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chromID ™ Salmonella Brochure - bioMerieux

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inspired By Nature<br />

<strong>Salmonella</strong> Enteritidis<br />

<strong>chromID</strong> <strong>Salmonella</strong><br />

Highlight in colour!<br />

Chromogenic medium for the detection of <strong>Salmonella</strong> in food products


07-07 / 006GB99059C / This document is not legally binding. bioMérieux reserves the right to modify specifications without notice<br />

BIOMÉRIEUX, the blue logo, API, SM and VIDAS are registered and protected trademarks belonging to bioMérieux sa or one of its subsidiaries<br />

Chromogenic medium for the selective isolation and differentiation of the genus <strong>Salmonella</strong> in food products.<br />

A new principle for easier reading<br />

• Specificity : 3 chromogenic substrates optimize the<br />

selective isolation and differentiation of <strong>Salmonella</strong>,<br />

including the serotypes Typhi, Paratyphi and most Lactose<br />

positive <strong>Salmonella</strong><br />

Colour<br />

Esterase ß-glucosidase +<br />

of colonies<br />

ß-galactosidase<br />

Pale-pink<br />

to mauve<br />

Presumptive<br />

<strong>Salmonella</strong><br />

+<br />

-<br />

=> Perform complete identification (biochemical and/or immunological tests)<br />

Pale blue<br />

to colourless<br />

Other bacteria<br />

and yeasts<br />

• Nutrient capacity : the rigorous selection of ingredients<br />

provides large <strong>Salmonella</strong> colonies with irregular edges and<br />

an intense colouration, after only 18 to 24 hours incubation at<br />

37°C<br />

• Selectivity : the selective system chosen ensures excellent<br />

inhibition of contaminating food flora: Gram-positive cocci,<br />

yeasts and the majority of non-fermenting Gram-negative<br />

organisms<br />

<strong>bioMerieux</strong> offers a complete range of products<br />

for the isolation of <strong>Salmonella</strong><br />

<strong>chromID</strong> <strong>Salmonella</strong><br />

20 plates . . . . . . . . . . . . Ref. 43621<br />

100 plates . . . . . . . . . . . Ref. 43629<br />

XLD Agar<br />

20 plates . . . . . . . . . . . . Ref. 43563<br />

100 plates . . . . . . . . . . . Ref. 43564<br />

Brilliant Green Agar modified<br />

20 plates . . . . . . . . . . . . . Ref. 43588<br />

Hektoen Agar<br />

20 boîtes . . . . . . . . . . . Ref. 43111<br />

XLT4 Agar<br />

20 plates . . . . . . . . . . . . Ref. 43701<br />

API ® 20 E<br />

25 tests . . . . . . . . . . . . . Ref. 20100<br />

ID 32 E<br />

25 tests . . . . . . . . . . . . . Ref. 32400<br />

VIDAS ® Immuno-Concentration<br />

<strong>Salmonella</strong><br />

60 tests . . . . . . . . . . . . . Ref. 30435<br />

VIDAS ® <strong>Salmonella</strong><br />

60 tests . . . . . . . . . . . . . Ref. 30702<br />

-/+<br />

-<br />

<strong>Salmonella</strong> remains one of the main<br />

causes of food infections<br />

in industrialized countries<br />

bioMérieux S.A.<br />

69280 Marcy l’Etoile<br />

France<br />

Tel. : 33 (0)4 78 87 20 00<br />

Fax : 33 (0)4 78 87 20 90<br />

www.biomerieux.com<br />

M broth (VIDAS)<br />

20 x 10 ml . . . . . . . . . . Ref. 42077<br />

ICS broth (VIDAS)<br />

100 x 2 ml . . . . . . . . . . Ref. 42610<br />

Buffered peptone water<br />

6 x 225 ml . . . . . . . . . . Ref. 42043<br />

100 x 9 ml . . . . . . . . . . Ref. 42111<br />

6 x 90 ml . . . . . . . . . . . Ref. 42042<br />

3 x 3 l (BAG) . . . . . . . . . Ref. 42629<br />

10 x 225 ml (MINIBAG) . . Ref. 42729*<br />

Rappaport Vassiliadis broth<br />

20 x 10 ml . . . . . . . . . . Ref. 42073<br />

Rappaport Vassiliadis Soy broth<br />

20 x 10 ml . . . . . . . . . . Ref. 42110<br />

Selenite Cystine broth<br />

20 x 10 ml . . . . . . . . . . Ref. 42052<br />

Muller Kauffmann broth<br />

20 x 10 ml . . . . . . . . . . Ref. 42114<br />

MSRV medium<br />

6 x 200 ml . . . . . . . . . . Ref. 42639<br />

bioMérieux sa RCS Lyon 673 620 399 / Photos : N. Bouchut, Getty Images, Scorpius / Printed in France / TL. McCANN SANTÉ Lyon / RCS Lyon B 398 160 242 <strong>chromID</strong> <strong>Salmonella</strong> Agar<br />

+<br />

-<br />

* For availability, please consult us<br />

A solution to food industry constraints<br />

• Reliable results : the high level of sensitivity of SM ID 2<br />

avoids false negative results. <strong>chromID</strong> <strong>Salmonella</strong> : 100%,<br />

Other chromogenic medium : 93%, XLD : 89%. (Results<br />

obtained during an independent expert evaluation on 28<br />

matrices naturally and artificially contaminated and according<br />

to the NF EN ISO 6579 : 2002 protocol by combining the RVS<br />

and MKTTn results).<br />

• Cost savings : important reduction of confirmation tests due<br />

to <strong>chromID</strong> <strong>Salmonella</strong> specificity.<br />

• Adapted to real conditions of use : <strong>chromID</strong> <strong>Salmonella</strong><br />

has been evaluated on a large number of naturally and<br />

artificially contaminated food matrices.<br />

• Adapted to several protocols :<br />

- Detection of <strong>Salmonella</strong> in selective enrichment broths :<br />

Rappaport Vassiliadis Soy (RVS), Muller Kauffmann with<br />

Tetrathionate and novobiocin (MKTTn), Rappaport Vassiliadis<br />

(RV) and Selenite Cystine (SC) according to different<br />

standardized protocols (e.g., NF EN ISO 6579 : 2002).<br />

- Detection of <strong>Salmonella</strong> according to the VIDAS ® Immuno-<br />

Concentration <strong>Salmonella</strong> method (VIDAS ICS/plate method).<br />

- Confirmation of positive VIDAS <strong>Salmonella</strong> tests<br />

(VIDAS SLM).<br />

- Direct identification of suspect isolated colonies with API ® 20 E<br />

and ID 32 E strips.<br />

<strong>Salmonella</strong> Paratyphi A<br />

<strong>Salmonella</strong> Enteritidis + Proteus vulgaris

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