C1 - Fluidigm Corporation

COMPONENTS OF THE SINGLE-CELLC 1 AUTOPREP SYSTEMThe C 1 Single-Cell AutoPrep System isthe world’s first commercially available,automated single-cell isolation and preparationsystem for genomic analysis. TheC 1 System provides an easy and highly reproduciblegene expression workflow toprocess 96 single cells across 96 mRNAtargets. In this format, our single-cellworkflow enables you to measure theexpression of hundreds of genes in justa few hours compared to experimentsthat would normally take days using traditionalsystems. With this new systemyou can rapidly explore unique attributesof individual cells without the technicalvariability of a standard gene expressionworkflow.The C 1 Single Cell AutoPrep System consistsof the following:• n C 1 Single-Cell AutoPrep System—breakthrough,bench-top automation of isolation,lysis, and preamplification of single cells• n C 1 Single-Cell AutoPrep Array IFC—proprietary integrated fluidics circuit that facilitatescapture and highly paralleled preparationof 96 individual cells• n C 1 Single-Cell AutoPrep Reagent Kit—a ready-to-use reagent kit to support cellsuspension, lysis, and purificationUP TO 96 SINGLE CELLS IN JUST ONE HOURThe new C 1 Single-Cell AutoPrep System provides a simple “plugand play” workflow to reproducibly prepare 96 individual cells inapproximately 1 hour of hands-on time. The simple user interfaceand comprehensive C 1 System workflow helps you to:• n Capture cells—load batches of cells in a single pipetting step andrapidly separate into 96 individual chambers for preparation• n Verify—use an “in-process” quality control checkpoint to verify thenumber of captured cells and distinguish live and dead cells to preservedata integrity• n Lyse—use a rapid direct cell lysis method to save time and cost withoutRNA purification• n Reverse transcribe and preamplify—cDNA synthesis and specifictarget amplification occur on the same sample without reagent mixingand sample transfer• n Harvest—all preamplified products are pooled, harvested, and transferredto the BioMark HD System for quantitative PCR analysisThe C 1 Single-Cell AutoPrep System coupled with the BioMarkHD System streamlines gene expression analysis through seamlesseffort and significant time savings with support for up to96 individual cells across 96 transcripts. With automation andminimal costs the C 1 Single-Cell AutoPrep System dramaticallyincreases productivity to further accelerate expression profilingstudies. With an optimized protocol, pre-formulated reagent kit,and disposable arrays, you can achieve single-cell accuracy with“load and go” productivity.Capture Wash Verify Lyse RT PreAmp qPCR AnalyzeC 1 Single-Cell AutoPrep SystemBioMark HD SystemYour cells and your research are anything but average. Learn howthe C 1 Single-Cell AutoPrep System can put your gene expressionwork above the curve at fluidigm.com/c1system

fluidigm.com/c1system© 2012 Fluidigm Corporation. All rights reserved. Fluidigm, the Fluidigm logo, AutoPrep Array, BioMark, C 1 , DELTAgene, and Dynamic Array are trademarks or registeredtrademarks of Fluidigm Corporation in the U.S. and/or other countries. All other trademarks are the property of their respective owners.For Research Use Only. Not for use in diagnostic procedures.BR100-5477 A1 6/2012Corporate Headquarters7000 Shoreline Court, Suite 100South San Francisco, CA 94080 USAToll-free: 1.866.FLUIDLINE | Fax: 650.871.7152www.fluidigm.comSalesNorth America | +1 650.266.6170 | info-us@fluidigm.comEurope/EMEA | +33 1 60 92 42 40 | info-europe@fluidigm.comJapan | +81 3 3555 2351 | info-japan@fluidigm.comAsia | +1 650.266.6170 | info-asia@fluidigm.com