Canine AI Introduction_en_121011 - MINITÜB GmbH
Canine AI Introduction_en_121011 - MINITÜB GmbH
Canine AI Introduction_en_121011 - MINITÜB GmbH
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5. Instrum<strong>en</strong>tal sem<strong>en</strong> transfer - Insemination technique<br />
The technique for artificial insemination dep<strong>en</strong>ds on the type of sem<strong>en</strong> to be used:<br />
Fresh sem<strong>en</strong><br />
Insemination using fresh sem<strong>en</strong> normally takes place immediately after the sem<strong>en</strong> is collected and the ejaculate is<br />
evaluated. Fresh sem<strong>en</strong> is g<strong>en</strong>erally introduced into the cranial part of the vagina.<br />
Chilled sem<strong>en</strong><br />
Chilled sem<strong>en</strong>, which is normally sem<strong>en</strong> that has be<strong>en</strong> ext<strong>en</strong>ded, chilled and ev<strong>en</strong>tually transported, can<br />
either be introduced into the vagina or it can be deposited into the uterus using an <strong>en</strong>doscope. Insemination<br />
using chilled sem<strong>en</strong> can take place several days after collection, due to advanced preservation media that are<br />
available today. After several days (dep<strong>en</strong>ds on the type of media used) sem<strong>en</strong> quality declines.<br />
Cryo preserved sem<strong>en</strong><br />
Cryo presevered sem<strong>en</strong> needs to be thawed just before insemination and must be deposited directly into the<br />
uterus, in order to achieve good insemination results. If the insemination of the bitch takes place with an<br />
<strong>en</strong>doscope, the dog remains conscious with the owner pres<strong>en</strong>t. Surgical transfer normally takes place under<br />
g<strong>en</strong>eral anaesthesia.
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