AFIA - Laboratory Methods Manual - Australian Fodder Industry ...

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The calculation that follows is for dilution of an enzyme stock solution or extraction to allow 2ml of standardised amylase solution to be added during the aNDF procedure. Where: Reference: E = 100 x (Vs X C) / 2 E = Enzyme powder (g) or liquid (ml) needed to make 100ml of standardised amylase solution Vs = Minimum volume of test solution or extract resulting in yellow colour with iodine (no starch) C = Concentration of Amylase Test Solution (g powder/100ml or ml liquid/100ml) 2ml = Volume to be added in aNDF procedure 100ml = Volume of standardised amylase solution to be prepared D. R. Mertens, 1992, USDA Dairy Forage Research Center, Personal communication. AFIA Laboratory Methods Manual – v7 September 2011 Page 44 of 103
Version 3 Date 22/05/2011 Method – 1.8A(a): Determination of Amylase Neutral Detergent Fibre - Ankom Scope: This procedure describes the use of Ankom equipment to determine Neutral Detergent Fibre in feed samples which have been ground to pass a 1 mm screen. Principle: Neutral Detergent Fibre (NDF) is considered to represent plant cell-wall constituents including hemicellulose, cellulose, lignin, cutin and silica. The sample is weighed into filter bags and refluxed in hot neutral detergent solution for 1 hour. It is then rinsed, dried and weighed. The amount of sample remaining is the NDF and is expressed as a percentage dry matter (%DM). Apparatus: ANKOM 200/220 Fibre Analyser Bag Suspender with Baskets Analytical Balance Computer Spatula Filter Bags, ANKOM #57 Measuring Cylinder, Glass 1 L Measuring Cylinder, Glass 10 mL Electric Urn, 6 L capacity or ZIP Boil instant hot water service or equivalent Beakers, Glass 250 mL and 2 L Oven, 105 o C Heat Sealer Timer Gloves AFIA Laboratory Methods Manual – v7 September 2011 Page 45 of 103
Page 1 and 2: AFIA - Laboratory Methods Manual A
Page 3 and 4: FOREWORD This manual is the result
Page 5 and 6: TABLE OF CONTENTS SECTION 1: AFIA R
Page 7 and 8: SECTION 1 AFIA REFERENCE AND APPROV
Page 9 and 10: Sample Coning and Quartering: This
Page 11 and 12: Version 2 Date 31/08/05 Method - 1.
Page 13 and 14: l) Grind the coarse material in a c
Page 15 and 16: Procedure: Step1. Partial dry matte
Page 17 and 18: Appendix 1.3R(1): Method for Silage
Page 21 and 22: Procedure: Note: Analyses are usual
Page 23 and 24: Appendix 1.4R(1): Determining the C
Page 25 and 26: 7. Include two blanks and three dri
Page 29 and 30: 4. Store the sample in a refrigerat
Page 31 and 32: Version 4 Date 22/05/2011 Method -
Page 33 and 34: 4. Weigh out unknown samples and in
Page 35 and 36: Correction for silage DMD slope For
Page 37 and 38: Preparing an aNDF Solution: a) Pour
Page 39 and 40: Comments: • Difficult filtration
Page 41 and 42: Appendix 1.8R(1): NFTA Method 5.2:
Page 43: Comments: • The volume of the amy
Page 47 and 48: 8. Add 2000 mL of ambient temperatu
Page 49 and 50: Version 3 Date 22/05/2011 Method -
Page 51 and 52: 10. When the reagent starts to boil
Page 53 and 54: Undersander D, Mertens DR, Thieux N
Page 55 and 56: 14. Determine the number (n) of sam
Page 57 and 58: Procedure: 1. Samples should be mic
Page 59 and 60: Appendix 1.9R(1): Differences in th
Page 61 and 62: Normality MUST be verified by titra
Page 63 and 64: Van Soest PJ, Robertson JB and Lewi
Page 65 and 66: Normality MUST be verified by titra
Page 67 and 68: Goering HK and van Soest PJ (1970).
Page 69 and 70: • Slide the desiccator lid open.
Page 71 and 72: Funnels, plastic Procedure: 1. Weig
Page 73 and 74: Appendix 1.11R(1): Notes on the Ant
Page 77 and 78: Make up all working standards to vo
Page 79 and 80: Figure 1 Carrier/water Blue/Blue 2.
Page 81 and 82: Store the electrode in 0.1M KCl sol
Page 83 and 84: Laboratory samples must be equilibr
Page 85 and 86: Safety Precautions: • There shoul
Page 87 and 88: SECTION 2 AFIA REFERENCE PROCEDURES
Page 91 and 92: Method - 2.2R: Calculation of Metab
Page 93 and 94: Appendix 2.2R(1). Calculation of Or
Page 95 and 96:
SECTION 3 DRAFT METHODS Draft metho
Page 97 and 98:
Reagents: 1. Stock Solution A. Ther
Page 99 and 100:
Calculations: Percent Starch on DM
Page 101 and 102:
5. Sodium hydroxide solution, 10M.
Page 103:
Acceptance of results: The r 2 valu
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