pathogenicity and aethiology of fusarium species ... - ePrints@USM

CHAPTER FIVE : PATHOGENICITY TEST OF Fusarium spp. IN
SECTION LISEOLA ON SUGARCANE
5.1 Introduction 54
5.2 Materials and methods
56
5.2.1 Source of sugarcane stalks
56
5.2.2 Inoculum and host preparation
56
5.2.3 Inoculation to healthy sugarcane plants
57
5.2.4 Disease assessment
58
5.2.5 Statistical analysis
58
5.2.6 Re-isolated and re-identified
59
5.3 Results
59
5.3.1 Symptoms development of pathogenic isolates
59
5.3.2 Disease severity index (DSI)
63
5.4 Discussion and Conclusion
69
CHAPTER SIX : VEGETATIVE COMPATIBILITY GROUPS OF F.
sacchari, F. subglutinans AND F. proliferatum
6.1 Introduction 73
6.2 Materials and methods
75
6.2.1 Fungal strains
75
6.2.2 Media
75
6.2.3 Generation of nitrate non-utilising (nit) mutants
75
6.2.4 Phenotyping of nit mutants
76
6.2.5 Complementation tests
78
6.3 Results
79
6.3.1 Generation of nit mutant
79
6.3.2 nit mutant phenotype
82
6.3.3 complementation tests of Nit mutants
84
6.3.4 Heterokaryon self-incompatible (HSI)
86
6.3.4.1 VCGs of F. sacchari
87
6.3.4.2 VCGs of F. subglutinans
90
6.3.4.3 VCGs of F. proliferatum
91
6.4 Discussion and conclusion
92
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