bnf in sugarcane, ghg & impact of crop management - Fapesp
bnf in sugarcane, ghg & impact of crop management - Fapesp
bnf in sugarcane, ghg & impact of crop management - Fapesp
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CO-CULTURES AS A MODEL TO STUDY INTERACTIONS BETWEEN<br />
SUGARCANE CALUSES AND DIAZOTROPHIC ENDOPHYFITIC<br />
BACTERIA.<br />
R. C. R. MARTINS AND H. R. BARBOSA<br />
Callus<br />
Co-culture<br />
8<br />
• Influence <strong>of</strong> callus on bacterial growth – STIMULATION x INHIBITION<br />
Callus<br />
log CFU.mL -1<br />
10<br />
9<br />
8<br />
7<br />
6<br />
5<br />
4<br />
3<br />
2<br />
1<br />
Enterobacter sp. (ICB117)<br />
10<br />
9<br />
8<br />
Erw<strong>in</strong>ia sp. (ICB409)<br />
0<br />
0 200 400 600 800 1000 1200<br />
time (h)<br />
log CFU.mL -1<br />
log CFU.mL -1<br />
7<br />
6<br />
5<br />
4<br />
3<br />
2<br />
1<br />
0<br />
0 200 400 600<br />
time (h)<br />
• Influence <strong>of</strong> bacteria on calluses– INCREASE OF PROTEIN<br />
CONTENT-through N-fixation<br />
Culture medium<br />
N comb<strong>in</strong>ed-free<br />
Bioen Workshop 2012 (Cantarella)<br />
prote<strong>in</strong> content <strong>of</strong> calluses<br />
600<br />
500<br />
400<br />
300<br />
200<br />
100<br />
Callus + Erw<strong>in</strong>ia sp.<br />
0<br />
0 200 400 600 800 1000 1200 1400<br />
Time (h)<br />
Callus +<br />
Enterobacter sp.<br />
Callus control
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