Supporting Online Material for - Science

www.sciencemag.org/cgi/content/full/317/5837/523/DC1 

Supporting Online Material for 

Candidatus Chloracidobacterium thermophilum: An Aerobic Phototrophic 

Acidobacterium 

Donald A. Bryant, * Amaya M. Garcia Costas, Julia A. Maresca, Aline Gomez Maqueo 

Chew, Christian G. Klatt, Mary M. Bateson, Luke J. Tallon, Jessica Hostetler, William C. 

Nelson, John F. Heidelberg, David M. Ward 

*To whom correspondence should be addressed. E-mail: dab14@psu.edu 

This PDF file includes: 

Materials and Methods 

SOM Text 

Figs. S1 to S13 

Table S1 

References 

Published 27 July 2007, Science 317, 523 (2007) 

DOI: 10.1126/science.1143236

Supporting Online Material for 

A phototrophic acidobacterium discovered by metagenomics 

D. A. Bryant*, A. M. Garcia Costas, J. A. Maresca, A. Gomez Maqueo Chew, C. Klatt, M. M. 

Bateson, L. J. Tallon, J. Hostetler, W. C. Nelson, J. F. Heidelberg, and D. M. Ward 

*To whom correspondence should be addressed. E-mail: dab14@psu.edu 

This supplement contains: 

Materials and Methods 

SOM text 

Figs. S1 to S13 

Table S1 

References 

1

Supporting Online Material 

MATERIALS AND METHODS 

Library construction, DNA sequencing, and sequence analysis 

The sequence of a representative Roseiflexus sp. isolate, strain RS1 (1-3) was determined 

as part of a Joint Genome Institute-Department of Energy project to determine genome 

sequences of filamentous anoxygenic phototrophs (http://genome.jgi-psf.org/; GenBank 

accession AAQU00000000). The Synechococcus sp. OS-A and OS-B' genome sequences (4) 

(GenBank accessions CP000239 and CP000240) and metagenomic clone libraries and databases 

(4-6) used in this study were developed for an NSF Frontiers in Integrative Biology Research 

project (7). Amplicon Express (Pullman, WA) constructed the bacterial artificial chromosome 

(BAC) clone library. Sequence data derived from BAC clone M60-018 J19 (see Fig. 6S) have 

been deposited in GenBank under accession number EF531339. 

Sequencing and assembly of the metagenome were performed as described for genomes 

sequenced by TIGR and the J. Craig Venter Institute (8). BAC closure was done by mechanical 

shearing of the entire BAC followed by random shotgun sequencing on small insert (2-3 kbp) 

clones. The plasmid sequences were jointly assembled using the TIGR Assembler (9). The 

coverage criteria were that every position required at least double-clone coverage (or sequence 

from a PCR product amplified from genomic DNA), and either sequence from both strands or 

with two different sequencing chemistries. The sequence was edited manually, and additional 

PCR (10) and sequencing reactions were performed to close gaps, improve coverage, and resolve 

sequence ambiguities. 

2

An initial set of open reading frames likely to encode proteins (coding sequence, CDS) 

were predicted for the BAC insert by Glimmer (11). All predicted proteins larger than 30 amino 

acids were searched against a non-redundant protein database as previously described (12-14). 

TopPred was used to predict the membrane-spanning domains of proteins (15). The 5' regions of 

each CDS were inspected to define initiation codons using homologies, and position of 

ribosomal binding sites and transcriptional terminators. Two sets of hidden Markov models were 

used to determine CDS membership in families and superfamilies: pfam v11.0 (16) and 

TIGRFAMs 3.0 (17). Pfam v11.0 hidden Markov models were also used with a constraint of a 

minimum of two hits to find and mask repeated domains within proteins. 

Database searches were performed using the various blast algorithms to search the 

GenBank non-redundant databases (18, 19). For construction of phylogenetic trees, protein 

sequences were aligned using the ClustalW tool in MacVector versions 7.2.3 or 9.0.2 (Accelrys, 

San Diego, CA). Neighbor-joining trees for type-1 reaction centers and BchU were generated 

from the alignments using PAUP 4.0 (Sinauer Associates, Inc., Sunderland, MA). The neighbor- 

joining tree for RecA was generated using MacVector version 9.0.2 (Accelrys, San Diego, CA). 

Enrichment culture methods and isolation of Anoxybacillus sp. 

Enrichment cultures containing Synechococcus sp. strain JA-2-3B'a (2-13), 

Chloracidobacterium (Cab.) thermophilum, and Anoxybacillus sp. (20) were grown in B-HEPES 

medium at pH 8.0 (21) at 45-53°C. In cultures to maintain and enrich for Cab. thermophilum, the 

B-HEPES medium was supplemented with 1 mM ammonium chloride and a mixture of carbon 

sources: lactate, succinate, glycolate, butyrate, propionate, and acetate (1 mM each, added in the 

form of sodium salts). Nitrate was omitted from the growth medium of cultures that did not 

contain cyanobacteria. The medium was also supplemented with filter-sterilized vitamins at the 

3

following final concentrations: (10 µg L -1 ) riboflavin, vitamin B12 (100 µg L -1 ), thiamine-HCl 

(100 µg L -1 ), L-ascorbic acid (100 µg L -1 ), D-Ca-panthothenate (100 µg L -1 ), folic acid (100 µg 

L -1 ), nicotinic acid (100 µg L -1 ), 4-aminobenzoic acid (100 µg L -1 ), pyridoxine-HCl (100 µg L -1 ), 

and lipoic acid (100 µg L -1 ). To inhibit the growth of the cyanobacterial cells in the original 

enrichment, atrazine was added to a final concentration of 10 µM. 

Unless otherwise specified, all cultures were incubated under oxic conditions with or 

without shaking in constant light provided by a 60 W tungsten and a 60 W fluorescent bulb (total 

visible light intensity of 67 µmol photons m -2 s -1 ). Synechococcus sp. strain JA-2-3B'a (2-13) was 

eliminated from an enrichment that also contained Anoxybacillus sp. and Cab. thermophilum by 

serial subculturing three times in the light in the presence of 10 µM atrazine. Anoxybacillus sp. 

was isolated in axenic culture by plating cells from the enrichment cultures on Luria-Bertani 

medium solidified with 1.5% (w/v) Difco Bacto-Agar (Becton-Dickinson, Franklin Lakes, NJ). 

Anoxybacillus sp. was identified by amplifying and DNA sequencing a region of its 16S rRNA 

by using universal bacterial primers (Table 1S). 

Light microscopy 

Cab. thermophilum cells were incubated in 1-hexanol-saturated phosphate-buffered 

saline to disrupt bacteriochlorophyll (BChl) c aggregates (22) and visualized in an Olympus BX- 

60 epifluorescence upright microscope equipped with an UplandFL 100× oil objective, a 

mercury vapor lamp and Olympus filter cubes 41001 (Olympus, Center Valley, PA). 

Absorption and fluorescence emission spectra 

4

Absorption spectra were either recorded with a Cary 14 UV-Vis-NIR spectrophotometer 

modified for computerized data acquistion by On-Line Systems, Inc. (Bogart, GA) or with a 

GENESYS 10 spectrophotometer (Thermo Electron Corp., Rochester, NY). Data were analyzed 

using Igor-Pro software (Wavemetrics, Inc. Lake Oswego, OR). 

Chlorosome isolation and electron microscopy 

Chlorosomes were isolated from fresh cells (2 L) as described by Vassilieva et al. (23). 

Isolated chlorosomes were negatively stained with 1% (w/v) uranyl acetate and visualized in a 

JEOL 1200 EXII Transmission electron microscope (Peabody , MA). 

Pigment extraction and analysis 

Pigments were extracted from 1-ml aliquots of cell suspension from late-exponential 

phase enrichment cultures by sonication in acetone-methanol (7:2, v/v). Cell debris was removed 

by centrifugation. The absorption spectrum of the pigment mixture was recorded after diluting 

the sample at least 1:10 in methanol, and the absorption spectrum of the extracts was recorded 

from 350 to 900 nm. Samples for HPLC analysis were filtered and 0.1 volume of 1 M 

ammonium acetate was added to the filtrate immediately before injection into the HPLC column. 

The pigments were separated on a 25 cm by 4.6 mm Discovery 5 µm C18 column (Supelco, 

Bellefonte, PA) attached to a 1,024-element diode array detector (model G1315B, 1100 series; 

Agilent Technologies, Palo Alto, CA) controlled with Agilent ChemStation software for HPLC. 

The solvent system was previously described (24). BChl c and BChl a extracted from Chl. 

tepidum and from Cfx. aurantiacus Y-400-fl served as standards (see Fig. 4). Elution of the 

pigments was monitored at 667 and 770 nm, the absorbance maxima for BChl c and BChl a 

5

espectively; the absorption spectrum of the column eluate was recorded from 200 to 900 nm at 

two-second intervals. 

Polymerase chain reaction and agarose gel electrophoresis 

In general, the polymerase chain reaction conditions consisted of 5 minutes at 95 o C, 35 

cycles of 45 seconds at 95 o C, 45 seconds at 57 o C and 1 minute at 72 o C, followed by a final 

elongation step of 10 minutes at 72 o C. About 10-100 ng of DNA were included in each reaction 

and primers (see Table 1S) were supplied at a final concentration of 1 µM. PCR products were 

electrophoresed in a 0.8% (w/v) agarose gel and stained with ethidium bromide prior to 

visualization. 

SUPPORTING ON-LINE MATERIAL 

History of discovery of photosynthetic bacteria and their differentiating characteristics 

Five bacterial phyla (Cyanobacteria, Chlorobi, Proteobacteria, Chloroflexi, and 

Firmicutes) contain members, chlorophototrophs, capable of phototrophy based upon BChl or 

chlorophyll (Chl) (25). (Throughout this article, we have used the terms phylum and phyla as 

employed in the Second Edition of Bergey’s Manual of Systematic Bacteriology (26), although 

there is debate as to whether the major sublineages of Domains should be considered kingdoms, 

as in the case of plants, animals, and fungi.). Studies of Cyanobacteria and photosynthetic 

Proteobacteria (i.e. the purple bacteria) date to the first half of the 19 th century, and green sulfur 

bacteria (Chlorobi) were first described in 1906 (26, 27). Cyanobacteria perform oxygenic 

photosynthesis by virtue of their Chl a-containing, type-1 (photosystem I) and type-2 

(photosystem II) reaction centers, and they fix carbon by the reductive pentose-phosphate 

6

(Calvin-Benson-Bassham) cycle. Endosymbiosis of a cyanobacterium led to the acquisition of 

oxygenic photosynthesis by eukaryotic algae and plants. The purple chlorophototrophic bacteria, 

all members of the alpha, beta, and gamma subdivisions of the Proteobacteria, have relatively 

simple, type-2 reaction centers with either BChl a or BChl b, do not evolve oxygen, and also fix 

carbon via the reductive pentose phosphate pathway, although most can grow mixotrophically 

when presented with light and a usable organic carbon source. Many purple bacteria are aerobic 

chemoheterotrophs under oxic conditions, but are only facultatively phototrophic under anoxic 

conditions. Chlorobi are strictly anaerobic, photoautotrophic organisms that have type-1 reaction 

centers with chlorosomes as antennae; any given strain can produce three types of Chl (Chl a, 

BChl a, and either BChl c, d, or e) and fix CO2 by the reverse TCA cycle. 

In the 100 years since the initial description of the Chlorobi (27), only two additional 

bacterial phyla have been shown to contain members that can produce BChls and reaction 

centers. Pierson and Castenholz (28) isolated the filamentous anoxygenic phototroph, 

Chloroflexus aurantiacus, from the same microbial mats of Octopus Spring and other thermal 

features around the world. This moderate thermophile synthesizes BChls a and c, produces 

chlorosomes as light-harvesting antennae, has type-2 reaction centers similar to those of purple 

bacteria, and fixes CO2 by the 3-hydroxypropionate cycle. Cfx. aurantiacus is the type species of 

the Chloroflexi, an early-diverging phylum of the Bacteria. Strains of both major subdivisions of 

the Chloroflexi contain the genes required for carbon fixation by the 3-hydroxypropionate cycle 

(2), although it should be noted that some chlorophototrophic Chloroflexi possess Rubisco and 

fix carbon by the reductive pentose-phosphate pathway (29). The most recently discovered 

reaction center-containing bacteria are the heliobacteria, which were originally described by Gest 

and Favinger in 1983 (30). Heliobacteria are Gram-positive bacteria, i.e., Firmicutes, which form 

7

heat-resistant endospores and that are closely related to clostridia (see Fig. 8S). These strict 

anaerobes produce type-1 reaction centers containing BChl g, but no known isolate can grow 

photoautotrophically. 

Sequence analysis of a pscAB-fmoA operon recovered from the metagenome of the 

phototrophic mat community of alkaline siliceous hot springs 

Inserts encoding the highly divergent pscA gene of an organism from the phototrophic 

layer of the microbial mats of Octopus and Mushroom Springs in Yellowstone NP were 

completely sequenced from four independent plasmids and one BAC insert (see below; GenBank 

accession EF531339). The overlapping data revealed a probable operon, 5’pscAB-fmoA, 

encoding the type-1 reaction center (PscAB) and the FmoA apoprotein of the BChl a-binding 

Fenna-Matthews-Olson protein (Fig. 1B, Fig. 6S). Preliminary phylogenetic analyses of the 

flanking genes suggested that these DNA sequences were derived from an organism related to 

Acidobacterium sp. Ellin345 or Solibacter usitatus Ellin6076, two soil bacteria belonging to the 

poorly characterized phylum Acidobacteria (26), whose genomes have recently been sequenced 

(3). 

The three sequences deduced from the pscAB-fmoA gene cluster were divergent from 

known homologs in other anoxygenic chlorophototrophs. PscA was predicted to be a polypeptide 

of 865 amino acids (aa), which was ~135 aa larger than the PscA subunits of green sulfur 

bacterial type-1 reaction centers, to which it was most similar (31) (Fig. 1A, 1S). This reaction 

center apoprotein was more distantly related to PshA (~610 aa) of heliobacteria and the PsaA 

and PsaB subunits (730-750 aa) of cyanobacterial photosystem I (Figs. 1, 1S-3S). Like the 

apoproteins of all other type-1 reaction centers, the mat PscA was predicted to contain eleven, 

8

transmembrane α-helices. An insertion of ~165 aa between predicted transmembrane α-helices 

VII and VIII accounted for most of the size difference (Fig. 1B, 1S). This insertion corresponded 

to a large extrinsic loop, which might form a docking site for a periplasmic electron donor to the 

oxidized special pair. The C-terminal electron transfer domain of PscA, comprising the last four 

transmembrane α-helices of the protein, was most similar to the same domain of green sulfur 

bacterial PscA sequences (Figs. 1, 3S), but this domain was nevertheless quite divergent (48% 

identical, 69% similar). However, the highly conserved motif, FPCIGPVKGGTCGVS, 

containing two cysteine residues in the cytoplasmic loop between transmembrane helices VIII 

and IX (Fig. 3S), implied that this reaction center contains an intersubunit [4Fe-4S] cluster 

similar to FX of PS I (31). The N-terminal, antenna domain of this PscA-like protein is not well 

conserved and was difficult to align to other reaction center sequences (Fig. 2S). It exhibits only 

~29% identity and ~43% similarity to the N-terminal antenna domain of PscA from green sulfur 

bacteria. 

Similar to PscB of green sulfur bacterial Type-1 reaction centers (31) and the PsaC 

protein of photosystem I (32, 33), the mat-derived PscB was predicted to be a small protein with 

eight conserved cysteines, which should ligate two terminal, electron-accepting, [4Fe-4S] 

clusters (see Fig. 4S). The region encompassing these cysteines was well conserved, but the 

remainder of the protein was much less similar to PscB sequences of green sulfur bacteria. The 

fmoA gene encodes the Fenna-Matthews-Olson protein, a BChl a-binding protein that occurs in 

all green sulfur bacteria (34). The fmoA gene is only known to occur in green sulfur bacteria, but 

in 12 sequenced green sulfur bacterial strains this gene is unlinked to pscAB operons. Although 

the FmoA proteins of green sulfur bacteria are highly similar in sequence (~70% identity, ~85% 

similarity), the pscAB-linked fmoA gene encoded a protein that is only ~40% identical and 58% 

9

similar to FmoA sequences of green sulfur bacteria (Fig. 5S). Based upon these data, we 

hypothesized that a thermophilic acidobacterium with a novel type-1 reaction center and FmoA 

occurs in the mats. 

Identification and sequencing of a bacterial artificial chromosome insert encoding pscAB- 

fmoA, recA, and the rDNA operon of an acidobacterium 

To compile more information about the putative acidobacterium with type-1 reaction 

centers, metagenomic reads from ~60°C and ~65°C sites of the Octopus and Mushroom Spring 

microbial mats were assembled to produce consensus contigs. Most of the biomass in these mats 

belongs to organisms closely related to Synechococcus sp. OS-A and OS-B' and Roseiflexus sp. 

RS1 (35, 36), whose genomes have been sequenced (2-4, 37). Sequences showing >92% identity 

over 80% of the read to these three organisms were first removed. Contigs with G+C contents of 

~56 mol% and with multiple gene products showing best hits from blastx analyses to 

acidobacteria and/or δ-proteobacteria were then binned as possibly being derived from a single 

type of mat bacterium. This bin contained two scaffolds that encoded well-established 

phylogenetic markers: a complete recA gene and a partial 16S rRNA gene. Bacterial artificial 

chromosome (BAC) clones, constructed from DNA isolated from the photic-zone mat layer of 

the ~60°C Mushroom Spring site, were end-sequenced and mapped onto the binned scaffolds 

(Fig. 6S). These analyses predicted that the binned pscAB-fmoA, recA, and the rRNA genes were 

encoded on a single BAC insert. Sequencing of this 271,846 bp BAC insert (Fig. 6S) confirmed 

that the pscAB-fmoA and rRNA operons were separated by ~183 kbp with the recA gene between 

them. The complete 16S rRNA sequence derived from the BAC clone is identical to the partial 

sequence assembled from the metagenome and is nearly identical (differences at 2 of 1348 

10

shared positions) to that from an acidobacterium, denoted GFP1, the 16S rRNA sequence of 

which was recovered from Green Finger Pool in the Lower Geyser Basin of Yellowstone NP (a 

site ~5 km from Octopus Spring) (see Fig. 1 of 38) (Fig. 7S). These data unequivocally establish 

that the highly divergent pscAB-fmoA genes are derived from a GFP1-like organism belonging to 

the phylum Acidobacteria. Phylogenetic analyses of RecA are consistent with this conclusion 

(Fig. 8S, 9S). 

Analyses of sequences tentatively assigned to the GFP1-like acidobacterium. 

The binned sequence assemblies putatively derived from the GFP1-like acidobacterium 

were annotated by computer and analyzed. The acidobacterial sequence bin contained several 

genes for BChl and carotenoid biosynthesis as well as genes associated with chlorosomes. For 

example, bchU (Fig. 10S, 11S), whose product is a C-20 methyltransferase required for the 

synthesis of BChl c of green bacteria (39) and bchK, whose product is the BChl c synthase (40), 

were found. A radical-SAM-type methyltransferase similar to BchR, which methylates C-12 1 of 

BChl c (41), was also identified. These data predicted that the acidobacterium should produce 

methylated Bchl c homologs and assemble chlorosomes like green sulfur bacteria. Consistent 

with this inference, a csmA gene, whose product is a BChl a-binding protein of the chlorosome 

baseplate (42), was also found in a putative operon with bchG, encoding a BChl a synthase (see 

Fig. 6S). Overlapping metagenome scaffolds and BAC clones physically linked these genes and 

bchY, a subunit of chlorophyllide a reductase, to the genes on the sequenced BAC insert (Fig. 

6S). No oxygen-independent magnesium-protoporphyrin IX monomethyl ester oxidative cyclase 

(bchE) was identified. However, the presence of acsF, encoding an oxygen-dependent 

magnesium-protoporphyrin IX monomethyl ester oxidative cyclase (41, 43, 44), implied that the 

11

mat acidobacterium should synthesize BChl under oxic conditions (Fig. 12S). Genes with strong 

sequence similarity to crtB, crtP, and crtH further imply that the GFP1-like organism synthesizes 

carotenoids by a pathway similar to that in Chlorobi and Cyanobacteria (45). Although an mcr 

gene encoding malonyl-CoA reductase, an enzyme required for CO2 fixation by the 3- 

hydroxypropionate pathway (2), was identified, no other genes specifically attributable to CO2 

fixation were identified. No genes encoding hydrogenases or enzymes for sulfide/sulfur 

oxidation were detected. 

12

Supplemental Figure Legends 

Figure 1S. Diagram comparing the relative positions of transmembrane α-helices and loops of 

various Type-1 reaction center polypeptides. Bars connecting the tops of the rectangles represent 

loops in the cytoplasm and bars connecting the bottoms of the rectangles represent loops in the 

periplasm or thylakoid lumen. Note the very large periplasmic loop between helices VII and VIII 

of the Cab. thermophilum. The positions of the FX Cys ligands and the conserved histidine ligand 

to the reaction center special pair (P700, P840, P780) are indicated. The antenna (helices I to VI) 

and electron-transfer domains (helices VII to XI) of the polypeptides are indicated. 

Abbreviations and sequence alignments of selected Type-1 reaction center proteins can be found 

in Figures 2S and 3S. 

Figure 2S. Sequence alignment of the N-terminal region of PscA of Cab. thermophilum and the 

homologous PscA, PshA, PsaA, and PsaB proteins of the Type-1 reaction centers and other 

photosynthetic bacteria. The region shown corresponds to that including transmembrane α– 

helices I through VII, including all of the antenna domain and the first transmembrane helix of 

the electron transfer domain; the positions of these helices for Thermosynechococcus elongatus, 

as derived from the X-ray structure for photosystem I (47), are indicated. Note that the long 

inserted loop sequence that follows helix VII of Cab. thermophilum has been truncated, since it 

is not related in sequence to any portion of any other reaction center protein. Hyphens indicate 

insertions/deletions included to optimize the alignment. Abbreviations: Cab., 

Chloracidobacterium; Chl., Chlorobium; Hbc., Heliobacillus; Hbt., Heliobacterium; Pld., 

Pelodictyon; Ptc., Prosthecochloris; T., Thermosynechococcus. 

13

Figure 3S. Sequence alignment of the C-terminal region of PscA and the homologous PshA, 

PsaA, and PsaB proteins of the reaction centers of Cab. thermophilum and other photosynthetic 

bacteria. The region shown corresponds to transmembrane α–helices VIII through XI, including 

most of the electron-transfer cofactor binding domain; the positions of these helices for 

Thermosynechococcus elongatus as derived from the X-ray structure for photosystem I (47) are 

shown. Hyphens indicate insertions/deletions included to optimize the alignment. The position of 

the conserved, cysteine-containing loop (red boxes enclose the conserved cysteine residues) that 

is predicted to provide the ligands to an intersubunit FX-like, [4Fe-4S] cluster is indicated by the 

bar labeled FX. The conserved His in Helix X that is the putative ligand to one BChl/Chl of the 

reaction center special pair is also indicated by a red box. Abbreviations are the same as in 

Figure 3S. 

Figure 4S. Sequence alignment of PscB sequences from various organisms. Hyphens indicate 

insertions/deletions included to optimize the alignment. Abbreviations: Cab., 

Chloracidobacterium; Chl., Chlorobium; Ptc., Prosthecochloris. The eight conserved cysteines 

that are likely to be the ligands to two [4Fe-4S] clusters are indicated in red boxes and in the 

consensus sequence. 

Figure 5S. Sequence alignment of selected FmoA proteins, the apoprotein of the BChl a-binding 

Fenna-Matthews-Olson protein. Hyphens indicate insertions/deletions included to optimize the 

alignment. Abbreviations: Cab., Chloracidobacterium; Chl., Chlorobium; and Ptc., 

Prosthecochloris. 

14

Figure 6S. Physical map of a 950-kbp region of the consensus Cab. thermophilum genome, 

including BAC clone M60-018 J19 and showing the relative positions of the rRNA operon, recA, 

pscA-pscB-fmoA, bchG-orf-csmA, and bchY genes. A. Physical map of assembled scaffolds and 

contigs from the Mushroom Spring metagenome (dark blue bars) mapped onto the sequence of 

BAC clone M60-018 J19 (blue-green bar). This BAC clone is completely sequenced and 

comprises 271,846 bp (GenBank accession EF531339). The colored boxes indicate the positions 

of the 16S rRNA (purple), recA (red), and pscAB-fmoA (green) genes. B. Extension of the map in 

Panel A that shows the physical map of sequenced BAC clone M60-018 J19 (blue-green bar) and 

other BAC clones (gray bars) whose end-sequences (pink boxes) were mapped onto the scaffolds 

and contigs (dark blue bars) assembled from the Mushroom and Octopus Springs metagenome. 

The positions of the bchG-orf-csmA (yellow-green) and bchY (orange) genes are indicated, 

establishing that these genes are also physically linked to the 16S rRNA gene in BAC clone 

M60-018-J19. 

Figure 7S. Comparison of the complete sequence of the 16S rRNA of Cab. thermophilum 

derived from BAC clone M60-018 J19 with the nearly complete sequence from Acidobacterium 

GFP1 (37). The red ellipses indicate the differences between the two sequences. 

Figure 8S. Rooted neighbor-joining phylogram based upon the sequence alignment shown in 

Figure 9S, which shows the relationships of RecA sequences of Cab. thermophilum and various 

other organisms. The RadA-like protein (DR1105) of Deinococcus radiodurans was used as the 

outgroup sequence. Some of the major eubacterial phyla are listed at the right. 

Chlorophototrophs and the phyla that contain them are shown in colors, and all other phyla and 

15

organisms are indicated in black. Bootstrap values were determined on the basis of 2000 

resamplings. 

Figure 9S. Alignment of the RecA sequences from the indicated organisms that was used to 

generate the tree shown in Figure 8S. Hypens indicate insertions/delections included to optimize 

the alignment. Abbreviations: Cab., Chloracidobacterium; Car., Carboxydothermus; Cfx., 

Chloroflexus; Hb., Heliobacterium; Pel., Pelotomaculum; Pld., Pelodictyon; The., 

Thermoanaerobacter. 

Figure 10S. Rooted neighbor-joining phylogram derived from the sequence alignment shown in 

Figure 11S showing the relationship of the BchU sequence of Cab. thermophilum to the BchU 

and CrtF sequences of various organisms. The ComT methyltransferase of Ara. thaliana was 

used as the outgroup. Abbreviations: A., Arabidopsis; Cab., Chloracidobacterium; Cfx., 

Chloroflexus; Chl., Chlorobium; Pld., Pelodictyon; Ptc., Prosthecochloris; Rhb., Rhodobacter; 

Rps., Rhodopseudomonas; Rvx., Rubrivivax. The bootstrap values shown are based on 1000 

resamplings. 

Figure 11S. Alignment of BchU, CrtF, and ComT sequences from the indicated organisms that 

was used to generate the tree shown in Figure 10S. Hyphens indicate insertions/deletions 

included to optimize the alignment. Abbreviations: A., Arabidopsis; Cab., Chloroacidobacterium; 

Cfx., Chloroflexus; Chl., Chlorobium; Pld., Pelodictyon; Ptc., Prosthecochloris; Rhb., 

Rhodobacter; Rps., Rhodopseudomonas; Rvx., Rubrivivax. 

16

Figure 12S. Alignment of sequences for AcsF, the oxygen-dependent oxidative ring cyclase for 

Mg-protoporphyrin IX monomethyl ester in BChl and Chl biosynthesis (41, 43, 44). Hyphens 

indicate insertions/deletions included to optimize the alignment. Abbreviations: A., Arabidopsis; 

Cab., Chloracidobacterium; Cfx., Chloroflexus; G., Gloeobacter; Por., Porphyra; Rb., 

Rhodobacter; Rfx., Roseiflexus; Rps., Rhodopseudomonas. 

Figure 13S. Complete HPLC elution profile monitored at 667 nm for pigments extracted from 

an enrichment culture containing Synechococcus sp. strain JA-2-3B'a (2-13), Cab. thermophilum, 

and Anoxybacillus sp. The blue-colored block indicates the elution positions of BChl c homologs 

esterified with farnesyl (indicated by the subscript “F”). The yellow colored block indicates the 

elution position of BChl c homologs esterified with geranylgeraniol (indicated by the subscript 

“GG”). Most of the BChl c is esterified with alcohols more hydrophobic than geranylgeraniol 

(aqua colored block), and at least two distinct alcohol groups appear to be present because of the 

complexity of the pattern observed. The peaks numbered 1 to 4 indicate the typical pattern of 

[ethyl, methyl], [ethyl, ethyl], [propyl, ethyl] and [i-butyl, ethyl] BChl c homologs, and the 

letters “a” and “b” indicate peaks probably derived from two different esterifying alcohol groups. 

Peaks from Chl a of Synechococcus sp. strain JA-2-3B'a (2-13) are indicated by the two arrows. 

The inset shows the spectrum of peak 3b, which is characteristic of BChl c under these solvent 

conditions. 

17

Figure 1S 

Antenna domain 

Synechococcus sp. 7002 PsaA 

Synechococcus sp. 7002 PsaB 

Chl. tepidum PscA 

Hbt. modesticaldum PshA 

Cab. thermophilum PscA 

Electron transfer domain 

F X 

F X 

F X 

F X 

F X 

P840 

= Transmembrane alpha-helix 

P700 

P780 

P700 

P840 

739 aa 

733 aa 

731 aa 

608 aa 

865 aa

PscA/PshA/PsaA/PsaB/ N-terminal Alignments 

T. elongatus PsaB 

Synechococcus 7002 PsaB 

T. elongatus PsaA 

Synechococcus 7002 PsaA 

Hbc. mobilis PshA 

Hbt. gestii PshA 



Ptc. aestuarii PscA 

Pld. luteolum PscA 


Chl. phaeobacteroides 266 PscA 

Chl. phaeo. PscA 

Chl. limicola PscA 

Chl. chlorochromatii PscA 

Chl. vibrioforme PscA 

Chl. phaeo BS1 PscA 

Chl. limicola thio. PscA 

10 20 30 40 50 60 70 80 90 100 110 120 

M A T K F P K F S Q D L A Q D P - T T R R I W Y A I A M A H D F E S H D G M T E E N L Y Q K I F A S H F G H L A I I F L W V S G S L F H V A W Q G N F E Q W V Q 

M A T K F P K F S Q D L A Q D P - T T R R I W Y G I A T A H D F E T H D G M T E E N L Y Q K I F A S H F G H L A I I F L W T S G T L F H V A W Q G N F E Q W I K 

M P S F L P C L R E R R D S M T I S P P E R E P K V R V V V D N D P V P T S F E K W A K P G H F D R T L A R G P Q T T T W I W N L H A L A H D F D T H T - S D L E D I S R K I F S A H F G H L A V V F I W L S G M Y F H G A K F S N Y E A W L A 

M A V K V D K D P V P T S F E K W G K P G H F D R T L A R G P K T T T W I W N L H A D A H D F D S Q T - S D L E D I S R K I F S A H F G H L A V V F V W L S G M Y F H G A K F S N Y E A W L N 

M A T A D A A F N P - - - - - - - - - - - R A Q V F E W F K - D K V P A T R G A V L K A H I N H L G M V A G F V S F V L V H - - - - - - - - - - - - 

M A T A E M A F N P - - - - - - - - - - - R A Q V F E Y F K - D K V P A T R G A V L K A H I N H L G N I S A M V S F I L V H - - - - - - - - - - - - 

M A T A E M A F N P - - - - - - - - - - - R A Q V F E Y F K - D K V P A T R G A V L K A H I N H L G N V A A M V S F I L V H - - - - - - - - - - - - 

M A S F S S - - - - - - - - - - Y A N G V K R W Y Q K L E L P M P P E R I F G A H M M L I G G L A C L I G T Y F F A S M T M W N - D G Y V N 

M A D Q A N P T G V M P K G K V P P P K G K V P P P K G N - G S P G S A S V V Q E K D A A K M K R F L F Q R T E T R S T K W Y Q I F D T D K L 

M A E Q V N P A G V K P K G T V P P P K G S A P S P K A S - G A S G G P S V I K E Q D A A K M R R F L F Q R T E T R S T K W Y Q V F D T D K I 

M A E Q V K P A G V K P K G T V P P P K G N A P A P K A N - G A P G G A S V I K E Q D A A K M R R F L F Q R T E T R S T K W Y Q I F D T E K L 

M A E Q V N P A G V K P K G S V P P P K G N A P S P K G N - G A S G G P S V I K E Q D A A K M R R F L F Q R T E T R S T K W Y Q I F D T E K L 

M A D Q A N P T G V K P K G Q V P P P K G K V P P P K G N G A P A G G A S I I K E K D A A K M R R F L F Q R T E T R S T K W Y Q V F D T D K V 

M A E Q V N P A G V K P K G S V P P P K G N A P S P K G N - G A P G G P S V I K E Q D A A K M R R F L F Q R T E T R T T K W Y Q V F D T D K L 

M A E Q V N P A G V K P K G T V P P P K G N A P S P K G N - G A A G A P S V I K E Q D A A K M R R F L F Q R T E T R S T K W Y Q I F D T D R V 

M A E Q V N P A G A V P K A K V P P P K G N A P S P K G D - G T S G A P S V I K E Q D A A K M R R F L F Q R T E T R T T K W Y Q I F D T D K I 

M A D Q A N P T G V K P K G Q V P P P K G K V P P P K G N G A P A G G A S I I K E K D A A K M R R F L F Q R T E T R S T K W Y Q V F D T D K V 

M A E Q V K P A G V K P K G T V P P P K G N A P A P K A N - G A P G G A S V I K E Q D A A K M R R F L F Q R T E T R S T K W Y Q I F D T E K I 

M P S F L P C L R E R R D S M T I S P P E R E P K V V D D P V P T S F E K F M A . Q . N P A G V K P K G V P P P K G . P P K G . G S V . K E . D A A K M . R F L F Q R T E T R . T K W Y Q . F D T . K . 



















130 140 150 160 170 180 190 200 210 220 230 240 

D P V N T R P I A H A I W D P Q F G K A A V D A F T Q A G A S N P V D I - - - - - A Y S G V Y H W W Y T I G M R T N G D L Y Q G A I F L L I L A S L A L F A G W L H L Q P K F R P S L S W F K N A E S R L N H H L A G - - L F G V S S L A W A G 

D P L N V R P I A H A I W D P H F G Q G A I D A F T Q A G A S N P V N I - - - - - A Y S G V Y H W F Y T I G M T T N A D L Y Q G A V F L L I L S S L F L F A G W L H L Q P K F R P S L A W F K D A E S R L N H H L A G - - L F G V S S L A W T G 

D P T G I K P S A Q V V W P I V G - - - Q G I L N G D V G G G F H G I Q - - - - - I T S G L F Q L W R A S G I T N E F Q L Y C T A I G G L V M A G L M L F A G W F H Y H - K R A P K L E W F Q N V E S M L N H H L A G - - L L G L G S L A W A G 

N P T V I K P S A Q V V W P V V G - - - Q G I L N G D V G G G F S G I Q - - - - - I T S G L F Y L W R A A G F T N S Y Q L Y V T A I G G L V M A A L M V F A G W F H Y H - K A A P K L E W F Q N A E A M M N H H L S V - - L L G C G S L G W T G 

- - - - - - - - - H L S W L S - - - - - - D Q V L F A P T P - - - - - - - - - - - - - - I F Y A R L Y Q L G L D A S - A R S A D A L M V A R L H L L A A I I F W I I G H - I K T P R E D E F L K N V S F G K T L V A Q F H F L A L V A T L W G M 

- - - - - - - - - H L S W D P A T - - - - Q G V L W A P A T - - - - - - - - - - - - - - M F Y A R L Y Q L G M D A T - A L S P D A L F V A R M H L L A A I I L W G V G H - I K S P A E E K F L E K V T M G K S L V A Q F H F F A L I A T L W G L 

- - - - - - - - - H L S W D P A T - - - - Q G V L W A P A T - - - - - - - - - - - - - - M F Y A R L Y Q L G L D A T - A L S P D A L F V A R M H L L A A I I L W G F G H - V K S P A E E K F L E K V T M G K A L V A Q F H F F A L I A T L W G L 

L T L R P R L I A L G I Y D P Y D T E Q I Q R V W L P L I G E F S T S - - - - - - - K L P F F G Q Y P L T M T D F R L F G W G C F H I G L G L W L V Y A G A A H Y Y G A R G G A T I G E I F W L L P Y V P G L K G L C Q I K W F T P D G P W Y K 

D D E Q V V G - A H L A L L G V L G F V M G I Y Y L S G M Q V M P W G - - - A P G F H D N W F Y L T I K P R M V S L G I D T Y S P K T E D L M A A S T K L M G W A I L H - F V S G S I L L F G - G W R H W T H N L T N P F T G R A G N F R D F R 

D D E Q V V G - G H L A L L G V L G F L M A I Y Y F S G I Q V F P A W L G G M P G F H D N W F Y L T I K P R M V S L G I D T Y S T Q T A D L Q Q A A T N L I G W A A F H - F L S G S I L L F G - G W R H W T H N L T N P F T G R A G N F R D F R 

D D E Q V V G - G H L A L L G V L G F I M G I Y Y I S G I Q V F P W G A P G - - - F H D N W F Y L T I K P R M V S L G I D T Y S T K T A D L E A A G A R L L G W A A F H - F L V G S V L I F G - G W R H W T H N L T N P F T G R C G N F R D F R 

D D E Q V V G - G H L A L L G V M G F L M A I Y Y I S G I Q V F P W G A P G - - - F H D N W F Y L T I K P R M V S M G I D T Y S T R S A D L E Q A A T N L L G W A L L H - V I S G S I L L F G - G W R H W T H N L T N P F T G R A G N F R D F R 

D D E Q V V G - G H L A L L G V L G F I M G I Y Y L S G M Q V L P W N G - - A P A F H D N W F Y L T I K P R M V S L G I D T Y S P R T E D L I A A S W K L M G W A I L H - F V S G S I L L F G - G W R H W T H N L T N P F T G R A G N F R E F R 

D D E Q V V G - G H L A L L G V M G F L M A I Y Y I S G I Q V F P G G Y A G - - - F H D N W F Y L T I K P R M V S L G I D T Y S T K T A D L E Q A A I N L L G W A L L H - V I S G S I L L F G - G W R H W T H N L T N P F T G R A G N F R D F R 

D D E Q V V G - A H L A L L G F L G Y L M A I Y Y I S G V Q V F P W G A P G - - - F Q D N W F Y L T I K P R M V S L G I D T Y S T K T E D L Q W A A S N L L G W A L F H - I I S G S I L I F G - G W R H W T H N L T N P F T G R A G N F R D F R 

D D E Q V V G - G H L A L L G V L G F L M A I Y Y I S G I Q V F P A F L G G G P G F H D N W F Y L T I K P R M V S L G I D T Y S T N T A D L Q Q A A T N L L G W A A F H - F L S G S I L L F G - G W R H W T H N L T N P F T G R A G N F R D F R 

D D E Q V V G - G H L A L L G V L G F I M G I Y Y L S G M Q V L P W N G - - A P A F H D N W F Y L T I K P R M V S L G I D T Y S P R T E D L I A A S W K L M G W A I L H - F V S G S I L L F G - G W R H W T H N L T N P F T G R A G N F R E F R 

D D E Q V V G - G H L A L L G V L G F I M A I Y Y I S G I Q V F P W G A P G - - - F H D N W F Y L T I K P R M V S L G I D T Y S T K T A D L E W A G T K L L L W A G Y H - F I A G S V L I F G - G W R H W T H N L T N P F T G R C G N F R D F R 

D D E Q V V G I G H L A L L G V . G F . M . I Y Y . S G . Q V F P . P G A P G F H D N W F Y L T I K P R M V S G I D T Y S . . D L A . L . G W A . . H P . S G S . L . F G G W R H W T H N L T N P F T G R . G N F R . F R 



















250 260 270 280 290 300 310 320 330 340 350 360 

H L I H V A I P E S R G - Q H V G W D N F L S T M P H P A G L A P F F T G N W G V Y A Q - - N P D T A S H V F G T A Q G A G T A I L T F L G G F H P Q T E S L W L T - D M A H H H L A I A V L F I V A G H M Y R T Q F G I G H S I K E M M D A K 

H L V H V A I P E S R G - V H V G W D N F L S V K P H P A G L M P F F T G N W G V Y A Q - - N P D T A G H V F G T S Q G A G T A I L T F L G G F H P Q T E S L W L T - D I A H H H L A I A V L F I I A G H M Y R T N V R I G H S I K E I L N A H 

H Q I H V S L P I N K L - L D A G V A A K D I P L P H E F I L N P S L M A E L Y P K V D W G F F S G V I P F F T F N W A A Y S D F L T F N G G L N P V T G G L W L S - D T A H H H L A I A V L F I I A G H M Y R T N W G I G H S L K E I L E A H 

H L I H V S L P V N K L - L D S G V A A K D I P L P H E F F D - A S V M A E L Y P - - - - S F A Q G L K P F F T L D W A A Y S D F L T F K G G L N P T T G S L W L S - D V A H H H L A I A V L F I I A G H M Y R T G F G I G H S M K E I L E A H 

H M A Y I G V R G A N G G I V P T G L S F D M F G P I T G A T L A G N H V A F G A L L - - - F L G G V F H H F A - - - G F N T K R F A F F E K D W E A V L S V S A Q - V L A F H F A T V V F A M I I W N R P D Q P I L S F Y F M Q D Y A L S N Y 

H M A F Y G I L G P E G K L A P T G L S F D M F G P I T P A S M A G N H V A F G A V F - - - F L G G I F H Y F A - - - G F N T K K F A F F E K D W E A V L S V S C Q - V L A F H F A T V V F A M I I W Q H P - - - Q L G F G F M R E Y A V S Q Y 

H M A F Y G I L G P S G K L E P T G L S F D M F G P I T P A T M A G N H V A F G A V F - - - F L G G I F H Y F A - - - G F N T K R F A F F E K D W E A V L S V S C Q - I L A F H F A T V V F A M I I W Q H P - - - Q L G F G F M R E Y A V S Q Y 

V G L P W G S F A N T P W R I L R R T Y A D A L G A H T I Y I G L L F - F I W G F V L W F V L D K P P V P L Q P - - - A Q V M T P N G P M P I E Q A P F P Y G W F D P Y L N E V M H P M N T I N G E T T M S F V W G V L F V V L G A Y W W Y R P 

F L G K F G D A V F Q G - - T S A K T Y K D A L G P H T V Y M A L L F - L G W G F M L W G V L G I A P I P D F Q T S N S E T F M S F V W F V V F F A L G L Y W W K N P P N A A T H L N D D M K A A F S V H L T A I G Y V N I A L G I I A F V A F 

F L G K F G D M V F N G - - T S A K T Y K D A L G P H A V Y M S L L F - L G W G L L M W F V L G F A P V P D F Q T I N S E T F M S F V W F V A F F A L G I Y W W N N P P N A A I H L N D D M K A A F S V H L T A I G Y I N I A L G V I A F V A F 

F L G K F G D V V F N G - - T S A K S Y K E A L G P H A V Y M S L L F - L G W G I V M W A I L G F A P I P D F Q T I N S E T F M S F V F A V I F F A L G I Y W W N N P P N A A I H L N D D M K A A F S V H L T A I G Y I N I A L G C I A F V A F 

F L G K F G D V V F S G - - T S A K S Y K D A L G P H A V Y M S L L F - L G W G L I M W L A L G W A P V P D F Q T I N S E T F M S F V W F V V F F A L G I Y W W N N P P N A A T H L N D D M K A A F S V H L T A I G Y I N I A L G V I A F V A F 

F L G K F G D A V F Q G - - T S A K T Y K D A L G P H A V Y M S L L F - L G W G L M L W I V L G F A P I P D F Q T I N S E V F M S F V W F L V F F A L G L Y W W K N P P N A A I H L N D D M K A A F S V H L T A I G Y V N I A L G I I A F V A F 

F L G K F G D M A F N G - - T S A K T Y K D A L G P H T V Y M S L L F - L G W G L V M W F A L G I P P V P D F Q T I N S E P F M S F V F A V L F F A L G I Y W W N N P P N A A I H L N D D M K A A F S V H L T A I G Y I N I A L G V I A F V A F 

F L G K F G D V V F S G - - T S A K S Y K D A L G P H T V Y M S L L F - L G W G F V M W F V L G F A P I P D F Q T I N S E T F M S F I F A V I F F A A G I Y W W N N P P N A A T H L N D D M K A A F S V H L T A I G Y I N I A L G C I A F V A F 

F L G K F G D A V F N G - - T S A K T Y K D A L G P H A V Y M S L L F - L G W G L L M W L V L G Y A P V P D F Q T I N S E T F M S F V W F A V F F A L G L Y W W N N P P N A A I H L N D D M K A A F S V H L T A I G Y I N I A I G V I A F V A F 

F L G K F G D A V F Q G - - T S A K T Y K D A L G P H A V Y M S L L F - L G W G L M L W I V L G F A P I P D F Q T I N S E V F M S F V W F L V F F A L G L Y W W K N P P N A A I H L N D D M K A A F S V H L T A I G Y V N I A L G I I A F V A F 

F L G K F G D M V F S G - - T S A K S Y K E A L G P H A V Y M S L L F - L G W G L V M W L I L G F A P I P D F Q T I N S E T F M S F V F A V I F F A L G I Y W W N N P P N A A I H L N D D M K A A F S V H L T H R - L H Q H R A W V I A F V A F 

F L G K F G D V F G T S A K . Y K D A L G P H V Y M S L L F L G W G . . W V L G . P . P D F Q T I N S E F M S F . F . . F F A . G . Y W W N P P N A A H L N D D M K A A F S V H L T A I G . . . . . . G I A F V A F 



















370 380 390 400 410 420 430 440 450 460 470 480 

D F F G T K V E G P F N M P H Q G I Y E T Y N N S L H F Q L G W H L A C L G V I T - - - S L V A Q H M Y S - L P P - - Y A F I A Q D H T T M A A L Y T H H Q Y I A G F L M V G A F A H G A I F L V R D Y D P A Q N K G N V L D R V L Q H K E A I 

Q P P - - - - S G K L G A G H K G L Y D T V N N S L H F Q L G L A L A S L G V I T - - - S L V A Q H M Y S - L P S - - Y A F I A K D Y T T Q A A L Y T H H Q Y I A G F L M V G A F A H G A I F F V R D Y D P E A N K D N V L Y R M L E H K E A L 

K G P - - - - - - F T G A G H K G L Y E V L T T S W H A Q L A I N L A M M G S L S - - - I I V A Q H M Y A - M P P - - Y P Y L A T D Y P T Q L S L F T H H M W I G G F L V V G G A A H G A I F M V R D Y D P A M N Q N N V L D R V L R H R D A I 

K G P - - - - - - F T G E G H K G L Y E I L T T S W H A Q L A V N L A L L G S L T - - - I V I A H H M Y S - M P P - - Y P Y M A T D Y G T Q L S L F T H H T W I G G F L I V G A G A H G A I F M V R D Y D P A K N V N N L L D R V I R H R D A I 

A A P - - - E I R E I A S Q N P G F L I K Q V I L G H L V F G V M F W I G G V F H G - - A H F M L R A T N - D P K - - L A E A L K D F K M L K R C Y D H D F Q K K F L A L I M F G A F L P I F V S Y G - - - I A T H N T I A D L H H L A K A G M 

A G P - - - E L K M I A Q G N P G L L V K Q A I L G H L V M G I M F W I G G V F H G - - A H F M L R V L N - D P K - - M A E E M K D F K F I K R C Y D H E F Q K K F L A L I M F G A F L P I F V A Y G - - - I A T H N T I A D I H A A A K T G I 

A G P - - - E L K M I A Q S N P G L L V K Q A I L G H L V M G I M F W I G G V F H G - - A H F M L R V L N - D P K - - L A E E M K D F K F I K R C Y D H E F Q K K F L A L I M F G A F L P I F V S Y G - - - I A T H N T I A D I H A A S K T G L 

P R S - - I N I T H L E D T K A V F H V H L T S I G Y V S F A L A I V G F L A L R N H P S Y L I L N D M N V I I Y G K K I V N P G R M I H N M I T F N H V Q V G L L Y V A A G V F H G G Q Y L H G L N I S G A Y K Q A R S K F I T W F Q N P D L 

Q Q D - S F A P Y Y A E L D K L V F Y I Y G E P F N R V S F D F V Q E G G K V I A G S K E F E E F A A Y A I L P K N G E S F G M A R V V I N L I T F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q I N G L Y S Q I K S I W V A K G R D Q D L 

Q Q P - S F A P Y Y K E L D K L V F Y L Y G E P F N R V S Y D F V Q E G G R I V S G S K E F S E F A P F A I L P K N G A S F G M S R V V I N L I T F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q L N G L Y N Q I K S I W I T K G R N Q E V 

Q Q P - S F A P Y Y K E L D K L V F Y L Y G E P F N R V S F N F V E Q G G K V I S G A K E F A D F P A Y A I L P K S G E A F G M A R V V T N L I V F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q L N G M Y N Q I K S I W I T K G R D Q E V 

Q Q P - S F A P Y Y K E L D N L V F Y L Y G E P F N R V S Y D F V Q E G G Q I V S G S K E F T E F A P Y A I L P K N G E A F G M S R V V I N L I T F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q L N G L Y N Q I K S I W I T K G R D Q E V 

Q Q D - S F A P Y Y A E L D K L V F Y I Y G E P F N R V S F D F V E Q G G K V L S G S K E F A E F P A F A I L P K N G E S F G M A R V V I N L I V F N H I I C G V L Y V F A G V Y H G G Q Y L L K V Q I N G L Y S Q I K S I W V A K G R D Q E L 

Q Q P - S F H A Y Y K E L D N L V F Y L Y G E P F N R V S Y D F V Q E G G R I I S G K K E F V E F A P Y A I L P K N G Q A F G M S R V V I N L I T F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q L N G L Y N Q I K S I W I T K G R D Q E V 

Q Q P - S F A D Y Y K A L D S L V F Y I Y G E P F N R V S Y D Y V E A G G R I V S G S K E F A D F P A Y A I L P K D G A A F G M S R T V I N L I V F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q L N G L Y S Q I K S V F I T K G R D Q E V 

Q Q P - S F A P Y Y Q E L D N L V F Y L Y G E P F N R V S Y D F V Q E G G R I V S G S K E F A E F A P F A I L P K N G Q S F G M S R V V I N L I S F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q L N G L Y N Q I K S I W I T K G R D Q E V 

Q Q D - S F A P Y Y A E L D K L V F Y I Y G E P F N R V S F D F V E Q G G K V L S G S K E F A E F P A F A I L P K N G E S F G M A R V V I N L I V F N H I I C G V L Y V F A G V Y H G G Q Y L L K V Q I N G L Y S Q I K S I W V A K G R D Q E L 

Q Q P - S F A P Y Y K E L D K L V F Y I Y G E P F N R V S F N F V E Q G G K V I S G A K E F A E F P A Y A I L P R N G E S F G M A R V V T N L I T F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q L N G I Y N Q I K S I W I T K G R D Q E V 

Q Q P G S F A Y Y E L D . L V F Y . Y G E P F N R V S . . V G G V . S G S K E F . . F Y A I L P K N G . F G M R V N L I F N H I I C G V L Y V F A G V Y H G G Q Y L L K . Q . N G . Y N Q I K S . . I K G R Q . . 



















490 500 510 520 530 540 550 560 570 580 590 600 

I S H L S W V S L F L G F H T L G L Y V H N D V V V A F G T P E K Q I L I E P V F A Q F I Q A A H G K L L Y G F D T L L S N P D S I A S T A W P N Y G N V W L P G W L D A I N S G T N S L F L T 

I S H L S W V S L F L G F H T L G L Y V H N D V V V A F G T P E K Q I L I E P V F A Q F V Q A A S G K A L Y G F D V L L S N A D S I A S N A S A A Y - - - - L P G W L D A I N S G S N S L F L N 

I S H L N W V C I F L G F H S F G L Y V H N D T M R A F G R P Q D M F S D T G I Q L Q P V F A Q W V Q N L H T L A P G G T A P N A A A T A S V A F G G D V V A V G G K V A M M P I V 

I S H L N W V C I F L G F H S F G L Y I H N D T M R A L G R P Q D M F S D S A I Q L Q P I F A Q W I Q N I H T L A P G G T A P N A I A S A S Q V F G G D V V A I G N K V A L M P I T 

F A N M T Y I N I G T P L H D A I F G S H G 

F A H M T Y I N I G T P L H D A V F G S K G 

F A H M T Y I N I G T P L H D A I F G S K G 

Q T K I V G T T M F V S F V T V V F - G Y G M I C W N T G A E L D L N F G I Y Q F R S F R A I Q M D G E A G N I G Y R V F R P N N P W D P T A G G D W V K N P D G T A K L V K A R N L Q V G D R I L N E E L G I G S S P T Y S F T T 

Q V K V L G T I M A L C F A T M L S - V Y A V I V W N T I C E L N I F G T N - - - - I M M S F Y W L K P L P F L H W M F E D P 

Q V K I L G T V M A L C F A T M L S - V Y A V I V W N T I C E L N I F G T N - - - - I M M S F Y W L K P L P M F Q W M F A D P 

Q V K I L G T V M A L C F A T M L S - V Y A V I V W N T I C E L N I F G T N - - - - I T M S F Y W L K P L P I F Q W M F A D P 


Q V K I L G T I M A L C F A T M L S - V Y A V I V W N T I C E L N F F G T N - - - - I M M S F Y W L K P L P F L H W M F E D P 

Q V K I L G T V M A L C F A T M L S - V Y A V I V W N T I C E L N I F G T N - - - - I M M S F Y W L K P L P I F Q W M F T D P 

Q V K I L G T V M A L C F A T M L S - V Y A V I V W N T I C E L N L F G T N - - - - I M M S F Y W L K P L P M F Q W M F N D P 


Q V K I L G T I M A L C F A T M L S - V Y A V I V W N T I C E L N F F G T N - - - - I M M S F Y W L K P L P F L H W M F E D P 

Q V K I L G T V M A L C F S T M L S - V Y A V I V W N T I C E L N L F G T N - - - - I T M S F Y W L K P L P I F Q W M F A D P 

Q V K I L G T . M A L C F . T M L S Y V Y A V I V W N T I C E L N F G T N F I M M S F Y W L K P L P F W M F D P N I A S G V G A N S L F L I L N E E L G I G S S P T Y S F T T 

I 

II 

III IV 

V VI 

VII 

Figure 2S

Figure 3S 

PscA/PshA/PsaA/PsaB C-terminal Alignments 













Pld. phaeo. PscA 




Chl. phaeobacteroides BS1 PscA 


10 20 30 40 50 60 70 80 90 100 

I G P G D F L V H H A I A L G L H T T T L I L V K G A L D A R G S K L M P D - - - K K D F G Y A F P C D G P G R G G T C D I S A W D A F Y L A M F W M L N T I G W V T F Y W H W K H L G V W E G N V A Q 

I G P G D F L V H H A I A L G L H T T T L I L V K G A L D A R G S K L M P D - - - K K D F G Y S F P C D G P G R G G T C D I S A W D A F Y L A M F W M L N T L G W L T F Y W H W K H L G I W Q G N V A Q 

L G T A D F M V H H I H A F T I H V T V L I L L K G V L F A R S S R L I P D - - - K A N L G F R F P C D G P G R G G T C Q V S G W D H V F L G L F W M Y N C I S V V I F H F S W K M Q S D V W G T V A P 

L G T A D F M V H H I H A F T I H V T V L I L L K G L L Y S R S S R L V P D - - - K G Q L G F R F P C D G P G R G G T C Q V S G W D H V F L G L F W M Y N S L S I V I F H F S W K M Q S D V W G T I L P 

T V S D F V A A H A I A G G L H F T M V P L W R M V F F S K V S P W T T K V G M K A K R D G E F P C L G P A Y G G T C S I S L V D Q F Y L A I F F S L Q V I A P A W F Y L D G C W M G S F V A T S S E 

S I S E F V A A H A I A G G L H F T M V P M W R M V F F S K V S P W T T K V G M K A K R D G E F P C L G P A Y G G T C S I S L V D Q F Y L A I F F S L Q V I A P A W F Y I D G C W M G S F V A V A Q P 

S I S E F V A A H A I A G G L H F T M V P M W R M V F F S K V S P W T T K V G M K A K R D G E F P C L G P A Y G G T C S I S L V D Q F Y L A I F F S L Q V I A P A W F Y I D G C W M G S F V A V A A P 

E I N D A I F F W L M I G G W L F G F I P L L R I A F H N Y Q S P W Y R D F E W R K Q S P - D F P C I G P V K G G T C G V S I Q D Q M W F C I L F S I K P L S A I A W Y L D G G W I A T M M A R G - - 

S I N D W V M A H V I V A G S L F S L I A L A R I A F F S H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G V K G L S A V C W Y I D G A W I A S M M Y G V P - 

S I N D W V M V H V I V A G S L F S L I A L V R I A F F A H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G V K G L S A V C W Y I D G A W I A S M M Y G V P - 

S I N D W V M A H V I T A G S L F S L I A L V R I A F F A H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G I K G L S A V C W Y I D G A W I A S M M Y G V P - 

S I N D W V M A H V I T A G S L F S L I A L V R I A F F A H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G V K G L S A V C W Y I D G A W I A S M M Y G V P - 

S I N D W V M V H V I V A G S L F S L I A L V R I A F F A H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G I K G L S A V C W Y I D G A W I A S M M Y G V P - 

S I N D W V M A H V I T A G S L F S L I A L V R I A F F S H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G V K G L S A V C W Y I D G A W I A S M M Y G V P - 

S I N D W V M V H V I T A G S L F S L I A L V R I A F F S H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G I K G L S A V C W Y I D G A W I A S M M Y G V P - 

S I N D W V M V H V I V A G S L F S L I A L V R I A F F A H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G V K G L S A V C W Y I D G A W I A S M M Y G V P - 

S I N D W V M A H V I V A G S L F S L I A L A R I A F F S H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G V K G L S A V C W Y I D G A W I A S M M Y G V P - 

S I N D W V M A H V I T A G S L F S L I A L V R I A F F A H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G I K G L S A V C W Y I D G A W I A S M M Y G V P - 

S I N D W V M . H V I . A G S L F S L I A L V R I A F F H T S P L W D D L G L K K N S Y S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G . K G L S A V C W Y I D G A W I A S M M Y G V P P 



















110 120 130 140 150 160 170 180 190 200 

F N - - - - - - - - - - - - - - E S S T Y L M G W L R D Y L W L N S S Q L I N G Y N P F G T N N L S V W A W M F L F G H L V W A T G F M F L I S W R G Y W Q E L I E T - - - - L V W A H E R T P L A N L 

F N - - - - - - - - - - - - - - E K S T Y L M G W F R D Y L W A N S A Q L I N G Y N P Y G V N N L S V W A W M F L F G H L V W A T G F M F L I S W R G Y W Q E L I E T - - - - I V W A H E R T P L A N I 

D G T V - - - S H I T G G N F A Q S A I T I N G W L R D F L W A Q A S Q V I G S Y G - - - - S A L S A Y G L L F L G A H F I W A F S L M F L F S G R G Y W Q E L I E S - - - - I V W A H N K L K V A P A 

D G S V - - - S H V T G G N F A T S A I T I N G W L R D F L W A Q S S Q V I N S Y G - - - - S A L S A Y G I M F L A G H F V F A F S L M F L F S G R G Y W Q E L I E S - - - - I V W A H N K L K L A P A 

V Y K Q A A E L F K A N P T W F S L H A V S N F T S E G Y F S Y I I S Q T T R M F K Y Y - - - - D G H L V Q A L L G A H F I W A F T F S M L F Q Y R G S R D E G A - - - - M V L K W A H E Q V G L G F A 

N N E I Y Q A A L A A Y N A H N P L H Q L S P L T N M G Y F S Y I I Q Q T T A M F S R Y - - - - D G H M I Q A L L G A H F I W A F T F S M L F Q Y R G S R D E G S - - - - M V L K W A H E Q V G V G F A 

Y N D I Y Q A A L A T F N S H N P L H Q L S P L T N M G Y F S Y I I Q Q T T A M F S R Y - - - - D G H M I Q A L L G A H F I W A F T F S M L F Q Y R G S R D E G A - - - - M V L K W A H E Q V G V G F A 

- - - - - - - - - - - - - N E A Y Y L T H N I S H T G G V F L Y M W N E T T W I W T - - - - - - D N H L T A M L L L G H L I W F V S F A L W F K D R G S R A E G G D I Q S R W V R L M G K R L G I K T L 

- - - - - - - - A A D A K S W D A V A G L S H H Y S A G I F Y Y F W T E T V T I F S - - - - - - S S H L S V I L M I G H L I W F I S F A V W F E D R G S R L E G A D I Q T R T V R W L G K K F L N R D V 

- - - - - - - - A A D A K A W D S V A G L A H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S S H L S T I L M I G H L V W F I S F A V W F E D R G S R L E G A D I Q T R T I R W L G K K F L G R D V 

- - - - - - - - A A D A K A W D S I A H L H H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S S H L S T I L M I G H L V W F I S F A V W F E D R G S R L E G A D I Q T R T I R W L G K K F L N R D V 

- - - - - - - - V A D A K A W D S V A G L Q H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S S H L S T I L M I G H L I W F I S F A V W F E D R G S R L E G A D I Q T R T V R W L G K K F L N R D V 

- - - - - - - - A A D A K A W D S V A G L Q H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S S H L S T I L M I G H L V W F I S F A V W F E D R G S R L E G A D I Q T R T I R W L G K K F L N R D V 

- - - - - - - - V A D A K A W D S V A G L A H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S S H L S V I L M I G H L I W F I S F A V W F E D R G S R L E G A D I Q T R T I R W F G K K F L N R D V 

- - - - - - - - A A D A K A W D S V A G L Q H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S P H L S T I L M I G H L V W F I S F A V W F E D R G S R L E G A D I Q T R T I R W L G K K F L N R D V 

- - - - - - - - A A D A K A W D S V A G L A H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S S H L S T I L M I G H L V W F I S F A V W F E D R G S R L E G A D I Q T R T I R W L G K K F L G R D V 

- - - - - - - - A A D A K A W D A V A G L S H H Y S A G I F Y Y F W T E T V T I F S - - - - - - S S H L S V I L M I G H L V W F I S F A V W F E D R G S R L E G A D V Q T R T I R W L G K K F L N R D V 

- - - - - - - - A A D A K A W D S I A H L Q H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S S H L S T I L M I G H L V W F I S F A V W F E D R G S R L E G A D I Q T R T I R W L G K K F L N R D V 

N Y Q A A A D A K A W D . . A . L H H Y . G I F Y Y F W T E T V T I F S Y G S S H L S I L M I G H L V W F I S F A V W F E D R G S R L E G A D I Q T R T I R W . G K K F L . R D V 



















210 220 230 240 250 260 270 280 290 300 

V R W K D K P V A L S I V Q A R L V G L A H F S V G Y I L T Y A A F L I A S T A A K F G 

V R W K D K P V A L S I V Q A R L V G L A H F T V G Y V L T Y A A F L I A S T S G K F G 

I Q P R - - - - A L S I I Q G R A V G V A H Y L L G G I A T T W A F F L A R I I S V G 

I Q P R - - - - A L S I V Q G R A V G V A H Y L L G G I V T T W A F F L A R S L S I G 

G K V Y - - N R A L S L K E G K A I G T F L F F K M T V L C M W C L A M V 

G K V Y - - N R A L S L K E G K A I G T F L F F K M T I V C M W C L A M V 

G K M Y - - N R A L S L K E G K A I G C F L F F K M T I V C M W A L A M V 

Q E V R - - F P V S N L A T A K L W G T V F F Y T G T F V L V F L Y F A D G F F Q N R 

T F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F I A N G F Y Q T N T P A L P P V G D A A I S G Q Q L L T Q V V D F I V K L I A 

N F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F M A N G F Y Q T D S P L P P T V S D A A V S G Q V L L T Q V V D Y L M K L I A 

N F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F L A N G F Y Q T N S P L P P P V S H A A V S G Q Q M L A Q L V D T L M K M I A 

T F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F I A N G F Y Q T N S P L P P T V G D A S L S G Q Q M L T Q V V D Y L M K L I A 

T F R - - - F P V L T I S D S K M A G T F L Y F S G V F M L V F L F I G N G F Y Q T D S P L P P Q V G D A S V S G Q V M L T Q V V D Y L M K L I A 

T F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F I G N G F Y Q T D S P L P P P V A E A A V S G Q Q M L T Q V V D F L I K L V A 

T F R - - - F P V L T I S D S K M A G T F L Y F G G V F M L V F L F I G N G F Y Q T D S P L P P Q V G D A S V S G Q M M L T Q V V D Y L L K L I A 

Q F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F L A N G F Y Q T D S P L P P T V S D A A V S G Q A L L T Q V V D Y L M K L I A 

Q F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F I A N G F Y Q T N T P A L P P V G D A A V S G Q Q L L T Q V V D F I M K L I A 

N F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F I A N G F Y Q T N S P L P P P V S H A A V S G Q Q M L A Q L V D T L M K M I A 

F R D K F P V L T I S D S K L A G T F L Y F . G T F M L V F L F . . N G F Y Q T S P L P P V D A A V S G Q Q M L T Q V V D L M K L I A 

VIII 

IX 

X 

XI 

F X 

P840 

P780 

P700

Figure 4S 

Cab. thermophilum PscB 

Chl. tepidum PscB 

Chl. limicola 245 PscB 

Chl. phaeobacteroides PscB 

Ptc. aestuarii PscB 





















PscB Alignments 

10 20 30 40 50 60 

M A A E E K A E M A K D A S A E A P A E K A A T K A A A K T A A K S - - - - - - - - - - 

M A E P V E N K N Q A P A P G A K V P P K G A P A A P K A G A P A A P K G P V A P K A G A P A A K T G A S A A K Q A G K 

M A E P V K Q P D I S P K P A S K A A P A A P K A S V A P K A A T P A A K Q D Q A K P K A S S P K P - - - - - - S S G K 

M A E P V K Q P D V P P A P V S K A A P A A P K A A P K A A S P A A K Q G E - V K P K I S A A P K Q - - - - - - T S G K 

M A D P V K K Q E - - - - - T A S G P A K A K P A A P K A K A D A P K G K P A P K A G G A S S L V K G - - - - - - - - - 

M A E P V K . . P A P . K A A P A A A P K A A A P K . K . . A . A . K G A S A A K S G K 

70 80 90 100 110 120 

- - - - - - - - - - - - - - - - - A A A K A P A K A A A K A A P P P I P G - - - - - - - - - - - - P P S R - - - - - - - 

P R L A S L G V T L G R S G V R Q E S A L P Y V K P K A V P P P K P A A P - A A K G A P A P K G A P A A P - - - - - - - 

L R L E A L N V N L G R T G I R Q E S A L P V K K A A P K A A P K P A P G - A K P A A P A A K A A P A V G - - - - - - - 

I R L E A L N V N L G R T G I R Q E S A L P V K K A P P K A A P K P A P G - G A K P A P G A K P A P K T A G A P A G T P 

- R L D S L D V N L G R T G R R M E S A L P V V R P K P K A A P K L A P K G A P K G A P A G K A V P K G A P A A K G - - 

R L . L V N L G R T G . R Q E S A L P V . K A P K A A P K P A P G G A K . A P A . K . A P . . A G T P 

130 140 150 160 170 180 

- - - - - L S R P K T A A P K K K R E R Q I Y T I I E E L C I G C G F C T D E C P P K V N A I L P R D V E A V L D G G E 

- - A A K A A P G A P V A K A A P K A K K H Y F I I E N L C V G C G L C L D K C P P K V N A I G Y K F Y G D V Q E G G F 

- - A A P A P K G A P V A K A A P R A K K H Y F I I E N L C V G C G L C L D K C P P K V N A I G Y K F Y G D V Q E G G F 

V A K S A P A A G T P L A K A A P R A K S H Y F I I E N L C V G C G L C L D K C P P K V N A I G Y K F Y G D V Q E G G F 

- A P A A K P A A K P I A K K A P R P K K H Y F I L E N L C V G C G L C L D T C P P K V N A I G Y K F Y G D V Q E G G F 

V A A . G P . A K A A P R A K K H Y F I I E N L C V G C G L C L D K C P P K V N A I G Y K F Y G D V Q E G G F 

190 200 210 220 230 240 

T Y W I D Q T R C I S C S L C F V A G T C P T D A V V F T E G G V S R T Q Y M E D Y M H I E L V D E P Y W R T R D E L S 

R C Y I D Q A A C I S C S A C F S G D E C P S G A L I E V L P D G E V L D F S Y T P P E R L D F D L R F L H R F H R E A 

R C Y V D Q D A C I S C S A C F S G D E C P S G A L I E V L P D G Q I L D F S Y T P P E R L D F D L R F L H R F H R E A 

R C Y I D Q D A C I S C S A C F S T D E C P S G A L I E V L P D G Q V L D F S F T P P E R L D F D L R F L H R F H R E V 

R C Y I D Q D A C I S C A A C F S S D E C P S G A L I E I L P T G E V L D F T Y T P P E R L D F D L R F L H R F H K E S 

R C Y I D Q D A C I S C S A C F S . D E C P S G A L I E V L P D G V L D F S Y T P P E R L D F D L R F L H R F H R E . 

R L G S I L * 

R 

R 

R 

R 

R L G S I L 

250 260 270 280 290 300

Figure 5S 

FmoA Alignments 

10 20 30 40 50 60 

Cab. thermophilum FmoA 

M G S K L T T A Y Y D L D L D A T G - - - - W G T L R A E A R I S N V P S A S P L L P I D G E L K L E A K - - K 

Chl. tepidum FmoA M A L F G S N D V T T A H S D Y E I V L E G G S S S W G K V K A R A K V N - A P P A S P L L P A D C D V K L N V K P L D 

Chl. limicola FmoA 

M A L F G T K D T T T A H S D Y E I I L E G G A S S W G K V K G R A K V N - V P P A S P L L P A D C N V K I N V K P L D 

Chl. phaeobacteroides FmoA M A L F G T K D T T T A H S D Y E I I L E G G S S S W G K V K G R A K V N - V P P A S P L L P A D C N V K I N V K P L D 

Ptc. aestuarii FmoA M A L F G T K D T T T A H S D Y E I I L E G G S S S W G Q V K G R A K V N - V P A A L P L L P A D C N I K I E A K P L D 

M A L F G T K D T T T A H S D Y E I I L E G G S S S W G K V K G R A K V N N V P P A S P L L P A D C N V K I N V K P L D 


Chl. tepidum FmoA 


Chl. phaeobacteroides FmoA 

Ptc. aestuarii FmoA 


























70 80 90 100 110 120 

I E D D V V R L T Y F G Q S I V D G I L G R V E G E T D I A N E S P T R R V A A G E G K I T V G K F S H R F E F E G V V 

P A K G F V R I S A V F E S I V D S T K N K L T I E A D I A N E T K E R R I S V G E G M V S V G D F S H T F S F E G S V 

P A K G F V R F S A V I E S I V D S T K N K L V V E A D I A N E T K E R R I C V G E G S V S V G D F S H S F S F E G S V 

P A K G F V R F S A V I E S I V D S T K N K L V V E A D I A N E T K E R R I C V G E G S V T V G D F S H S F S F E G S V 

T Q K G V V R F T S T I E S I V D S T K N K L V V E V D I A N E T K D R R V A V G E G E V T V G D F S H K F S F E G S V 

P A K G F V R F S A V I E S I V D S T K N K L V V E A D I A N E T K E R R I V G E G V T V G D F S H . F S F E G S V 

130 140 150 160 170 180 

D C L R Y W R S K A I D D N I V A R K Q R L L C G N L F H D L S V R V P L D S E E V I D T W L E M Q D A F R N - S P N F 

V N L F Y Y R S D A V R R N V P N - - P I Y M Q G R Q F H D I L M K V P L D N N D L I D T W E G T V K A I G S - T G A F 

V N M Y Y Y R S D A V R R N V P N - - P I Y M Q G R Q F H D I I M K V P L D N P D V I D T W E G T V K A V Q S - T G A F 

V N L F Y Y R S D A V R R N V P N - - P I Y M Q G R Q F H D I I M K V P L D N N D V I D T W E G T V K A L Q S - T G S F 

V N M Y Y F R S D A V R R N V P N - - P I Y M Q G R Q F H D I M M K V P L D N N D L I D T W E G F Q Q S I S G G G A N F 

V N L . Y Y R S D A V R R N V P N R K P I Y M Q G R Q F H D I . M K V P L D N N D V I D T W E G T V K A . S G T G F 

190 200 210 220 230 240 

G D Y I K D V W L I G P L W N A L E Q T G Q S L D N I D V Y Y F S E V E G - - G E K S R I D F R F A G G G T G I V D S I 

N D W I R D F W F I G P A F T A L N E G G Q R I S R I E V N G L N T E S G P K G P V G V S R W R F S H G G S G M V D S I 

N D W I R D F W F I G P A F T A L N E G G Q R I S R I E V N S I G T Q G S D K G P V G V S R W R F S H G G S G V V D S I 

N D W I R E F W F I G P A F T A L N E G G Q R I S R I E V N S I G T Q S G E K G P V G V S R W R F S H G G S G I V D S I 

G D W I R E F W F I G P A F T A I N E G G Q R I S P I Q V N N F G V E S G E K G P V G V S R W K F S H A G S G I V D S I 

N D W I R D F W F I G P A F T A L N E G G Q R I S R I E V N . G T S G . K G P V G V S R W R F S H G G S G I V D S I 

250 260 270 280 290 300 

S R W L E L F P I D G L G K P V N Q G G R V A R L Q G N F N A S V Q G I E V K L F V E L P G F S V P I E G G K R K V L N 

S R W A E L F P S D K L N R P A Q - - - - - - - V E A G F R S D S Q G I E V K V D G E F P G V S V D A G G G L R R I L N 

S R W M E L F P S D K L N R P A S - - - - - - - V E A G F R S D S Q G I E V K V D G E F P G V S V D A G G G L R R I L N 

S R W A E L F P S D K L N K P A S - - - - - - - V E A G F R S D S Q G I E V K V D G E F P G V S V D A G G G L R R I L N 

S R W A E L F P V D Q L N K P A S - - - - - - - I E G G F R S D S Q G I E V K V D G N L P G V S R D A G G G L R R I L N 

S R W A E L F P S D K L N K P A S Q G G R V A R V E A G F R S D S Q G I E V K V D G E F P G V S V D A G G G L R R I L N 

310 320 330 340 350 360 

H P L V P L A H H G I A V N S E P A D L S I R F K V S I P K G K K F I E T A N S F E W D R V A E N V R V F T G G R Y K A 

H P L I P L V H H G M V G K F N N F N V D A Q L K V V L P K G Y K I R Y A A P Q Y R S Q N L E E Y R - - W S G G A Y A R 

H P L I P L V H H G M V G K F N D F T V D T Q L K I V L P K G Y K V R Y A A P Q F R S Q N L E E Y R - - W S G G A Y A R 

H P L I P L V H H G M V G K F N D F T V D T Q L K I V L P K G Y K V R Y A A P Q F R S Q N L E E Y R - - W S G G A Y A R 

H P L I P L V H H G M V G K F N D F T V D T Q L K V V L P K G Y K I R Y A A P Q F R S Q N L E E Y R - - W S G G A Y A R 

H P L I P L V H H G M V G K F N D F T V D T Q L K V V L P K G Y K . R Y A A P Q F R S Q N L E E Y R R V W S G G A Y A R 

370 380 390 400 410 420 

W A E G I C K G S Y S P F D I F F G 

W V E H V C K G G V G Q F E I L Y A Q 

W V E H V C K G G T G Q F E V L Y A Q 




Ligands to BChl a

Figure 6S 

A. 

B. bchG-orf-csmA bchY 

350,000 

0 50000 100000 150000 200000 250000 300000 350000 400000 

BAC M60-018 J19 

16S rRNA recA pscAB-fmoA 

BAC M60-018 J19 

450,000 550,000 650,000 750,000 850,000 950,000

Figure 7S 

GFP1 16S rRNA 

Cab. thermophilum 16S rRNA 

GFP1 16S rRNA 


GFP1 16S rRNA 


GFP1 16S rRNA 


GFP1 16S rRNA 


GFP1 16S rRNA 


GFP1 16S rRNA 


GFP1 16S rRNA 


GFP1 16S rRNA 


GFP1 16S rRNA 


GFP1 16S rRNA 


GFP1 16S rRNA 


GFP1 16S rRNA 


16S rRNA Alignment 

10 20 30 40 50 60 70 80 90 100 110 120 

T C G A A C G G G A A A G T C C T T C G G G A C G A G T A C A G T G G C G C A C G G G T G A G T A A C G C G T G G G T T A T C T A 

G A G A G T T T G A T C C T G G C T C A G A A T C A A C G C T G G C G G C A T G C C T A A C A C A T G C A A G T C G A A C G G G A A A G T C C T T C G G G A C G A G T A C A G T G G C G C A C G G G T G A G T A A C G C G T G G G T T A T C T A 

G A G A G T T T G A T C C T G G C T C A G A A T C A A C G C T G G C G G C A T G C C T A A C A C A T G C A A G T C G A A C G G G A A A G T C C T T C G G G A C G A G T A C A G T G G C G C A C G G G T G A G T A A C G C G T G G G T T A T C T A 

130 140 150 160 170 180 190 200 210 220 230 240 

C C T C C G G G T G G G G A A T A A C T C G G G G A A A C T C G A G C T A A T A C C G C A T A A T A C G T - C T A C G T T A A A G C A G C A A T G C G C C T G G A G A T G A G C C T G C G T C C G A T T A G C T A G T T G G T G G G G T A A T G 

C C T C C G G G T G G G G A A T A A C T C G G G G A A A C T C G A G C T A A T A C C G C A T A A T A C G T T C T A C G T T A A A G C A G C A A T G C G C C T G G A G A T G A G C C T G C G T C C G A T T A G C T A G T T G G T G G G G T A A T G 

C C T C C G G G T G G G G A A T A A C T C G G G G A A A C T C G A G C T A A T A C C G C A T A A T A C G T T C T A C G T T A A A G C A G C A A T G C G C C T G G A G A T G A G C C T G C G T C C G A T T A G C T A G T T G G T G G G G T A A T G 

250 260 270 280 290 300 310 320 330 340 350 360 

G C C C A C C A A G G C G A C G A T C G G T A G C C G G C C T G A G A G G G C G G T C G G C C A C A C T G G C A C T G A A A C A C G G G C C A G A C T C C T A C G G G A G G C A G C A G T G A G G A A T C T T G G G C A A T G G G C G A A A G C 



370 380 390 400 410 420 430 440 450 460 470 480 

C T G A C C C A G C A A T G C C G C G T G A A C G A T G A A G T C C T T C G G G A T G T A A A G T T C A T A A G C A G G A G A A G A A C A C C A T G A C G G T A T C C T G T G T A A G C C C C G G C C A A C T A C G T G C C A G C A G C C G C G 



490 500 510 520 530 540 550 560 570 580 590 600 

G T A A T A C G T A G G G G G C A A G C G T T G T T C G G A T T T A C T G G G C G T A A A G G G C G C G T A G G C G G C G C G T A A A G T C G G A C A T G A A A T C A T T A G G C T C A A C C T A A T G G C T G T G T C C G A T A C T T A C G C 

G T A A T A C G T A G G G G G C A A G C G T T G T T C G G A T T T A C T G G G C G T A A A G G G C G C G T A G G C G G C G C G T A A A G T C G G A C A T G A A A T C A T T A G G C T C A A C C T G A T G G C T G T G T C C G A T A C T T A C G T 

G T A A T A C G T A G G G G G C A A G C G T T G T T C G G A T T T A C T G G G C G T A A A G G G C G C G T A G G C G G C G C G T A A A G T C G G A C A T G A A A T C A T T A G G C T C A A C C T R A T G G C T G T G T C C G A T A C T T A C G Y 

610 620 630 640 650 660 670 680 690 700 710 720 

G C T T G G A G T G C G G G A G A G G C A G C C G G A A T T C T C G G T G T A G C G G T G A A A T G C G T A G A T A T C G A G A G G A A C A C C G G T G A C G A A G G T G G G T T G C T G G A C C G A C A C T G A C G C T G A G G C G C G A A A 



730 740 750 760 770 780 790 800 810 820 830 840 

G C T A G G G G A G C A A A C G G G A T T A G A T A C C C C G G T A G T C C T A G C C C T A A A C G A T G A A T A C T T G A T G T G C G A G T T A C T A G G C A C T C G C G T G T C G A A G C T A A C G C G A T A A G T A T T C C G C C T G G G 



850 860 870 880 890 900 910 920 930 940 950 960 

G A G T A T G A C C G C A A G G T T G A A A C T C A A A G G A A T N G A C G G G G A C C C G C A C A A G C G G T G G A G C A T G T G G T T T A A T T C G A C G C A A C G C G A A G A A C C T T A C C T G A G T T C A A G A T A C G A T G A A G C 

G A G T A T G A C C G C A A G G T T G A A A C T C A A A G G A A T T G A C G G G G A C C C G C A C A A G C G G T G G A G C A T G T G G T T T A A T T C G A C G C A A C G C G A A G A A C C T T A C C T G A G T T C A A G A T A C G A T G A A G C 

G A G T A T G A C C G C A A G G T T G A A A C T C A A A G G A A T T G A C G G G G A C C C G C A C A A G C G G T G G A G C A T G T G G T T T A A T T C G A C G C A A C G C G A A G A A C C T T A C C T G A G T T C A A G A T A C G A T G A A G C 

970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 

G C C G G A A T C G G C G T G G C C G G G C A A C C G G A G T C G T A T C A G G T G C T G C A T G G C T G T C G T C A G C T C G T G T C G T G A G A T G T T G G G T T A A G T C C C G C A A C G A G C G C A A C C C C T G T C G C C A G T T G C 



1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 

C A C C A G T T C G G C T G A G C A C T C T G G C G A G A C C G C T G A T G A T G A A T C A G A G G A A G G T G G G G A T G A C G T C A A G T C C T C A T G G C C C T T A T G C T C A G G G C T A C A C A C G T G C T A C A A T G G T G A A T A 



1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320 

A C A A A G A G A T G C A A A C C C G C G A G G G G G A G C G A A T C T C A A A A A T T T C A C C T C A G T T C G G A T T G C A G T C T G C A A C T C G A C T G C A T G A A G T C G G A A T C G C T A G T A A T C G C A G A T C A G C A C G C T 



1330 1340 1350 1360 1370 1380 1390 1400 1410 1420 1430 1440 

G C G G T G A A T A C G T T C C C G G G T C T T G T A C A C A C C G C C C G T C A C A T C A C G A A A G T A G G C T G C A C T A G A A G T G G C T G A G C T A A C C C G 

G C G G T G A A T A C G T T C C C G G G T C T T G T A C A C A C C G C C C G T C A C A T C A C G A A A G T A G G C T G C A C T A G A A G T G G C T G A G C T A A C C C G C A A G G G A G G C A G G T T A C A A C G G T G T G G T C T A T G A T T 

G C G G T G A A T A C G T T C C C G G G T C T T G T A C A C A C C G C C C G T C A C A T C A C G A A A G T A G G C T G C A C T A G A A G T G G C T G A G C T A A C C C G C A A G G G A G G C A G G T T A C A A C G G T G T G G T C T A T G A T T 

1450 1460 1470 1480 1490 1500 1510 1520 1530 1540 1550 1560 

G G G G T G A A G T C G T A A C A A G G T A G C T G T A G G A G A A C C T G T G G C T G G A T C A C C T C C T T 

G G G G T G A A G T C G T A A C A A G G T A G C T G T A G G A G A A C C T G T G G C T G G A T C A C C T C C T T

Figure 8S 

99 

94 

75 

77 

95 

83 

81 

70 

100 

99 

73 

99 

96 

92 

99 

99 

79 

96 99 

99 

97 

99 

92 

99 

84 

91 

71 

99 

70 

77 

99 

99 

80 

95 

99 

88 

99 

70 

73 

100 

96 

89 

99 

99 

99 

99 

100 

99 

Deinococcus radiodurans DR1105 

Dehalococcoides ethenogenes 

Herpetosiphon aurantiacus 

Chloroflexus aurantiacus J-10-fl 

Roseiflexus sp. RS1 

Roseiflexus castenholzii 

Thermoanaerobacter tengcongensis 

Thermoanaerobacter ethanolicus 

Clostridium novyi 

Clostridium botulinum 

Clostridium acetobutylicum 

Pelotomaculum thermopropionicum 

Carboxydothermus hydrogenoformans 

Heliobacterium modesticaldum 

Desulfitobacterium hafniense 

Streptococcus pyogenes 

Staphylococcus aureus 

Listeria monocytogenes 

Bacillus subtilis 

Bacillus cereus 

Bacillus anthracis 

Aquifex pyrophilus 

Aquifex aeolicus 

Deinococcus radiodurans 

Thermus thermophilus 

Thermus aquaticus 

Corynebacterium glutamicum 

Brevibacterium linens 

Arthrobacter aurescens 

Streptomyces coelicolor 

Nocardia farcinica 

Erythrobacter litoralis 

Blastochloris viridis 

Rhodopseudomonas palustris 

Bradyrhizobium japonicum 

Roseobacter denitrificans 

Rhodobacter sphaeroides 

Rhodobacter capsulatus 

Shewanella oneidensis 

Proteus vulgaris 

Shigella flexneri 

Escherichia coli 

Halorhodospira halophila 

Dechloromonas aromatica 

Bordetella pertussis 

Bordetella parapertussis 

Stigmatella aurantiaca 

Anaeromyxobacter sp. 

Anaeromyxobacter dehalogens 

Geobacter lovleyi 

Geobacter sulfurreducens 

Geobacter metallireducens 

Chloracidobacterium thermophilum 

Solibacter usitatus Ellin6076 

Acidobacteria sp. Ellin345 

Campylobacter jejuni 

Thiomicrospira denitrificans 

Wolinella succinogenes 

Helicobacter pylori 

Chloroherpeton thalassium 

Chlorobium ferooxidans 

Pelodictyon luteolum DSM 273 

Chlorobium phaeovibriodes DSM 265 

Chlorobium chlorochromatii 

Pelodictyon phaeoclathratiforme 

Prosthecochloris aesturarii DSM 271 

Chlorobium phaeobacteroides DSM 266 

Chlorobium limicola DSM 245 

Chlorobium phaeobacteroides BS1 

Chlorobium tepidum 

Chlorobaculum parvum DSM 263 

Cytophaga hutchinsonii 

Porphyromonas gingivalis 

Bacteriodes thetaiotaomicron 

Bacteriodes fragilis 

Petrotoga mobilis 

Thermotoga maritima 

Thermosipho melanesiensis 

Fervidobacterium nodosum 

Gloeobacter violaceus PCC 7421 

Synechococcus sp. 2-3B’a(2-13) 

Synechococcus sp. JA-3-3Ab 

Synechocystis sp. PCC 6803 

Synechococcus sp. PCC 7002 

Synechococcus sp. PCC 6301 

Nostoc sp. PCC 7120 

Nostoc punctiforme PCC 73102 

Chloroflexi 

Firmicutes (“Clostridia”) 

Firmicutes (“Bacilli”) 

Aquificae 

Deinococcus-Thermus 

Actinobacteria 

α-Proteobacteria 

γ-Proteobacteria 

β-Proteobacteria 

δ-Proteobacteria 

Acidobacteria 

ε-Proteobacteria 

Chlorobi 

Bacteriodetes 

Thermotogae 

Cyanobacteria

Cab. thermophilum RecA 

Solibacter usitatus RecA 

Acidobacteria sp. Ellin345 RecA 

Dehalococcoides ethenogenes RecA 

Herpetosiphon aurantiacus RecA 

Chloroflexus aggregans RecA 

Cfx. aurantiacus J-10-fl RecA 

Roseiflexus sp. RS1 RecA 

Roseiflexus castenholzii RecA 

The. tengcongensis RecA 

The. ethanolicus RecA 

Clostridium novyi RecA 

Clostridium botulinum RecA 

Clostridium acetobutylicum RecA 

Pel. thermopropionicum RecA 

Car. hydrogenoformans RecA 

Hb. modesticaldum RecA 

Desulfitobacterium hafniense RecA 

Streptococcus pyogenes RecA 

Staphylococcus aureus RecA 

Listeria monocytogenes RecA 

Bacillus subtilis RecA 

Bacillus cereus RecA 

Bacillus anthracis RecA 

Aquifex pyrophilus RecA 

Aquifex aeolicus RecA 

Deinococcus radiodurans RecA 

Thermus thermophilus RecA 

Thermus aquaticus RecA 

Corynebacterium glutamicum RecA 

Brevibacterium linens RecA 

Arthrobacter aurescens RecA 

Streptomyces coelicolor RecA 

Nocardia farcinica RecA 

Erythrobacter litoralis RecA 

Blastochloris viridis RecA 

Rhodopseudomonas palustris RecA 

Bradyrhizobium japonicum RecA 

Roseobacter denitrificans RecA 

Rhodobacter sphaeroides RecA 

Rhodobacter capsulatus RecA 

Shewanella oneidensis RecA 

Proteus vulgaris RecA 

Shigella flexneri RecA 

Escherichia coli RecA 

Halorhodospira halophila RecA 

Dechloromonas aromatica RecA 

Bordetella pertussis RecA 

Bordetella parapertussis RecA 

Stigmatella aurantiaca RecA 

Anaeromyxobacter sp. RecA 

Anaeromyxobacter dehalogens RecA 

Geobacter lovleyi RecA 

Geobacter sulfurreducens RecA 

Geobacter metallireducens RecA 

Campylobacter jejuni RecA 

Thiomicrospira denitrificans RecA 

Wolinella succinogenes RecA 

Helicobacter pylori RecA 

Chloroherpeton thalassium RecA 

Chlorobium ferooxidans RecA 

Pelodictyon luteolum RecA 

Chlorobium vibrioforme RecA 

Chlorobium chlorochromatii RecA 

Pld. .phaeoclathratiforme RecA 

Prosthecochloris aesturarii RecA 

Chlorobium phaeobacteroides RecA 

Chlorobium limicola RecA 


Chlorobium tepidum RecA 

Chlorobaculum parvum RecA 

Cytophaga hutchinsonii RecA 

Porphyromonas gingivalis RecA 

Bacteriodes thetaiotaomicron RecA 

Bacteriodes fragilis RecA 

Petrotoga mobilis RecA 

Thermotoga maritima RecA 

Thermosipho melanesiensis RecA 

Fervidobacterium nodosum RecA 

Gloeobacter violaceus RecA 

Synechococcus sp. JA-2-3B'a(2-13) 

Synechococcus sp. JA-3-3Ab RecA 

Synechocystis sp. PCC 6803 RecA 

Synechococcus sp. PCC 7002 RecA 


Nostoc sp. PCC 7120 RecA 

Nostoc punctiforme PCC 73102 RecA 


1 60 

1 59 

1 61 

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M T D T R Q E R N K A I D A A I A S L E K Q F G K G V V M R L G E R - - - - - - - - - - - Q A L E V P A I S T T C L S L D A A I G I G G V P R 

M D E K E R S R A L E T A L G Q I E K Q F G K G S I L R L G S K D - - - - - - - - - - A I V P V S V I S T G S I S L D A A L G V G G F P R 

M S D E R T E R G R S L E L A L S Q I E K Q F G K G S I M K L G A K G - - - - - - - - - - A I V P I S V I S T G A I S F D A A L G T G G F P R 

M T T E K D K A L E L T V G I I E K R F G K G S I M K L S D P T F - - - - - - - - - - R Q T V E F I P T S S L A L D I A L G V G G I P R 

M A I S P E K E K A L A A A L S Q I D R Q F G K G A I M R M S D Q - - - - - - - - - - - - R L S I E A I P T G S I A L D L A L G V G G I P R 

M A I T P E K E K A L A A T M A Q I D R K F G K G S I M K M G E A S S - - - - - - - - - - R V A I E A I P T G S I A L D I A L G I G G V P R 

M A L S P E K E K A L A A A M S Q I D R K Y G K G S I M R M G E V S S - - - - - - - - - - K L A I E V I P T G S I A L D I A L G V G G V P R 

M A L S P E K E K A L A A A M S Q I D R K Y G K G S I M R M G E A S S - - - - - - - - - - K L A I E V I P T G S I A L D I A L G V G G V P R 

M M I E K Q K A L E M A I S Q I E R Q F G K G - A I M R L G D T A - - - - - - - - - - K L N V E V I P T G S L E L D I A L G V G G V P R 

M I E K Q K A L E M A I S Q I E R Q F G K G - S I M R L G D T A - - - - - - - - - - K L N V E V I P T G S L E L D I A L G V G G V P R 

M N N E K M K A L Q A A I T Q I E K Q F G K G - S V M K L G E E H - - - - - - - - - - V L N V E A I S T G S L S L D I A L G I G G I P R 

M A N K V N P E K L K A I E A A M G Q I E K Q F G K G - S I M K L G E D S - - - - - - - - - - I L N V E S I S T G S L D L D I A L G I G G V P R 

M D N E K L K A I D A A M S Q I E K Q F G K G - A I M K L G E Q D - - - - - - - - - - I L N I D A I S T G C L S L D I A L G I G G V P R 

M S D R Q K A L E L A L M Q I E R Q F G K G S I M K L G E A S A - - - - - - - - - - K L N V E V I P T G A L A L D I A L G V G G V P R 

M N D K L K A L E L T I S Q I E K Q F G K G S I M R L G E N T G - - - - - - - - - - K F Q V E V I P T G S L A L D L A L G V G G V P R 

D N K L Q A L Q Q A L N S I E K A F G K G S I M R L G E A S A - - - - - - - - - - K L L V E V I P T G S I A L D M A L G I G G V P R 

M A G N D K L K A L D M A L S Q I E K Q F G K G S I M R L G E A S A - - - - - - - - - - K M A V E V I P T G C L A I D I A L G V G G V P R 

M A K K L K K N E E I T K K F G D E R R K A L D D A L K N I E K D F G K G A V M R L G E R A E - - - - - - - - - - - Q K V Q V M S S G S L A L D I A L G A G G Y P K 

M D N D R Q K A L D T V I K N M E K S F G K G A V M K L G D N I G - - - - - - - - - - - R R V S T T S T G S V T L D N A L G V G G Y P K 

M N D R Q A A L D Q A L K Q I E K Q F G K G S I M K L G E H S D - - - - - - - - - - - Q N I S T I S S G S L A L D I A L G V G G Y P R 

M S D R Q A A L D M A L K Q I E K Q F G K G S I M K L G E K T D - - - - - - - - - - - T R I S T V P S G S L A L D T A L G I G G Y P R 

M S D R Q A A L D M A L K Q I E K Q F G K G S I M K L G E Q A E - - - - - - - - - - - R K V S T V S S G S L A L D V A L G V G G Y P R 

M S D R Q A A L D M A L K Q I E K Q F G K G S I M K L G E Q A E - - - - - - - - - - - R K V S T V S S G S L A L D V A L G V G G Y P R 

M A R V S E N L S E K M K A L E V A L S S I E K R F G K G A V M P L K A V E T - - - - - - - - - - - V E V E T I P T G S I S L D I A T G V G G I P K 

M S E N L S E K K K A L E V A L S N I E K R F G K G A V M P L K S V E K - - - - - - - - - - - V Q V E T I P T G S L A L D I A T G V G G I P K 

M S K D A T K E I S A P T D A K E R S K A I E T A M S Q I E K A F G K G S I M K L G A E S K - - - - - - - - - - - L D V Q V V S T G S L S L D L A L G V G G I P R 

M D E S K R K A L E N A L K A I E K E F G K G A V M R L G E M P K - - - - - - - - - - - Q Q V D V I P T G S L A L D L A L G I G G I P R 

M D E S K R K A L E N A L K A I E K E F G K G A V M R L G E M P K - - - - - - - - - - - Q Q V D V I P T G S L A L D L A L G I G G I P R 

M A P K K T A T K A T A A K G N D R Q K A L D A A L A L I E K D F G K G A V M R L G - D E N - - - - - - - - - - R P P I Q T I S S G N T A I D I A L G I G G F P R 

M A R T P K N L Q I P T G G D K S K A L D A A L G Q I D R N Y G K G A I M R L G - E G V - - - - - - - - - - R E P I A C I P T G S I A L D I A L G I G G L P R 

M A A T P D R E K A L E A A L A Q I D K Q F G K G S I M R L G - D D T - - - - - - - - - - R A P I E V I P T G S I A L D V A L G I G G L P R 

M A G T D R E K A L D A A L A Q I E R Q F G K G A V M R M G - D R T - - - - - - - - - - N E P I E V I P T G S T A L D V A L G V G G I P R 

M A P Q A Y D R D K A L E L A L A Q V E K S F G K G A V M R L G - E E A - - - - - - - - - - R Q P I S V I P T G S I A L D V A L G I G G L P R 

M S A N L K L V E K E N N V D R Q K A L D A A L A Q I D R A F G K G S A M K L G S K E - - - - - - - - - - - A M N V E A I S T G S L G L D I A L G I G G L P K 

M D K T K A L D A A L T Q I E R A F G K G S I M R L G K S G A - - - - - - - - - - A L E V E T I S T G S L G L D I A L G I G G L P R 

M A A P T A L R I V E G S S M D K S K A L S A A L S Q I E R Q F G K G S V M R L G K N D R - - - - - - - - - - S M D I E T I S S G S L G L D I A L G V G G L P K 

M S A T A L R I V E G S S M D K S K A L A A A L S Q I E R Q F G K G S V M K L G K N D R - - - - - - - - - - S M D V E A V S S G S L G L D I A L G I G G L P K 

M A T A D L L S M N S K K S A D K Q K A L D S A L A Q I E R Q F G K G S I M K L G A E G A - - - - - - - - - - V Q D I Q A S S T G S L G L D I A L G I G G L P M 

M A T A N L L D L N G R R E M D K A K A L E S A L A Q I E R Q F G K G S I M K L G A N S P - - - - - - - - - - V M E I E A T S T G S L G L D I A L G I G G L P K 

M A T T G L F E M N D K G K A D K Q K A L E C A L A Q I E R Q F G K G S I M K L G G D T P - - - - - - - - - - P P E I E A T S T G S L G L D I A L G I G G L P K 

M K V D P N K E K A L A A V L S Q I E K Q F G K G S I M K L G E D - - - - - - - - - - - R S M D V E T I S T G S L S L D V A L G A G G L P M 

M A I D E N K Q K A L A A A L G Q I E K Q F G K G S I M R L G E D - - - - - - - - - - - R S M D V E T I S T G S L S L D I A L G A G G L P M 



M D D D R K K A L G A A L G Q I E K Q F G K G A V M R M G D A R - A - - - - - - - - V R G D V K S I S T G S L A L D V A L G I G G L P R 

M D D N K A K A L A A A L Q Q I E K Q F G K G S I M K M G D A - - E - - - - - - - - I D E G I Q V V S T G S L G L D I A L G V G G L P R 

M D D K T S K A A A A E K A K A L A A A L S Q I E K Q F G K G S I M R Y G D N - - E - - - - - - - - V E H D I Q V V S T G S L G L D I A L G V G G L P R 

M D D K T S K A A A A E K A K A L A A A L S Q I E K Q F G K G S I M R Y G D N - - E - - - - - - - - V E H D I Q V V S T G S L G L D I A L G V G G L P R 

M A V N S E K E K A I E L A M S A V E R Q F G K G S I M R L G K D E P - - - - - - - - - L T R D I Q A I S T G S I S L D I A L G V G G V P K 

M A V A P E K E K A I E L A V S S I E K A F G K G S I M R L G N E E A - - - - - - - - - L V K D V Q A V S T G S V S L D I A L G V G G F P R 

M P V G P E K E K A I E L A V A S I E K A F G K G S I M R L G N E D A - - - - - - - - - L V K D V Q A V S T G A I S L D I A L G V G G F P R 

M S D K N K A I E L A L S Q I E K Q F G K G A I M R L G N D E - - - - - - - - - - V L P G V E S I S T G A I S L D L A L G V G G V P R 

M T Q E R E K A I E L A L S Q I E K Q F G K G A I M R L G A D E - - - - - - - - - - A L P D V A A I P T G S L S L D L A L G V G G V P R 

M T Q E R E K A I E L A L S Q I E K Q F G K G A I M R L G A D E - - - - - - - - - - A L P D I D A I P T G A L S L D I A L G V G G V P R 

M D D N K R K S L D A A L K S L D K T F G K G T I L R L G - D K E - - - - - - - - - - V E Q I D S I G T G S V G L D L A L G I G G V P K 

M D A N K Q K S L D L A M K Q I D K A F G K G A L M R L G - D K E - - - - - - - - - - I M P I E S I S T G S L G L D L A L G I G G I P Q 

M A L D E N K R K A I D L A I K Q I D K A F G K G A L V R L G - E K P - - - - - - - - - - V E K I D S I S T G S L G L D I A L G I G G I P Q 

M A I D E D K Q K A I S L A I K Q I D K V F G K G A L V R L G - D K Q - - - - - - - - - - V E K I D S I S T G S L G L D L A L G I G G V P K 

M A K E K A S S Q E Q E Q K V K D E K L K Q L N L A V E S L E K Q F G K G A I M R L G - D D A - - - - - - - - - - V V Q V P V V S T G S I S L D Y A L G V G G L P K 

M D T Q K I E Q S V P P V V D P A K L K Q L N L A V D A L E K Q F G K G A I M R L G D S S A - - - - - - - - - - V M Q V L S I S T G S M T L D F A L G V G G L P R 

M A T P R P A E T A P G V D P A K L K Q L N L A V E A L E K Q F G K G A I M R L G D D S A - - - - - - - - - - V M Q V Q S I S T G S M T L D F A L G V G G L P R 

M D T T S K K E K K V P G I D P A K L K Q L D L A V D S L E K Q F G K G A I M R L G D D S A - - - - - - - - - - V M Q V Q A I S T G S M T L D F A L G V G G L P R 

M T M D N P K V E Q A G H A V D S A K L K Q L N L A V D A L E K Q F G K G T I M R M G D G S A - - - - - - - - - - G L T V Q A I S T G S M A L D F A L G V G G L P R 

M D T Q K I E K K T V T V D P A K L K Q L N L A V E A L E K Q F G K G A I M R L G D D S A - - - - - - - - - - G M K V Q S I S T G S M T L D F A L G V G G L P R 

M D T Q K I E K K T V T V D P A K L K Q L N L A V E A L E K Q F G K G A I M R L G D D S A - - - - - - - - - - G M K V Q S I S T G S M T L D F A L G V G G L P R 

M D N I K T E Q K S D L V D P A K L K Q L N L A V D A I E K Q F G K G A I M R L G D D S A - - - - - - - - - - G M H V Q A I S T G S M T L D F A L G V G G L P R 

M D N I K T E Q K S D L V D P A K L K Q L N L A V D A I E K Q F G K G A I M R L G D D S A - - - - - - - - - - G M H V Q A I S T G S M T L D F A L G V G G L P R 

M D T K K N V Q E K K T A D P A K L K Q L S L A V E T L E K Q F G K G A I M C L G D Q G A - - - - - - - - - - I I P V Q S V S T G S I A L D F A L G V G G F P R 

M E K E A T P Q Q Q P P V V D P A R L K Q L N L A I E T L E K Q F G K G A I M R L G D D S A - - - - - - - - - - V M H V Q V I S T G S M A L D Y A L G V G G L P R 

M D K E N S K Q T A Q P P V V D P A R L K Q L N L A I E T L E K Q F G K G S I M R L G D D S A - - - - - - - - - - V M H V K A I S T G S M A L D Y A L G V G G F P R 

M S K S T S T P N P Q N E K L K A L Q L T I D K L E K T Y G K G T V M K L S - D E V - - - - - - - - - - V M D V P V I S T G S L G L D I A L G I G G L P K 

M A E E K I P T V Q D E K K L Q A L R M A T E K I E K T F G K G A I M N M G - A N T - - - - - - - - - - V E D V S V I P S G S I G L D L A L G V G G Y P R 

M A K K D E L N F E T D N - K M A S S E K L K A L Q A T M E K I E K N F G K G S I M K M G - D D S - - - - - - - - - - V E Q V E V I P T G S I A L N V A L G V G G Y P R 

M A K K E D E L N F E T D N N K M A S S E K L K A L Q A A M D K I E K S F G K G S I M K M G - E E V - - - - - - - - - - V E Q V E V I P T G S I A L N A A L G V G G Y P R 

M A K N D S K D A N K E E L L E K L V K E L E K N H G T G S V M L M G K G L D - - - - - - - - - - N S N L A V V P S G C L S L D I A L G V G G Y P R 

M P E E K Q K K S V L E K A L K R I E E N F G K G S I M I L G D E T Q - - - - - - - - - - V Q P V E V I P T G S L A I D I A T G V G G Y P R 

M S D N K Q K K E V L K K A I G K I E K T F G K G S I M F L G E E N A - - - - - - - - - - V Q N I E T I S S G S V A I D I A S G V G G Y P R 

M S D E M K K D V L K K A I S K I E K T Y G K G S I M V L G E G N L - - - - - - - - - - V Q E I D V I P S G S L A I D I A S G V G G Y P R 

M A R T T D D S K K A A P A A G T A D E A Q K Q K A L K M V L T Q I K R N F G E G A I M R L G - E N T - - - - - - - - - - R I R V E T V P S G A I T L D L A L G G G L P - R 

M K S E K R K A L E Q V L S Q I E R N F G K G A I M R L G - D A T - - - - - - - - - - Q M R V E T I P S G A I T L D L A L G G G Y P - K 

M K S E K R K A L D Q V L G Q I E R N F G K G A I M R L G - D A A - - - - - - - - - - Q M R V E T I P S G A I T L D L A M G G G Y P - K 

M A S T N I S D R E K A L N A A L A Q I E R S F G K G A I M R L G - D A T - - - - - - - - - - Q M R V E T I S T G A L T L D L A L G G G L P - K 

M S A I S N N P D K E K A L N L V L N Q I E R N F G K G A I M R L G - D A A - - - - - - - - - - Q M K V A T I P S G A L T L D Q A M G G G F P - R 

M A S K S D A I A P E K E K A L N L V L S Q I E R N F G K G A I M R L G - D A A - - - - - - - - - - R L R V E T I S T G A L T L D L A L G G G L P - K 

M A I N T D T S G K Q K A L T M V L N Q I E R S F G K G A I M R L G - D A T - - - - - - - - - - R M R V E T I S T G A L T L D L A L G G G L P - K 

M A I N T D T S G K Q K A L T M V L N Q I E R S F G K G A I M R L G - D A T - - - - - - - - - - R M R V E T I S S G A L T L D L A L G G G L P - K 

M E R V A V L F R R T C N S E N C S K G R R P L R E R P L A G L F F C S L P A P L S I P C L S L P G S N P G D F V P K V K T N Y I C N S C G Y Q S A K P L G R C P N C Q A W N S F E E E V P T A S T S G K S G R G G L G G Y G G V K G G K L T P L S T V G R R E E P R T P S G I P E L D R V L G G G L V - A 

































































Pld. phaeoclathratiforme RecA 
























61 170 

60 169 

62 171 

59 168 

59 168 

1 108 

61 170 

61 170 

61 170 

58 167 

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62 171 

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61 170 

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57 166 

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61 170 

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150 299 

G R I V E I Y G P E S G G K T T L A L H V A A E A Q R T G G N V A F I D A E H A L D P G Y A A K L G V D I D N L F V S Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E A L V R S A A F D L V V V D S V A A L V P K A E L D G - - - - - D M G D A L P G L Q A 

G R I N E I F G P E S S G K T T I A L Q V V A E A Q K A G G I A A F I D V E H A L D P T Y A R K L G V D V D N L L V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I T A H L I A S G Q I D V L V V D S V A A L V P K S E L D G - - - - - E M G D S H M G V Q A 

G R V V E I F G P E S S G K T T I A L Q V I A E A Q K A G G M A A F V D A E H A L D P T Y A R K L G V D V E N L L V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I T E A L V S S K A I D I L V V D S V A A L V P K A E L D G - - - - - E M G D S H M G L Q A 

G R I S E I F G P E G S G K T T L A Q H I I A Q A Q K M G E K A A Y I D V E H A L D P K Y A S T C G V N L D E L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - E A L G I A E E L V R S T A I G V I V I D S V A A L V P K A E I E G - - - - - D M G D S H V G L Q A 

G R V I E I Y G P E S S G K T T L T Q H I V A E T Q K M G G V A A F I D A E H A F D P V Y A A N C G V N V E D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E T L V R S G A I D V I V V D S V A A L V P R A E I E G - - - - - D M G D S H V G L Q A 

M I E I F G P E S S G K T T L A Q H I I A E A Q K M G G V G A F I D A E H A F D P V Y A A R C G V N I S D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E M L V R S N A V D V I V I D S V A A L V P R A E I E G - - - - - D M G D S M P G M Q A 

G R V V E I F G P E S S G K T T L A Q H I I A E A Q K M G G V C A F I D A E H A F D P V Y A A R C G V N I D D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E M L V R S N A I D V I V I D S V A A L V P R A E I E G - - - - - E M G D S M P G M Q A 

G R V V E I Y G P E S S G K T T L A Q H I I A E A Q K M G G I A A F I D A E H A F D P V Y A A R C G V D V N N L L V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E A L V R S N A V D V V V V D S V A A L V P R A E I E G - - - - - D M G D S L P G L Q A 

G R V V E I Y G P E S S G K T T L A Q H I I A E A Q K M G G V A A F I D A E H A F D P V Y A K R C G V D V D N L L V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E T L V R S N A V D V V V V D S V A A L V P R A E I E G - - - - - D M G D S L P G L Q A 

G R I I E I F G P E S S G K T T L A L H M I A E A Q K I G G T G A F I D A E H A L D P V Y A K N L G V N I D D L L V A Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E A L V R S G A V D I I V I D S V A A L V P K A E I E G - - - - - E M G D S H V G L Q A 

G R I I E I F G P E S S G K T T L A L H M I A E A Q K M G G T G A F I D A E H A L D P V Y A K N L G V N I E D L L V A Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E A L V R S G A V D I I V I D S V A A L V P K A E I D G - - - - - E M G D S H V G L Q A 

G R I I E I F G P E S S G K T T V A L H I I A E A Q K S G G T A A F I D A E H A L D P V Y A K A L G V D I D N L I V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E A L V R S G A I D V I V V D S V A A L V P K A E I E G - - - - - E M G D S H V G L Q A 

G R I I E I F G P E S S G K T T V A L H I L A E A Q K I G G A A A F I D A E H A L D P S Y A R N L G V D I D N L I V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I T E A L V R S G A V D V I V V D S V A A L V P R A E I E G - - - - - E M G D S H V G L Q A 

G R V V E I Y G P E S S G K T T V A L H I L A E A Q K K G G A A A F I D A E H A L D P Q Y A R A L G V D I D N L V V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E A L V R S G A I D V I V V D S V A A L V P K A E I E G - - - - - E M G D S H V G L Q A 

G R V I E I F G P E S S G K T T V A L H V I A E A Q K M G G T A A F V D A E H A L D P V Y A K N L G V D I E N L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E A L V R S G A V D V I V V D S V A A L V P R A E I E G - - - - - E M G D A H V G L Q A 

G R V V E I F G P E S S G K T T V A L H I I A E A Q K M G G I A A F I D A E H A L D P V Y A Q K L G V D I E N L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E S L V R S G A I D V I V I D S V A A L V P R A E I E G - - - - - E M G D A H V G L Q A 

G R I V E V Y G P E S S G K T T V A L H I I A E A Q K M G G M A A F I D A E H A L D P V Y A R N L G V D I D N L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E A L V R S G A I D V V V I D S V A A L V P K A E I D G - - - - - E M G D A H V G L Q A 

G R V I E I Y G P E S S G K T T V A L H I A A E A Q K L G G V A A F I D A E H A L D P V Y A K A L G V N I D N L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E A L V R S G A I D V I I I D S V A A L V P R A E I E G - - - - - E M G D S H V G L H A 

G R I I E I Y G P E S S G K T T V A L H A V A Q A Q K E G G I A A F I D A E H A L D P A Y A A A L G V N I D E L L L S Q P D S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q G L E I A G K L I D S G A V D L V V V D S V A A L V P R A E I D G - - - - - D I G D S H V G L Q A 

G R I I E I Y G P E S S G K T T V A L H A I A E V Q S N G G V A A F I D A E H A L D P E Y A Q A L G V D I D N L Y L S Q P D H - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q G L E I A E A F V R S G A V D I V V V D S V A A L T P K A E I E G - - - - - E M G D T H V G L Q A 

G R I I E V Y G P E S S G K T T V A L H A I A E V Q A Q G G T A A F I D A E H A L D P A Y A K N L G V N I D E L L L S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E A L V R S G A V D M L V I D S V A A L V P R A E I E G - - - - - E M G D A H V G L Q A 

G R I I E V Y G P E S S G K T T V A L H A I A E V Q Q Q R - T S A F I D A E H A L D P V Y A Q K L G V N I E E L L L S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E A L V R S G A V D I V V V D S V A A L V P K A E I E G - - - - - D M G D S H V G L Q A 

G R I I E I Y G P E S S G K T T V S L H A I A E V Q R Q G G Q A A F I D A E H A M D P V Y A Q K L G V N I D E L L L S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q G L E I A E A L V R S G A V D I I V I D S V A A L V P K A E I E G - - - - - D M G D S H V G L Q A 

G R I I E I Y G P E S S G K T T V S L H A I A E V Q R Q G G Q A A F I D A E H A M D P V Y A Q K L G V N I D E L L L S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q G L E I A E A L V R S G A V D I I V I D S V A A L V P K A E I E G - - - - - D M G D S H V G L Q A 

G R I T E I F G V E S S G K T T L A L H V I A E A Q K R G G V A V F I D A E H A L D P K Y A K K L G V D V D N L Y I S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E S L I N S G A V D V I V V D S V A A L V P K D E L E G - - - - - E M G E A Q V G K Q A 

G R I T E I F G V E S S G K T T L A L H V I A E A Q K R G G V A V F I D A E H A L D P K Y A K K L G V D V E N L Y I S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E S L I N S G A V D V I V V D S V A A L V P K D E L E G - - - - - E M G E A Q V G K Q A 

G R I T E I Y G P E S G G K T T L A L A I V A Q A Q K A G G T C A F I D A E H A L D P V Y A R A L G V N T D E L L V S Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I M E L L V R S G A I D V V V V D S V A A L T P R A E I E G - - - - - D M G D S L P G L Q A 

G R I V E I Y G P E S G G K T T L G L T I I A Q A Q R R G G V A A F V D A E H A L D P L Y A Q R L G V Q V E D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V E L L A R S G A V D V I V V D S V A A L V P R A E I E G - - - - - E M G D Q H V G L Q A 

G R I V E I Y G P E S G G K T T L A L T I I A Q A Q R R G G V A A F V D A E H A L D P L Y A Q R L G V Q V E D L L V S Q P E T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V E L L A R S G A V D V I V V D S V A A L V P R A E I E G - - - - - E M G D Q H V G L Q A 

G R I V E V Y G P E S S G K T T V A L H A I A Q A Q K A G G I A A F I D A E H A L D P D Y A R K L G V D T D A L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A D M L V R S G A I D I I V I D S V A A L T P K A E I E G - - - - - E M G D S H V G L Q A 

G R V V E I Y G P E S S G K T T V A L H A V A N A Q K T G G I A A F I D A E H A L D P E Y A K K L G V D T D Q L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A D M L I R S G A L D V I V I D S V A A L V P K A E I E G - - - - - E M G D S H V G L Q A 

G R V V E I Y G P E S S G K T T V A L H A V A N A Q R A G G I A A F I D A E H A L D P D Y A A K L G V D T D A L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I M D M L V G S G S L D I V V I D S V A A L V P R A E I E G - - - - - E M G D S H V G L Q A 

G R V V E V Y G P E S S G K T T L T L H A V A N A Q K A G G Q V A F V D A E H A L D P E Y A K K L G V D I D N L I L S Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V D M L V R S G A L D L I V I D S V A A L V P R A E I E G - - - - - E M G D S H V G L Q A 

G R I V E I Y G P E S S G K T T V A L H A V A N A Q A A G G V A A F I D A E H A L D P D Y A R K L G V D T D A L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A D M L V R S G A I D I I V I D S V A A L V P R A E I E G - - - - - E M G D S H V G L Q A 

G R V I E V Y G P E S S G K T T L A L H A I A E A Q K A G G T A A F V D A E H A L D P V Y A K A L G V D I D E L I V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I T D T L V R S N A V D I L V V D S V A A L V P R A E I E G - - - - - E M G D S H V G L Q A 

G R V V E I F G P E S S G K T T L A L H T V A E A Q K K G G V C A F I D A E H A L D P L Y A R K L G V N L D D L L I S Q P D A - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A D T L V R S G A I D V L V V D S V A A L V P R A E L E G - - - - - E M G D S Q P G M Q A 

G R I V E I Y G P E S S G K T T L A L H C V A E A Q K K G G I C A F V D A E H A L D P V Y A R K L G V N V D D L L I S Q P D H - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A D T L V R S G A I D V L I V D S V A A L V P R A E L E G - - - - - E M G D A L P G L Q A 

G R V V E I Y G P E S S G K T T L A L H T V A E A Q K K G G I C A F I D A E H A L D P V Y A R K L G V N I D E L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C D T L V R S G A V D V L V V D S V A A L V P K A E L E G - - - - - E M G D A L P G L Q A 

G R I V E I Y G P E S S G K T T L T L H C V A E Q Q K A G G V C A F V D A E H A L D P T Y A K K L G V D L D E L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I T D T L V R S G A V N M V I V D S V A A L T P K S E L E G - - - - - D M G D S S V G V Q A 

G R I I E I Y G P E S S G K T T L T L H V V A E E Q K K G G V C A F V D A E H A L D P Q Y A K K L G V N L D E L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V D T L V R S G A V N L I V V D S V A A L T P K S E I E G - - - - - D M G D M Q M G S Q A 

G R I V E I Y G P E S S G K T T L T L H C I A E E Q K K G G V C A F V D A E H A L D P Y Y A K K L G V S L E D L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V D T L V R S G A V S L V V V D S V A A L T P K A E I E G - - - - - D M G D A T V G A Q A 

G R I V E I Y G P E S S G K T T L T L E V I A A A Q R E G K T C A F I D A E H A L D P I Y A K K L G V D I D N L L C S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C D A L T R S G A V D V I I V D S V A A L T P K A E I E G - - - - - E I G D S H M G L A A 

G R I V E I Y G P E S S G K T T L T L Q V I A A A Q R E G K T C A F I D A E H A L D P I Y A K K L G V D I D N L L C S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C D A L T R S G A V D V I I V D S V A A L T P K A E I E G - - - - - E I G D S H M G L A A 

G R I V E I Y G P E S S G K T T L T L Q V I A A A Q R E G K T C A F I D A E H A L D P I Y A R K L G V D I D N L L C S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C D A L A R S G A V D V I V V D S V A A L T P K A E I E G - - - - - E I G D S H M G L A A 

G R I V E I Y G P E S S G K T T L T L Q V I A A A Q R E G K T C A F I D A E H A L D P I Y A R K L G V D I D N L L C S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C D A L A R S G A V D V I V V D S V A A L T P K A E I E G - - - - - E I G D S H M G L A A 

G R V V E I Y G P E S S G K T T L T L H I I A E A Q K A G G A A A F V D A E H A L D P D Y A E A V G V D I N D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A D M L V R S G G L D V V V V D S V A A L T P K A E I E G - - - - - E M G D S H V G L Q A 

G R V V E I Y G P E S S G K T T L C L Q V V A E M Q K L G G V A A F I D A E H A L D P Q Y A Q K L G V N V G D L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A D M L V R S G S V D I I V V D S V A A L T P R A E I E G - - - - - E M G D Q M V G L H A 

G R V I E V Y G P E S S G K T T L T L Q V I A E M Q K L G G T C A F V D A E H A L D V Q Y A S K L G V N L T D L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I T D A L V R S G S V D L I V I D S V A A L V P K A E I E G - - - - - E M G D S L P G L Q A 

G R V I E V Y G P E S S G K T T L T L Q V I A E M Q K L G G T C A F V D A E H A L D V Q Y A S K L G V N L T D L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I T D A L V R S G S V D L I V I D S V A A L V P K A E I E G - - - - - E M G D S L P G L Q A 

G R I V E I F G P E S S G K T T L C L H I V A E A Q K R G G I C G Y I D A E H A L D V G Y A R K L G V R T D D L L L S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E M L V R S G A I D V L V V D S V A A L V P K A E L E G - - - - - E M G D A H M G V Q A 

G R I I E I Y G P E S S G K T T L A L H A V A E A Q K K G G I A A F V D A E H A L D V G Y A R K L G V R T D D L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E T L V R S G A I D V L V V D S V A A L V P K A E L E G - - - - - E M G D A H M G V Q A 

G R I I E I Y G P E S S G K T T L A L H A I A E A Q R R G G I A A F V D A E H A L D V G Y A R K L G V R T D D L L I S Q P D S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V E T L V R S G A I D V L V V D S V A A L V P K A E L E G - - - - - E M G D A H M G V Q A 

G R V I E V Y G P E S S G K T T L A L H I V A E A Q K S G G I A A F V D A E H A L D I N Y A R K L G V K T D D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E T L V R S G A I D V L V I D S V A A L V P K A E I E G - - - - - E M G D S H V G L Q A 

G R I I E I Y G P E S S G K T T L A L H I A A E A Q K M G G I A A F V D A E H A L D I G Y A R K L G V K T D D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E M L V R S G A V D V L V I D S V A A L V P K A E I E G - - - - - E M G D S H M G L Q A 

G R V I E I Y G P E S S G K T T L A L H I A A E A Q K L G G I A A F V D A E H A L D I G Y A R K L G V R T D D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E M L V R S G A V D V L V I D S V A A L V P K A E I E G - - - - - E M G D S H V G L Q A 

G R I I E I Y G P E S S G K T T L T L H I I A E C Q K A G G V C A F I D A E H A L D V K Y A K N L G V N T D D L Y V S Q P D F - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V E T I A R S G A V D L I V V D S V A A L T P K A E I E G - - - - - D M G D Q H V G L Q A 

G R V V E I Y G P E S S G K T T L A L Q I T A E C Q K A G G V C A F I D A E H A L D V G Y A K N L G V D V D N L L V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L D I V E T I A R S G A I D L I V I D S V A A L T P K S E I E G - - - - - E M S D Q N V G V Q A 

G R I I E I Y G P E S S G K T T L A L Q V I A E C Q K K G G I C A F I D A E H A L D V S Y A K R L G V D A E N L L V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I L E T L T R S G A V D L I V V D S V A A L T P K S E I D G - - - - - D M G D Q H V G L Q A 

G R I I E I Y G P E S S G K T T L S L H I I A E C Q K N G G V C A F I D A E H A L D V H Y A K R L G V D T E N L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I L E T I T R S G G I D L V V V D S V A A L T P K A E I D G - - - - - D M G D Q H V G L Q A 

G R I V E I Y G P E S S G K T T L A L H A I A E A Q K A G G I A A F V D A E H A F D Q S Y A R K L G I D I K S L L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I T E T L V R S G A V D I I V V D S V A A L V P Q A E L E G - - - - - E M G D S Q M G L Q A 

G R V T E I Y G P E S S G K T T L A L H V I A E A Q K E G G I A A L V D A E H A F D P S Y A R R L G V D I N S M L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S G A V D V V V V D S V A A L V P Q A E L E G - - - - - E M G D S V V G L Q A 

G R V T E I Y G P E S S G K T T L A L H V I A E A Q K A G G I A A L V D A E H A F D P S Y A R K L G V D I N A M L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S G A V D V V V V D S V A A L V P Q A E L E G - - - - - E M G D S V V G L Q A 

G R V T E I Y G P E S S G K T T L A L H V I A E A Q K A G G I A A I V D A E H A F D P S Y A R R L G V D I N A L L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S G A V D V V A V D S V A A L V P Q A E L E G - - - - - E M G D T V V G L Q A 

G R V T E I Y G P E S S G K T T L A L H V I A E A Q K E G G I T A I V D A E H A F D P S Y A R K L G V D I N A L L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S G A V D V V V V D S V A A L V P Q A E L E G - - - - - E M G D S S M G L Q A 

G R V T E I Y G P E S S G K T T L A L H V I A E A Q K E G G I A A I V D A E H A F D P T Y A R K L G V D I N A L L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S N A I D V V V V D S V A A L V P Q A E L E G - - - - - E M G D S V M G L Q A 

G R V T E I Y G P E S S G K T T L A L H V I A E A Q K E G G I A A I V D A E H A F D P T Y A R K L G V D I N A L L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S N A I D V V V V D S V A A L V P Q A E L E G - - - - - E M G D S V M G L Q A 

G R V T E I Y G P E S S G K T T L A L H V I A E A Q K E G G I A A I V D A E H A F D P T Y A R K L G V D I N A L L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L T I V E T L V R S G A V D V V V V D S V A A L V P Q A E L E G - - - - - E M G D S V M G L Q A 

G R V T E I Y G P E S S G K T T L A L H V I A E A Q K E G G I A A I V D A E H A F D P T Y A R K L G V D I N A L L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L T I V E T L V R S G A V D V V V V D S V A A L V P Q A E L E G - - - - - E M G D S V M G L Q A 

G R V T E I Y G P E S S G K T T L A L H A I A E A Q K G G G I A A M V D A E H A F D Q T Y A K K L G L D I G S L L V S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S G A V D I V V V D S V A A L V P Q A E L E G - - - - - E M G D S V M G L Q A 

G R V T E I Y G P E S S G K T T L A L H A I A E A Q K N G G I A A L V D A E H A F D P T Y A R K L G V D I N A L L V S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S G A V D I I V I D S V A A L V P Q A E L E G - - - - - E M G D S V V G L Q A 

G R V T E I Y G P E S S G K T T L A L H A I A E A Q K E G G I A A L V D A E H A F D P T Y A R K L G V D I N S L L V S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S G A V D I V V V D S V A A L V P Q A E L E G - - - - - E M G D S V V G L Q A 

G R I V E I Y G P E S S G K T T L S M H C I A E A Q K A G G I A A F I D A E H A F D K T Y A E K L G I D T T N L L I S Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E H L I R S G A I D I I V I D S V A A L V P K A E I E G - - - - - E M G D S K M G L Q A 

G R I I E I Y G P E S S G K T T L A I H A I A E A Q K A G G L A A I I D A E H A F D R T Y A E K L G V N V D N L W I A Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E Q L I R S S A V D I I V I D S V A A L T P K A E I E G - - - - - E M G D N K V G L H A 

G R I I E I Y G P E S S G K T T L A I H A I A E A Q K A G G I A A F I D A E H A F D R F Y A A K L G V D V D N L W I S Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E Q L I R S S A I D I I V I D S V A A L T P K A E I E G - - - - - D M G D N K V G L Q A 

G R I I E I Y G P E S S G K T T L A I H A I A E A Q K A G G I A A F I D A E H A F D R F Y A A K L G V D V D N L F I S Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E Q L I R S S A I D I I V V D S V A A L T P K A E I E G - - - - - D M G D N K V G L Q A 

G R I I E I Y G N E S S G K T T L A L H S L A E V Q K L N G I V A F V D A E H A L D V E Y A R K L G V D V D K L I V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V D S L V R S N I V D L I V V D S V A A L V P K A E I E G - - - - - A M G D S H M G L Q A 

G R I V E I F G Q E S S G K T T L A L H A I A E A Q K M G G V A A F I D A E H A L D P V Y A K N L G V D L K S L L I S Q P D H - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V D E L V R S G V V D L I V V D S V A A L V P R A E I E G - - - - - A M G D M Q V G L Q A 

G R I V E I Y G P E S S G K T T I A L H A I A S A Q K N G G I A A F V D A E H A L D P V Y A K N L G V D V E N L L I A Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V D E L V R S N A V D L V V I D S V A A L V P R A E I E G - - - - - S M G D V Q V G L Q A 

G R I I E I Y G P E S S G K T T I A L H A I A S V Q K L G G I A A F V D A E H A L D L N Y A K N L G I D L S E L L V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L D I V D E L V R S N A V D L I V V D S V A A L V P R A E I E G - - - - - A M G D A Q I G L Q A 

G R V I E I Y G P E S S G K T T L A L H A I A E I Q K T G G V A A F V D A E H A L D P A Y A K V L G V N V D D L I I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - M A M E I V D Q L V R S A A I D V I V I D S V A A L V P R A E I E G - - - - - E M G D A H V G L Q A 

G R I I E V F G P E S S G K T S I A L H A I A E V Q K Q G G T A A F V D A E H A L D P T Y A A A L G V D I Q N L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - A A L E I V D Q L V R S A A V D I V V V D S V A A L V P R A E I E G - - - - - D M G D A H V G L Q A 

G R I I E V F G P E S S G K T S V A L H A I A E V Q K R G G T A A F V D A E H A L D P A Y A A A L G V D I Q N L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - A A L E I V D Q L V R S A A V D I V V V D S V A A L V P R A E I E G - - - - - D M G D A H V G L Q A 

G R I V E I Y G P E S S G K T T L A L H A V A A T Q Q A G G V A A F V D A E H A L D P V Y S K A L G V D I D N L L V A Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - S A L E I V D Q L V R S T A V D I I V V D S V A A L V P R A E I E G - - - - - E M G D T S V G S Q A 

G R I V E I Y G P E S S G K T T V A L H A I A E V Q K A G G V A A F I D A E H A L D P T Y S A A L G V D I E N L L V A Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - S A L E I A D Q L V R S A A V D L I V I D S V A A L V P R A E I E G - - - - - E M G D V Q V G L Q A 

G R I I E V Y G P E S S G K T T L T L H A I A E V Q K Q G G I A A F V D A E H A L D P V Y A T S V G V D I D N L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - M A L E I V D Q L V R S A A V D I V V I D S V A A L V P R A E I E G - - - - - E M G D A Q V G L Q A 

G R V I E I Y G P E S S G K T T V A L H A I A E V Q K E G G I A A F V D A E H A L D P T Y A S A L G V D I Q N L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - S A L E I V D Q L V R S A A V D I V V I D S V A A L V P R A E I E G - - - - - D M G D A H V G L Q A 

G R V I E I Y G P E S S G K T T V A L H A L A E V Q R N G G I A A F V D A E H A L D P T Y A A A L G V D I D N L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - S A L E I V D Q L V R S A A V D I V V I D S V A A L V P R A E I E G - - - - - D M G D A H V G L Q A 

G G V T L I G G E P G I G K S T L L L Q V A D K V A S R G G T V L Y V A G E E S L E Q I R L R A D R L G V A A D L Q M T R D T R A E H I A A L L E E H K P A L C I V D S I Q T V T V E G E G A P G G V A Q V R D G T A M L T R A A K E T G T A T V L V G H V T K D G T V A G P K V M E H I V D T T V F L E T 

































































Pld. phaeoclathratiforme RecA 
























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R L M S Q A L R K L T A V A N R T N T C L I F I N Q I R E K I G - - - - - - - V T W G S P E T T T G G R A L K F Y A S V R I D I R R T A S I K D G - E E - - - V I G S R T K A K I A K N K L A P P F K E V E F D I I Y G K G I C R E G D L L D L G V A H K L I E K S G T W F S L K S - - - - G E R L G Q G R 

R L M S Q A L R K L T G S V N K S R T C L I F I N Q I R E K I G - - - - - - - V M F G N P E T T T G G R A L K F Y S T V R L D I R R I G A I K D G - D A - - - V V G N R T K V K V V K N K V A S P F R E A E F D I I Y G E G V S K E G D L L D L G V A Q N L V E K S G S W F S Y K - - - - - G D R I G Q G R 

R L M S Q A L R K L T G T V S K S G T C L I F I N Q I R E K I G - - - - - - - V M F G N P E T T T G G R A L K F Y S S V R V D I R R I A A I K D G - D T - - - V I G S R T K V K V V K N K T A A P F R E A E F D I V Y G E G I S K E G D M L D L A V E A N I V E K S G S W F S Y K - - - - - T E R I G Q G R 

R L M S Q A L R K L T A S I G Q T R T A V I F I N Q L R E K V G - - - - - - - V M F G N P E V T P G G R A L K F Y S S V R I D L R R I E T I K Q G - T V - - - A I G T R V R A K V V K N K V A P P F R T A E F D I M F D S G I S R E G N L I D L G V T S E V I R K A G A F F S Y G - - - - - D I R L G Q G R 

R L M S Q A L R K L S G A V S K S R T A L I F I N Q L R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R K V E S I K Q G - Q D - - - V T G A R A R V K V V K N K V A P P F R Q A E F D I M Y N E G I S R E G N I V D V G V T M E I L R K S G A W F Y L G - - - - - D D R L G Q G R 

R L M S Q A L R K L S G A I S K S R A V V I F I N Q L R M K I G - - - - - - - V M F G S P E T T T G G Q A L K F Y A S V R L D I R R I E T L K Q G - Q E - - - A I G S R V R V K V I K N K V A P P F R Q A E F D I L A N E G I S R E G N I I D I G T E L G I I R K S G A W F Y L G - - - - - E D R L G Q G R 

R L M S Q A L R K L S G A I S K S R T V V I F I N Q L R M K I G - - - - - - - V M L G T P E T T T G G Q A L K F Y T S V R L D I R R V E T L K Q G - Q E - - - A I G S R V R V K V I K N K V A P P Y R Q A E F D I L A N E G I S R E G N I I D I G T E L G I I R K S G A W F Y L G - - - - - E D R L G Q G R 

R L M S Q A L R K L S G A I S K S R A V V I F L N Q L R L K I G - - - - - - - V M F G S P E T T T G G Q A L K F Y A S V R M D I R R I E T L K N G - Q E - - - T I G S R T R V K V V K N K V A P P F R Q A E F D I M H N E G I S R A G N I L D V G V E L D I I R K S G A W F Y L G - - - - - D D R L G Q G R 

R L M S Q A L R K L S G A I S K S R V V V I F L N Q L R L K I G - - - - - - - V M F G S P E T T T G G Q A L K F Y A S V R M D I R R I E T L K N G - Q E - - - T I G S R T R V K V V K N K V A P P F R Q A E F D I M H N E G I S R A G N I L D V G V E L D I I R K S G A W F Y L G - - - - - E D R L G Q G R 

R L M S Q A L R K L A G V T S K S K S I V V F I N Q L R E K V G - - - - - - - V M F G N P E T T P G G R A L K F Y A T I R L D V R K V D N I K Q G - N E - - - I V G S R T R V K V V K N K I A P P F K Q A E F D I M Y G E G I S R E G S I L D L G T A L D I I E K S G S W Y S Y K - - - - - D I K L G Q E E 

R L M S Q A L R K L A G V T S K S K T I V V F I N Q L R E K V G - - - - - - - V L F G N P E T T P G G R A L K F Y A T I R L D V R K V D A I K Q G - N E - - - I I G N R T R V K V V K N K I A P P F K Q A E F D I M Y G E G I S R E G S I L D I G T S I D I I E K S G A W Y S Y G - - - - - D I R L G Q G R 

R L M S Q A L R K L T G S I N K S K C A T I F I N Q L R E K V G - - - - - - - I M F G N P E T T P G G R A L K F Y S S V R L D V R K I E T I K Q G - D E - - - F L G S R T R V K V V K N K V A P P F K Q A E F D I M Y G T G I S F E G N V L D V G V D N E I I Q K S G S W F S Y N - - - - - D T R L G Q G R 

R L M S Q A L R K L A G S I N K S K C V A I F I N Q L R E K V G - - - - - - - I M F G N P E T T P G G R A L K F Y S S V R L D V R R I D S I K Q G - D Q - - - I L G N R T R V K I N K N K V A P P F K M A E F D I M Y N E G I S K V G N I L D V G V R E E L V D K S G S W F S Y K - - - - - D T R L G Q G R 

R L M S Q A L R K L T G S I N K S K C I A I F I N Q L R E K V G - - - - - - - I M F G N P E T T P G G R A L K F Y A S V R M D I R K I D T I K Q G - E E - - - M I G N R V R V K V V K N K V A P P F K Q A E F D I M Y N E G I S R E G N I V D V G V K E N I I Q K S G A W F S Y G - - - - - D I R L G Q G R 

R L M S Q A L R K L T G A I S K S R T T V I F I N Q I R E K V G - - - - - - - V V Y G S P E T T T G G R A L K F Y A S I R L E V R K Q E N I K Q G - T D - - - I I G S R T R V K V V K N K V A P P F K Q A D F D I M Y G T G I S K E G S I L D M A T E L N I V E K S G A W Y S F G - - - - - E E R L G Q G R 

R L M S Q A L R K L T G A I S K S K T V A I F I N Q M R E K V G - - - - - - - V M F G N P E T T P G G R A L K F Y A S I R M D V R K I D V I K Q G - N D - - - I I G S R T R V K I V K N K V A P P F K Q A D F D I M Y G E G I S K E G S L L D V A V E L K I V Q K V G S W Y S Y N - - - - - D E R L G Q G R 

R L M S Q A L R K L T G V V S K S K T C C L F I N Q I R E K V G - - - - - - - V M F G N P E T T P G G R A L K F Y S S V R L E V R K V D T L K Q G - S D - - - M I G S R T R V K V V K N K V A P P F K V A D F D I M Y G E G I S R E G S L V D L A V D M G I V E K S G A W Y S Y K - - - - - G D R L G Q G R 

R L M S Q A L R K L T G A I S K S N T C V I F I N Q I R E K V G - - - - - - - I M F G N P E T T T G G R A L K F Y A S V R L E V R R A D T L K Q G - Q D - - - M I G N R T K V K V V K N K V A P P F K Q A E F D I M Y G E G I S R E G S M V D V G A E L D I I N K S G A W Y S Y K - - - - - G E R L G Q G R 

R M M S Q A M R K L S A S I N K T K T I A I F I N Q L R E K V G V - - - - - - - M F G N P E T T P G G R A L K F Y A S V R L D V R G T T Q I K G T G D Q K D S S I G K E T K I K V V K N K V A P P F K V A E V E I M Y G E G I S R T G E L V K I A S D L D I I Q K A G A W F S Y N - - - - - G E K I G Q G S 

R L M S Q A L R K L S G A I S K S N T T A I F I N Q I R E K V G V - - - - - - - M F G N P E T T P G G R A L K F Y S S V R L E V R R A E Q L K Q G Q E - - - - I V G N R T K I K V V K N K V A P P F R V A E V D I M Y G Q G I S K E G E L I D L G V E N D I V D K S G A W Y S Y N - - - - - G E R M G Q G K 

R L M S Q A L R K L S G V I N K S K T I A I F I N Q I R E K V G V - - - - - - - M F G N P E I T P G G R A L K F Y S T V R L E V R R A E Q L K Q G T D - - - - V M G N K T K I K V V K N K V A P P F R I A E V D I M Y G E G I S R E G E L V D M A A E V D V I N K S G S W Y S Y K - - - - - E E R I G Q G R 

R L M S Q A L R K L S G A I N K S K T I A I F I N Q I R E K V G V - - - - - - - M F G N P E T T P G G R A L K F Y S S V R L E V R R A E Q L K Q G N D - - - - V M G N K T K I K V V K N K V A P P F R T A E V D I M Y G E G I S K E G E I I D L G T E L D I 

R L M S Q A L R K L S G A I N K S K T I A I F I N Q I R E K V G V - - - - - - - M F G N P E T T P G G R A L K F Y S T V R L E V R R A E Q L K Q G N D - - - - I V G N K T K V K V V K N K V A P P F R V A E V D I M Y G E G I S R E G E I L D M A S E L D I V Q K S G A W Y S Y N - - - - - E E R L G Q G R 

R L M S Q A L R K L S G A I N K S K T I A I F I N Q I R E K V G V - - - - - - - M F G N P E T T P G G R A L K F Y S T V R L E V R R A E Q L K Q G N D - - - - I V G N K T K V K V V K N K V A P P F R V A E V D I M Y G E G I S R E G E I L D M A S E L D I V Q K S G A W Y S Y N - - - - - E E R L G Q G R 

R L M S Q A L R K L K G A V H R S N T A L I F I N Q I R E K I G - - - - - - - V M F G N P E T T P G G R A L K F F S D M R L E V R R L G - D V K E G G E K K - - G - Y R V K V R V V K N K L A P P F Q E A E F D V I Y G E G I C R I C D I I D T A A N L G V I T K S G S W Y S Y G - - - - - E K R L G Q G R 

R L M S Q A L R K L K G A V H K S N T A L I F I N Q I R E K I G - - - - - - - V M F G N P E T T P G G R A L K F F S D M R L E V R R L G - D V K E G G E K K - - G - Y R V K V R V V K N K L A P P F Q E A E F D I I Y G E G I C K L C D L I E V A T N L G I F T K S G S W Y S Y G - - - - - D K R L G Q G K 

R L M S Q A L R K L T A I L S K T G T A A I F I N Q V R E K I G - - - - - - - V M Y G N P E T T T G G R A L K F Y A S V R L D V R K I G Q P T K V G N D A V - - A - N T V K I K T V K N K V A A P F K E V E L A L V Y G K G F D Q L S D L V G L A A D M D I I K K A G S F Y S Y G - - - - - D E R I G Q G K 

R L M S Q A L R K L T A V L A K S N T A A I F I N Q V R E K V G - - - - - - - V T Y G N P E T T P G G R A L K F Y A S V R L D V R K S G Q P I K V G N E A V - - A - V K V R V K V V K N K L A P P F R E A E L E I Y F G R G L D P V A D L V N V A V A A G V I E K A G S W F S Y G - - - - - E L R L G Q G K 

R L M S Q A L R K L T A V L A K S N T A A I F I N Q V R E K V G - - - - - - - V T Y G N P E T T P G G R A L K F Y A S V R L D V R K S G Q P I K V G N E A V - - G - V K V R V K V V K N K L A P P F R E A E L E I Y F G R G L D P V A D L V N V A V A A G V I E K A G S W F S Y G - - - - - E L R L G Q G K 

R L M S Q A L R K M T G A L Y N S G T T A I F I N Q L R E K I G - - - - - - - V M F G S P E T T T G G K A L K F Y A S V R C D I R R I Q T L K D G - Q D - - - A I G N R T R L K V V K N K V S P P F K I A E F D I M Y G E G I S R E S S V I D L A V D N G I V K K S G S W F T Y E - - - - - G E Q L G Q G K 

R L M S Q A L R K I A G A L S Q S K T T A I F I N Q L R E K V G - - - - - - - V F F G S P E T T S G G K A L K F Y A S V R I D V R R I E T L K E G - Q D - - - A V G N R T R A K I V K N K I A P P F K Q A E F D I I Y G Q G I S R E G S L I D M G V D N G I I R K S G S W F T Y D - - - - - G D Q L G Q G K 

R L M S Q A L R K I T G R L S Q T K T T A I F I N Q L R E K I G - - - - - - - V F F G S P E T T T G G K A L K F Y A S V R I D V R R I Q T L K E G - A D - - - S V G N R T K A K I V K N K M A P P F K I A E F D I I Y G Q G I S R E G G I I D M G V E H G I I K K S G S W F T Y D - - - - - G D Q L G Q G M 

R L M S Q A L R K I T S A L N Q S K T T A I F I N Q L R E K I G - - - - - - - V M F G S P E T T T G G R A L K F Y A S V R L D I R R I E T L K D G - T D - - - A V G N R T R V K V V K N K V A P P F K Q A E F D I L Y G Q G I S R E G G L I D M G V E N G F V R K A G A W Y T Y E - - - - - G D Q L G Q G K 

R L M S Q A L R K M T S A L N N S G T T A I F I N Q L R E K I G - - - - - - - V M F G S P E T T T G G K A L K F Y A S V R L D V R R I E T L K D G - S D - - - A V G N R T R V K V V K N K V S P P F K Q A E F D I L Y G Q G I S K E G S L I D M G V E Q G F I R K S G S W Y T Y E - - - - - G D Q L G Q G K 

R L M S Q S L R K L T G S I N R S K C M V I F I N Q L R M K I G - - - - - - - V M Y G N P E T T T G G N A L K F Y A S V R L D I R R T G Q I K D R - D E - - - V V G N S T R V K V V K N K V A P P F K Q V E F D I M Y G E G I S K I G E I L D L G V K A G I V E K S G S W F S Y D - - - - - S I R I G Q G R 

R L M S Q A L R K L T A S I S R S N C M V I F I N Q I R M K I G - - - - - - - V M Y G S P E T T T G G N A L K F Y A S V R L D I R R I G A I K E R - D E - - - V V G N Q T R V K V V K N K L A P P F K Q V E F D I M Y G E G I S K A G E L I D L G V K A G V V D K S G S W F S H D - - - - - S Q R I G Q G R 

R L M S Q A L R K L T A S I N K S N T M V I F I N Q I R M K I G - - - - - - - V M Y G S P E T T T G G N A L K F Y A S V R L D I R R I G Q I K E R - D E - - - V V G N Q T R V K V V K N K L A P P F K Q V E F D I M Y G E G V S K M G E I L D L G V K A G I V E K S G A W F S H D - - - - - S Q R L G Q G R 

R L M S Q A L R K L T A S I N K S H T M V I F I N Q I R M K I G - - - - - - - V M Y G S P E T T T G G N A L K F Y A S V R L D I R R I G A I K E R - D E - - - V V G N T T R V K V V K N K L A P P F K Q V E F D I M Y G E G V S K M G E I L D L G V K A G I V E K S G A W F S Y D - - - - - S Q R L G Q G R 

R L M S Q A M R K L T G S I S R S N C M V I F I N Q I R M K I G - - - - - - - V M F G S P E T T T G G N A L K F Y S S V R L D I R R I G A L K D R - D E - - - V V G N H T R V K V V K N K V A A P F K Q V E F D I M Y G E G I S K M G E L L D L G V A A G V V D K S G S W F S Y G - - - - - D E R I G Q G R 

R L M S Q A M R K L T A S I G R S N C M V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R R T G A I K D R - D N - - - V I G N T T K V K V V K N K V A P P F R E V E F D I M Y G E G I S K T G E L V D L G V K A G V V E K S G S W Y S Y G - - - - - D E R I G Q G R 

R L M S Q A M R K L T A S I G R S N C M V I F I N Q I R M K I G - - - - - - - V M F G S P E T T S G G N A L K F Y A S V R L D I R R T G A I K D R - D E - - - V I G N Q T R V K V V K N K V A P P F R E V E F D I L Y G E G I S K V G E L V D L G V K A G V V A K S G A W Y S Y G - - - - - D E R I G Q G R 

R M M S Q A M R K L A G N L K Q S N T L L I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R R T G A I K E G - D E - - - V V G N E T R V K V V K N K V A A P F K Q A E F Q I L Y G Q G I N R T G E L V D L G V A H K L I E K A G A W Y S Y K - - - - - G D K I G Q G R 

R M M S Q A M R K L A G N L K Q A N T L L I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R R T G A I K D G - E E - - - V V G S E T R V K V V K N K V A A P F K Q A E F Q I L Y G E G I N I H G E L V D L G V K H K L I E K A G A W Y S Y N - - - - - G D K I G Q G K 

R M M S Q A M R K L A G N L K Q S N T L L I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R R I G A V K E G - E N - - - V V G S E T R V K V V K N K I A A P F K Q A E F Q I L Y G E G I N F Y G E L V D L G V K E K L I E K A G A W Y S Y K - - - - - G E K I G Q G K 

R M M S Q A M R K L A G N L K Q S N T L L I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R R I G A V K E G - E N - - - V V G S E T R V K V V K N K I A A P F K Q A E F Q I L Y G E G I N F Y G E L V D L G V K E K L I E K A G A W Y S Y K - - - - - G E K I G Q G K 

R L M S Q A L R K L T G S I Q R S N T L V V F I N Q I R M K I G - - - - - - - V M F G S P E T T T G G N A L K F Y S S V R M D I R R L G A I K K G - D E - - - V L G N E T R V K V V K N K M A P P F K Q A E F E I L Y G E G I S R E G E I I D M G V A Q G F V D K S G A W Y S Y N - - - - - G D R I G Q G K 

R L M S Q A L R K L T A N I K K T N T L V I F I N Q I R M K I G - - - - - - - V M F G S P E T T T G G N A L K F Y A S V R L D I R R I G A I K K G - D E - - - V I G S E T K V K V V K N K V A P P F R E A I F D I L Y G E G I S R Q G E I V E M G V A H K L V D K S G A W Y A Y K - - - - - G E K I G Q G K 

R L M S Q A L R K L T A T I K R T N C M V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y S S V R L D I R R I G A I K K G - D E - - - V V G N E T R V K V V K N K V A P P F K Q A E F D I M Y G S G I S R E G E I I D L G V Q A N V V D K S G A W Y S Y S - - - - - G N R I G Q G K 

R L M S Q A L R K L T A T I K R T N C M V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y S S V R L D I R R I G A I K K G - D E - - - V V G N E T R V K V V K N K V A P P F K Q A E F D I M Y G S G I S R E G E I I D L G V Q A N V V D K S G A W Y S Y S - - - - - G N R I G Q G K 

R L M S Q A L R K L T G T I S K S Q T C V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S Q R L D I R R I G A I K N G - E N - - - V V G S R T R V K V V K N K V A P P F K E V E F D I M Y G T G I S R E G D L I D L A S N E N I V E K S G S W F A F K - - - - - G E R I G Q G R 

R L M S Q A L R K L T G T I S K S S T I V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A T Q R L D I R R I G A I K D G - D Q - - - V I G N R T R V K V V K N K V A P P F K E V E F D I M Y G H G I S R E G D L L D L G S N E N I V E K S G T W F S F G - - - - - G E R I G Q G R 

R L M S Q A L R K L T G T I S K S S T I V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S Q R L D I R R I G A I K D G - D S - - - V I G N R T R V K V V K N K V A P P F K E V E F D I M Y G H G I S R E G D V L D L A S N E G I V E K S G T W F S F G - - - - - G E R I G Q G R 

R L M S Q A L R K L T G I I S K S N C C V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R K I A T L K Q G - D Q - - - V I G S R T R V K V V K N K V A P P F K E A E F D I L Y G E G I S R T G D V L D L A V D R N I I D K S G A W F S Y N - - - - - K E R I G Q G R 

R L M S Q A L R K L T G I I S K S N C C V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R K I A S L K Q G - Q D - - - V I G S R T K V K V V K N K V A P P F K E V E F D I Y Y G E G I S R E G D I L D L A V E K G I V D K S G A W F S Y G - - - - - G D R I G Q G R 

R L M S Q A L R K L T G I I S K S N C C V I F I N Q I R M K I G - - - - - - - V M F G S P E T T T G G N A L K F Y A S V R L D I R K I A T L K Q G - D A - - - V I G S R T K V K V V K N K V A P P F R E V E F D I Y Y G E G I S R L G D L L D L A V D R K I I D K S G A W F S Y G - - - - - S D R I G Q G R 

R L M S Q A L R K L T G I V H K M N T T V I F I N Q I R M K I G - - - - - - A M G Y G T P E T T T G G N A L K F Y A S V R L D V R K V A T L K Q N - E E - - - P I G N R V K V K V V K N K V A P P F R Q A E F D V M F G E G L S R E G E L I D Y G V K L D I V D K S G A W F S Y K - - - - - D K K L G Q G R 

R L M S K A L R K L T G I L H K M N C T V I F I N Q I R M K I G - - - - - - M M G Y G S P E T T T G G N A L K F Y A S V R I D V R R I A S L K Q G - E S - - - Q I G N R V K A K V I K N K V A P P F R Q A E F D I M F G E G I S K E G E L V D Y G V K L D I I D K S G A W F S Y G - - - - - E T K L G Q G R 

R L M S Q A L R K V T G V L H K M N T T V I F I N Q I R M K I

Figure 10S 

100 

100 

63 

62 

99 

76 

100 

53 

100 

93 

Chl. vibrioforme 

Pld. luteolum 

Chl. phaeobacteroides BS1 

Ptc. aestuarii 

Chl. chlorochromatii 

Pld. phaeoclathratiforme 

Chl. limicola 

Chl. phaeobacteroides 

Chl. tepidum 

Cab. thermophilum 

Cfx. aurantiacus 

Cfx. aggregans 

Rhb. sphaeroides CrtF 

Rps. palustris CrtF 

Rvx. gelatinosus CrtF 

A. thaliana COMT

Cab. thermophilum BchU 

Cfx. aurantiacus BchU 

Cfx. aggregans BchU 

Chl. vibrioforme BchU 

Pld. luteolum BchU 

Chl. phaeobacteroides BS1 BchU 

Ptc. aestuarii BchU 

Chl. chlorochromatii BchU 

Pld. phaeoclathratiforme BchU 

Chl. limicola BchU 

Chl. phaeobacteroides BchU 

Chl. tepidum BchU 

Rps. sphaeroides CrtF 



A. thaliana ComT 

1 42 

1 44 

1 44 

1 51 

1 28 

1 28 

1 28 

1 29 

1 28 

1 28 

1 28 

1 28 

1 62 

1 58 

1 70 

1 51 

M S N P V S T A A E A L S T V R E E V L Y E Q Y L R - - - - A C D M T Y R G T V D M F V L K 

M T T V P S V D A T K T N G T D L T Q H D L F A A T N R - - - - A Y D M V F K G T V D F F V I K 

M T T A P S I D A T T T A G T A L E Q Q E L F A A T N R - - - - A Y D M V F K G T V D F F V I K 

M L P E P A A C E R L F L S I N C S Q Y M T M M N D I Q L L Q Q N E R - - - - A N E L I F K G L V E F G C F K 

M N E I A L L K Q N H R - - - - A N E L I F K G L V E F G C F K 

M N D N E L L K R N H R - - - - A N E I L F K G L V E F G C F K 

M N D N E L L K Q N H R - - - - A N E I V F K G L V E F G C F K 

M M N T N E L L N Y N H R - - - - A N E L V F K G L V E F G C I K 

M D A N E L L K Q N H R - - - - A N E L I F K G L V E F G C F K 

M N D L E L L K Q N H R - - - - A N E L I F K G L V E F G C F K 

M N D I E L L Q Q N H R - - - - A N E L C F K G L V E F G C F K 

M S N N D L L N Y Y H R - - - - A N E L V F K G L I E F S C M K 

M S A L R P P A R G G L G L S R L G V R L A G S R R F Q L I A E R V P F L R R I G R R E G E E L F D I V A G F V H S Q V L Y 

M V S L S L R D R L L G W R D S I L S N P R F Q R F A A V F P L M R P V A R R R A V A M F D L V A G F V Y S Q I L L 

M D R A E T R P E A G P R R S P T L A E R F R D W K H R T I A R P G F Q K W A A A F P L T R P V A R K H A R E L F D L L A G F V Y S Q I L L 

M I S L Q T S G G S S E E E D M L L A I Q L G G L N F V P Y I V K T A R - - - - - E L D L F E I M A K A R P L G 

















43 108 

45 108 

45 108 

52 115 

29 92 

29 92 

29 92 

30 93 

29 92 

29 92 

29 92 

29 92 

63 132 

59 128 

71 140 

52 120 

A A Y E L G L F R H L A D Q P Q S V E A L A A A T G S R L N R L V K F T D M L V E M E L L A K D A E G R L T L T P F A R Q F F L P P - - - - 

A A K D L G L F D L L A S A P R T L A D L A T L T E T V P P R L E K F L I T M E Q I G L V T R Q G D - A W Q L T P F A D Q F F A D - - - - - 

A A K D L G L F D L L A A E P R T L A D L A T L T E T V P P R L E K F L I T L E Q V G L V A R D A D - R W K L T P F A E Q F F A D - - - - - 

A A L E L D L F T H M A G S P K E A E T L A D D C Q A I P S R F V M L L E A L R Q I G I T L E S D G - K W S L T P L A R A M F V P - - - - - 

A A L E L D L F A L L A D G A K D T A T L A R E A G A V P P R L G M L L E A L R Q I G V T T E N A G - Q W D L T P M A R T M F V P - - - - - 

A A L E L D L F T E L A E E A K D A E T L S S A L E A V P Q R L E M L L E A L R Q I G I V R I E N G - K W A L T D F A R S M F V P - - - - - 

A A L E L D L F K H L A G E A K D V E S L A T S I E A V P Q R L A M L L E A L R Q I G I T R Q E D G - K W S L T P F A S N M F V P - - - - - 

A S L E L D L F T H L A G E A K D T E T I A A N V G A I P Q R L V I L L E T L A Q I G V L A K N D G - K W S L T P F A A T M F L P - - - - - 

A A L E L D L F T L L A D E A K N V E T L A A S A G A I P Q R L I M L L E A L R Q I G V T A E T G G - K W S L T P F A Q K M F L P - - - - - 

A A L E L D L F S Q L A D E A K D T G T L A A A T G S V P P R L G M L L E A L R Q M G V T N E D A G - K W S L T P S A K A M F V P - - - - - 

A A V E L D L F S L L A D E P K D T E D L A S S V G A V P M R L G M L L E A L R Q I G I T E E R D G - K W A L T S F S K A M F V P - - - - - 

A A I E L D L F S H M A E G P K D L A T L A A D T G S V P P R L E M L L E T L R Q M R V I N L E D G - K W S L T E F A D Y M F S P - - - - - 

A L V E L R V L H L V A E G P Q T V Q A L A A A T G L A P E R M Q F L L Q G G A A L K L L K P R R Q R Q F H L G G P G A A F L A V P G V Q A 

A C V Q L R L F D I V A E R P A T A E D L A A Q L S L P V D S L R V L L D G A V A L R L L Q M R S G H R Y G L G Q L G A E L R G N P G V L A 

A C V R L G V F K M L A D G P L P L A T V A E R C G L S L E A A R R L V A A A D S L E L L D L Q S G D L V T L G R L G A P L V A N E A I T A 

S Y L S P V D L A S M A - A P K N P H A P M M I D R L L R F L V A Y S V C T C K L V K D E E G R E S R A Y G L G K V G K K L I K D E D G F S 

















109 176 

109 176 

109 176 

116 183 

93 160 

93 160 

93 160 

94 161 

93 160 

93 160 

93 160 

93 160 

133 195 

129 193 

141 209 

121 188 

N P N N E N L T M E P F V R Y A I T I M E R Y H L H L A K V I R G E M D F T A L T P W P P - - R T R E D S A F Y E E I H R S N N H F V R N L 

P E R H R N M T M V P F V D Y I A D L I E N Y Y L R L A D V V R G K V D F T S I V P H P P - - R T R E D S L F Y E T L H R S N I Q L F T E L 

P D R H R N M T M V P F V D Y I S D L I E N Y Y L R L A D V V R G K V D F T S I V P H P P - - R T R E D S L F Y E T L H R S N I Q L F V E L 

Q A D Q P N M Y M V P V G K A M A H L S E N Y Y L K I A D A V R G K H E F R A E V P Y P P - - I S R E D N L Y F E E I H R S N A Y F A I K L 

A A D Q P N M F M V P V A K A M A H L A D S F Y L K M A D A V K G N L N F K G E V P Y P P - - V T R E D N W Y F E E I H R S N A H F A I K L 

S D E H P N L Y M T P V A K A M A Y T A E N F Y M H M A E A V R G K L D Y K G E T S Y P P - - K T R E E N L Y F E D I H R R N A H F S I K L 

N D E H P N L Y M S P V A E A M A Y T A E N F Y M H M A Q A V K G K L D Y K G E T S Y P P - - K T K E E N L Y F E E I H R R N A H F S I K L 

N N E L P N L Y M M P V T K A M A H L S E N F Y L K I A D A V R G N H I F K A E V P Y P P - - M T R E D N W Y F E E I H R S N A H F S I L L 

N H E N Q N L Y M I P V A K A M A N L S E N F Y L Q I A D A V R G N M N F K G E V A Y P P - - V T R E D N L Y F E E I H R S N A Y F A I M L 

D A E H P N L Y M I P V A K A M A H L S D N F Y L K L A D A V K G Q L N F K G E V P Y P P - - V T R E D N W Y F E E I H R S N A H F A I K L 

N S E H P N M Y M T P V A R A M V H L S D N F Y M D L A S A V K G T M N F K G E V P Y P P - - V T R E D N W Y F E E I H R S N A H F A I K L 

T P K E P N L H Q T P V A K A M A F L A D D F Y M G L S Q A V R G Q K N F K G Q V P Y P P - - V T R E D N L Y F E E I H R S N A K F A I Q L 

M W G H H H V L Y R D L A D P V A F L K G E T E P E L A R F W P Y V F G A G G A T D P - - - - - - - E V T A K Y S R L M T E S Q G L V A E D 

L V A H H A M L Y R D L A D P V A L L R A P R G G G E L A A Y W A Y V R G E R P G E - - - - - L P A D A I A S Y T A L M A A S Q P M I A R E 

M V E H H A T L Y G D L T D P V A L L R G - Q G R P A M A G Y W P Y A A A A E A G P G Q P A T L A S E K V A E Y S K L M T A S Q P L V A R E 

I A P Y V L A G C T K A K G G V W S Y L T E A I Q E G G A S A W E R A N E A L I F E Y M K - - K N E N L K K I F N E S M T N H T S I V M K K 

















177 245 

177 244 

177 244 

184 251 

161 228 

161 228 

161 228 

162 229 

161 228 

161 228 

161 228 

161 228 

196 263 

194 261 

210 277 

189 250 

L V E E A D - L E G V T H L L D V G G G N G D I A I A L A K R F P S I E R I T L I N L P S A L D I V R D N V A A H G L S D R I V P V A L D M 

L I K R A R - L A D V Q T L I D V G G G I G D I A A A L C Q A F P Q L K - V T L I N L P S A L D L V R E N V A A K G L A D R I T P V A I D M 

L V K R A R - L A D V Q T L V D V G G G I G D I A A A L C Q A F P N L K - V T L I N L P S A L D L V R E N V A A K G L S D R I T P V A I D M 

L L E A A E - L S G T K N L V D V G G G I G D I S A A L L K K Y P E L N - S T I L N L P G A I G L V D E N A A A Q G V G G R L K G A A V D I 

L L E E A R - L E G T K T L V D V G G G I G D I S A A L L K K H P Q L D - S T I L N L P G A I G L V D E N A V A Q G V G G R L R G A A V D I 

L L E E A D - L Q N N K K M V D V G G G I G D I S A A L C N K F S Q L D - T T I L N L P G A I E L V N E N A A E K G L A D R L R G A A V D I 

L L E E A R - L D D T K K M I D V G G G I G D I A A A L C K H Y P N L D - T T I L N L P G A I E L V D E N A A E K G L G D R L R G A A V D I 

L L E E A N - L S N V K T L V D V G G G I G D I S A A L L Q K Y P Q M D - S T I L N L P G A V E L V N E N A T E K G L A D R L R G S V V D I 

L L E E A D - L E T A K K L V D V G G G I G D I S A A L L K K F P Q L H - S T I L N L P G A V E L V N E N A V E K G V D N R L K G A A V D I 

L L E E A D - L S A A K T M V D V G G G I G D I S A A L L Q K F T N L Q - S T I L N L P G A I E L V N E N A I E K G V G D R L K G V A V D I 

L L E E A D - L S A A K T L V D V G G G I G D I S A A L L K K Y T G L Q - S T I L N L P G A V P L V N D N A A E K G V A D R L R G A V V D I 

L L E E A K - L D G V K K M I D V G G G I G D I S A A M L K H F P E L D - S T I L N L P G A I D L V N E N A A E K G V A D R M R G I A V D I 

A L R L V D - L M G V R R L M D V G G G T G A F L A A V G R A Y P L M E - L M L F D L P V V A E A A P Q R L T E A G L A G R F T V H G G S F 

V L H V Y S - F R D H R C L L D V G G G D G S F L S A V A A Q A P Q L R - C M L F D L P A V A E K A A A R F E A N G L K G R A T A V G G S F 

V I A A Y R - F G R H K V L M D V G G G E G A F V R C V A A E V P G V E - L R V F D L P A V A E R A N E N F Q R W G L A G R A Q A F G G D F 

I L E N Y I G F E G V S D F V D V G G S L G S N L A Q I L S K Y P H I K - G I N F D L P H I V K E A P Q I H G V E H I G G - - - - - - - D M 

















246 314 

245 312 

245 312 

252 319 

229 296 

229 296 

229 296 

230 297 

229 296 

229 296 

229 296 

229 296 

264 333 

262 331 

278 347 

251 318 

Y R D P Y P A - C D A V L F A R I L Y P F N A Q L C A M L L G K A H A A L P P G G R V V V A D M L L R D E R G L A N Y D Y L A H F L T S I G 

Y R E P Y P P - G D A V L F S R I L Y P M N A Q F C T M L L R K A Y D A L P S G G R I L I L D M V I S D P N - H P N Y D Y L T H Y L C A I G 

Y R D P Y P P - G D A V L F S R I L Y P M N A Q F C S M L L K K A Y D A L P S G G R V L I L D M V I S D P Q - H P N Y D Y L T H Y L C A I G 

Y K E A Y P S - A D A V M F C R I L Y S A N E Q L T G M M C R K A Y D A L P A G G R I L I L D M I V D N P E - E P N F D Y L S H Y I L G A G 

Y K D A Y P A - A D A V M F C R I L Y S A N E Q L T E M M C R K A Y D A V P A G G K V L I L D M I V D D R E - N P N F D Y L S H Y I L G A G 

Y R D E Y P A - A D A V M F C R I L Y T A N E Q L T E M M C T K A F N A L E A G G K L L V L D M I V D E Q D - N P N Y D Y L S H Y I M G I G 

Y R D E Y P A - T D A V M F C R I L Y S A N E Q L T E M M C T K A F N A L S P G G K V M V L D M I V D D P S - N P N Y D Y L S H Y I M G I G 

Y K E E Y P K - A D A V M F C R I L Y S A N E Q I T S M M C K K A L D A L Q E G G K V L I L D M I I D E P E - D P N F D Y L S H Y I L G A G 

Y K D A Y P E - A D A I M F C R I L Y S A N E Q L T T M L C K K A Y D A L T P G G K V L I L D M I I D D P E - N P N F D Y L S H Y I L G A G 

Y K D V Y P K - A D A V M F C R I L Y S A N E Q L S E M M C R K A F E A L P S G G K V L I L D M I I D D P E - S P N F D Y L S H Y I L G A G 

Y K E A Y P Q - A D A V M F C R I L Y S A N E Q L T D M M C R K A F E A L S A G G K L I I L D M V I D D P E - T P N F D Y L S H Y I L G A G 

Y K E S Y P E - A D A V L F C R I L Y S A N E Q L S T I M C K K A F D A M R S G G R L L I L D M V I D D P E - N P N F D Y L S H Y I L G A G 

R D D P L P L G A D A I S L V R V L F D H S D E T V K L L L H R V R E A L P A G G R V I V A E A M S G G A R P H R E T D T Y M A F Y T A A M 

T T D P L P E G A D I V S L V R V I H D H D D E V V E A L L R A V H K A L P D N G K L L I A E P I A G L P G T A A I S D A Y F A F Y L Y A M 

F A D E L P R G A D V I S L V R V A F D H P D E R V L T L F K N V R R A L P D N G T L V L A E A M A E V P G V E P I G D A Y F G F Y L L A M 

F - D E I P R - G E V I L M K W I L H D W N D E K C V E I L K N C K K A L P E T G R I I V I E M I V P R E V S E T D L A T K N S L S A D L T 

















315 356 

313 354 

313 354 

320 362 

297 339 

297 339 

297 339 

298 338 

297 339 

297 339 

297 339 

297 338 

334 379 

332 373 

348 390 

319 363 

V D P A I M T F K R Q - - D E Y F D V F K Q L G F I D A R K V E R H G H V L Y Q A R K A 

M G F S V L E F K D H - - A V Y P D L L R Q V G F T D V T L D E G Y E H V L Y Q A V K P 

M S F S V L E F K D H - - A I Y P D L L R Q I G F T D V T F D E G Y D H V L Y Q A V K P 

L P F S V L G F K S Q - - E S Y R G I L E E I G F S D I S I T R R Y N H L L C Q A V K K G 

M P F S V L G F K S Q - - E A Y R Q I L E S I G F T D V R M V R K Y D H L L C E A V K P A 

M P F S V L G F K E Q - - S N Y K N I L E K I G F S D V R M V R R Y D H L Y V E A V K P S 

M P F S V L G Y K D Q - - D N Y K H I L E K I G F T D V R M V R R Y D H L F V E A V K P A 

M P F S V L G F K Q Q - - E R Y K E L L E A I G F R D V R I V R K Y G H L L C E A V K 

M P F S V L G F K Q Q - - S A Y K E I L E S L G F R D I R T V R K Y G H L L C E A V K A L 

M P F S V L G F K P Q - - N A Y K E I L E K I G F T G V R I V R R Y D H L L C E A V K P A 

M P F S V L G F K P Q - - S A Y K E I L E A I G F T D V R M V R K Y D Q L L C E A V K P A 

M P F S V L G F K E Q - - A R Y K E I L E S L G Y K D V T M V R K Y D H L L V Q A V K P 

R T G R V R S A A E I - - A E L L T G Q G F S E I K I F P G L R P Y V A S A V T A V R P S D A P 

G S G K A R S F P R - - - - - L Q A M L E N A G F S Q V T L H P V P M P L V A S V I T A S K R 

G R G R P R S A A R - - - - - L T Q M L H E A G F A S V R P L S T H M P L Q A G V L V A R C T A 

M M S L T S G G K E R T K K E F E D L A K E A G F K L P K I I Y G A Y S Y W I I E L Y P N 

Figure 11S

Figure 12S 

AcsF Alignments 

Cab. thermophilum AcsF 

Cfx. aggregans AcsF 

Cfx. aurantiacus AcsF 

Rfx. sp. RS1 

Rfx. castenholzii AcsF 

Rps. palustris AcsF 

Rb. sphaeroides AcsF 

Rb. sphaeroides 2.4.1 AcsF 

Synechocystis sp. 6803 AcsF 

G. violaceus AcsF 

Nostoc sp. 7120 AcsF 

Por. yezoensis AcsF 

A. thaliana AcsF 

10 20 30 40 50 60 70 80 90 100 110 

M I A T E S V I R E T K A A A A D K S L I S P R F Y V T D Y K K I N A L R F D H - - - - - M A E A F K A M H K R F A D D P N K H F 

M F W I N P L M M E M P G E F S R T T R H A L K E A I L S P R F Y T T D F K A I D K L E I D R N G - - - L R D E F D W I R D E F A Y D Y N R T H 

M F W I N P L M M E M P G E F S R T T R H A L K E A I L S P R F Y T T D F K A I D R L E I D R N G - - - L R D E F D W I R D E F A Y D Y N R T H 

M M N I P G E Y T P T T R H A L R E A I L N P R F Y T T D F R A I D R L N V A H - - - - - M R D E F D W I R N E F E V D Y N R K H 

M I N M P G E Y S P T T R H A L R E A I L N P R F Y T T D F R A I D R L N V A H - - - - - M R D E F D W I R N E F E F D Y N K K H 

M I P M E G G A Q G A L R T N S R P A I K G S V D S L N I A K E D T I L T P R F Y T T D Y A A M D K L D V S L - - - - - V R S E W N A M M N E M R A D Y N K A H 

M N A S P G D L S P A L D P E A V A D T T A M A T E T H I L N P R F Y T T D F D E L D R V D V T P - - - - - I R A Q W D E L I A E M K A D P N K G H 

M N A P A G G L S P A L T P E A V A D T T A M A T E T H I L N P R F Y T T D F D E L D R V D V T P - - - - - V R K D W D A L I A E M K A D P N K A H 

M V N T L E K P G F D E I R P G V K T P A K E T I L T P R F Y T T D F D E M A K M D I S P N - - - - - E D E L R A I L E E F R V D Y N R H H 

M V D A L R E A N T A S Q S E R R E T L L T P R F Y T T D F E A L A K L D I S A M - - - - - E A D F E A L L A E F Q A D Y N R E H 

M V D S L K K P G F E E I R P G V K V P A K E T L L T P R F Y T T D F D E M A R M D I S V N - - - - - E D E L L A I L E E F R T D Y N R H H 

M Q T T I N N G Q T S S K E T L L T P R F Y T T D F E E M A N M D I S G N - - - - - Q E D F L A I L E E F R A D Y N S E H 

M A A E M A L V K P I S K F S S P K L S N P S K F L S G R R F S T V I R M S A S S S P P P P T T A T S K S K K G T K K E I Q E S L L T P R F Y T T D F E E M E Q L F N T E I N K N L N E A E F E A L L Q E F K T D Y N Q T H 

M A A E M A L V K P I S K F S S P K L S N P S K F L S G R R M . E T T . A . . E . I L . P R F Y T T D F . . D . L D . . N G K N L R . E F . A I . E F . . D Y N . H 




Rfx. sp. RS1 










120 130 140 150 160 170 180 190 200 210 220 

F R R D D - - - D P D F D Q D F S H L P P S F K D F L I R G C I G E F S G A L L Y K E I A D R I E D - - P I I S D T Y R L M A R D E G R H S G F I R L A M R D I N T D L D L Q M L Q K E K E F V S M H P K I I L Y T T Y L S 

F R R T D E F L Q N F D D M P E - - - R D A F I D F L E R S C T A E F S G C L L Y A E M V K H L H - - D P T L R A I F K Y M S R D E G R H A G F L N R T M A D L N V A L D L T V L Q K R K K Y T Y F Q P K F I F Y S V Y L S 

F R R T D E F L Q D F D D M P E - - - R D S F I D F L E R S C T A E F S G C L L Y A E M V K H L H - - D P T L R A I F K Y M S R D E G R H A G F L N R T M A D L N V A L D L T V L Q K R K K Y T Y F Q P K F I F Y S V Y L S 

F V R N D E F L A N F D D M P A - - - R D L F I E F L E R S C T A E F S G C L L Y A E M V K H L H - - D P T L K A I F R C M S R D E G R H A G F L N K T M A D L G V E M N L Q V L H T R K K Y T Y F Q P K F I F Y S V Y L S 

F V R N E E F L A N F D D M P A - - - R D L F I E F L E R S C T A E F S G C L L Y A E M V K H L H - - D P T L R A I F R C M S R D E G R H A G F L N K T M A D L G V E M N L Q V L H T R K K Y T Y F Q P K F I F Y S V Y L S 

F K K T D E F L E S D L D K L P P E L L I E F K D F L V S S L T A E F S G C V L Y S E I K K R I K - - N P E I R E L F G L L S R D E A R H A G F I N E I L K D H G I G V D L S F L T K V K K Y T Y F R P K F I F Y A T Y L S 

F K K T D A W D R I D W E A M D P A L R V E F I D F L V S S C T A E F S G C V L Y K E M K R R G S - - N P D I C E L F S Y M A R D E A R H A G F I N D A L R E A G V A V N L G F L T K A K K Y T Y F R P K F I Y Y A T Y L S 

F K K T E A W D R I D W D G M D P A L R V E F I D F L V S S C T A E F S G C V L Y K E M K R R G S - - N P D I R E L F N Y M A R D E A R H A G F I N D A L R E A G V A V N L G F L T K A K K Y T Y F R P K F I Y Y A T Y L S 

F V R N E S F N K S W D H I D G E - K R Q L F V E F L E R S C T A E F S G F L L Y K E L G R R L K N K N P L L A E C F N L M S R D E A R H A G F L N K A M S D F N L S L D L G F L T K S R K Y T F F K P K F I F Y A T Y L S 

F Q R D A G F V E Q A H Q I D P Q - W R A L M L E F L E R S C T S E F S G F L L Y K E I S R R L K A A N P L L A E C F K A M S R D E A R H A G F L N K A M G D Y D L G L D L S N L A K Q K K Y T F F K P K F I F Y A T Y L S 

F V R D A E F E K S W D H I D G D - T R R L F V E F L E R S C T A E F S G F L L Y K E L G R R L K D K S P V L A E C F N L M S R D E A R H A G F L N K A M S D F N L S L D L G F L T K S R N Y T F F K P K F I F Y A T Y L S 

F I R D E E F N Q S W S N L E H K - T K S L F I E F L E R S C T A E F S G F L L Y K E L S R K L K D R N P V I A E C F L L M S R D E A R H A G F L N K A I G D F N L S L D L G F L T K S R K Y T F F S P K F I F Y A T Y L S 

F V R N K E F K E A A D K L Q G P - L R Q I F V E F L E R S C T A E F S G F L L Y K E L G R R L K K T N P V V A E I F S L M S R D E A R H A G F L N K G L S D F N L A L D L G F L T K A R K Y T F F K P K F I F Y A T Y L S 

F R . E F . D D . L R . F I E F L E R S C T A E F S G C L L Y K E . . R R L . N P L E . F . M S R D E A R H A G F L N K A M D . N . . L D L F L T K K K Y T Y F . P K F I F Y A T Y L S 




Rfx. sp. RS1 










230 240 250 260 270 280 290 300 310 320 330 

E V I G Y F R Y I N I Y S H L E A H P E Y R Y H P I F K Y F G G W C Q D E L K H G D F I G L Q L E A Q K H L Y L S G G I N R L L I R F F S L A V Y V T M W L R D L K A Q E F Y A S I G L N W R E Y D L K V I R E T N A Y A Q 

E K I G Y S R Y I N I Y R H L E Q N P Q Y C I H P I F K W F E Q W C N D E Y R H G E F F A L L M R S Q P E M L E G - - A N R R W I R F F L L A V Y A T M Y L N D A R R A N F Y A A L G L N W R D Y D Q K V I R L C N D I A T 

E K I G Y S R Y I T I Y R H L E R N P Q Y C I H P I F K W F E Q W C N D E Y R H G E F F A L L M R S Q P E M L E G - - V N R R W I R F F L L A V Y A T M Y L N D A R R S N F Y A A L G L D W R D Y D Q T V I R L C N D I A T 

E K I G Y A R Y I T I Y R H L Q K Y P Q G M I H P I F K W F E K W C N D E Y R H G E F F S L L M R S Q P D L L R G - - G N L R W I R F F L L A V Y A T M Y L N D A R R A G F Y Q A L G L D W R D Y D Q R V I R L T N H I A T 

E K I G Y A R Y I T I Y R H L Q H H P Q G M I H P I F K W F E K W C N D E Y R H G E F F S L L M R S Q P D L L R G - - G N L R W I R F F L L A V Y A T M Y L N D A R R A G F Y E A L G L N W R D Y D Q R V I R L T N H I A T 

E K I G Y A R Y I T I Y R Q M E R H P E R R F H P I F K W F E R W C N D E F R H G E A F A L L M R A D P S L L R G - - V N K L W I R F F L L A V F A T M Y V R D H M R P A F Y D A L G V D A T D Y D M Q V F R V T T E I S K 

E K I G Y A R Y I T I Y R H L E A N P E H R F H P I F K W F K E W C N D E F R H G E A F A L L M K T D P K L T D S T - V N R L W I R F F L T A V Y S T M W V R D H A R P E F H K A L G V D I D W Y D Q E V F R K T S E I A R 

E K I G Y A R Y I T I Y R H L E A N P E H R F H P I F K W F K E W C N D E F R H G E A F A L L M K T D P K L T D T T - V N R L W I R F F L T A V Y S T M W V R D H A R P E F H K A L G V D I D W Y D Q E V Y R K T S E I A R 

E K I G Y W R Y I T I Y R H L E K N P N D C I Y P I F E F F E N W C Q D E N R H G D F F D A I M R A Q P H T L N D W - K A K L W C R F F L L S V F A T M Y L N D T Q R A D F Y A C L G L E A R S Y D K E V I E K T N E T A G 

E K I G Y W R Y I A I F R H L E R H P E N R I H P L F N Y F K N W C Q D E N R H G D F F G L I L K S Q P Q L L K G P - I N R L W V R F F L L S F C L T M Y L N D R K P H N L Y S A L G L D A S H Y A M E V L R Q T N L D S G 

E K I G Y W R Y I T I Y R H L E A H P E D R I Y P I F R F F E N W C Q D E N R H G D F F D A I M K S Q P Q M L N D W - K A K L W S R F F L L S V F A T M Y L N D I Q R K D F Y A T I G L D A R D Y D I Y V I K K T N E T A G 

E K I G Y W R Y I T I Y R H L E Q H P E H R I Y P I F R F F E N W C Q D E N R H G D F F A A L L K S Q P H F L N D W - K A K M W C R F F L L S V F A T M Y L N D F Q R I D F Y N A I G L D S R Q Y D M Q V I R K T N E S A A 

E K I G Y W R Y I T I Y R H L K E N P E F Q C Y P I F K Y F E N W C Q D E N R H G D F F S A L M K A Q P Q F L N D W - Q A K L W S R F F C L S V Y V T M Y L N D C Q R T N F Y E G I G L N T K E F D M H V I I E T N R T T A 

E K I G Y R Y I T I Y R H L E P E R I H P I F K W F E . W C N D E . R H G E F F . L L M . S Q P L L G . N . L W I R F F L L A V Y A T M Y L N D R F Y A L G L D R . Y D V I R T N . I A 




Rfx. sp. RS1 










340 350 360 370 380 390 400 410 420 430 440 

R V F G L R L D V D N P K F I R I L D H M A A C Y A K M L K R E A D G A S K L A L M P L Q V A M A V D Y L R L L A M K V R T D A P P P I P E Y P V A P V I K G F A T K Y K G Q Y K G Q P A M A * 

Q V Y P E T M N L D D P R F F G Y M D K L V E Y D R K L R E L E G R K D P V A L A Q S T R L K A A I A L R L L A I Y R L P T R K T T E A I R W K G L P G F P N Y P G P N R P Q R V A A A D 

Q V Y P E T M N L D D P R F F A Y M D K L V E Y D R A L R A L E S R K D P I A M A H S T R L K A A I A L R L L A I Y R L P T R K T T E A I R W K G L P G F P N Y P G P N R P Q R V V A A D 

Q V F P V T L P V D D P R F F R H L D A C V T Y D A Q I R A L E G R S D P I A T A Q R A R L G A A I A A R L L A T Y R L P V V P T T D A N R W Q G L E G F P N Y P G P G W K Q D A T L N Q R V I S A 

Q V F P V T L P V D D P R F F R H L D A C V R Y D A Q I R A L E G R N D P I A Q V Q R A R L G A G I A A R L L A T Y R L P P A P T T D A N R W K G L E G F P N Y P G P G W K Q D A S L N E R V I S A 

Q V F P V M I N L D D P R F L A G L E R L R K V S E K I A E - S R S Q G F V G K L K R P L Y A A S A A I A F G R L F L L P A K R N E L P R V I G L R P A W 

Q I F P V E L D I D H P R W K P A L N R M N A A F L R M D R G N R R G G L A G R L D K V L G G A Q A L A A F T A L Y T I P V R T N A L P A N V R L E P S Y 

Q I F P V E L D I D H P R W K P A L R R M N E A F L R I D R G T R R G G I A G R L E K A L G G A Q A L A A F V S L Y T I P V R T H T L P E N V R L E P S Y 

R V F P I I L D V N N P E F Y N R L E T C V S N N E Q L R A I D A S G A P G V I K A L R K L P I F A S N G W Q F I K L Y L M K P I A V D Q L A G A V R 

K V F P L I L D L D N P R F Q R H L D R C A D L N L Q L E A L S G K S P - - - - - - L A R L P V I A A L A G T I A Q L F L L K P V A P P A P E M V R 

R V F P I I L D V D S P E F Y E R L D I C V E N S E K L S A I A S S N T P K F L Q F F Q K L P V L A S T G W Q L L R L Y L M K P I D A V S A Q G A A R 

R V F P V A L D V D N P K F F K Y L D T C A C D N R A L I D I D N N N S P L F I K S I V K I P L Y F S L F A N L L K I Y L I K P I D S K T V W N T V R 

R I F P A V L D V E N P E F K R K L D R M V V S Y E K L L A I G E T D D A S F I K T L K R I P L V T S L A S E I L A A Y L M P P V E S G S V D F A E F E P N L V Y 

Q V F P . L D V D P R F . L D . . V . L A . . . P . . . . . L A . . . . . . . . . . P . P . . . . P N Y P G P Q R V I S A

Figure 13S 

absorbance at 667 nm 

absorbance 

14 

12 

10 

8 

6 

4 

2 

0 

-2 

Absorbance spectrum 

of peak 3b 

436 

400 600 800 

Bchl c F 

wavelength (nm) 

667 

Bchl c GG 

1 2 3 4 1 2 3 4 

15 20 25 30 35 40 45 50 

elution time (min) 

1a 

1b 

2b 

2a 

3a 

3b 

4b 

Chl a

Table 1S. Oligonucleotide primers used in this study. 

Gene Description Forward (5’ to 3’) Reverse (5’ to 3’) 

Cab. thermophilum 16S rRNA Ribosomal RNA used 

as phylogenetic 

marker 

Anoxybacillus sp. 16S rRNA Ribosomal RNA used 


marker 

Bacterial 16S rRNA Ribosomal RNA used 


marker 

Cab. thermophilum recA DNA repair protein 

used as phylogenetic 

marker 

Cab. thermophilum pscA Type 1 reaction center 

protein 

Cab. thermophilum fmoA BChl a-binding 

protein 

Cab. thermophilum csmA BChl a-binding 

protein located in the 

baseplate of 

chlorosomes 

Cab. thermophilum bchU C-20 

methyltransferase for 

BChl c biosynthesis 

Cab. thermophilum acsF Oxidative cyclase for 

BChl a,c biosynthesis 

under oxic conditions 

GAT CCT GGC TCA 

GAA TC (38) 

CGA AAG TCT GAC 

GGA GCA AC 

AGA GTT TGA TCA 

TGG CTC AG (46) 

GCA TGG AAG GCG 

CTG TAG CC 

GCT TTT CGT CCT 

ATG CCA ACG 

GGC AAG ATT ACG 

GTC GGA AAG 

GTC GTG GAC TCC 

ATC GTC 

TTC TCT ACG AGC 

AGT ATT TGC GTG 

CAG GAT TTC TCC 

CAT CTC CCA C 

GGC TTA CAC AGG 

ATA CC 

TCA TCG TTT ACG 

GCG TGG AC 

GGT TAC CTT GTT 

ACG ACT T (46) 

CAG ACA GCC TGC 

TTC CTA CC 

AGG GGT CAA ACC 

AAC CGT AAG G 

CCC TCA ATA GGA 

ACA CTA AAC CCC 

TCC TTC GGC TGT 

GAA ACG 

CGG TGG ATT TCC 

TCA TAA AAC G 

CCA ATA AAG TCG 

CCG TGT TTG AT

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