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EUROPEAN CENTRAL FORAGES DATABASES 19
Isozyme studies
In France, isozyme studies were performed on a sample of the European Lolium core
collection. So far, 38 populations (3 from Belgium, 3 from Switzerland, 3 from Ireland, 2
from The Netherlands, 1 from Norway, 1 from Czech Republic and 25 from France) have
been analyzed with two starch gels and two different buffer systems. This permitted us to
observe 12 readable loci. Another study on 120 other European populations gives an idea of
the genetic structuration of the diversity and the interest of carrying on such a study on the
European Lolium core collection.
Depending on funds available, France proposes to perform analyses on a subsample of
the European Lolium core collection, in order to draw maps of allelic distribution by means of
geostatistical analyses using both agronomic and isozyme results.
As most of the diversity is found within a population, it will be necessary to analyze
about 75 plants/population to observe allelic frequencies with good accuracy. For example,
the analysis of a subset of 80 populations requests about 12 man-months of labour (two
populations per week).