SANCO/11368/2011-EN Rev. 3 - Izba Mleka

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Lead Not more than 2 mg/kg
Mercury Not more than 1 mg/kg
Cadmium Not more than 1 mg/kg
E 101 (ii) RIBOFLAVIN-5′-PHOSPHATE
Synonyms Riboflavin-5′-phosphate sodium
Definition These specifications apply to riboflavin 5′-phosphate
together with minor amounts of free riboflavin and
riboflavin diphosphate.
Colour Index No
EINECS 204-988-6
Chemical name Monosodium(2R,3R,4S)-5-(3′)10′-dihydro-7′,8′dimethyl-2′,4′-dioxo-10′-benzo[γ]pteridinyl)-2,3,4trihydroxypentyl
phosphate; monosodium salt of 5′monophosphate
ester of riboflavin
Chemical formula For the dihydrate form: C17H20N4NaO9P · 2H2O
Molecular weight 514,36
For the anhydrous form: C17H20N4NaO9P
Assay Content not less than 95 % total colouring matters
calculated as C17H20N4NaO9P.2H2O
E1 cm 1 % 250 at ca 375 nm in aqueous solution
Description Yellow to orange crystalline hygroscopic powder, with
slight odour
Identification
Purity
Spectrometry The ratio A375/A267 is
between 0,30 and 0,34
The ratio A444/A267 is
between 0,35 and 0,40
Maximum in water at ca 375 nm
in aqueous solution
Specific rotation [α]D 20 between + 38° and + 42° in a 5 molar HCl
solution
Loss on drying Not more than 8 % (100 °C, 5 hours in vacuum over
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