AFIA - Laboratory Methods Manual - Australian Fodder Industry ...

More documents

Recommendations

Info

References: National Forage Testing Association - Forage Analysis Procedures 3.3 Nitrogen Determination by Combustion Method. Protein (Crude) in Animal Feed: Combustion Method. (990.03) Official Methods of Analysis. 1990. Association of Official Analytical Chemists. 15th Edition. AFIA Laboratory Methods Manual – v7 September 2011 Page 26 of 103
Version 1 Date 31/08/05 Method – 1.6R: Determination of the Ammonia-N Content of Silages Scope: This method determines the ammonia-N content of silages to evaluate silage fermentation quality. Principle: An acid extract is prepared from a chopped silage sample stored in a sealed container in a cold room for a period of at least 24 hours. Ammonia-N is absorbed into the solution as ammonium ion. There are various methods available for determining the ammonia-N concentration in an aliquot of the filtrate from the silage extract. The following procedure is based on an automated distillation and titration method (Method 1.4R), where the ammonium ion is converted back to ammonia and the titrant volume is recorded for calculation of the result, which is expressed as a % of the total N. An alternative method (1.15A) determines ammonia-N using an ion specific electrode. Apparatus: Volumetric flask (5L) Fume hood with neutralising tank for acidic vapours Digestion tubes Digestion tube stand Steam distillation apparatus Kjeltec Analyser or equivalent) Analytical electronic balance, accurate to 0.1 mg Forceps Spatula Variable dispenser - 25ml volume Conical flasks, Glass 250ml Burette Protective gloves Reagents: Sodium Hydroxide (NaOH) ⎯technical grade, (35−40%) Sulphuric acid (H2SO4)⎯0.3 M Boric acid (H3BO3) Bromocresol green Indicator Methyl red Indicator Sodium carbonate (Na2CO3) Hydrochloric acid⎯32% HCl (or 36%), or alternatively use 0.1N sulphuric acid as per Method 1.4R Methanol Water - Type II AFIA Laboratory Methods Manual – v7 September 2011 Page 27 of 103
Page 1 and 2: AFIA - Laboratory Methods Manual A
Page 3 and 4: FOREWORD This manual is the result
Page 5 and 6: TABLE OF CONTENTS SECTION 1: AFIA R
Page 7 and 8: SECTION 1 AFIA REFERENCE AND APPROV
Page 9 and 10: Sample Coning and Quartering: This
Page 11 and 12: Version 2 Date 31/08/05 Method - 1.
Page 13 and 14: l) Grind the coarse material in a c
Page 15 and 16: Procedure: Step1. Partial dry matte
Page 17 and 18: Appendix 1.3R(1): Method for Silage
Page 21 and 22: Procedure: Note: Analyses are usual
Page 23 and 24: Appendix 1.4R(1): Determining the C
Page 25: 7. Include two blanks and three dri
Page 29 and 30: 4. Store the sample in a refrigerat
Page 31 and 32: Version 4 Date 22/05/2011 Method -
Page 33 and 34: 4. Weigh out unknown samples and in
Page 35 and 36: Correction for silage DMD slope For
Page 37 and 38: Preparing an aNDF Solution: a) Pour
Page 39 and 40: Comments: • Difficult filtration
Page 41 and 42: Appendix 1.8R(1): NFTA Method 5.2:
Page 43 and 44: Comments: • The volume of the amy
Page 47 and 48: 8. Add 2000 mL of ambient temperatu
Page 51 and 52: 10. When the reagent starts to boil
Page 53 and 54: Undersander D, Mertens DR, Thieux N
Page 55 and 56: 14. Determine the number (n) of sam
Page 57 and 58: Procedure: 1. Samples should be mic
Page 59 and 60: Appendix 1.9R(1): Differences in th
Page 61 and 62: Normality MUST be verified by titra
Page 63 and 64: Van Soest PJ, Robertson JB and Lewi
Page 65 and 66: Normality MUST be verified by titra
Page 67 and 68: Goering HK and van Soest PJ (1970).
Page 69 and 70: • Slide the desiccator lid open.
Page 71 and 72: Funnels, plastic Procedure: 1. Weig
Page 73 and 74: Appendix 1.11R(1): Notes on the Ant
Page 77 and 78:
Make up all working standards to vo
Page 79 and 80:
Figure 1 Carrier/water Blue/Blue 2.
Page 81 and 82:
Store the electrode in 0.1M KCl sol
Page 83 and 84:
Laboratory samples must be equilibr
Page 85 and 86:
Safety Precautions: • There shoul
Page 87 and 88:
SECTION 2 AFIA REFERENCE PROCEDURES
Page 89 and 90:
Version 2 Date 31/08/05 Method - 2.
Page 91 and 92:
Method - 2.2R: Calculation of Metab
Page 93 and 94:
Appendix 2.2R(1). Calculation of Or
Page 95 and 96:
SECTION 3 DRAFT METHODS Draft metho
Page 97 and 98:
Reagents: 1. Stock Solution A. Ther
Page 99 and 100:
Calculations: Percent Starch on DM
Page 101 and 102:
5. Sodium hydroxide solution, 10M.
Page 103:
Acceptance of results: The r 2 valu
show all

AFIA - Laboratory Methods Manual - Australian Fodder Industry ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?