QIAamp UltraSens Virus Kit Handbook 12/00 (PDF

More documents

Recommendations

Info

QIAamp spin column at very low speeds (~250 x g) during the nucleic acid binding step. This ensures maximum nucleic acid recovery. Note: For best results, depending on your microcentrifuge model, the g-force required for centrifugation in step 5 of the protocol may need optimization (see “Important Notes Before Starting”, page 12). Sample types The QIAamp UltraSens protocol is designed for isolation of viral nucleic acids (e.g., those of HIV, HCV, or HBV) from 1 ml plasma or serum, or 1 ml from a pool of such cell-free body fluids. Such pools can consist of many samples from individual donors, and are usually more homogeneous than samples from single donors. Note: If you use the QIAamp UltraSens Virus Kit to purify nucleic acids from other types of samples, you may have to optimize the g-force used during centrifugation to ensure maximum yield. The optimal g-force depends on the type of sample: sample types with lower protein contents than plasma or serum may need more than the recommended 1200 x g to fully pellet the detergent– nucleic acid complexes. QIAamp UltraSens technology may not work well with plasma samples that contain unusually high amounts of lipids, and recovery of viral nucleic acids from such samples may be reduced. Reduced pellet size can be an indication that the reagent in Buffer AC is interacting with lipids in the sample. See the troubleshooting guide on page 20 for strategies to overcome this problem. 10 QIAamp UltraSens Virus Kit Handbook 12/2000
The QIAamp UltraSens Virus procedure QIAamp UltraSens Virus Kit Handbook 12/2000 11
Page 1 and 2: QIAamp ® UltraSens Virus Kit Handb
Page 3 and 4: Contents Kit Contents 4 Storage Con
Page 5 and 6: Storage Conditions QIAamp spin colu
Page 7 and 8: Technical Assistance At QIAGEN we p
Page 9: Introduction The QIAamp ® UltraSen
Page 13 and 14: membrane. Higher g-forces may lead
Page 15 and 16: Handling of QIAamp Spin Columns Owi
Page 17 and 18: of internal control to be added to
Page 19 and 20: contact with the bottom of the QIAa
Page 21 and 22: e) High amount of lipid in the samp
Page 23 and 24: Appendix Handling RNA Ribonucleases
Page 25 and 26: Ordering Information Product Conten
Page 27: QIAGEN Companies Please see the ins

QIAamp UltraSens Virus Kit Handbook 12/00 (PDF

Create successful ePaper yourself

Delete template?

Save as template?