VIDAS UP E.Coli O157 (including H7) - bioMérieux - bioMerieux
VIDAS UP E.Coli O157 (including H7) - bioMérieux - bioMerieux
VIDAS UP E.Coli O157 (including H7) - bioMérieux - bioMerieux
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E. <strong>Coli</strong> <strong>O157</strong> (Including <strong>H7</strong>)<br />
Protocol for 50 to 375 g sample certified by AFNOR validation according to ISO 16140 N°<br />
BIO 12/25-05/09. Performance Tested by AOAC Research Institute (Certificate #060903)<br />
1 Detection 2 Confirmation<br />
500 μl from the BPW<br />
50 to 375 g of sample (raw beef & beef trim)<br />
Xg in 3X ml of BPW<br />
(Buffered<br />
Peptoned Water)<br />
Vancomycin<br />
(8 mg/L)<br />
8 - 24 hrs / 41.5°C ±1°C<br />
2 - 3 ml<br />
<strong>VIDAS</strong> ICE<br />
Results in 40 minutes<br />
500 μl<br />
Heat to<br />
95-100°C / 5±1min<br />
Using a calibrated swab,<br />
collect the immunoconcentrated solution of E. coli <strong>O157</strong><br />
from the first well of the <strong>VIDAS</strong> ICE strip<br />
Streak onto selective media<br />
<strong>O157</strong>:<strong>H7</strong> ID or CT-SMAC<br />
© 2009 BIOMÉRIEUX, INC. • BIOMÉRIEUX, THE BLUE LOGO AND <strong>VIDAS</strong> USED AND/OR REGISTERED AND PROTECTED TRADEMARKS BELONGING<br />
TO BIOMÉRIEUX SA OR ONE OF ITS SUBSIDIARIES • PRINTED IN U.S.A. VID-042-09<br />
bioMérieux, Inc.<br />
595 Anglum Rd<br />
Hazelwood, MO 63042<br />
USA<br />
Tel.: (800) 634 7656<br />
Test < 0.04<br />
No E. coli <strong>O157</strong> <strong>including</strong> <strong>H7</strong><br />
detected<br />
www.biomerieux.com<br />
www.biomerieux-industry.com<br />
<strong>VIDAS</strong> ® ECPT<br />
Results in 50 minutes<br />
bioMérieux, Canada, Inc.<br />
7815 Henri-Bourassa Blvd West<br />
St. Laurent (Québec)<br />
Canada H&S 1P7<br />
Tel.: (1) 514 336 7321<br />
Fax: (1) 514 807 0015<br />
Test ≥ 0.04<br />
CONFIRMATION with <strong>VIDAS</strong> ICE<br />
Incubate at 37°C ± 1°C / 18-24 hrs<br />
IDENTIFICATION OF SUSPECT COLONIES<br />
E.coli <strong>O157</strong> : <strong>H7</strong> range<br />
30 122 <strong>VIDAS</strong> <strong>UP</strong> E. coli <strong>O157</strong> (Including <strong>H7</strong>) 30 tests<br />
30 526 <strong>VIDAS</strong> ICE<br />
30 tests<br />
42 043 Buffered Peptoned Water 6 x 225 ml<br />
42 629 Buffered Peptoned Water 3 x 3 L<br />
42 042 Buffered Peptoned Water 6 x 90 ml<br />
42 729 Buffered Peptoned Water MINIBAGS 10 x 225 ml<br />
42 605 ChromID <strong>O157</strong>:<strong>H7</strong> 6 x 200 ml<br />
42 630 ChromID <strong>O157</strong>:<strong>H7</strong><br />
5 x 20 ml<br />
42 606 Cefixime-Tellurite 6 x 4 ml (lyo)<br />
* Consult us<br />
for availability<br />
For further<br />
details,<br />
consult<br />
the product<br />
package<br />
insert.
E. <strong>Coli</strong> <strong>O157</strong> (Including <strong>H7</strong>)<br />
Protocol for 25g sample certified by AFNOR validation according to ISO 16140 Nº BIO<br />
12/25-05/09. Performance Tested by AOAC Research Institute (Certificate #060903)<br />
1 Detection 2 Confirmation<br />
500 μl from the BPW<br />
25 g of sample (raw beef & beef trim)<br />
225 ml of BPW<br />
(Buffered<br />
Peptoned Water)<br />
7 - 24 hrs / 41.5°C ±1°C<br />
0.5 ml<br />
<strong>VIDAS</strong> ICE<br />
Results in 40 minutes<br />
Using a calibrated swab,<br />
collect the immunoconcentrated solution of E. coli <strong>O157</strong><br />
from the first well of the <strong>VIDAS</strong> ICE strip<br />
<strong>VIDAS</strong> ® Heat & Go / 5 ± 1 min<br />
Remove the strip and let it cool for 10 min<br />
Streak onto selective media<br />
<strong>O157</strong>:<strong>H7</strong> ID or CT-SMAC<br />
Incubate at 37°C ± 1°C / 18-24 hrs<br />
<strong>VIDAS</strong> ECPT<br />
Results in 50 minutes<br />
IDENTIFICATION OF SUSPECT COLONIES<br />
Test < 0.04<br />
No E. coli <strong>O157</strong> <strong>including</strong> <strong>H7</strong><br />
detected<br />
Test ≥ 0.04<br />
CONFIRMATION with <strong>VIDAS</strong> ICE
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