2012 Promega catalogue

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Life Science Catalog <strong>2012</strong> Worldwide Contact List Section Contents Table of Contents Cell Signaling 70 ProFluor ® Ser/Thr PPase Assay Product Size Cat.# Price ($) ProFluor ® Ser/Thr PPase Assay 4 plate V1260 1196.00 For Research Use Only. Not for Use in Diagnostic Procedures. 8 plate V1261 1967.00 Description: The ProFluor ® Ser/Thr PPase Assay measures purified serine/ threonine protein phosphatase activity in a multiwell plate format and involves “add-mix-read” steps only—ideal for high-throughput applications. The assay works with protein phosphatase 1 (PP1), PP2A, PP2B and PP2C. The assay begins with a standard phosphatase reaction performed with a provided phosphorylated bisamide rhodamine 110 peptide substrate (S/T PPase R110 Substrate) and Control AMC Substrate that serves as a control for compounds that may inhibit the protease reaction. Following the phosphatase reaction, a termination buffer containing a protease reagent is added, which both stops the phosphatase reaction and removes amino acids specifically from the nonphosphorylated substrate, liberating highly fluorescent rhodamine 110. However, phosphorylated substrate, however, is resistant to digestion by the protease reagent and remains nonfluorescent. Thus, fluorescence intensity measured in this assay is directly correlated with phosphatase activity. The assay produces excellent Z´ values (>0.8) in either 96- or 384-well plate formats and easily distinguishes known phosphatase inhibitors from other compounds. Features: • Achieve Highly Predictive Results: Robust Z´ values greater than 0.7 in either 96- or 384-well plate formats. • Minimal Test Compound Interference: Fluorescent signal from Rhodamine 110 is much greater than signals from by test compounds. • Control Peptide Included: Use AAF-AMC control peptide to monitor protease activity and reduce false-positive hits. • Simplify Your Assays: Add-mix-read format reduces the steps. • Non-Radioactive: No radioactive waste disposal and safety issues. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store the entire system at –20°C. Protect the S/T PPase R110 Substrate and Control AMC Substrate from light. Protocol Part# ProFluor ® Ser/Thr PPase Assay Technical Bulletin TB324 RFU P P No Phosphatase P P Protease Reagent P P Nonfluorescent 160,000 140,000 120,000 100,000 80,000 60,000 40,000 20,000 r 2 = 0.99 P P S/T Phosphatase Protease Reagent Fluorescent 0 0 10 20 30 40 50 60 70 80 90100 Percent Nonphosphorylated Peptide Effect of phosphopeptide content on fluorescence intensity. The graph shows the average RFU obtained after a 90-minute digestion of mixtures of nonphosphorylated S/T PPase R110 Substrate and phosphorylated substrate as indicated to mimic a phosphatase titration. 4166MB ProFluor ® Tyrosine Phosphatase Assay Product Size Cat.# Price ($) ProFluor ® Tyrosine Phosphatase Assay 4 plate V1280 1185.00 For Research Use Only. Not for Use in Diagnostic Procedures. 8 plate V1281 2040.00 Description: The ProFluor ® Tyrosine Phosphatase Assay measures purified tyrosine phosphatase enzyme activity in a multiwell plate format and involves “add-mix-read” steps only—ideal for high-throughput applications. Tyrosine phosphatases tested with the assay include PTP-1B, CD45, LAR PTPase and YOP-51. The assay begins with a standard phosphatase reaction performed with a provided phosphorylated bisamide rhodamine 110 peptide substrate (PTPase R110 Substrate) and Control AMC Substrate that serves as a control for compounds that may inhibit the protease. Following the phosphatase reaction, a termination buffer containing a protease reagent is added, which simultaneously stops the phosphatase reaction and removes amino acids specifically from the nonphosphorylated substrate, liberating highly fluorescent rhodamine 110. Phosphorylated substrate, however, is resistant to digestion by the protease reagent and remains nonfluorescent. Thus, fluorescence intensity measured in this assay is directly correlated with phosphatase activity. The assay produces excellent Z´ values (>0.7) in either 96- or 384-well plate formats and easily distinguishes known phosphatase inhibitors from other compounds. Features: • Achieve Highly Predictive Results: Robust Z´ values greater than 0.8 in either 96- or 384-well plate formats. • Observe Minimal Test Compound Interference: Substrate used at micromolar concentration. Rhodamine 110 fluorescent signal produced is much greater than the fluorescent signal given off by test compounds. • Control Peptide Included: Control peptide (AAF-AMC) included that is used to monitor protease activity. Reduces false positive hits. • Simplify Your Assays: Simple add-mix-read format reduces the number of plate-handling steps to fewer than that required for other phosphatase assays. • Save Time: Minimal throughput time compared to the multiple steps and lengthy incubations with other phoshatase assays. • Non-Radioactive: No radioactive waste disposal and safety issues. • Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ Storage Conditions: Store the entire system at –20°C. Protect the PTPase R110 Substrate and Control AMC Substrate from light. Protocol Part# ProFluor ® Tyrosine Phosphatase Assay Technical Bulletin TB334 For complete and up-to-date product information visit: www.promega.com/catalog
Cell Signaling For complete and up-to-date product information visit: www.promega.com/catalog 4 Cloning and DNA Markers Molecular Weight Markers 72 Restriction Enzymes 79 Alkaline Phosphatases 88 Polymerases 89 Ligases 92 Kinases (PNK) 93 Nucleases 93 Additional Enzymes 95 Ribonuclease Inhibitors 96 Subcloning Tools and Vectors 97 Bacterial Strains and Competent Cells 104 71 4 Cloning and DNA Markers Section Contents Table of Contents
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Life Science CATALOG 2012
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Cell Signaling Table of Contents Pr
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Cell Signaling For complete and up-
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Cell Signaling Agarose, LMP, Prepar
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Cell Signaling Blue/Orange Loading
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Cell Signaling Glycine, Molecular B
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Cell Signaling Sephacryl ® S-400 P
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Cell Signaling Tris Base, Molecular
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Cell Signaling Unmethylated Lambda
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Cell Signaling Promega Barrier Tips
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Cell Signaling Product Size Cat.# P
Page 23 and 24: Cell Signaling 2 Cell Health Assays
Page 25 and 26: Cell Signaling A. B. Luminescence/p
Page 27 and 28: Cell Signaling Luminogenic Enzyme S
Page 29 and 30: Cell Signaling Apoptosis
Page 31 and 32: Cell Signaling Caspase-Glo ® 2 Ass
Page 33 and 34: Cell Signaling Caspase-Glo ® 9 Ass
Page 35 and 36: Cell Signaling CaspACE Assay System
Page 37 and 38: Cell Signaling Anti-ACTIVE ® Caspa
Page 39 and 40: Cell Signaling BacTiter-Glo Microbi
Page 41 and 42: Cell Signaling CellTiter 96 ® Cell
Page 43 and 44: Cell Signaling MultiTox-Fluor Multi
Page 45 and 46: Cell Signaling CytoTox-Glo Cytotoxi
Page 47 and 48: Cell Signaling CytoTox 96 ® Non-Ra
Page 49 and 50: Cell Signaling GSH-Glo Glutathione
Page 51 and 52: Cell Signaling 3 Cell Signaling For
Page 53 and 54: Cell Signaling cAMP-Glo Max Assay P
Page 55 and 56: Cell Signaling Growth Factors Epide
Page 57 and 58: Cell Signaling SIRT-Glo Assays and
Page 59 and 60: Cell Signaling ADP-Glo Max Assay Pr
Page 61 and 62: Cell Signaling Kinase Enzyme System
Page 63 and 64: Cell Signaling Kinase Enzyme System
Page 65 and 66: Cell Signaling Kinase-Glo ® Kinase
Page 67 and 68: Cell Signaling SignaTECT ® Protein
Page 69 and 70: Cell Signaling Casein Kinase I Prod
Page 71 and 72: Cell Signaling Product Size Cat.# P
Page 73: Cell Signaling Protein Kinase Subst
Page 77 and 78: Cell Signaling DNA Step Ladders Pro
Page 79 and 80: Cell Signaling Conventional DNA Mar
Page 81 and 82: Cell Signaling ProMega-Markers ® L
Page 83 and 84: Cell Signaling Restriction Enzymes
Page 85 and 86: Cell Signaling BamHI Product Size C
Page 87 and 88: Cell Signaling EcoRI Product Size C
Page 89 and 90: Cell Signaling NciI Product Size Co
Page 91 and 92: Cell Signaling SpeI Product Size Co
Page 93 and 94: Cell Signaling TSAP Thermosensitive
Page 95 and 96: Cell Signaling T3 RNA Polymerase Pr
Page 97 and 98: Cell Signaling Kinases T4 Polynucle
Page 99 and 100: Cell Signaling S1 Nuclease Product
Page 101 and 102: Cell Signaling Percent Inhibition o
Page 103 and 104: Cell Signaling Untagged Flexi ® Ma
Page 105 and 106: Cell Signaling pGEM ® -5Zf(+) Vect
Page 107 and 108: Cell Signaling pSP64 Poly(A) Vector
Page 109 and 110: Cell Signaling For complete and up-
Page 111 and 112: Cell Signaling x-tracta Gel Extract
Page 113 and 114: Cell Signaling Wizard ® MagneSil
Page 115 and 116: Cell Signaling ReliaPrep FFPE gDNA
Page 117 and 118: Cell Signaling MagneSil ® ONE, Fix
Page 119 and 120: Cell Signaling MagneSil ® KF, Geno
Page 121 and 122: Cell Signaling Plasmid Purification
Page 123 and 124: Cell Signaling PureYield Plasmid Ma
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Cell Signaling Wizard ® Plus Maxip
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Cell Signaling Wizard ® MagneSil
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Cell Signaling PureYield RNA Midipr
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Cell Signaling Maxwell ® 16 System
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Cell Signaling RNasin ® Plus RNase
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Cell Signaling DNA Quantitation Qua
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Cell Signaling Magnetic Stands and
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Cell Signaling 6 Drug Discovery For
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Cell Signaling GPCR Assays cAMP-Glo
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Cell Signaling PDE-Glo Phosphodiest
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Cell Signaling Protease Assays Prot
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Cell Signaling Cell-Based Proteasom
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Cell Signaling Tryptase, Human, Rec
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Cell Signaling 7 Genetic Identity F
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Cell Signaling Maxwell ® 16 Forens
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Cell Signaling Plexor ® HY System
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Cell Signaling PowerPlex ® 18D Sys
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Cell Signaling PowerPlex ® CS7 Sys
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Cell Signaling PowerPlex ® 16 and
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Cell Signaling GenePrint ® Fluores
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Block & Sample 5X Bu
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Cell Signaling Anti-ACTIVE ® JNK p
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Cell Signaling Anti-ERK 1/2 pAb, Ra
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Cell Signaling Anti-Human GDNF pAb
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Cell Signaling Anti-PARP p85 Fragme
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Cell Signaling Anti-VAChT pAb Produ
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling Microbial Detection
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10 Instruments For complete and up-
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GloMax ® 20/20 Luminometer Product
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Fluorescence Module: Application-op
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GloMax ® -Multi Jr Single-Tube Mul
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Maxwell ® 16 Instruments Maxwell
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Maxwell ® 16 Flexi Method Firmware
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Cell Signaling Maxwell ® 16 System
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Cell Signaling Maxwell ® 16 Flexi
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Cell Signaling dNTP Mix Product Siz
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Cell Signaling 12 PCR For complete
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Cell Signaling TaqBead Hot Start Po
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Cell Signaling GoTaq ® PCR Core Sy
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Cell Signaling Deoxynucleotide Trip
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Cell Signaling Plexor ® qPCR and q
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Cell Signaling StemElite Differenti
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Cell Signaling Reverse Transcriptio
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Cell Signaling AMV Reverse Transcri
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Cell Signaling PCR Cloning pGEM ®
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Cell Signaling TnT ® Quick Coupled
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Cell Signaling TnT ® T7 Quick for
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Cell Signaling Canine Pancreatic Mi
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Cell Signaling pGEM ® β-Gal Contr
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Cell Signaling Immobilized Trypsin
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Cell Signaling Asp-N, Sequencing Gr
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Inducible T7-Driven
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Cell Signaling ProtoBlot ® II AP S
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Cell Signaling HaloTag ® Protein P
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Cell Signaling HaloTag ® Mammalian
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Cell Signaling HaloLink Protein Arr
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Cell Signaling HaloCHIP System Prod
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Cell Signaling MagneHis Protein Pur
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Cell Signaling Streptavidin Product
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Cell Signaling Protein Purification
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Cell Signaling 15 Reporter Assays a
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Cell Signaling Dual-Glo ® Lucifera
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Cell Signaling Firefly Luciferase R
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Cell Signaling Bright-Glo Luciferas
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Cell Signaling EnduRen Live Cell Su
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Cell Signaling Beta-Glo ® Assay Sy
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Cell Signaling Promoter-Driven Cont
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Cell Signaling Nuclear Receptor Pat
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Cell Signaling pRL Renilla Lucifera
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Cell Signaling GloResponse Lucifera
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Cell Signaling pSP-luc+NF Fusion Ve
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling Transfection Reagent
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Cell Signaling 16 RNA Analysis For
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Cell Signaling Riboprobe ® System
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Cell Signaling HeLaScribe ® Nuclea
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Cell Signaling RNA Interference Gen
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Cell Signaling 17 Stem Cell Researc
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Cell Signaling Cell ID System Produ
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Cell Signaling StemElite Human Panc
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Cell Signaling 18 Vectors For compl
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Cell Signaling HaloTag ® Fusion (N
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Cell Signaling pCI Mammalian Expres
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Cell Signaling Reporter Vectors pGL
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Cell Signaling pGEM ® -luc DNA Pro
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Cell Signaling pGEM ® and pSP Subc
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Cell Signaling pUC/M13 Sequencing P
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Index: A-Z 19 Index For complete an
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BssHII.............................
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In vitro Transcription Systems Rela
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QuantiFluor dsDNA System ..........
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Cat# Product Size Page Price($) A10
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Index by Catalog Number Cat# Produc
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Cat# Product Size Page Price($) C84
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Cat# Product Size Page Price($) E13
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Cat# Product Size Page Price($) E61
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Cat# Product Size Page Price($) G13
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Cat# Product Size Page Price($) G77
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Cat# Product Size Page Price($) L50
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Cat# Product Size Page Price($) P16
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Cat# Product Size Page Price($) R99
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Cat# Product Size Page Price($) V42
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Cat# Product Size Page Price($) W39
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personal DETECTION GloMax Integrat
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