Dr Harbans Bariana, University of Sydney - Australian Centre for ...
Dr Harbans Bariana, University of Sydney - Australian Centre for ...
Dr Harbans Bariana, University of Sydney - Australian Centre for ...
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MAS FOR BIOTIC STRESSES<br />
<strong>Harbans</strong> <strong>Bariana</strong>,<br />
Urmil Bansal, Rohit Mago, Wolfgang Spielmeyer, Haydn Kuchel, Mathew Hayden,<br />
Evans Lagudah, Robert Park<br />
GRDC<br />
Grains<br />
Research &<br />
Development<br />
Corporation<br />
Plant Breeding Institute Cobbitty<br />
<strong>Australian</strong> Cereal Rust Control Program<br />
<strong>Australian</strong> Winter Molecular Marker Program<br />
Faculty <strong>of</strong> Agriculture, Food & Natural Resources<br />
© <strong>University</strong> <strong>of</strong> <strong>Sydney</strong>
Breeding For Disease Resistance<br />
HOST RESEARCH PATHOGEN<br />
RESEARCH<br />
GERMPLASM ENHANCEMENT and SCREENING and MARKER<br />
DEVELOPMENT and VALIDATION<br />
BREEDERS/OTHERS<br />
GROWERS<br />
Faculty <strong>of</strong> Agriculture, Food & Natural Resources
Molecular Biologists<br />
Administrators<br />
Physiologists<br />
Breeding – A complex game<br />
Cereal Chemists<br />
Breeder<br />
Other disciplines<br />
Pathologists<br />
Farmers<br />
Agronomists<br />
Faculty <strong>of</strong> Agriculture, Food & Natural Resources<br />
© <strong>University</strong> <strong>of</strong> <strong>Sydney</strong>
<strong>Australian</strong> Winter Cereal Molecular Program<br />
• Enabling technologies<br />
• Genetic analysis<br />
• Simple protocols<br />
• Validation and Implementation<br />
Faculty <strong>of</strong> Agriculture, Food & Natural Resources<br />
© <strong>University</strong> <strong>of</strong> <strong>Sydney</strong>
Faculty <strong>of</strong> Agriculture, Food & Natural Resources<br />
7DS<br />
5BL<br />
LR/SR<br />
© <strong>University</strong> <strong>of</strong> <strong>Sydney</strong>
Impact <strong>of</strong> marker research on implementation<br />
500 assays<br />
>$50/data point<br />
RFLPs<br />
1997<br />
Low throughput DNA extraction<br />
50,000+ assays<br />
Implementation <strong>of</strong> MAS in <strong>Australian</strong> Wheat Breeding<br />
• Enrichment <strong>of</strong> desirable alleles – 60 -75%<br />
• MAS backcrossing – upto 10%<br />
• Gene pyramiding – upto 25%<br />
• Fixed line/Crossing block parent characterisation –10-25%<br />
Faculty <strong>of</strong> Agriculture, Food & Natural Resources<br />
© <strong>University</strong> <strong>of</strong> <strong>Sydney</strong>
Increasing gene frequency in early generation populations<br />
Faculty <strong>of</strong> Agriculture, Food & Natural Resources
New/Recycled Genes<br />
• Sr46 – new gene with linked marker<br />
• QSr-Sun-5BL (Arina) – APR fro stem rust<br />
• QSr-Sun-7DS (Forno) – Lr34 linked<br />
• Yr4 in spring wheat germplasm including<br />
CIMMYT and ICARDA material marker available<br />
• Lr48 flanking markers currently being<br />
validated<br />
R R R S S<br />
• APR to all three rust diseases in Watkin’s collection and<br />
more<br />
Faculty <strong>of</strong> Agriculture, Food & Natural Resources<br />
© <strong>University</strong> <strong>of</strong> <strong>Sydney</strong>
Available Markers<br />
• Sr2, Sr22, Sr24, Sr25, Sr26, Sr32, Sr36, Sr39, Sr40, SrR,<br />
Sr46, QSr-Sun-7D (Lr34 from Forno)<br />
• Lr19, Lr21, Lr25, Lr29, Lr34, Lr46?, Lr47, Lr48 (Loosely<br />
linked flanking markers<br />
• Yr4, Yr10, Yr15/Yr24, Yr17, Yr18, Yr29?, Yr36<br />
• Black point resistance 2B, CCN genes, Glu-1 and Glu-3<br />
genes<br />
Faculty <strong>of</strong> Agriculture, Food & Natural Resources<br />
© <strong>University</strong> <strong>of</strong> <strong>Sydney</strong>
Key Messages<br />
• MAS requires well designed crosses based on the<br />
knowledge <strong>of</strong> trait marker relationship among the parents<br />
• Priorities <strong>for</strong> target marker will vary based on parental<br />
selection, financial and human resources<br />
• There is no fixed way <strong>for</strong> MAS, individual creativity is<br />
essential <strong>for</strong> success<br />
• Combination <strong>of</strong> MAS and phenotypic selection based on a<br />
carefully thought strategy will assure positive outcome<br />
• Effective multidisciplinary communication<br />
Faculty <strong>of</strong> Agriculture, Food & Natural Resources<br />
© <strong>University</strong> <strong>of</strong> <strong>Sydney</strong>
Thank You<br />
Faculty <strong>of</strong> Agriculture, Food & Natural Resources<br />
© <strong>University</strong> <strong>of</strong> <strong>Sydney</strong>
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