octet Platform

octet System Choices
Analyte MW
> 150 D
Analyte MW
> 5,000 D
SENSITIVITY
Label-Free Kinetic Characterization
all octet instruments provide sample temperature control from 4 °c above ambient up to 40 °c in 1 °c increments. the kinetic constant measurement
ranges are system- and assay-dependent. a typical range for each system is shown below.
octet SYSteMS Kinetic conStant rangeS
Application Specification Octet RED384 Octet QK384 Octet RED96 Octet QKe
Protein-Protein Kinetics ka, M -1 s -1 10 2 – 10 7 10 3 – 10 7 10 2 – 10 7 10 3 – 10 7
kd, s -1 10 -6 – 10 -1 10 -6 – 10 -1 10 -6 – 10 -1 10 -6 – 10 -1
KD, M 1 mM – 10 pM 0.1 mM – 10 pM 1 mM – 10 pM 0.1 mM – 10 pM
Protein-Small Molecule Kinetics ka, M -1 s -1 10 2 – 10 7 n/a 10 2 – 10 7 n/a
Data acquisition rate options Data points per
second (Hz)
Octet QK e
Octet RED96
• 96-well plates
• 1 sample plate position
• 8-channel readout
• Biosensor re-racking
THROUGHPUT
Octet QK384
• 96- and 384-well plates
• 2 sample plate positions
• 16-channel readout
• Automated plate handling
• Biosensor re-racking
• ≥40 µL/well (384TW microplate)*
kd, s -1 10 -6 – 10 -1 n/a 10 -6 – 10 -1 n/a
Octet RED384
*The 384TW microplate is a black, polypropylene, 384-well tilted
bottom plate designed for use on the Octet RED384 and Octet
QK384 instruments. The 384TW microplate enables use of sample
volumes as low as 40 µL, reduces background signal variability and
improves assay sensitivity.
KD, M 1 mM – 10 pM n/a 1 mM – 10 pM n/a
2, 5 and 10 Hz 0.3 and 0.6 Hz 2, 5 and 10 Hz 0.3 and 0.6 Hz