QIAamp UltraSens Virus Kit Handbook 12/00 (PDF

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Alternatively, glassware can be treated with DEPC * (diethyl pyrocarbonate). Rinse the glassware with 0.1% DEPC (0.1% in water) overnight (12 hours) at 37°C, and then autoclave or heat to 100°C for 15 min to remove residual DEPC. Note: Corex ® tubes should be rendered RNase-free by treatment with DEPC and not by baking. This will reduce the failure rate of this type of tube during centrifugation. Electrophoresis tanks Electrophoresis tanks should be cleaned with detergent solution (e.g. 0.5% SDS), rinsed with water, dried with ethanol, and then filled with a solution of 3% H2O2. After 10 min at room temperature, the electrophoresis tanks should be rinsed thoroughly with RNase-free water. Solutions Solutions (water and other solutions) should be treated with 0.1% DEPC. DEPC will react with primary amines and cannot be used directly to treat Tris buffers. DEPC is highly unstable in the presence of Tris buffers and decomposes rapidly into ethanol and CO2. When preparing Tris buffers, treat water with DEPC first, and then dissolve Tris to make the appropriate buffer. DEPC is a strong, but not absolute, inhibitor of RNases. It is commonly used at a concentration of 0.1% to inactivate RNases on glass or plasticware or to create RNase-free solutions and water. DEPC inactivates RNases by covalent modification. Trace amounts of DEPC will modify purine residues in RNA by carboxymethylation. Carboxymethylated RNA is translated with very low efficiency in cell-free systems. However, its ability to form DNA:RNA or RNA:RNA hybrids is not seriously affected unless a large fraction of the purine residues have been modified. Residual DEPC must always be removed from solutions or vessels by autoclaving or heating to 100°C for 15 min. Add 0.1 ml DEPC to 100 ml of the solution to be treated and shake vigorously to bring the DEPC into solution or let the solution bake for 12 hours at 37°C. Autoclave for 15 min to remove any trace of DEPC. It may be desirable to test water sources for the presence of contaminating RNases since many sources of distilled water are free of RNase activity. Note: QIAamp UltraSens buffers are not rendered RNase-free by DEPC treatment and are therefore free of any DEPC contamination. * DEPC is a suspected carcinogen and should be handled with great care. Wear gloves and use a fume hood when using this chemical. 24 QIAamp UltraSens Virus Kit Handbook 12/2000
Ordering Information Product Contents Cat. No. QIAamp UltraSens Virus Kit (50) QIAamp UltraSens Virus Kit (250) For 50 nucleic acid preps: 50 QIAamp Spin Columns, Carrier RNA, Proteinase K, Collection Tubes (2 ml), RNase-free Buffers For 250 nucleic acid preps: 250 QIAamp Spin Columns, Carrier RNA, Proteinase K, Collection Tubes (2 ml), RNase-free Buffers 53704 53706 Related products QIAamp 96 Virus BioRobot Kit — for automated, high-throughput purification of viral nucleic acids from plasma, serum, and body fluids QIAamp 96 Virus BioRobot Kit (12) For 12 x 96 nucleic acid preps: 12 QIAamp 96 Plates, RNase-free Buffers, QIAGEN Protease, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), Carrier RNA, Caps 965642 QIAamp DNA Blood Kits — for genomic, mitochondrial, and viral DNA purification from blood and related body fluids QIAamp DNA Blood Mini Kit (50)* QIAamp DNA Blood Midi Kit (20)* QIAamp DNA Blood Maxi Kit (10)* * Larger kit sizes available; please inquire. For 50 DNA minipreps: 50 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml) For 20 DNA midipreps: 20 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml) For 10 DNA maxipreps: 10 QIAamp Maxi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (50 ml) 51104 51183 51192 QIAamp UltraSens Virus Kit Handbook 12/2000 25
Page 1 and 2: QIAamp ® UltraSens Virus Kit Handb
Page 3 and 4: Contents Kit Contents 4 Storage Con
Page 5 and 6: Storage Conditions QIAamp spin colu
Page 7 and 8: Technical Assistance At QIAGEN we p
Page 9 and 10: Introduction The QIAamp ® UltraSen
Page 11 and 12: The QIAamp UltraSens Virus procedur
Page 13 and 14: membrane. Higher g-forces may lead
Page 15 and 16: Handling of QIAamp Spin Columns Owi
Page 17 and 18: of internal control to be added to
Page 19 and 20: contact with the bottom of the QIAa
Page 21 and 22: e) High amount of lipid in the samp
Page 23: Appendix Handling RNA Ribonucleases
Page 27: QIAGEN Companies Please see the ins

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