honeybush research programme publications - Agricultural ...

HONEYBUSH RESEARCH PROGRAMME PUBLICATIONS (ARC & COLLABORATORS)
SCIENTIFIC PUBLICATIONS
(PREVIOUS UPDATE: OCTOBER 2008, LAST UPDATE: FEBRUARY 2011)
COETZEE, G., MARX, I.J., PENGILLY, M., BUSHULA, E., BLOOM, M., 2008. Effect of rooibos and honeybush
tea extracts against Botrytis cinerea. South African Journal of Enology and Viticulture, 29, 8-13.
DE BEER, D., JERZ, G., JOUBERT, E., WRAY, V., WINTERHALTER, P., 2009. Isolation of isomangiferin from
honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance
liquid chromatography.Journal of Chromatography, 1216, 4282-4289.
DE BEER, D., JOUBERT, E., 2010. Development of HPLC method for Cyclopia subternata phenolic compound
analysis and application to other Cyclopia spp. Journal of Food Composition and Analysis, 23, 289-297.
DU TOIT, J. & JOUBERT, E., 1998. The effect of pretreatment on the fermentation of honeybush tea (Cyclopia).
Journal of the Science of Food and Agriculture, 76, 537-545.
DU TOIT, J. & JOUBERT, E., 1998. Effect of drying conditions on the quality of honeybush tea (Cyclopia). Journal
of Food Processing and Preservation, 22, 493-507.
DU TOIT, J. & JOUBERT, E., 1999. Optimization of the fermentation parameters of honeybush tea (Cyclopia).
Journal of Food Quality, 22, 241-256.
DU TOIT, J., JOUBERT, E. & BRITZ, T.J., 1999. Identification of microbial contaminants present during the curing
of honeybush tea (Cyclopia). Journal of the Science of Food and Agriculture, 79, 2040-2044.
DU TOIT, J., JOUBERT, E. & BRITZ, T.J., 1998. Honeybush tea - a rediscovered South African herbal tea. Journal
of Sustainable Agriculture, 12, 67-84.
FERREIRA, D., KAMARA, B.I., BRANDT, E.V & JOUBERT, E., 1998. Phenolic compounds from Cyclopia
intermedia (Honeybush tea) - I. Journal of Agricultural and Food Chemistry, 46, 3406-3410.
JOUBERT, E., MANLEY, M. & BOTHA, M., 2006. Use of NIRS for quantification of mangiferin and hesperidin
contents of dried green honeybush (Cyclopia genistoides) plant material. Journal of Agriculture and Food
Chemistry, 54, 5279-5283.
JOUBERT, E., OTTO, F., GRUNER, S. & WEINREICH, B., 2003. Reversed-phase HPLC determination of
mangiferin, isomangiferin and hesperidin in Cyclopia and the effect of harvesting date on the phenolic
composition of C. genistoides. European Food Research and Technology, 216, 270-273.
JOUBERT, E., MANLEY, M., BOTHA, M., 2008. Evaluation of spectrophotometric methods for screening of green
rooibos (Aspalathus linearis) and green honeybush (Cyclopia genistoides) extracts for high levels of bioactive
compounds. Phytochemical Analysis, 19, 169-178.
JOUBERT, E., RICHARDS, E.S., VAN DER MERWE, J.D. DE BEER, D., MANLEY, M., GELDERBLOM, W.C.A.,
2008. Effect of species variation and processing on phenolic composition and in vitro antioxidant activity of
aqueous extracts of Cyclopia spp. (honeybush tea). Journal of Agricultural and Food Chemistry, 56, 954-
963.
JOUBERT, E., GELDERBLOM, W.C.A., LOUW, A. & DE BEER, D., 2008. South African herbal teas: Aspalathus
linearis, Cyclopia spp. and Atrixia phylicoides - A review. Journal of Ethnopharmacology, 119 (2008) 376-
412.
JOUBERT, E., GELDERBLOM, W.C.A., DE BEER, D., 2009. Phenolic contribution of South African herbal teas to
a healthy diet. Natural Product Communications, 4, 701-718.
KAMARA, B.I., BRANDT, E.V., FERREIRA, D. & JOUBERT, E., 2003. Polyphenols from Honeybush Tea (Cyclopia
intermedia) . 2. Journal of Agricultural and Food Chemistry, 51, 3874-3879.
KAMARA, B.I., BRAND, J., BRANDT, E.V., & JOUBERT, E., 2004. Phenolic metabolites from honeybush tea
(Cyclopia subternata). Journal of Agricultural and Food Chemistry, 52, 5391-5395.
LE ROUX, M., CRONJE, J.C., JOUBERT, E., BURGER, B.V., 2008. Chemical characterisation of the constituents
of the aroma of honeybush, Cyclopia genistoides. SA Journal of Botany, 74, 139-143.
MARNEWICK, J.L., GELDERBLOM, W.C.A. & JOUBERT, E., 2000. An investigation on the antimutagenic
properties of South African herbal teas. Mutation Research, 471, 157-166.
MARNEWICK, J.L., JOUBERT, E., SWART, P., VAN DER WESTHUIZEN, F. & GELDERBLOM, W., 2003.
Modulation of hepatic drug metabolising enzymes and oxidative status by unique South African herbal teas,
rooibos (Aspalathus linearis) and honeybush (Cyclopia intermedia), green and black (Camellia sinensis)
teas in rats. Journal of Agricultural and Food Chemistry, 51, 8113-8119.
MARNEWICK, J.L., BATENBURG, W., SWART, P., JOUBERT, E., SWANEVELDER, S., & GELDERBLOM, W.,
2004. Ex vivo modulation of chemical-induced mutagenesis by subcellular liver fractions of rats treated with
rooibos (Aspalathus linearis) tea, honeybush (Cyclopia intermedia) tea, was well as green and black
(Camellia sinensis) teas. Mutation Research, 558, 1454-154.
MARNEWICK, J.L., JOUBERT, E., JOSEPH, S., SWANEVELDER, S., SWART, P. & GELDERBLOM, W.C.A.,
2005. Inhibition of tumour promotion in mouse skin by extracts of rooibos (Aspalathus linearis) and
honeybush (Cyclopia intermedia), unique South African. Cancer Letters, 224, 193-202.
MARNEWICK, J.L, VAN DER WESTHUIZEN, F.H., JOUBERT, E., SWANEVELDER, S., SWART, P.,
GELDERBLOM, W.C.A., 2009. Chemoprotective properties of rooibos (Aspalathus linearis), honeybush

(Cyclopia intermedia), green and black (Camellia sinensis) teas against cancer promotion induced by
fumonisin B 1 in rat liver.Food and Chemical Toxicology, 47, 220-229.
MFENYANA, C., DE BEER, D., JOUBERT, E., LOUW, A., 2008. Selective extraction of Cyclopia for enhanced in
vitro phytoestrogenicity and benchmarking against commercial phytoestrogen extracts. The Journal of
Steroid Biochemistry and Molecular Biology, 112, 74-86.
VAN DER MERWE, J.D., JOUBERT, E., RICHARDS, E., MANLEY, M, SNIJMAN, P.W., MARNEWICK, J.L.&
GELDERBLOM, W.C.A., 2006. A comparative study on the antimutagenic properties of aqueous extracts of
Aspalathus linearis (rooibos), different Cyclopia spp. (honeybush) and Camellia sinensis teas. Mutation
Research, 611, 42-53.
VERHOOG, N.J.D., JOUBERT, E., LOUW, A., 2007. Screening of four Cyclopia (honeybush) species for putative
phytoestrogenic activity through estrogen receptor binding assays. SA Journal of Science, 103, 13-21.
VERHOOG, N.J.D., JOUBERT, E., LOUW, A., 2007. Evaluation of the phytoestrogenic activity of Cyclopia
genistoides (Honeybush) methanol extracts and relevant polyphenols. Journal of Agricultural and Food
Chemistry, 55, 4371-4385.
SEMI-SCIENTIFIC PUBLICATIONS
JOUBERT, M., KOTZÉ, W.A.G. & WOOLDRIDGE, J., 2007. Effect of liming and mineral nutrition on growth of
honeybush (Cyclopia spp.) plants. South African Journal of Plant and Soil, 24 (3) 161-165.
JOUBERT, M., BOTMA, P.S., KOTZÉ, W.A.G. & WOOLDRIDGE, J., 2007. Honeybush (Cyclopia spp.) response to
phosphorus fertilization and mulching. South African Journal of Plant and Soil, 24 (3) 176-177.
THESES
BOTHA, M., 2005. Use of NIRS in quality control of rooibos and honeybush. MSc thesis, University of
Stellenbosch.
BRAND, D.J., 2002. Structure and synthesis of polyphenols from Cyclopia subternata. MSc thesis, University of
Free State, Bloemfontein.
CRONJE, J.C., 2006. Chemiese karakterisering van aroma van die heuningbosspesie Cyclopia genistoides. MSc
thesis, Universiteit van Stellenbosch.
CRONJE, J.C., 2010.Chemical characterisation of the aroma of honeybush (Cyclopia) species, Stellenbosch
University (Ph.D, Chemistry).
DU TOIT, J., 1997. Development of standardised processing method for honeybush tea (Cyclopia). MSc thesis,
University of Stellenbosch.
HUBBE, M.E., 2000. Evaluation of the antioxidant and free radical scavenging properties of honeybush tea
(Cyclopia), MSc thesis, University of Stellenbosch.
KAMARA B.I., 1997. Structure and synthesis of phenolic metabolites from honeybush tea (Cyclopia intermedia).
M.Sc thesis, University of Orange Free State, Bloemfontein.
KAMARA B.I., 1999. Structure and synthesis of polyphenols from honeybush tea (Cyclopia intermedia) and the
potential of flavonoids as active scavengers. Ph D thesis, University of Orange Free State, Bloemfontein.
MAICU, C., 2007. Optimisation of retention of mangiferin in green Cyclopia subternata during preparation for drying
and storage and development of NIRS calibration models for rapid quantification of mangiferin and
hesperidin contents, Stellenbosch University (M.Sc. Food Science)
MFENYANA, C., 2007.Selective extraction of Cyclopia for enhanced in vitro phytoestrogenicity, Stellenbosch
University (M.Sc. Biochemistry).
RICHARDS, E.S., 2002 Antioxidant and antimutagenic properties of Cyclopia species. MSc thesis, University of
Stellenbosch.
SISSING, L., 2007. Investigation into the cancer modulating properties of Aspalathus linearis (rooibos), Cyclopia
intermedia (honeybush) and Sutherlandia frutescens (cancer bush) in oesphophagael carcinogenesis.
M.Sc.(Medical Bioscience), University of the Western Cape, South Africa.
VAN DER MERWE, J.D., 2004. Antimutagenic activity of rooibos, honeybush, green and black teas against
aflatoxin B1 and possible mechanisms involved. MSc thesis, University of Stellenbosch.
VERHOOG, N.J.D., 2005. Phytoestrogen activity of honeybush (Cyclopia spp.). MSc thesis, University of
Stellenbosch.
PUBLISHED CONGRESS PROCEEDINGS
HUBBE, M.E. & JOUBERT, E., 2000. In vitro suoperoxide anion radical scavenging ability of honeybush tea
(Cyclopia). In: Dietary Anticarcinogens and Antimutagens. Chemical and Biological Aspects (edited by I.T.

Johnson & G.R. Fenwick). Royal Chemistry Society, UK, pp.242-244 (Proceedings of the Food and Cancer
Prevention III Meeting, 5-8 September 1999).
HUBBE, M.E. & JOUBERT, E., 2000. Hydrogen donating ability of honeybush tea (Cyclopia intermedia) as a
measure of antioxidant activity. Polyphenol Communications 2000 (Proceedings of the 20 th International
Conference on Polyphenols), 361-362 (edited by S. Martens, D. Treutter & G. Forkmann), TUM.
MARNEWICK, J.L., GELDERBLOM, W.C.A. & JOUBERT, E., 2000. Antimutagenicity of South African herbal teas.
In: Dietary Anticarcinogens and Antimutagens. Chemical and Biological Aspects (edited by I.T. Johnson &
G.R. Fenwick). Royal Chemistry Society, UK, pp.384-385 (Proceedings of the Food and Cancer Prevention
III Meeting, 5-8 September 1999).
POPULAR PUBLICATIONS
ANON, 2001. Friemersheimers oes heuningbos. Landbou Burger, 7 Desember (na onderhoud met Marlise
Joubert en Philip Botma).
BEUKES, O., 2001. Besproeiing van heuningbos. SAHPA-nuusbrief no 3 (April), 2 pp.
BLOEM, J., 2001. Biologiese stikstofbinding deur gebruik van heuningbos entstof. SAHPA-nuusbrief no 4, (Julie),
4 pp.
CARSTENS, R., 2004. LNR Infruitec-Nietvoorbij bestuur besoek heuningbosboere. SAHTA Nuusbrief no 10
(Julie), 1 pp.
COETZEE, J., 1999. Heuningbos word ‘n volwaardige bedryf. Landbouweekblad, 20 Augustus.
COETZEE, J., 2000. Heuningbostee sprei sy soet geur. Landbouweekblad 14 Januarie 2000. (na onderhoud met
Marlise Joubert en Philip Botma).
DE LANGE, H. & BOTMA, P., 2000. Hoe om heuningbos plantjies te maak. SAHPA Nuusbrief no 2 (November), 8
pp.
DE LANGE, H. 2002. Geskiedenis en ontwikkeling van heuningbostee. SAHTA nuusbrief no 6 (Junie), 7 pp.
DE LANGE, H. 2005. Heuningbosteebedryf: Oorwegings ten opsigte van genetiese material en metodes van
voortplanting. SAHTA Nuusbrief No. 13 (Oktober), 5 pp.
DE LANGE, H., 2006. Huidige stand van plantverbetering in die heuningbostee-bedryf. SAHTA nuusbrief 14
(Oktober), 8 pp.
FOURIE, J. & JOUBERT, M., 2003. Organiese onkruidbeheer in heuningbos aanplantings. SAHTA nuusbrief no 8
(Julie), 7 pp.
ISAACS, J., 2001. Honeybush tea: technology transfer through demonstration plots to resource-limited producers.
SAHPA-newsletter no 4 (July), 3 pp.
JOUBERT, E., 2001. Honeybush tea and health: Honeybush tea as an antioxidant-rich beverage. SAPHA
Newsletter, No. 4 (July), 2 pp.
JOUBERT, E., 2002. Honeybush tea and health: Bergtee or vleitee? SAHTA Newsletter No. 5 (February), 2 pp.
JOUBERT, E., 2002. NRF supports honeybush tea research. SAHTA newsletter no 6 (June), 1 pp.
JOUBERT, E. & DU TOIT, J., 1997. Honeybush tea. Intermediate Technology, Food Chain, Nr 20, 10-12.
JOUBERT, E. & DU TOIT, J., 1997. The taste of honey. SPORE, No. 71, 7.
JOUBERT, E. & GELDERBLOM, W.C.A. (2002). Honeybush tea and health: Substantiation of health claims –
where are we now? SAHTA Newsletter No. 6, 2 pp.
JOUBERT, E. & MARNEWICK, J.L., 2003. Honeybush tea and health: Honeybush tea and cancer prevention.
SAHTA Newsletter No.8, 2 pp.
JOUBERT, E., 2003. Who’s ahead in teas? Food and Beverage Reporter, Nov/Dec, p. 26. (also abstracted in
Farmer’s Weekly, 16 Jan 2004, p. 19).
JOUBERT, E., GELDERBLOM, W., LOUW, A., The promise of honeybush. SA Vegetables & Fruit, June/July,
2008. pp 22-23.
JOUBERT, M. (ed), 2000 SAHPA Nuusbrief – Newsletter no 1 (Maart).
JOUBERT, M., 2000. Statistiek van heuningbos bedryf. SAHPA nuusbrief no 1, (Maart), 1pp.
JOUBERT, M. (ed), 2000. SAHPA Nuusbrief – Newsletter no 2, (November).
JOUBERT, M., 2000. Hoe kies ek ‘n geskikte area en grond vir ‘n heuningbos aanplanting? SAHPA-nuusbrief no
2 (November), 5pp .
JOUBERT, M. (ed), 2001. SAHPA Nuusbrief – Newsletter no 3, (April).
JOUBERT, M., 2001. Hoekom moet ek grond voorberei voor plant van heuningbos? SAHPA-nuusbrief no 3,
(April), 3 pp.
JOUBERT, M., 2001. Hoekom moet ek grond voorberei voor plant van heuningbos? Nufarmer & African
Entrepreneur 6: 1.
JOUBERT, M. (ed), 2001. SAHPA Nuusbrief – Newsletter no 4, (Julie).
JOUBERT, M., 2001. How to take soil samples. SAHPA-newsletter no 4, (July), 2 pp.
JOUBERT, M. (ed), 2002. SAHPA Nuusbrief – Newsletter no 5, (February).
JOUBERT, M., 2002. Nuusbrokkies van die LNR. SAHPA Nuusbrief no 5, (Februarie), 1 pp.

JOUBERT, M. (ed), 2002. SAHTA Nuusbrief – Newsletter no 6, (June).
JOUBERT, M. (ed), 2002. SAHTA Nuusbrief – Newsletter no 7, (November).
JOUBERT, M., 2002 Wenke en hektare aangeplant. SAHTA Nuusbrief no 7 (November), 2 pp.
JOUBERT, M. (ed), 2003. SAHTA Nuusbrief – Newsletter no 8, (July).
JOUBERT, M. (ed), 2004. SAHTA Nuusbrief – Newsletter no 9, (February).
JOUBERT, M., 2004. Vergadering tussen LNR Infruitec-Nietvoorbij, SAHTA dagbestuur en Departement Landbou:
Wes-Kaap. SAHTA Nuusbrief – Newsletter no 9, (Februarie), 4 pp.
JOUBERT, M., 2004. Samewerking met Departement Landbou: Wes-Kaap. SAHTA Nuusbrief no 9 (Februarie), 1
pp.
JOUBERT, M., 2004. Besoeke aan produsente. SAHTA Nuusbrief no 9 (Februarie), 1 pp.
JOUBERT, M., 2004. Besoek aan Nortje’s van Nooitgedacht. SAHTA Nuusbrief no 9, (February), 1 pp.
JOUBERT, M. (ed), 2004. SAHTA Nuusbrief – Newsletter no 10, (July).
JOUBERT, M., 2004. Befondsing van projekte. SAHTA Nuusbrief no 10, (Julie), 1 pp.
JOUBERT, M., 2004. Navorsing oor verbouiingsaspekte van heuningbos. SAHTA Nuusbrief no 10, (Julie), 1 pp.
JOUBERT, M., 2004. Wenke. SAHTA Nuusbrief no 10, (Julie), 1 pp.
JOUBERT, M. (ed), 2004. SAHTA Nuusbrief – Newsletter no 11 (November).
JOUBERT, M., 2004.. Uittreksels van lesings by tegniese dag – Julie 2004. SAHTA Nuusbrief – Newsletter no 11
(November), 2 pp.
JOUBERT, M., 2004. Nuusbrokkies. SAHTA Nuusbrief no 11 (November), 1 pp.
JOUBERT, M. (ed), 2005. SAHTA Nuusbrief – Newsletter no 12 (April).
JOUBERT, M. (ed), 2005. SAHTA Nuusbrief – Newsletter no 13 (October).
JOUBERT, M., 2005. Nuusbrokkies. SAHTA Nuusbrief – Newsletter no 13 (October), 2 pp.
JOUBERT, M. (ed), 2006. SAHTA Nuusbrief – Newsletter no 14 (June).
JOUBERT, M. & BOTMA, P., 2001. Vestiging van ‘n heuningbos aanplanting. SAHPA-nuusbrief no 3 (April), 3 pp.
JOUBERT, M. & BOTMA, P., 2001. Vestiging van ‘n heuningbos aanplanting. Nufarmer & African Entrepreneur 6,
5 (15/7/2001)
JOUBERT, M., JOUBERT E, VON MOLLENDORFF, L., NORTH M. & DE LANGE H., 2008. Honeybush tea – a
herbal tea. S.A. Fruit Journal, 54, Feb/March, 54-55.
JOUBERT M., JOUBERT, E. & DE LANGE, H., 2008. Honeybush production in the Western and Eastern Cape
Provinces. SA Vegetables & Fruit, No 122 (April – May), 18-19.
LOTZ, E., 2002. Europgap, en die implikasies vir die heuningbostee produsent. SAHTA nuusbrief no 6 (Junie), 1
pp.
LOUW , A., & JOUBERT, E., 2006. The good, the bad (and the alternative)- Estrogens vs phytoestrogens.
SAHTA Newsletter No.14, (15 Jun 06), 2 pp.
LOUW , A., VERHOOG, N. & JOUBERT, E., 2005. Honeybush tea and health: Helping to alleviate menopausal
symptoms – Old wives tales or truth in science? SAHTA Newsletter No.12, 2 pp.
MARNEWICK, J.L., JOUBERT, E., 2004. Honeybush tea and health: Honeybush tea and cancer prevention.
SAHTA Newsletter No.11, 2 pp.
VON MOLLENDORFF, L.J., DE LANGE, H., 2001. The development and commercialisation of ARC Infruitec-
Nietvoorbij deciduous fruit and honeybush cultivars within and outside South Africa. SAPHA-newsletter no
4 (July), 7 pp.
VON MOLLENDORFF, L.J., 2002. Heuningbos teelprogram van LNR Infruitec-Nietvoorbij kultivars. SAPHAnuusbrief
no 5 (Februarie), 1 pp.
VON MOLLENDORFF, L.J., 2002. Kommersiële pad van beskermde LNR heuningbos kultivars. SAPHAnuusbrief
no 5 (Februarie), 1 pp.
SAHTA NEWSLETTERS (downloads)
The last SAHTA newsletter was published in October 2008. General information can now be accessed on the
SAHTA website (www.sahoneybush.co.za)
SAHPA Newsletter 1 March 2000
SAHPA Newsletter 2 November 2000
SAHPA Newsletter 3 April 2001
SAHPA Newsletter 4 July 2001
SAHPA Newsletter 5 February 2002
SAHTA Newsletter 6 June 2002
SAHTA Newsletter 7 November 2002
SAHTA Newsletter 8 July 2003
SAHTA Newsletter 9 February 2004
SAHTA Newsletter 10 July 2004
SAHTA Newsletter 11 November 2004
SAHTA Newsletter 12 April 2005
SAHTA Newsletter 13 October 2005

SAHTA Newsletter 14 June 2006
SAHTA Newsletter 15 October 2008