea break
16:30 - 18:00 PARALLEL SESSIONS
Athene B/C Psittacosis
Chairs: B. Mulder & W. Dorigo
16:30 - 17:00 Microbiology and pathogenesis of psittacosis
D. Vanrompay (Belgium)
17:00 - 17:15 Diagnostics and typing of psittacosis
E. Heddema
17:15 - 17:30 Veterinary aspects and control of psittacosis
O.F.J. Stenvers
17:30 - 17:45 Multi locus sequence typing of Chlamydia psittaci and Chlamydia pecorumfrom
birds and mammals reveals an association between Chlamydia genotypes and
host species
Y. Pannekoek
17:45 - 18:00 The challenges of serological prediction of chronic Q fever
L.M. Kampschreur
Athene A Mycology
Chairs: H.A.B. Wösten & P. Verweij
16:30 - 16:45 Discovery of a new azole resistance mechanism in Aspergillus fumigatus
through whole genome sequencing and sexual crossing
S.M.T. Camps
16:45 - 17:00 Mannitol-1-phosphate dehydrogenase is essential for the development of
extreme stress resistant fungal ascospores
T.T. Wyatt
8

17:00 - 17:15 Effects of corticosteroids on submerged growth of Aspergillus fumigatus and
Aspergillus niger
E. Bathoorn
17:15 - 17:30 Identification of moulds with Matrix Assisted Laser Desorption and Ionization-
Time of Flight Mass Spectrometry
A.L. Klink
17:30 - 17:45 Aspergillus fumigatus mycovirus infection is not dependent on the genetic
up-make of the host
J.M. Refos
17:45 - 18:00 Filamentous fungi in cystic fibrosis patients in the Netherlands
P.D. Terpstra
Room 3 Virology
Chair: H.G.M. Niesters
16:30 - 16:45 Active polyomavirus infection characterizes trichodysplasia spinulosa
M.C.W. Feltkamp
16:45 - 17:00 The paradox of maternal immunity as a risk factor for congenital
cytomegalovirus infection: a population-based prediction model
J.C. de Vries
17:00 - 17:15 Binding of avian coronavirus spike proteins to host factors reflects virus
tropism and pathogenicity
M.H. Verheije
17:15 - 17:30 The use of MMP-8 and MMP-9 to assess disease severity in children with viral
lower respiratory tract infections
G. Ferwerda
17:30 - 17:45 Norovirus in hospitalized children: clinical, epidemiological and virological features
J.C. Rahamat-Langendoen
17:45 - 18:00 Stimulation of TLR3 or TLR9 on dendritic cells and fibroblasts limits herpes
simplex virus type 1 infection in an IFNß-dependent and -independent way
R.J.L. Gaajetaan
Room 4/5 Mycobacterial disease: new insights to improve treatment and outcome
Chairs: J. van Ingen & B. Mulder
16:30 - 17:00 New insights question the current rifampicin dose in tuberculosis treatment
J.E.M. de Steenwinkel
17:00 - 17:30 Pharmacokinetics of nontuberculous mycobacterial disease treatment regimens
J. van Ingen
17:30 - 17:45 Minimum inhibition concentration of first and second line drugs against
Mycobacterium tuberculosis complex isolates in relation to genetic mutations
G. van der Laan
17:45 - 18:00 Energy metabolism, diarylquinolines and pyrazinamide: insight in the
molecular mechanism of drugs that may shorten tuberculosis treatment
D. Bald
Room 6/7 Outer membrane proteins of Gram negative bacteria and Mycobacteria,
similarities and differences
Chair: E.N.G. Houben
16:30 - 17:00 Outer membrane proteins in mycobacteria
M. Niederweis (USA)
17:00 - 17:30 Outer membrane proteins in Gram-negative bacteria: structure, function and
biogenesis
J.P.M. Tommassen
9

17:30 - 17:45 Differential detergent extraction of Mycobacterium marinum cell envelopes
reveals novel, extensively modified, outer membrane protein
A.D. van der Woude
17:45 - 18:00 Omp32 of Helicobacter pylori is involved in nickel and cobalt transport
J. Stoof (United Kingdom)
Room 8/9 Physiology & system biology
Chairs: S. Brul & M.H. Zwietering
16:30 - 16:45 Detection of genes essential for growth of respiratory pathogens
A. Zomer
16:45 - 17:00 Unraveling the small regulatory RNA network of Bacillus subtilis
R.A.T. Mars
17:00 - 17:15 From transcriptional landscapes to prediction of stress induced robustness
using biomarkers
H.M.W. den Besten
17:15 - 17:30 Characterisation of the biodiversity of spoilage Lactobacilli
J.W. Sanders
17:30 - 17:45 Protein complexes involved in the electron transport chain of anammox bacteria
N.M. de Almeida
17:45 - 18:00 A multi-platform flow device for microbial cultivation and microscopic analysis
B.M. Ryback
18:00 - 18:30 Drinks
Room Sydney
18:30 - 20:30 Dinner
Restaurant
Foyer 1 & 2
20:30 - 22:00 Poster session
22:00 - 22:15 Poster award ceremony
22:15 - 01:30 Party
Athene A
10

WEDNESDAY 18 APRIL 2012
08:30 - 09:00 Registration
09:00 - 10:30 PARALLEL SESSIONS
Athene B/C Multidrug resistance: ESBL and carbapenemase
Chair: J.W.T. Cohen Stuart
09:00 - 09:15 A case of New Delhi metallo-beta-lactamase 1 (NDM-1) in the Netherlands with
secondary transmission
T. Halaby
09:15 - 09:30 Prevalence of rectal carriage of extended-spectrum beta-lactamase producing
Enterobacteriaceae in hospitalised patients: 2010 and 2011
I. Willemsen
09:30 - 09:45 Optimizing the Dutch carbapenemase detection guideline for OXA-48 producing
E. coli associated with a large outbreak
J.W.T. Cohen Stuart
09:45 - 10:00 Surveillance of carbapenemase producing Enterobacteriaceae in The Netherlands
D.W. Notermans
10:00 - 10:15 The impact of clinical breakpoint changes on surveillance of antimicrobial
resistance in Enterobacteriaceae causing bacteraemia
A.K. van der Bij
10:15 - 10:30 Cefotaxime resistant Enterobacteriaceae in fecal samples of dogs and cats
A. Schoormans
Athene A Vaccines are not forever
Chairs: P.W.M. Hermans & F.R. Mooi
09:00 - 09:30 Towards predicting the antigenic evolution of influenza virus
R.A.M. Fouchier
09:30 - 09:45 Changes in the composition of the pneumococcal population in the Netherlands
after the implementation of the 7-valent pneumococcal vaccine
K.E.M. Elberse
09:45 - 10:00 Mucosal immunization protects mice against influenza virus-induced
pneumococcal otitis media
D.A. Diavatopoulos
10:00 - 10:15 Phylogeny of European Bordetella pertussis and the occurrence of vaccine
antigen deficient (VAD) mutants
A. Zeddeman
10:15 - 10:30 In search for novel pertussis vaccine targets: a comprehensive transcriptomic
and proteomic approach
D. Gouw
Room 3 Asplenia: infection and prevention
Chair: R.W. Vreede
09:00 - 09:30 Life-threatening infections due to asplenia
F.P. Kroon
09:30 - 10:00 A new LCI guideline for prevention of infections in patients with hypo- and
asplenia
A.J.J. Lammers
10:00 - 10:30 Efficacy of vaccinations after splenectomy
A. Meerveld-Eggink
11

Room 4/5 WMDI: Human genetic factors in relation to microbial infection
Chairs: E.C.J. Claas & J.W.A. Rossen
09:00 - 09:30 Human genetic susceptibility to bacterial infections
J.T. van Dissel
09:30 - 10:00 Genetic susceptibility to viral Lower Respiratory Tract Infections (LRTI) in Europe
A. Rautanen (United Kingdom)
10:00 - 10:30 Genetic predisposition to chronic mucocutaneous candidiasis
F. van de Veerdonk
Room 6/7 Gene regulation via alternative sigma factors and their role in virulence
Chair: W. Bitter
09:00 - 09:30 Role of alternative sigma factors of Mycobacterium tuberculosis in virulence
R. Manganelli (Italy)
09:30 - 10:00 Alternative sigma factor regulation in pseudomonas
M. Llamas (Spain)
10:00 - 10:15 Promoter propagation in prokaryotes
M.W.J. van Passel
10:15 - 10:30 A novel cell-surface signalling system uncovers the intimate relationships
between the components of this signalling cascade
K.C.J.T. Bastiaansen
Room 8/9 Differentiation in microbial multicellular communities
Chair: D. Claessen
09:00 - 09:30 Differentiation in multicellular Bacillus subtilis communities
D. López (Germany)
09:30 - 10:00 Heterogeneity in the fungal mycelium
H.A.B. Wösten
10:00 - 10:15 Common genes are required for architecturally complex colony formation and
sliding motility of Bacillus subtilis
A.T. Kovacs
10:15 - 10:30 Spatially resolving the secretome within the mycelium of the cell factory
Aspergillus niger
P. Krijgsheld
10:30 - 11:00 Coffee/tea break
11:00 - 12:30 PARALLEL SESSIONS
Athene B/C Microbial education & social media
Chair: A.J.W. van Alphen
11:00 - 11:30 Social media and microbial education
A.J. Cann (United Kingdom)
11:30 - 11:45 Distant learning
M. Reij
11:45 - 12:00 Social media and infection control: trending topic?
D. Beaujean
12:00 - 12:15 Post-doctoral e-learning for professionals in medical microbiology
J.W. Mouton
12:15 - 12:30 Blended learning in a laboratory school
E.M. van Hove
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Athene A Kingdomcrossers, pathogens hopping between plant, animal and humans
Chairs: J. van Doorn & P.H.M. Savelkoul
11:00 - 11:30 We only find what we look for: can enteric pathogens of animals live on and be
spread by plants?
I.K. Toth (United Kingdom)
11:30 - 12:00 Barriers and bypasses to lateral gene transfer in prokaryotes
T.A.L. Dagan (Germany)
12:00 - 12:15 Zoo animal gut microbiota as a reservoir of antibiotic resistance genes
T.D.J. Bello Gonzalez
12:15 - 12:30 Functional metagenomic analysis reveals selection for antibiotic resistance in
the gut microbiota during Intensive Care hospitalization
E.B. Buelow
Room 3 Streptococci
Chairs: S.H.M. Rooijakkers & H.J. Bootsma
11:00 - 11:15 Genetic identification and virulence contribution of the Group A streptococcal
antigen
N.M. van Sorge
11:15 - 11:30 A Streptococcus pneumoniae operon modifying interaction with host cells in a
capsule-dependent manner
J.J.E. Bijlsma
11:30 - 11:45 Pneumococcal immune evasion by Zinc metalloprotease C
G.J. Surewaard
11:45 - 12:00 Interaction between Streptococcus pneumoniae, receptors and brain endothelial
cells in an experimental meningitis model
F. Iovino
12:00 - 12:15 Identification of novel pneumococcal adherence factors by a combination of
genome-wide approaches
H.J. Bootsma
12:15 - 12:30 Membrane attack complex deposition on Gram-positive bacteria
E.T.M. Berends
Room 4/5 Lyme arthritis and neuroborreliosis: clinical recognition and laboratory tests
Chairs: A. van Dam & E.J. Kuijper
11:00 - 11:30 CXCL13 as a diagnostic marker for Lyme neuroborreliosis
T. Rupprecht (Germany)
11:30 - 11:45 Testing for Lyme Disease: when and how?
N. van Burgel
11:45 - 12:00 Lyme arthritis; clinical diagnosis or only laboratory confirmed?
A. Brandenburg
12:00 - 12:15 Comparison of the inter-laboratory performance of Lyme disease serology in
the Netherlands by quality assessment
T. Herremans
12:15 - 12:30 Performance of five VlsE containing immunoassays for the diagnosis of Lyme
borreliosis
A.P. van Dam
Room 6/7 Type V secretion: mechanism and use in vaccine development
Chair: S. Luirink
11:00 - 11:30 A generalised module for the selective extracellular accumulation of
recombinant proteins
I.R. Henderson (United Kingdom)
13

11:30 - 12:00 Structure and function of type Vc and type Ve autotransporters
D. Linke (Germany)
12:00 - 12:15 Versatile microbial Autotransporter platforms for the display and secretion of
multiple heterologous proteins
W.S.P. Jong
12:15 - 12:30 Specificity of target selection by the TpsB outer membrane transporters of
co-existing Two Partner Secretion systems of the human pathogen Neisseria
meningitidis
S. Rahman
Room 8/9 Robustness of complex microbial communities: implications for microbial
pathology and food fermentations
Chairs: D. Budding & E.J. Smid
11:00 - 11:30 Studying the gut microbiota using metagenomics
J. Raes
11:30 - 12:00 Multilevel approach reveals high level of clonal and functional diversity in
complex dairy starter culture
O. Erkus
12:00 - 12:15 Population dynamics in an undefined mixed starter culture - the role of
bacteriophages
M. Spus
12:15 - 12:30 Immune evasion dynamics during Staphylococcus aureus biofilm growth
R. Nijland
12:30 - 14:00 Lunch
13:00 - 14:00 BBC-MMO Business meeting
Athene A
14:00 - 15:30 PARALLEL SESSIONS
Athene B/C Microbial pathogenesis
Chairs: J.J.E. Bijlsma & N.M. van Sorge
14:00 - 14:15 Modulated lipooligosaccharide structure prevents Haemophilus influenzae from
IgM-mediated complement killing during otitis media
J.D. Langereis
14:15 - 14:30 Profiling the antibody response in acute and chronic patients from a recent
Dutch Q fever outbreak by protein microarray
T. Herremans
14:30 - 14:45 Secretome phage display library, filling the gap between genomic data and
functional studies
P.J.A. Haas
14:45 - 15:00 Induction of protective T-cell responses by Aspergillus fumigatus in humans
M.S. Gresnigt
15:00 - 15:15 A general secretion signal for the type VII protein secretion pathway in
pathogenic mycobacteria
M.H. Daleke
15:15 - 15:30 Staphylococcus aureus secretes three Extracellular adherence proteins (Eap)
that inhibit Neutrophil elastase
D.A.C. Stapels
14

Athene A Into the deep: applications of next generation sequencing in clinical virology
Chairs: M. Koopmans & R. Schuurman
14:00 - 14:30 Discovery of Schmallenberg virus
M. Beer (Germany)
14:30 - 14:45 Longitudinal next generation “Deep” sequence analysis of Dual/Mixed HIV
infected patients treated with Maraviroc demonstrates rapid selection for
X4-predicted virus with extremely low FPR
J. Symons
14:45 - 15:00 Evaluation of persistence of resistant variants with ultra-deep pyrosequencing
in chronic Hepatitis C patients treated with Telaprevir
X.V. Thomas
15:00 - 15:15 HIV-1 Reverse Transcriptase drug resistance mutations M184V, M184I and
M184T have a differential impact on Entecavir incorporation and susceptibility
M. Nijhuis
15:15 - 15:30 Comparison between Sanger cloning sequencing and ultra-deep sequencing
techniques for the characterization of Hepatitis C quasispecies
C.K.Y. Ho
Room 3 STI treatment failure: the need for early diagnosis!
Chair: H.J. Thjie & H. de Vries
14:00 - 14:30 Challenges to the public health control of bacterial STIs, in particular gonorrhoea
C. Ison (United Kingdom)
14:30 - 15:00 Questioning azitromycin for uncomplicated urogenital Chlamydia infection
M. Vandenbruaene (Belgium)
15:00 - 15:15 Recent trends in gonococcal resistance against third-generation cephalosporins
and azithromycin
A.P. van Dam
15:15 - 15:30 Improved diagnostics of bacterial vaginosis with molecular techniques
A.G.C.L. Speksnijder
Room 4/5 Clinical microbiology
Chairs: B.J.M. Vlaminckx & M.J.H.M. Wolfhagen
14:00 - 14:15 Outcome of nosocomial Clostridium difficile infections; results of a multicenter
cohort study
M.P.M. Hensgens
14:15 - 14:30 Prolonged bacterial culture to detect periprosthetic joint infection: how long is
long enough?
K. Waar
14:30 - 14:45 Evaluation of several biochemical and molecular techniques for identification
of Streptococcus pneumonia and Streptococcus pseudopneumoniae and detection
of these bacteria in respiratory samples
E. Wessels
14:45 - 15:00 Routine identification of clinical isolates of anaerobic bacteria: matrix-assisted
laser desorption/ionization time-of-flight mass spectrometry performs better
than conventional identification methods
M. Knoester
15:00 - 15:15 Empiric therapy for sepsis needs adaptation due to increased resistance rates
M.A. Leverstein - van Hall
15:15 - 15:30 Antimicrobial susceptibility testing in ninety minutes by bacterial cell count
monitoring
N.L.A. Arents
15

Room 6/7 CRISPR-Cas: more than just phage defence
Chairs: P. van Baarlen & R. Louwen
14:00 - 14:30 CRISPR: a small RNA guided immune system
S.J.J. Brouns
14:30 - 15:00 crRNA maturation: a key event in the activation of the CRISPR/Cas immune system
E. Charpentier (Sweden)
15:00 - 15:15 Transcription of Campylobacter jejuni CRISPR RNAs is initiated from a sigma70
promoter located in each separate CRISPR repeat
A.H.M. van Vliet (United Kingdom)
15:15 - 15:30 CRISPR-Cas system in the genomes of Campylobacter fetus subspecies
B. Duim
Room 8/9 Biomarkers for microbial robustness
Chairs: T. Abee & H. den Besten
14:00 - 14:30 Robustness of probiotics
P. Ross (Ireland)
14:30 - 15:00 Enhanced functionality LAB starters and probiotics
M. Kleerebezem
15:00 - 15:30 Bacterial spore stress resistance; a proteomics quest for bio-markers
S. Brul
15:30 - 16:00 Coffee/tea break
16:00 - 17:30 NVMM Business meeting
Athene B/C
16

Sponsors & Exhibitors
Abbott
Alere Health
Astellas Pharma NL
BaseClear
BD
Beldico Belgium
Bio Rad Laboratories
Bio Trading Benelux
Biognost
Biolegio
BioMerieux Benelux
Bodégro
Boom
Bruker Nederland
Cepheid Benelux
Check-Points
Clean Air Techniek
Clindia Benelux
DiaSorin
Elitech Benelux
Gen-Probe
Gilead Sciences
ITK Diagnostics
Kiestra Lab Automation
Luminex
Mediaproducts
Mediphos Medical Supplies
Menarini Diagnostics
Meridian Bioscience
Minigrip Nederland
MIPS
MLS
MSD
Oxoid
Qiagen Benelux
R-Biopharm
Roche Diagnostics
Sarstedt
Siemens Healthcare Diagnostics
Snijders Scientific
Tieto Netherlands Healthcare
Supported by:
Antonie van Leeuwenhoek Stichting
17

Floorplan exhibition - Room Sydney
Booth
number Company
1 Mediaproducts
2 Elitech Benelux
3 Boom
6 DiaSorin
7 Gilead Sciences
8 Biolegio
10 Kiestra Lab Automation
11 Astellas Pharma NL
12 BaseClear
14 MLS
15 Siemens Healthcare Diagnostics
16 MSD
17 MIPS
18 Biognost
19+20 BD
21+22 Abbott
23 Alere Health
25 Bio Rad Laboratories
18
Booth
number Company
26 Snijders Scientific
27 Gen-Probe
28 Check-Points
29 ITK Diagnostics
30 Bruker Nederland
31 Beldico Belgium
32 Luminex
33 Oxoid
34 Minigrip Nederland
35 Bodégro
36 Clean Air Techniek
37 Clindia Benelux
38 Mediphos Medical Supplies
39 R-Biopharm
40 BioMerieux Benelux
41 Sarstedt
42 Bio Trading Benelux
43 Roche Diagnostics

Floorplan exhibition - Foyer 1 & 2
Booth
number Company
44 Qiagen Benelux
49 Tieto Netherlands Healthcare
50 Cepheid Benelux
54 Menarini Diagnostics
55 Meridian Bioscience
19

Floorplan Papendal Hotel and Conference Centre
WWW WWW
WWW WWW
WWW WWW
Speaker
Service
Centre
20

www.knvm.org
www.nvmm.nl
Meeting secretariat:
P.O. Box 2428
5202 CK ’s-Hertogenbosch
Tel 073 - 700 35 00
Fax 073 - 700 35 05
info@congresscompany.com
www.congresscompany.com