Amersham ECL Prime specifications

GE Healthcare
Life Sciences
New Amersham TM ECL TM Prime
The latest addition to the Amersham ECL family, Amersham ECL Prime consolidates and builds on the
benefits of Amersham ECL Plus and Amersham ECL Advance to deliver a detection system that is sensitive,
stable, quantitative, and conservative in its consumption of expensive antibody reagents.
Improved sensitivity with ECL Prime
Amersham ECL Prime Amersham ECL Plus
2.5 ng 2.4 pg 2.5 ng 9.8 pg
Fig 1. Western blotting detection of transferrin in a 2-fold dilution series using Amersham
ECL Prime (left) and Amersham ECL Plus (right). The samples were blotted onto an Amersham
Hybond-P (PVDF) membrane and imaged using an ImageQuant LAS 4000 mini system.
Amersham ECL Prime features and benefits
Sensitivity and precision Detects both high and low abundant
proteins as well as phosphorylated
proteins
High intensity and
stability
Quantitative detection
of target proteins
Efficient in the use of
precious antibodies
Convenient handling and multiple
exposures of the same blot due to
long lasting signals
Optimal for protein quantitation due
to broad dynamic range
Detects low quantities of proteins
even when highly diluted antibodies
are used
Amersham ECL Prime
Horseradish peroxidase
(HRP)-linked secondary
antibody
Primary antibody
Target protein
HRP
Chemiluminescence
Fig 2. The reaction between Amersham ECL Prime
reagent and HRP linked to a secondary antibody. HRP
catalyzes the conversion of Amersham ECL Prime
reagent to a sensitized molecule, which on further
oxidation produces an excited product that emits light
when it decays.

Amersham ECL Prime specifications
Detection limit ~ 2 pg
Signal duration Up to 3 h, using constant exposure time
Working solution stability 24 h at room temperature
Stock solution shelf life 12 mo at 4-8°C
Recommended dilution range
for primary antibody
1:1000 -1:50000
Recommended dilution range
for secondary antibody
1:50000 - 1:250000
Examples of application • Detection and quantitation of posttranslational modifications
• Detection of different protein levels as a response to treatment
Ordering information
Product VWR Cat. No.
Amersham ECL Prime Western Blotting Detection 89168-782
Reagents for 1000 cm 2 membrane 1
1 Includes Solution A (luminol solution, 50 ml) and Solution B (peroxide solution, 50 ml),
sufficient for 1000 cm 2 membrane.
Related products 2 VWR Cat. No.
Amersham Hybond-P (20 x 20 cm), 10 sheets 95038-368
ECL Prime Blocking Agent, 20 g 95040-068
ECL Human IgG, HRP-Linked Whole Ab 95017-326
(from sheep), 1ml
ECL Rabbit 1gG, HRP-Linked Whole Ab 95017-556
(from donkey), 1ml
Full-Range Rainbow Molecular Weight 95040-114
Markers 250 µl
ECL DuaVue Western Blotting Markers 95038-574
(25 loadings)
2 For a more comprehensive list of related products, please refer to www.gelifesciences.com/ecl
Please ask your local sales representative about
receiving a sample of ECL Prime for evaluation
GE, imagination at work, and GE monogram are trademarks of General Electric Company.
Amersham and ECL are trademarks of GE Healthcare companies.
© 2011 General Electric Company—All rights reserved.
First published November 2009.
0311 Lit. No. POD 07061