Pricing information - Invitrogen
Pricing information - Invitrogen
Pricing information - Invitrogen
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
Custom DNA Primer Price List<br />
Order on-line: www.lifetechnologies.com/primers — simple, secure, convenient<br />
E-Mail orders: europrim@lifetech.com<br />
Quality guarantee<br />
We guarantee that each <strong>Invitrogen</strong> Custom Primer will be synthesised with the correct sequence as<br />
specified and meet the specifications stated on the Certificate of Analysis. If you are not completely<br />
satisfied, we will replace the product free of charge within 30 days of purchase, or issue a credit. Just<br />
contact technical support.<br />
Technical support<br />
We bring superior technical support in the form of our Euro Tech-Line for help in planning and troubleshooting<br />
a variety of primer applications such as mutagenesis, sequencing, PCR and gene construction.<br />
E-mail eurotech@lifetech.com for assistance.<br />
Save time by choosing the right Custom Primer for your application<br />
Synthesis scales, product yields and purification options can be very confusing – what you really want<br />
to know is “What do I need to order for my application?” We’ve simplified the process so that you can<br />
concentrate on your work, not the tools you need to do your work.<br />
Purification Method<br />
Desalt<br />
Recommended maximum primer length<br />
= 30 bases<br />
Cartridge<br />
Recommended maximum primer length<br />
= 60 bases<br />
HPLC<br />
Recommended maximum primer length<br />
= 55 bases<br />
PAGE<br />
Recommended maximum primer length<br />
= 100 bases<br />
4ST 000499<br />
Description<br />
Oligos are processed through normal<br />
phase chromatography column which<br />
removes salts but not failure sequences.<br />
Based on reverse phase chromatography;<br />
removes failure sequences from the<br />
completed synthesis.<br />
Reverse Phase High Performance Liquid<br />
Chromatography (HPLC) removes failure<br />
sequences or unincorporated label the<br />
same way as cartridge purification.<br />
Method used to differentiate full-length<br />
product from failure sequences based on<br />
size and conformation.<br />
Application Recommended purification<br />
Routine PCR, primers up to 30 bases long Desalt<br />
Specialised PCR, primers >30 bases long Cartridge<br />
Cloning, primers up to 55 bases HPLC<br />
Cloning, primers >55 bases long PAGE<br />
For research Use only. not intended For any animal or hUman therapeUtic or diagnostic Use.<br />
© 2012 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners.<br />
Benefit<br />
A salt-free DNA solution, ready-to-use;<br />
suitable for many PCR and sequencing<br />
applications without further purification.<br />
All <strong>Invitrogen</strong> Primers are provided<br />
desalted as standard<br />
Provides full-length sequences needed in<br />
some applications<br />
Guarantees highly purified primer required<br />
in some applications (≥85% full length).<br />
Provides the highest percentage of<br />
full-length oligos (≥85%) required for<br />
certain demanding applications such as<br />
mutagenesis or adapter production.
Ordering<br />
Order on-line: www.lifetechnologies.com/primers<br />
For orders placed by e-mail, please ensure that you include the following <strong>information</strong>:<br />
• Customer name and phone number • Invoice address • Delivery address<br />
• Sequence of oligonucleotide (5’ to 3’) • Purchase order number • Sequence name<br />
• Modifications • Scale of synthesis • Purity level<br />
Packaging format<br />
Primers are available in two formats: lyophilised in 2-mL microcentrifuge tubes or lyophilised in 96-well<br />
arrays for automated operations. A minimum of 48 primers must be ordered for the 96-well array format.<br />
Prices<br />
4ST 000499<br />
Base price Purification 5’ primary Amine 5’ phosphate<br />
Scale Plates Tubes Cartridge HPLC PAGE Plates Tubes Plates Tubes<br />
25 nmole €0,20 €0,29 N/A N/A N/A €6,43 €40,20 €6,43 N/A<br />
50 nmole €0,24 €0,40 €25,40 €50,25 N/A €8,57 €40,20 €8,57 €35,00<br />
200 nmole €0,49 €0,82 €25,40 €50,25 €63,25 €9,64 €40,20 €9,64 €35,00<br />
1 µmole N/A €4,59 €31,40 €81,50 €110,00 N/A €65,00 N/A €58,25<br />
10 µmole N/A €24,08 €224,00 €245,00 €265,00 N/A €275,00 N/A Inquire<br />
Scale Mixed bases Inosine (deoxy) Uracil (deoxy) Phosphorothioates (S-oligos)<br />
25 nmole No Charge €10,52 €10,52 N/A<br />
50 nmole No Charge €10,52 €10,52 €10,52<br />
200 nmole No Charge €10,52 €10,52 €12,94<br />
1 µmole No Charge €14,29 €17,14 €15,77<br />
10 µmole No Charge N/A N/A €40,60<br />
Scale 3’ Biotin 3’ phosphate 5’ Biotin 5’ HEX, TET or 6-FAM 5’ Fluorescein 5’ Alexa Fluor<br />
25 nmole N/A N/A €51,75 €93,75 €49,60 N/A<br />
50 nmole €89,00 €58,91 €51,75 €93,75 €49,60 €141,00<br />
200 nmole €89,00 €70,89 €51,75 €93,75 €49,60 €188,00<br />
1 µmole €109,14 N/A €102,00 €134,00 €90,00 N/A<br />
10 µmole N/A N/A €706,00 €996,00 €469,00 N/A<br />
*To determine Custom Primers price, use the following formula: (number of total bases) x (price/base) + (number of modified bases) x (additional price/base) + purification charge + 5’ or 3’modification charge.<br />
Plates Tubes<br />
Delivery Charge €56,50 €10,00<br />
For research Use only. not intended For any animal or hUman therapeUtic or diagnostic Use.<br />
© 2012 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners.
Delivery<br />
Next day delivery: Order 25 nmol desalted oligos online today before 10.00 GMT (Monday-Thursday) and<br />
receive them next business day for only €12 (up to 20 oligos, 7-30mer).<br />
The table below outlines typical delivery times. We will make every effort to ship your order within these times.<br />
Type of Oligonucleotides Ships within Delivery on<br />
Desalted unmodified ≤45 bases ordered before 12.00 GMT 1 business day 2 nd business day<br />
Desalted 46-100 bases: phosphorylated oligonucleotides, 2 business days 3 rd business day<br />
and cartridge purified oligonucleotides<br />
HPLC purity oligonucleotides: 1 µmole scale 4 business days 5th business day<br />
3’ & 5’ modified 5 business days 6th business day<br />
PAGE purity oligonucleotides: 1 µmole scale 6 business days 7th business day<br />
Phosphothianates: 10 µmole scale 15 business days 16 business days<br />
For a complete delivery schedule go to www.lifetech.com/primers<br />
All prices listed are valid in the Netherlands only and subject to changes without notice.<br />
For research Use only. not intended For any animal or hUman therapeUtic or diagnostic Use.<br />
© 2012 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners.<br />
4ST 000499
Change language