Plant Alkaloids Atallah F. Ahmed
Plant Alkaloids Atallah F. Ahmed
Plant Alkaloids Atallah F. Ahmed
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Tests of Identity<br />
Mostly depend on the presence of indole nucleus.<br />
1. Van Urk test: Ergot alkaloids solution + Van Urk<br />
reagent (p-dimethyl-aminobenzaldehyde + 5% FeCl 3 +<br />
15% sulfuric acid) deep blue color.<br />
The Van Urk reagent can be used as spray reagent for<br />
PC or TLC of the ergot alkaloids (10% HCl is uesd in<br />
place of sulfuric acid). The spectrophtometric assay<br />
method for ergot alkaloids is based on this blue color.<br />
2. Keller's test: Ergot alkaloids solution in acetic acid +<br />
trace of FeCl3 + conc. sulfuric acid carefully added <br />
intense blue colored band at the boundary of two layers layers.<br />
3. Glyoxylic (formylformic) acid reagent + conc. sulfuric<br />
acid + ergot alkaloids blue color.<br />
4. The aqueous salts solution blue flourescence.<br />
<strong>Atallah</strong> F. <strong>Ahmed</strong>, PhD 2010<br />
Pharmacological action and uses<br />
Mechanism: Ergot alkaloids act at α-adrenergic,<br />
dopaminergic and serotonergic receptors. The<br />
relationship of the alkaloid structure to those of<br />
noradrenaline, dopamine, and and5-hydroxytryptamine 5 hydroxytryptamine (5- (5<br />
HT, serotonin) is shown:<br />
<strong>Atallah</strong> F. <strong>Ahmed</strong>, PhD 2010<br />
27<br />
28<br />
5/30/2010<br />
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