37 7th Veterinary Report - Department of Primary Industries ...
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<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
Prepared on 7 March 2010 by :<br />
Dr. Roger SM CHONG BVSc MACVSc<br />
Principal <strong>Veterinary</strong> Officer (Aquatic Animal Biosecurity)<br />
Biosecurity Queensland<br />
<strong>Department</strong> <strong>of</strong> Employment, Economic Development and Innovation<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
Page 1 <strong>of</strong> 27
Contents<br />
<strong>Veterinary</strong> Investigations Executive Summary ………………………………………………………………………………………..3<br />
Silver Perch Kill, Bass Deformity and Neurologic Syndromes …………………………………………………………………..6<br />
Mullet Deformities Syndrome …………………………………………………………………………………………………………………9<br />
Bass Fry Kill Syndrome ……………………………………………………………………………………………………………………………10<br />
Golden Perch Loss Syndrome ………………………………………………………………………………………………………………….12<br />
Australian Bass Growth Retardation Syndrome ……………………………………………………………………………………..14<br />
Silver Perch Larval Malformation,Discoordination and Acute Death Syndrome ……………………………………..16<br />
Supporting Documents ………………………………………………………………………………………………………………………….18<br />
Literature ………………………………………………………………………………………………………………………………………………20<br />
Acknowledgements ……………………………………………………………………………………………………………………………….26<br />
Photos cover page :<br />
Upper 1 st row ‐left – Silver perch deaths associated with spraying activity.<br />
Upper 1 st row‐right – Australian Bass twin body deformity from Noosa broodfish – histology.<br />
Upper 2 nd row left – Mullet embryo with three tails from Noosa broodfish.<br />
Upper 2 nd row right – Noosa mullet broodfish which produced deformed progeny.<br />
Lower 1 st row left – Fish pond and macadamia trees.<br />
Lower 1 st row right – Australian bass fingerlings with retarded growth.<br />
Lower 2 nd row left – Australian Bass fry from non‐Noosa broodfish, no deformities.<br />
Lower 2 nd row right – Golden perch gills with hypertrophic pathology, associated with nonylphenol exposure.<br />
(Photos with are Copyright 2010 Gwen Gilson.)<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
Page 2 <strong>of</strong> 27
<strong>Veterinary</strong> Investigations Executive Summary<br />
The Noosa Fish Health Investigation Task Force was a unique and timely opportunity to study and record the risks<br />
and effects <strong>of</strong> agrichemicals and pesticides on fish health. It was a complex investigation which utilised the<br />
strengths <strong>of</strong> veterinary science with its multidisciplines <strong>of</strong> epidemiology, pathology, parasitology and toxicology.<br />
As a result, a clearer understanding <strong>of</strong> the fish health syndromes was achieved in the past year. The interplay <strong>of</strong><br />
the risk factors involved with agrichemical use and the responses <strong>of</strong> a highly sensitive group <strong>of</strong> animals – fish, has<br />
been methodically described as six major syndromes. This provides a practical foundation from which to harness<br />
and apply effective solutions. It will ensure that the health <strong>of</strong> the horticultural, aquacultural and ecological assets<br />
<strong>of</strong> the Noosa river catchment are sustainably protected into the future from inadvertant agrichemical<br />
contamination.<br />
Silver Perch Kill, Bass Deformity and Neurologic Syndromes<br />
Silver Perch Kill<br />
The epidemiological evidence was consistent with the silver perch being at high risk <strong>of</strong> acute exposure to very<br />
low sublethal but toxic doses <strong>of</strong> beta‐cyfluthrin pesticide. The pesticide, in combination with gill hyperplasia<br />
pathology caused by gill fluke parasites and moderate level background heavy metals was a significant additional<br />
stress to the fish. When high environmental temperatures occurred concurrent with pesticide spray days, the<br />
combined interplay <strong>of</strong> risk factors produced a major fish kill at the hatchery pond <strong>of</strong> silver perch. The fish were<br />
normal right up till the week <strong>of</strong> beta‐cyfluthrin spraying by the macadamia plantation.<br />
Australian Bass Deformity<br />
Pathology confirmed the two headed deformity in fish embryos reported and this appeared to indicate<br />
interferance with the control mechanisms <strong>of</strong> the embryonic cells. The implication would be reproductive failure<br />
<strong>of</strong> the bass population in the Noosa river as fewer young fish are produced or survive. Normal bass fry were<br />
produced by Sunland Hatchery when non‐Noosa broodstocks are used; which strongly points to the Noosa river<br />
as being the source <strong>of</strong> potential chemical toxins with mutagenic and genotoxic properties.<br />
Golden Perch Neurological Derangement<br />
A pathological lesion <strong>of</strong> spinal inflexion and fracture was consisitent with reports <strong>of</strong> partially atropine responsive<br />
treatment <strong>of</strong> spinning problems with fish larvae at Sunland Hatchery. This supports the real risk <strong>of</strong> exposure to<br />
organophosphate type pesticides. Spray log data confirmed that methidathion (an organophosphate) was used in<br />
the period associated with observation <strong>of</strong> spinning or convulsive symptoms in the fry particularly after water<br />
from ouside the hatchery building was used in spawning tanks.<br />
Mullet Deformities Syndrome (MDS)<br />
MDS pathology is very similar to the two headed bass syndrome because three tails or axial multiplication<br />
occurred. The serious implication is that a common agent is causing reproductive damage to more than one<br />
species <strong>of</strong> fish in the Noosa river. MDS was clinically presented as resulting in the failure <strong>of</strong> normal, full‐term<br />
development <strong>of</strong> fertilised mullet eggs. Carbendazim and atrazine agrichemicals were detected in the catchment<br />
at very low levels which appear to be innocuous, but is it ? given the epidemiological evidence <strong>of</strong> a potential<br />
common link.<br />
Bass Fry Kill Syndrome<br />
Evidence <strong>of</strong> liver and red blood cell pathology in affected fish pointed to potential methoxifenozide<br />
contamination <strong>of</strong> algal feeds held in uncovered tanks outside from spray drift <strong>of</strong> macadamia operations leading<br />
to acute deaths <strong>of</strong> hatched Australian Bass. Fish that were not fed the algae on the days <strong>of</strong> agrichemical spraying<br />
did not experience similar mortalities.<br />
Golden Perch Loss Syndrome<br />
Parasitology detected an external parasite Costia sp. protozoan as the main cause <strong>of</strong> low survival from 10,000 fry<br />
stocked in a pond. Interestingly, pathology also detected toxic injury to gill, liver and red blood cells not related<br />
to Costia sp.. Carbendazim, nonylphenol, trichlorphon/dichlorvos, 4‐t‐octylphenol, bisphenol A and urea<br />
agrichemical residues were detected from water storage locations on the Gilson Rd. hatchery. This was clear<br />
evidence <strong>of</strong> contamination from macadamia spray drift for carbendazim, nonylphenol and<br />
trichlorphon/dichlorvos.<br />
Australian Bass Growth Retardation Syndrome (ABGRS)<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
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ABGRS resulted in a statistically significant (P
Develop effective tools for sensitive monitoring <strong>of</strong> river ecosystems from the potential risks <strong>of</strong><br />
agrichemical run‐<strong>of</strong>f.<br />
Where agrichemicals are found to have unacceptable toxicity risks to the ecosystem under practical<br />
conditions <strong>of</strong> use, withdrawal from market is an option.<br />
Until clear outcomes are in progress, the Sunland Hatchery will continue to manage the agrichemical<br />
contamination risks experienced whch resulted in the last five years <strong>of</strong> fish health and production failures<br />
through support from Biosecurity Queensland and the hatchery’s private veterinarian ‐ Dr. Matt Landos, using a<br />
Hatchery Spray Drift Protection Program. This program entails ‐<br />
a. Protection <strong>of</strong> all water sources within an enclosed structure.<br />
b. Protection <strong>of</strong> all fish and algae production units with screens or covers to minimise drift <strong>of</strong><br />
pesticide and other agricultural chemicals.<br />
c. Use <strong>of</strong> an alternate site for the sustainable production <strong>of</strong> grow out sized fish.<br />
d. Use <strong>of</strong> an alternate site for the production <strong>of</strong> food items for larval fish eg. algal and<br />
zooplankton.<br />
This program has successfully resulted in the production <strong>of</strong> healthy and fecund Australian Bass, silver perch and<br />
golden perch fingerlings for sale in late 2009 and early 2010. However, this mode <strong>of</strong> biosecurity adds<br />
considerable costs to the production <strong>of</strong> fish for restocking groups and aquaculturists because <strong>of</strong> increased labour<br />
inputs to carting clean water from the Ringtail ponds, transportation <strong>of</strong> normal fish to Ringtail, decontamination<br />
procedures for utensils and equipment <strong>of</strong> the hatchery at Gilson road and increased monitoring <strong>of</strong> macadamia<br />
plantation spray activity.<br />
Prepared on 7 March 2010 by :<br />
Dr. Roger SM CHONG BVSc MACVSc<br />
Principal <strong>Veterinary</strong> Officer (Aquatic Animal Biosecurity)<br />
Biosecurity Queensland<br />
<strong>Department</strong> <strong>of</strong> Employment, Economic Development and Innovation<br />
Pr<strong>of</strong>itable <strong>Primary</strong> <strong>Industries</strong> for Queensland<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
Page 5 <strong>of</strong> 27
Silver Perch Kill, Bass Deformity and Neurologic Syndromes<br />
The veterinary investigation commissioned by the Noosa Fish Health Investigation Task Force (NFHITF) into the<br />
Sunland Fish Hatchery silver perch fish kill, golden perch neurological abnormalities and Australian bass<br />
deformities has examined the available sources <strong>of</strong> information and has come to validated conclusions which<br />
specifically address the fish health problems.<br />
Silver Perch Fish Kill<br />
The field and laboratory evidence are consistent with the silver perch at high risk <strong>of</strong> being exposed to very low<br />
sublethal but toxic doses <strong>of</strong> beta‐cyfluthrin pesticide. The pesticide, in combination with gill hyperplasia<br />
pathology caused by gill flukes and heavy metals was a significant stress to the fish. When high environmental<br />
temperatures occurred concurrent with pesticide spray drift, the chain <strong>of</strong> events produced a major fish kill at the<br />
hatchery pond <strong>of</strong> silver perch.<br />
The current laboratory and veterinary epidemiological data is supported through spray drift modeling conducted<br />
by the Australian Pesticides and <strong>Veterinary</strong> Medicines Authority (APVMA) AgDRIFT model. AgDRIFT<br />
demonstrated that all fish ponds on the hatchery would receive pesticides including beta‐cyfluthrin even when<br />
applied under current pesticide label conditions. The potential averaged initial concentrations <strong>of</strong> pesticides<br />
achieved in the fish hatchery water storages by spray drift were :<br />
o Without buffer<br />
o With buffer<br />
Cardendazim 0.013 – 0.162 µg/L<br />
Methidathion 0.027 – 0.323 µg/L<br />
beta‐cyfluthrin 0.00068 – 0.0082 µg/L<br />
Cardendazim 0.007 – 0.079 µg/L<br />
Methidathion 0.013 – 0.158 µg/L<br />
beta‐cyfluthrin 0.0006 – 0.004 µg/L<br />
By extrapolating from literature sources, the toxicity end points for silver perch when based on the Bluegill<br />
sunfish demonstrate a number <strong>of</strong> toxic exposure scenarios :<br />
1. The 10% lethal concentration <strong>of</strong> beta‐cyfluthrin has the potential to cause temperature<br />
stress effects at an exposure <strong>of</strong> 0.087 – 0.15 µg/L.<br />
2. Swimming abnormality in fish can be due to toxic effects at 0.7 – 5% <strong>of</strong> the lethal<br />
concentration at an exposure <strong>of</strong> 0.0006 – 0.0075 µg/L beta‐cyfluthrin.<br />
3. An estimated safe level for exposure to beta‐cyfluthrin for silver perch is up to 0.00087<br />
µg/L.<br />
From these, an estimated cyfluthrin exposure level <strong>of</strong> the order <strong>of</strong> 0.00087 – 0.15 µg/L (ppb) cyfluthrin could<br />
contaminate the pond sufficient to result in a sublethal toxic effect on silver perch resulting in fish temperature<br />
tolerance and swimming behaviour changes. Given that the AgDRIFT model exposure level is estimated to be<br />
0.0006 ‐ 0.0082 µg/L (ppb), this fitted within the range for sublethal toxicity.<br />
On its own this range <strong>of</strong> beta‐cyfluthrin exposure would not be expected to result in temperature stress effects<br />
or mortalities. However fish with pre‐existing pathology such as gill hyperplasia when linked with successive heat<br />
waves would stress the fish beyond the point <strong>of</strong> survival. By addressing the significant and additional stress<br />
factors in an epidemiological framework <strong>of</strong> cause and effect, the veterinary risk assessment arrived at a more<br />
realistic estimation <strong>of</strong> the toxic impact for the pesticide. Thus the veterinary risk assessment indicated that the<br />
AgDRIFT beta‐cyfluthrin exposure is between 4.6 and 9.4 times the approximate cyfluthrin No Observable Effect<br />
Concentration (NOEC) for silver perch. This translates to a significant health risk to the silver perch fish. The<br />
concept <strong>of</strong> a causal web involving multiple stressors on the fish and ecosystem health is a well recognised<br />
phenomenon by ecotoxicologists Carson (1962) and Heath (1998) because sublethal toxic effects at extremely<br />
low concentrations can have significant consequences in the aquatic environment particularly where species are<br />
exposed to many different stressors occurring concurrently.<br />
The available information from the veterinary investigation show that the silver perch have been exposed to a<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
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toxic level <strong>of</strong> beta‐cyfluthrin which was undetectable with current laboratory test sensitivities, in the water<br />
samples or in fish tissue samples but yet significantly contributed to the eventual deaths <strong>of</strong> the fish .<br />
Australian Bass Deformity<br />
Histology has confirmed the lesions described by Sunland Hatchery in that fish formed twin bodies/heads. The<br />
early deformities appear to indicate interferance with the control mechanisms governing distribution <strong>of</strong> the cells<br />
in the embryo rather than causing the death <strong>of</strong> embryonic cells. This may involve abnormalities with the<br />
hormonal control mechanisms <strong>of</strong> the blastomere and if it is due to a chemical compound its source may need to<br />
be considered as originating in the yolk material (ie. broodstock source) or in the water source used for the<br />
spawning <strong>of</strong> these eggs. Given that broodstocks from a non‐Noosa River source produced normal fry using the<br />
same water as that used for the broodstocks producing defective fry, the Noosa broodstock may more likely be<br />
the source <strong>of</strong> the problem. If these deformity lesions exist in the Noosa River bass over several breeding seasons,<br />
the implication would be a significant decline in the recruitment rate <strong>of</strong> juvenile fish in the hatchery and an<br />
eventual loss <strong>of</strong> the population <strong>of</strong> bass in the river unless intervention through the stocking <strong>of</strong> viable fry occurs.<br />
There is a requirement to examine the role <strong>of</strong> pesticides as teratogens in aquatic species. For example, atrazine<br />
has been shown to cause embryonic teratogenic effects in catfish (Berge et.al. 1983). Non‐pesticide teratogens<br />
such as heavy metals also need to be considered in the context <strong>of</strong> the aquatic environment <strong>of</strong> teratogenic effects.<br />
In the context <strong>of</strong> the veterinary epidemiology the issue <strong>of</strong> discovery <strong>of</strong> deformed Australian Bass at Sunland<br />
Hatchery is not related to husbandry or hormonal issues because the hatchery succesfully spawned normal bass<br />
fry when broodstock from the wild other than from the Noosa River were used for breeding. This is a critical<br />
epidemiological pointer that should guide the investigation into potential issues with the Noosa River which may<br />
be impacting the recruitment and replenishment <strong>of</strong> fish stocks over a period <strong>of</strong> years. To this end, the spray drift<br />
modelling data on the hatchery and nut farm are not directly relevant to the risk assessment <strong>of</strong> embryonic<br />
deformities reported by the hatchery. The epidemiological evidence suggests that since beta‐cyfluthrin and<br />
methidathion are much more toxic than carbendazim and that the frequency <strong>of</strong> carbendazim application is<br />
product label restrained to a maximum <strong>of</strong> 2 applications, together with the spray record indication that<br />
carbendazim was only used in 2007 (and not prior in 2005 or 2006), the likelihood <strong>of</strong> carbendazim being a major<br />
cause <strong>of</strong> fish kills is low.<br />
Golden Perch Neurological Derangement<br />
A histological lesion <strong>of</strong> spinal inflexion and fracture is not in‐consistent with reports <strong>of</strong> partially atropine<br />
responsive treatment <strong>of</strong> neurological problems with fish and supports the real risk <strong>of</strong> exposure to<br />
organophosphate type pesticides. Spray log data confirmed that methidathion was used in the period associated<br />
with observation <strong>of</strong> spinning or convulsive symptoms in the fry particularly after water from ouside the hatchery<br />
building ie. from the blocked tanks that have been modelled by AgDRIFT to receive pesticides including levels <strong>of</strong><br />
methidathion in the range <strong>of</strong> 0.027 to 0.323 µg/L based on a reported 2008/09 application rate <strong>of</strong> 1030 L/ha.<br />
However the spray records showed that methidathion was not applied in that period. Rather methidahion was<br />
used in 2007 and the application rate from spray records indicated that up to 1396 L/ha was used at the time <strong>of</strong><br />
the neurological symptoms observed in the fish fry held in tanks inside the hatchery or from broodstocks which<br />
were in the ponds during the spray period. This would potentially result in a 35.5% increase <strong>of</strong> drift concentration<br />
<strong>of</strong> 0.0<strong>37</strong> – 0.438 µg/L. The LC50 95h for the most sensitive species <strong>of</strong> Bluegill sunfish is 0.9 – 5.1 µg/L (averaged<br />
2.2 µg/L). Given that the golden perch (and silver perch) fry did not suffer an acute fish kill but continued with<br />
neurological symptoms for several days and recovered in 72h with atropine therapy – the exposure pr<strong>of</strong>ile <strong>of</strong><br />
methidathion is up to 49% that <strong>of</strong> the acute mortality but still below. There is therefore a significant risk <strong>of</strong><br />
exposure leading to clinical signs consistent with acute toxicity without causing a mass fish kill.<br />
It is interesting to note from an epidemiological perspective that all 3 syndromes <strong>of</strong> fish at Sunland Hatchery can<br />
be explained by exposure to 3 classes <strong>of</strong> pesticides. This would be improbable if husbandry, water quality,<br />
nutritional, bacterial, viral, fungal or parasitic disease were a problem at the hatchery. For example, it would be<br />
highly improbably for a single pathogen to manifest the 3 different syndromes <strong>of</strong> acute fish kills, neurological<br />
symptoms and embryonic fish deformities as no such pathogen exists. Different pathogens would need to be<br />
present all together at a virulent level on the farm and this is not the case after the veterinary sampling and<br />
testing for viral, fungal, bacterial and parasitic agents. Further for husbandry to be an issue, it would have to be<br />
so far removed from industry standards as to be unsustainable. This is just not the case with Sunland Hatchery<br />
which has been for 26 years a reliable producer <strong>of</strong> quality fish for many stocking groups and including the<br />
Queensland Government. What the epidemiology consistently points to is an issue with pesticide use activity for<br />
the period 2005 onwards, this being the time <strong>of</strong> onset <strong>of</strong> a series <strong>of</strong> fish kills, losses and health problems.<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
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<strong>Report</strong> Recommendations<br />
1. The veterinary investigation recommends an adverse event report process for the APVMA to be<br />
initiated in relation to beta‐cyfluthrin. This signals the need to conduct structured research into<br />
pesticide exposure and toxicity to aquatic species such as commercial fish because existing label<br />
regulations do not appear to safe guard against spray drift in certain situations <strong>of</strong> pesticide use<br />
identified in this report. Options for this research are presented in this report.<br />
2. The veterinary investigation recommends on‐going structured surveillance into the Australian Bass<br />
deformity syndrome based on pathological confirmation that a significant early embryonic disorder is<br />
present with the potential to impact bass recruitment and replenishment in the Noosa River<br />
Catchment. Options for surveillance research are presented in this report.<br />
3. The veterinary investigation recommends the development <strong>of</strong> a risk mitigation plan for the Sunland<br />
Fish Hatchery which is able to ensure sustainable production <strong>of</strong> valuable native Australian fish species.<br />
This plan should contain in its core requirement specific strategies to protect fish culture from<br />
inadvertant spray drift with pesticide chemicals. Options for risk mitigation are presented in this report.<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
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Mullet Deformities Syndrome<br />
Mullet Deformities Syndrome (MDS)<br />
o Is a new and serious condition affecting the reproductive health <strong>of</strong> mullet fish in the Noosa River with a<br />
potential to alter the general mullet population recruitment rate. This is dependant on the prevalence<br />
<strong>of</strong> MDS in the mullet population.<br />
o Has significant similarites to the deformity syndrome described for Australian Bass from the Noosa<br />
River.<br />
o It would not be inconsistent to consider that the reason or risk factor for MDS to be the same as that<br />
for the deformities in Australian Bass fry <strong>of</strong> the Noosa River. The risk factor causes reproductive failure<br />
in both species.<br />
o Is caused by a non‐infectious agent(s) with potential genotoxic or mutagenic properties, being present<br />
in the ecosystem where mullet and bass co‐habitat. Exposure period(s) in the Noosa River may increase<br />
or decrease the risk <strong>of</strong> MDS in mullet.<br />
Case Definition – Mullet Deformities Syndrome (MDS)<br />
MDS is defined as a disorder <strong>of</strong> the reproductive health <strong>of</strong> Mugil cephalus currently detected in fish sourced from<br />
the Noosa River system. MDS is clinically presented as resulting in the failure <strong>of</strong> normal, full‐term development<br />
<strong>of</strong> fertilised mullet eggs from the stage <strong>of</strong> egg activation , through cell division, embryonic organogenesis and to<br />
hatch. The clinical signs appear to be more severe in mullet which commercial fishers consider to be Noosa River<br />
resident fish. Clinical signs and pathologies are variously presented as deformites <strong>of</strong> cell development (eccentric<br />
cells, halos, tophats) and deformities <strong>of</strong> organogenesis (axial duplication and axial triplication, dwarfs and snake<br />
forms) in the formed embryo. Clinical sign also appears to reflect an arrestment and/or an abortive effect on<br />
apparently fertilised eggs in the absence <strong>of</strong> visible deformities. The net result <strong>of</strong> MDS being a failure <strong>of</strong> effective<br />
reproduction from fish in normal spawning condition and in fish with no evidence <strong>of</strong> significant disease from<br />
viral, bacterial, fungal, parasitic or chlamydial pathogens. MDS appears to be independent <strong>of</strong> husbandry factors .<br />
The recommendations <strong>of</strong> this report are –<br />
A. Fish surveillance program to –<br />
o Monitor the impact <strong>of</strong> MDS to mullet and <strong>of</strong> two headed deformity to Australian Bass populations by<br />
comparing Noosa to non‐Noosa fish in fish breeding trials.<br />
o Surveillance <strong>of</strong> recruitment rates <strong>of</strong> mullet and bass in the Noosa river over a period <strong>of</strong> several years.<br />
B. Agrichemical exposure trials to ‐<br />
o Understand the genotoxic and mutagenic effects <strong>of</strong> agrichemicals detected in the Noosa catchment to<br />
fish reproductive performance.<br />
o Assess the toxicity levels <strong>of</strong> carbendazim and atrazine to reproductive performance <strong>of</strong> Australian Bass<br />
and mullet in controlled experimental conditions.<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
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Bass Fry Kill Syndrome<br />
The incident <strong>of</strong> acute mortalities <strong>of</strong> Australian Bass at Sunland Hatchery was investigated and risk assessed with<br />
respect to spray activities at the adjacent macadamia plantation. The results <strong>of</strong> the investigation form the 3 rd<br />
<strong>Veterinary</strong> <strong>Report</strong> <strong>of</strong> the Noosa Fish Health Investigation Task Force. On the balance <strong>of</strong> the evidence at the<br />
epidemiological, pathological and toxicological levels, the risk <strong>of</strong> adverse effect in the bass fry points towards<br />
chemical toxicity with methoxifenozide based on :<br />
a. The pathology <strong>of</strong> the affected bass fry supports that <strong>of</strong> a toxicological process affecting both erythrocyte and<br />
hepatocellular functions. These changes have been reported for methoxyfenozide in animal species.<br />
b. There is temporal correlation between spray activity employing methoxyfenozide and urea potential for<br />
exposure to the chemicals via the route <strong>of</strong> ingestion <strong>of</strong> algae that was located outside the hatchery.<br />
c. Biotoxins from algal and bacterial sources have been assessed to be unlikely on the basis <strong>of</strong> clinical<br />
epidemiology.<br />
d. There is evidence <strong>of</strong> exposure to both chemicals reported used in macadamia spraying activities due to<br />
spray drift onto water storage locations on the hatchery.<br />
e. Available toxicological data for fish species does not indicate that the toxic concentration <strong>of</strong><br />
methoxyfenozide or urea has been reached for an acute lethal effect given a very low estimated toxicity risk<br />
ratio.<br />
f. The areas <strong>of</strong> uncertainty are :<br />
the concentrations <strong>of</strong> methoxyfenozide in affected fish<br />
the sensitivity <strong>of</strong> bass fry to methoxyfenozide<br />
mechanism <strong>of</strong> toxicity <strong>of</strong> methoxyfenozide in fish<br />
Elucidation <strong>of</strong> these issues on the basis <strong>of</strong> new research is likely to clarify the actual risk <strong>of</strong><br />
methoxyfenozide for Australian Bass in such a localised setting.<br />
The recommendations <strong>of</strong> this report are :<br />
A. Structured monitoring <strong>of</strong> spray drift and fish health indices to evaluate actual risks <strong>of</strong> spray practices <strong>of</strong> the<br />
macadamia plantation on hatchery operations.<br />
This should involve the use <strong>of</strong> sentinel fish to measure the actual contamination level <strong>of</strong> chemicals that may be<br />
applied through spray rigs at the macadamia plantation. It is important that the spraying practices reflect what is<br />
performed in the past and well as what is currently the routine. The monitoring should include collection <strong>of</strong><br />
environmental samples such as water, sediment and vegetation (for example leaves blown into ponds from the<br />
macadamia farm) for residue analyses <strong>of</strong> the specific chemicals used. This will provide more accurate data over<br />
time than that which is currently available either by ad‐hoc sampling or by spray drift modelling. Correlation <strong>of</strong><br />
results with wind speed and direction data as well as measurements <strong>of</strong> actual spray rates from the spray rigs are<br />
necessary for a robust assessment.<br />
This is necessary to understand the responses <strong>of</strong> native fish species <strong>of</strong> both larval, juvenile and adult life stages to<br />
the varied exposure scenarios. This information should include residue testing and histopathology with gross<br />
pathology as well as observations <strong>of</strong> clinical or sublethal behavioural changes. Information from this type <strong>of</strong><br />
surveillance would be useful to define the toxicity endpoints for the key pesticides and agri‐chemicals used for<br />
local species <strong>of</strong> fish.<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
Page 10 <strong>of</strong> 27
B. As the hatchery is a commercial operation, precautionary spray drift protection must be in place while<br />
structured studies are conducted. These include :<br />
e. Protection <strong>of</strong> all water sources within an enclosed structure<br />
f. Protection <strong>of</strong> all fish and algae production units with screens or covers to minimise drift <strong>of</strong><br />
pesticide and other agricultural chemicals<br />
g. Use <strong>of</strong> an alternate site for the sustainable production <strong>of</strong> grow out sized fish<br />
h. Use <strong>of</strong> an althernate site for the production <strong>of</strong> food items for larval fish eg. algal and<br />
zooplankton<br />
Refinement <strong>of</strong> the spray drift protection procedures currently adopted is needed to ensure that the<br />
additional costs <strong>of</strong> such measures are sustainable and appropropriate to the actual or defined spray drift<br />
risks. Without this refinement, it would be difficult to come to an agreeable basis for mediation that is<br />
required for the fish hatchery and macadamia plantation to co‐exist productively.<br />
C. An adverse reaction report should be compiled on the use <strong>of</strong> methoxyfenozide to the Australian Pesticides<br />
and <strong>Veterinary</strong> Authority for the purposes <strong>of</strong> :<br />
i. Conduct <strong>of</strong> a review <strong>of</strong> the active compound and label conditions<br />
ii. Conduct <strong>of</strong> research on the toxicity <strong>of</strong> methoxyfenozide to Australian Bass fry<br />
An adverse reaction report is the trigger to signal the need to conduct targeted research studies for a<br />
registered product used under label conditions but which still results in unintended effects in non‐target<br />
species. Information from the veterinary reports 1‐3 may be used to assist in the development <strong>of</strong> an adverse<br />
reaction report management strategy which specifically addresses the issues <strong>of</strong> information gaps for end‐<br />
point toxicity limits for local conditions and local species <strong>of</strong> fish.<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
Page 11 <strong>of</strong> 27
Golden Perch Loss Syndrome<br />
The Golden Perch Loss Syndrome was a replication <strong>of</strong> historical reports <strong>of</strong> fish health problems at Sunland<br />
Hatchery. This was the first instance when an adverse event in fish production had been observed from the<br />
commencement to the end <strong>of</strong> the reported spray period. It thus provided a much more complete opportunity<br />
than previously possible to identify the primary cause <strong>of</strong> the fish health problem.<br />
1. The primary cause <strong>of</strong> the Golden Perch Fish Loss Syndrome is the parasitic activity <strong>of</strong> large numbers <strong>of</strong> a<br />
protozoan ectoparasite – Costia sp.(or Ichthyobodo sp.) mainly infesting the skin <strong>of</strong> the very small fry<br />
stocked into the Barra pond. Costia sp. can result in acute mortalities <strong>of</strong> more susceptible fry. However fry<br />
can be rendered more susceptible when other concurrent tissue pathology is present. The likely source <strong>of</strong><br />
the parasite involved the use <strong>of</strong> water collected from a dam which has a connection to Cooloothin Creek.<br />
2. The Golden Perch Fish Loss Syndrome has a significant toxic component resulting in pathological injury to<br />
the gills, liver and erythrocytes <strong>of</strong> the fish. This cannot be explained by the presence <strong>of</strong> parasitic Costia sp..<br />
These toxic responses can increase the susceptibility <strong>of</strong> the injured fish to pathogens including Costia sp. by<br />
increasing the stressor factors active on the fish. The pathological changes <strong>of</strong> gill hypertrophy, eosinophilic<br />
cytoplasmic change and vacuolation, liver changes <strong>of</strong> hepatocellular eosinophilic microgranule<br />
accummulation, vacuolation and mild degeneration as well as increased immature erythrocyte response to a<br />
probable haemolytic disorder are consistent with exposure to toxin because mechanisms <strong>of</strong> toxicity to<br />
produce these changes are within the scope <strong>of</strong> action <strong>of</strong> several chemical toxins detected. Research work is<br />
required to define the respective roles <strong>of</strong> these chemical toxins.<br />
3. Carbendazim, nonylphenol, 4‐t‐octylphenol, and bisphenol A pesticide and urea residues were detected in<br />
samples from water storage locations on the Gilson Rd. hatchery. Dichlorvos was detected in the hatchery<br />
shed using air sampling filter paper. This constitutes clear evidence <strong>of</strong> contamination with pesticides and<br />
agrichemical pesticides. Of these, carbendazim and nonlylphenol containing products has been reportedly<br />
used by macadamia operations in the adjacent farm. Information from the spray record would need to be<br />
examined to assess the likelihood <strong>of</strong> these agrichemicals originating from the macadamia farm in terms <strong>of</strong><br />
temporal relationships to spray activity in question and the onset‐duration <strong>of</strong> the fish loss syndrome.<br />
4. There is clear evidence <strong>of</strong> a range <strong>of</strong> toxic effects resulting in the fish where specific protection from<br />
agrichemical spray activity has been removed by placing the fish in the Barra pond. Since toxic changes<br />
which may be attributable to one or more <strong>of</strong> these pesticides were observed in the affected fish, it is not<br />
conclusive that the contamination scenario observed in this case represents a ‘No Observable Effect Level’<br />
<strong>of</strong> exposure despite meeting the published criteria for toxicity endpoint assessments. That is, though<br />
published toxicity data support the notion that the fish should be safe, the observed field and laboratory<br />
data indicated that the fish were not safe. Safe meaning the absence <strong>of</strong> toxicological injury or responses in<br />
the tissues <strong>of</strong> the fish. As none <strong>of</strong> published acute lethal or chronic toxic concentrations had been reached<br />
and no detectable pesticide residue was found in the Barra pond where the affected fish were held, it was<br />
highly unlikely that pesticides directly killed the fish in this syndrome. If the assumption that these residue<br />
levels represent the actual exposure scenario in the field, then there should not be toxic pathological<br />
responses in the fish. This assumption needs to be tested. Given that these residue results were an averaged<br />
concentration <strong>of</strong> the samples, there is a potential issue with localised ‘hotspot’ effect where fish may be<br />
exposed to a higher concentration <strong>of</strong> chemical at the point should the spray drift droplets land on the<br />
surface <strong>of</strong> the water. The issue <strong>of</strong> increased risk to toxicity from mixture effects <strong>of</strong> pesticide exposure is<br />
possible because the existing toxicity endpoint references values are only based on individual chemical<br />
toxicities. These factors could potentially lead to an underestimation <strong>of</strong> the true exposure risk and toxicity<br />
end points. Again these issues need to be further researched because the field and laboratory data suggests<br />
an anomaly with the standard approach to toxicity end point assessment.<br />
5. Of particular interest is the liver pathology which suggests a role for endocrine disruptor compounds (EDCs).<br />
4‐t‐octylphenol, nonylphenol and bisphenol A are compounds with known EDC activity and their detection<br />
as residues in conjunction with liver changes suggestive <strong>of</strong> vitellogenic production warrants additional<br />
investigation to confirm. Nevertheless the current evidence is consistent with this assessment.<br />
6. Golden perch which were <strong>of</strong> similar age, spawning batch and time <strong>of</strong> stocking produced no toxic changes in<br />
the tissues when these fish were specifically protected from macadamia spray activity and drift by way <strong>of</strong><br />
stocking at a remote site several kilometres away from the Gilson Rd hatchery. This is clear evidence that<br />
spray drift occurs in conjunction with macadamia spray activity and that there are toxic effects in fish caused<br />
by agri‐chemicals contaminating the Gilson Rd hatchery.<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
Page 12 <strong>of</strong> 27
Summary Risk Assessment Statement<br />
The Golden Perch Fish Loss Syndrome at Sunland Fish Hatchery in September 2009 was caused primarily by a<br />
parasitic infection with a protozoan Costia sp. resulting in fish mortalities, with significant secondary toxic<br />
pathological effects in connection to contamination <strong>of</strong> the hatchery by agrichemicals including pesticides such as<br />
nonylphenol, 4‐t‐octylphenol, bisphenol A, carbendazim, and dichlorvos. Toxic pathology in the fish gills, liver<br />
cells and erythrocytes may be specifically attributable to the endocrine disruptor compounds nonylphenol, 4‐t‐<br />
octylphenol and bisphenol A.<br />
Fish that were protected from spray activity did not experience similar toxicological effects and a specific time<br />
relationship <strong>of</strong> the syndrome to the reported spraying activity at the adjacent macadamia plantation is clear<br />
evidence that spray activity and spray drift are the common factors resulting in contamination <strong>of</strong> the hatchery<br />
environment with agrichemicals including pesticides thus producing observable toxicological effects in<br />
unprotected fish.<br />
The recommendations <strong>of</strong> this report are :<br />
A. Provision <strong>of</strong> the spray record information to the veterinary investigation as it is vital to elucidate the exact<br />
sources <strong>of</strong> these agrichemicals with respect to macadamia farming activities.<br />
B. Review <strong>of</strong> the current standards for aquatic toxicity endpoint assessments with respect to delineation <strong>of</strong><br />
actual field exposure scenarios, influence <strong>of</strong> chemical mixtures and toxicity endpoints for native Australian<br />
fish species including the golden perch (Macquaria ambigua) so as to improve the sensitivity and thus<br />
reliability <strong>of</strong> risk assessment standards.<br />
C. Research into the toxicity pathways for nonylphenol, 4‐t‐octylphenol and bisphenol A endocrine disruptors<br />
in fish fry <strong>of</strong> Australian native fish species including M.ambigua, under actual field conditions is required to<br />
elucidate the responses <strong>of</strong> fish to varying conditions <strong>of</strong> exposure. This is necessary to support the<br />
development <strong>of</strong> effective safe practices with respect to agrichemical spray drift risks.<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
Page 13 <strong>of</strong> 27
Australian Bass Growth Retardation Syndrome<br />
1. The Australian Bass Growth Retardation Syndrome (ABGRS) is a significant fish health disorder<br />
impacting the productivity <strong>of</strong> the Sunland Fish Hatchery. ABGRS is now a verifiable condition under the<br />
terms <strong>of</strong> reference <strong>of</strong> the Noosa Fish Health Investigation Task Force (NFHITF).<br />
2. ABGRS manifests itself when fish are reared in a pond subjected to agrichemical spray drift risk during<br />
spray application events by the adjacent macadamia plantation. The period <strong>of</strong> potential exposure in<br />
this study was from 25.11.09 until the 10.2.2010, the period when fish were stocked into the Barra<br />
pond until the time <strong>of</strong> sampling and fish measurements. Evidence <strong>of</strong> contamination <strong>of</strong> the barra pond<br />
specifically and also the hatchery internal environment and other water storages at Sunland Hatchery<br />
(Gilson Rd.) with nonylphenol was established. As nonylphenol has been used by the adjacent<br />
macadamia plantation, the source <strong>of</strong> this agrichemical is likely to be the adjacent macadamia<br />
plantation unless, audited spray record information and environmental sampling and testing <strong>of</strong> the<br />
macadamia plantation show contrary evidence – ie absence <strong>of</strong> use and lack <strong>of</strong> detectable presence on<br />
soil, water or foliage <strong>of</strong> the plantation.<br />
3. ABGRS resulted in a statistically significant (P
The recommendations <strong>of</strong> this report are :<br />
A. To prevent the recurrence <strong>of</strong> Australian Bass Growth Retardation Syndrome (ABGRS), fish must be fully<br />
protected from proximity to all spray activity in time and distance during their entire grow‐out period.<br />
This may be achieved by relocating all grow‐out activities to several kilometres away from the Gilson<br />
road location.<br />
B. Published data on the toxicity <strong>of</strong> nonylphenol agrichemical is not currently available to native<br />
Australian fish species. This makes practical risk assessment currently unreliable and can result in<br />
occurences <strong>of</strong> adverse events such as ABGRS. As a priority, research into the toxicity threshold <strong>of</strong><br />
nonylphenol for native Australian fish species, including the Australian Bass is indicated. To achieve this<br />
end, an Adverse Event <strong>Report</strong> (AER) on nonylphenol to the APVMA is justifiable. Direct tank exposure<br />
trials with known dilutions to levels below current published toxicity endpoints would be useful to<br />
minimise setting no observable toxic effect levels that are too high. Tools for measuring toxic effects<br />
need to be more specific and sensitive, aimed at capturing mechanisms <strong>of</strong> toxicity at the cellular and<br />
sub‐cellular level. Tools such as biochemistry and cell‐line studies are relevant in this regard.<br />
C. Risk modelling <strong>of</strong> spray application scenarios that use the agrichemical nonylphenol as a surfactant or<br />
wetting agent based on actual operational situations <strong>of</strong> application is required to quantity more<br />
accurately the exposure concentrations to fish species. This should replace the current approach <strong>of</strong><br />
grab water samples to estimate exposure, primarily to minimise error <strong>of</strong> underestimation <strong>of</strong> exposure<br />
concentration.<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
Page 15 <strong>of</strong> 27
Silver Perch Larval Malformation,Discoordination and Acute Death Syndrome<br />
1. Silver Perch Larval Malformation, Discoordination and Acute Death (SPMDAD) Syndrome is a complex<br />
fish health disorder within the scope <strong>of</strong> syndromes described by the Noosa Fish Health Investigation<br />
Task Force. Although there is insufficient pathological data to study the development <strong>of</strong> SPMDAD in<br />
detail, this epidemiological and toxicological assessment provided a necessary starting point. It is a<br />
significant fish health disorder within the context <strong>of</strong> agrichemical spray drift risk assessment and<br />
mitigation.<br />
2. SPMDAD is a toxin related and acute condition. This is supported by the observation that activated<br />
charcoal filtration <strong>of</strong> spawning tank water improved the survival rate <strong>of</strong> affected fish larvae. SPMDAD<br />
causes severe morbidity and mortality <strong>of</strong> affected fish resulting in estimated rates <strong>of</strong> mortality in<br />
fertilised fish embryos <strong>of</strong> 99%, <strong>of</strong> malformation in embryos <strong>of</strong> 25% and <strong>of</strong> swimming discoordination in<br />
hatched fish larvae <strong>of</strong> 65%.<br />
3. Spray drift contamination occurred through a breach in the hatchery spray drift biosecurity program by<br />
the opening <strong>of</strong> the hatchery door in the evenings and through lack <strong>of</strong> covering over water storage<br />
tanks. This contributed to the contamination <strong>of</strong> the hatchery air environment and potentially water<br />
used for fish spawning with trichlorphon/dichlorvos and nonylphenol. Both agrichemicals have been<br />
reported to be used recently by the macadamia plantation. However, the actual exposure<br />
concentration to the affected fish eggs and larvae are not known due to lack <strong>of</strong> water samples from the<br />
hatchery tanks.<br />
4. Nonylphenol has been shown to cause lethal and non‐lethal fish embryonic deformities. Nonylphenol<br />
has been shown to cause hatching failures in Australian native fish. Nonylphenol exposure may explain<br />
the malformation and hatching failure observed. Trichlorphon/dichlorvos are agrichemicals with<br />
neurological toxicities which may explain the swimming discoordination observed. A combination <strong>of</strong><br />
toxicities by nonylphenol and trichlorphon/dichlorvos may result in acute death <strong>of</strong> fish embryos and<br />
larvae.<br />
5. Definitive studies on the toxicity <strong>of</strong> nonylphenol and trichlorphon/dichlorvos are indicated to provide<br />
risk mitigation information in relation to agrichemical spray drift scenarios for fish hatchery operations.<br />
Summary Risk Assessment Statement<br />
Silver Perch Larval Malformation, Discoordination and Acute Death (SPMDAD) Syndrome can be<br />
explained by agrichemical spray drift contamination into a hatchery environment involving nonylphenol<br />
and trichlorphon/dichlorvos.<br />
The issue <strong>of</strong> uncertainty is with respect to the actual exposure scenarios and the exposure<br />
concentrations at which silver perch fish eggs, embryos and fish larvae would not experience SPMDAD.<br />
The recommendations <strong>of</strong> this report are :<br />
A. Spray drift protection measures in a hatchery environment should consider the following risk factors :<br />
o Opening <strong>of</strong> the door <strong>of</strong> the hatchery at night time even in the absence <strong>of</strong> spray activities at night. Day<br />
time spray residue may still be present in the air for an undeterimined period depending on wind<br />
and temperature conditions as well as agrichemical behaviour. Therefore the hatchery door should<br />
be closed with only very short periods <strong>of</strong> opening for ventilation and other activities.<br />
o All water storage tanks inside the hatchery can be contaminated if the concentration in the air <strong>of</strong><br />
agrichemical spray droplets is high enough. Therefore tank covers may mitigate the risk <strong>of</strong><br />
contamination in this situation.<br />
o Use and replenishment <strong>of</strong> activated charcoal filtration <strong>of</strong> water used in spawning tanks should be a<br />
standard preventative procedure as long as it is applied to treat water prior to exposure <strong>of</strong> fish eggs<br />
to potentially contaminated water.<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
Page 16 <strong>of</strong> 27
B. Nonylphenol, though not a pesticide, is an agrichemical commonly used in the delivery <strong>of</strong> pesticides. It<br />
appears to have a potentially wide range <strong>of</strong> toxicities in fish including endocrine disruption, growth<br />
retardation, oxidative stress stimulation, embryonic malformations and acute death. Although published<br />
toxicity endpoints for various effects suggest a high tolerable concentration ( tens <strong>of</strong> g to several mg/L),<br />
whether the combined toxic mechanisms <strong>of</strong> this agrichemical when expressed in susceptible species <strong>of</strong> fish<br />
could result in endpoint toxicities several orders <strong>of</strong> magnitude less than the individual toxicity endpoints<br />
needs to be thoroughly researched and investigated<br />
C. Until such time when specific risk mitigation information from (B) is available, from a fish health protection<br />
point <strong>of</strong> view, avoidance <strong>of</strong> any exposure to nonylphenol is recommended.<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
Page 17 <strong>of</strong> 27
Supporting Documents<br />
Date Title<br />
Content pr<strong>of</strong>ile<br />
14.11.08 Biosecurity Sciences Laboratory (BSL)<br />
Test results on silver perch, golden perch and<br />
Supplementary pathology report 08‐178975 Australian bass samples<br />
31.10.08 Chemical residue report 6134 Test results on silver perch, golden perch<br />
samples<br />
14.11.08 BSL pathology report 08‐180386 Test results on chicken samples<br />
13.11.08 Chemical residue report 6147 Test results on chicken samples<br />
13.11.08 BSL pathology report 08‐180<strong>37</strong>1 Test results on horse samples<br />
13.11.08 Chemical residue report 6148 Test results on horse samples<br />
BSL pathology report 08‐179890 Test results on silver perch samples<br />
16.12.08 Queensland Health Forensic and Scientific Services<br />
(QHFSS) analytical report 08NA12582‐08NA12583 :<br />
XY<br />
Test results on water samples<br />
14.1.09 Chemical residue report 6198 Test results on water sample<br />
21.1.09 Chemical residue report 6200 Test results on silver perch sample<br />
18.2.09 QHFSS anaysis report 08PE328‐330:UT Test results on silver perch samples<br />
21.1.09 Chemical residue report 6201 Test results on silver perch samples<br />
14.1.09 Chemical residue report 6199 Test results on water sample<br />
20.3.09 BSL pathology report 08‐182660 Test results on silver perch samples<br />
5.2.09 BSL pathology report 08‐184972 Test results on silver perch samples<br />
21.1.09 Chemical residue report 6203 Test results on silver perch samples<br />
20.3.09 BSL pathology report 08‐184980 Test results on silver perch samples<br />
21.1.09 Chemical residue report 6202 Test results on silver perch samples<br />
20.3.09 BSL pathology report 08‐185511 Test results on silver perch samples<br />
27.1.09 Chemical residue report 6204 Test results on silver perch sample<br />
20.3.09 BSL pathology report 08‐185511 Test results on silver perch samples<br />
20.3.09 BSL pathology report 09‐103042 Test results on silver perch, water lily and<br />
sediment samples<br />
20.3.09 BSL pathology report 09‐103050 Test results on silver perch samples<br />
20.3.09 BSL pathology report 09‐103692 Test results on feed and mosquito fish<br />
samples<br />
20.3.09 BSL pathology report 09‐10<strong>37</strong>03 Test results on tadpole samples<br />
20.3.09 BSL pathology report 09‐103951 Test results on silver perch samples<br />
20.3.09 BSL pathology report 09‐103966 Test results on golden perch samples<br />
20.3.09 BSL pathology report 09‐103943 Test results on Australian bass samples<br />
15.12.08 Sunland Fish Hatchery – Farm Inspection and Results <strong>of</strong> farm inspection and record <strong>of</strong><br />
Sampling 9.12.08 <strong>Report</strong><br />
sampling<br />
6.12.08 Sunland Fish Hatchery <strong>Veterinary</strong> Investigation<br />
Work Instruction Version 4/4<br />
<strong>Veterinary</strong> investigation work strategy<br />
20.3.09 Chemical residue report 6252 Cyfluthrin repeat at LOR 1ppb 08‐184980<br />
20.3.09 Chemical residue report 6253 Cyfluthrin at LOR 1ppb 09‐103042<br />
20.3.09 Chemical residue report 6254 Test results on sediment samples<br />
20.3.09 Chemical residue report 6255 Test results on mosquito fish and feed<br />
sample<br />
20.3.09 Chemical residue report 6256 Test results on cane toad tadpoles<br />
8.10.09 Spray Record Transcripts Appendix 1,13,14,16,18 Macadamia farm pesticide spray information<br />
15.7.09 BSL Pathology <strong>Report</strong> 09‐119795<br />
Mullet pathology<br />
29.7.09 BSL Pathology <strong>Report</strong> 09‐120470<br />
5.3.2010 Queensland Health Scientific and Forensic Services<br />
Analytic <strong>Report</strong> Nos.<br />
Residue results on mullet samples<br />
22.10.09<br />
SSP0021779,21797‐10KE1258‐1264,1328‐1330<br />
(09‐120470)<br />
SSP0021781‐10KE1277‐1279 (09‐121094)<br />
Biosecurity Sciences Pathology <strong>Report</strong> 09‐126853 Bass fry pathology<br />
19.10.09 Queensland Health Scientific and Forensic Services<br />
Analytic <strong>Report</strong> No. 09KE6807‐09KE6809 : PW1<br />
Residue results on water samples – Bass fry<br />
1.12.09 Biosecurity Sciences Pathology <strong>Report</strong>s Golden perch pathology<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
Page 18 <strong>of</strong> 27
14.12.09<br />
10.2.2010<br />
6.1.2010<br />
09‐132811<br />
09‐132792<br />
09‐132776<br />
09‐132803<br />
09‐132784<br />
09‐132761<br />
10‐102971<br />
Queensland Health Scientific and Forensic Services<br />
Analytic <strong>Report</strong> Nos.<br />
09KN228‐09KE8072:SC2<br />
09KE8064‐8701:SBC SSP20561<br />
SSP0020561‐09KE8072<br />
2009 Sunland Fish Hatchery Spray Drift Protection<br />
Program<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
Page 19 <strong>of</strong> 27<br />
Residue results on water and filter paper<br />
samples<br />
Hatchery spray protection procedures<br />
4.3.2010 Biosecurity Sciences Pathology <strong>Report</strong>s<br />
Bass pathology<br />
10 ‐ 102963<br />
Queensland Health Scientific and Forensic Services<br />
Analytic <strong>Report</strong> Nos.<br />
Residue results on water samples<br />
17.2.1010 09KE8830‐8834,09KE8836:SBC SSP21044<br />
5.3.2010<br />
09KE8835‐SSP0021044<br />
3.2010 Biosecurity Sciences Pathology <strong>Report</strong><br />
Silver perch larvae pathology<br />
10‐103510<br />
4.3.2010 Chemical Residue <strong>Report</strong> 6662 Soil sample residue results<br />
5.3.2010 Queensland Health Scientific and Forensic Services<br />
Analytic <strong>Report</strong> Nos.<br />
Residue result on filter paper sample<br />
SSP0021044 – 09KE8835<br />
20.3.09 1 st <strong>Veterinary</strong> <strong>Report</strong> – Silver Perch Kill, Bass<br />
Deformity and Neurologic Syndromes 1st Edition<br />
<strong>Veterinary</strong> investigation report<br />
12.5.09 1 st <strong>Veterinary</strong> <strong>Report</strong> – Silver Perch Kill, Bass <strong>Veterinary</strong> investigation report – Peer<br />
Deformity and Neurologic Syndromes 2nd Edition Reviewed<br />
16.10.09 1 st <strong>Veterinary</strong> <strong>Report</strong> – Silver Perch Kill, Bass<br />
Deformity and Neurologic Syndromes 3 rd <strong>Veterinary</strong> investigation report –<br />
Edition incorporating spray drift model and spray log<br />
information<br />
29.7.09 2 nd <strong>Veterinary</strong> <strong>Report</strong> – Mullet Defprmities<br />
Syndrome 1 st Edition<br />
<strong>Veterinary</strong> investigation report<br />
7.4.2010 2 nd <strong>Veterinary</strong> <strong>Report</strong> – Mullet Defprmities<br />
Syndrome 2 nd <strong>Veterinary</strong> investigation report –<br />
Edition<br />
incorporating residue results, environmental<br />
data and statistical analysis<br />
23.10.09 3 rd <strong>Veterinary</strong> <strong>Report</strong> ‐ Bass Fry Kill Syndrome <strong>Veterinary</strong> investigation report<br />
11.2.2010 4 th <strong>Veterinary</strong> <strong>Report</strong> ‐ Golden Perch Loss<br />
Syndrome<br />
<strong>Veterinary</strong> investigation report<br />
6.3.2010 5 th <strong>Veterinary</strong> <strong>Report</strong> ‐ Australian Bass Growth<br />
Retardation Syndrome<br />
<strong>Veterinary</strong> investigation report<br />
6.3.2010 6 th <strong>Veterinary</strong> <strong>Report</strong> ‐ Silver Perch Larval<br />
Malformation,Discoordination and Acute Death<br />
Syndrome<br />
<strong>Veterinary</strong> investigation report
Literature<br />
40 CFR Part 180 Methoxyfenozide; Pesticide Tolerances and Time‐Limited Pesticide Tolerances<br />
http://www.epa.gov/fedrgstr/EPA‐PEST/2008/March/Day‐05/p4027.pdf<br />
Aceret,T.L., Sammarco, P.W. and Coll,, J.C. (2001) Discrimination between several diterpenoid compounds in<br />
feeding by Gambusia affinis. Comparative Biochemistry and Physiology Part C : Toxicology and Pharmacology.<br />
Vol. 128, Issue 1, pp. 55‐63.<br />
Andreasen, J.K. (1985). Insecticide resistance in mosquito fish <strong>of</strong> the Lower Rio Grande valley <strong>of</strong> Texas – An<br />
ecological hazard ? Archives <strong>of</strong> Environmental Contamination and Toxicology, Vol 14, No.5 pp. 573‐577.<br />
Agency for Toxic Substances and Disease Registry (ATSDR) Public Health Assessments and Consultations Fish and<br />
Shellfish Evaluation ‐ Isla De Vieques Bombing Range, Vieques, Puerto Rico (2003).<br />
http://www.atsdr.cdc.gov/HAC/PHA/viequesfish/viequespr‐toc.html and<br />
http://www.atsdr.cdc.gov/HAC/PHA/viequesfish/viequespr‐p3.html<br />
Arellano, J.M.,Ortiz, J.B.,Capeta Da Silva, D.,González de Canales, M.L.,Sarasquete, C. and Blasco, J. (1999).<br />
Levels <strong>of</strong> copper, zinc, manganese and iron in two fish species from salt marshes <strong>of</strong> Cadiz Bay (southwest Iberian<br />
Peninsula) Bol. Inst. Esp. Oceanogr. 15 (1‐4). 1999: 485‐488.<br />
Arsenault, J.T.M., Fairchild, W.L. D.,MacLatchy,L.,Burridge,L.,Haya. K.and Brown, S.B. (2004). Effects <strong>of</strong> water‐<br />
borne 4‐nonylphenol and 17β‐estradiol exposures during parr‐smolt transformation on growth and plasma IGF‐I<br />
<strong>of</strong> Atlantic salmon (Salmo salar L.). Aquatic Toxicology,Volume 66, Issue 3, 25 February 2004, Pages 255‐265.<br />
Australian and New Zealand Environment and Conservation Council (ANZECC) Guidelines for Fresh and Marine<br />
Water Quality – Chapter 9.4 : Aquaculture and human consumers <strong>of</strong> aquatic food. (2000)<br />
http://www.mincos.gov.au/__data/assets/pdf_file/0020/316145/gfmwq‐guidelines‐vol3‐9‐4.pdf<br />
Balasubramanian, P. Saravanan, T.S., and Palaniappan, M.K. (1999). Biochemical and Histopathological Changes<br />
in Certain Tissues <strong>of</strong> Oreochromis mossambicus (Trewaves) Under Ambient Urea Stress. Bull. Environ. Contam.<br />
Toxicol. (1999) 63:117‐124.<br />
Bhattacharya, H., Xiao, Q. and Lun, L. (2008). Toxicity studies <strong>of</strong> nonylphenol on rosy barb (Puntius conchonious):<br />
A biochemical and histopathological evaluation Tissue and Cell. Volume 40, Issue 4, August 2008, Pages 243‐249.<br />
Birge,W.J.,Black, J.A., Westerman, A.G. and Ramey,B.A. (1983). Fish and amphibian embryos – a model system for<br />
evaluating teratogenicity. Toxicological Sciences, Vol. 3, Number 4. Pp. 2<strong>37</strong>‐242.<br />
Carlisle, J. and D. Roney. 1984. Bioconcentration <strong>of</strong> cyfluthrin (Baythroid) by bluegill sunfish. Mobay Chemical<br />
Corp. <strong>Report</strong> No. 86215<br />
Carmago, M.M.P and Martinez, C.B.R. (2007). Histopathology <strong>of</strong> gills, kidney and liver <strong>of</strong> a Neotropical fish caged<br />
in an urban stream. Neotropical Ichthyology, 5(3): 327‐336, 2007.<br />
Carson, R. 1962. Silent Spring. Houghton Mifflin Publishers, Boston, USA; 368 p.<br />
Cech, J.J., Massingill, M.J., Voncracek, B. and Linden, A.L. (1985). Respiratory metabolism <strong>of</strong> mosquit<strong>of</strong>ish,<br />
Gambusia affinis : effects <strong>of</strong> temperature, dissolved oygen and sex difference. Environmental Biology <strong>of</strong> Fishes,<br />
Vol.13, No.4 pp. 297‐307.<br />
Chambers, J.E. and Yarbrough, D. (1979). A seasonal study <strong>of</strong> microsomal mixed‐function oxidase components in<br />
insecticide‐resistant and susceptible mosquit<strong>of</strong>ish, Gambusia affinis. Toxicology and Applied Pharmacology, Vol.<br />
48, Issue 3, pp. 497‐507.<br />
Cox, G.W. 1997. Conservation Biology ‐ 2nd ed. WCB.<br />
<strong>Department</strong> <strong>of</strong> <strong>Primary</strong> <strong>Industries</strong> and Fisheries (DPI&F) Grouper Metals Test Results <strong>Report</strong> 08PE29‐31:UT<br />
(2008).<br />
Di Giulio, R.T. and Hinton, D.E. (2008) Toxicology <strong>of</strong> Fishes. CRC Taylor and Francis Press.<br />
Extoxnet (1996). Extension Toxicology Network Pesticide Information Pr<strong>of</strong>iles – Dichlorvos.<br />
http://extoxnet.orst.edu/pips/dichlorv.htm<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
Page 20 <strong>of</strong> 27
Ferguson, H.W. (2006). Systemic Pathology <strong>of</strong> Fish, A Text and Atlas <strong>of</strong> Normal Tissues in Teleosts and their<br />
Responses in Disease.<br />
FAO (2003). http://www.inchem.org/documents/jmpr/jmpmono/v2003pr07.htm<br />
Ferguson, H.W. (2006) Systemic Pathology <strong>of</strong> Fish – A Text and Atlas <strong>of</strong> Normal Tissues in Teleosts and their<br />
Responses in Disease. Scotian Press.<br />
Food Standards Australia New Zealand (FSANZ) (2005). Final Assessment <strong>Report</strong>, Proposal P265, <strong>Primary</strong><br />
Production and Processing Standard for Seafood.<br />
http://www.foodstandards.gov.au/_srcfiles/P265_Seafood_PPPS_FAR.pdf<br />
Food Standards Australia New Zealand (FSANZ 2008). 22 nd Australian Total Diet Study.<br />
http://www.foodstandards.gov.au/_srcfiles/ATDS_App5.pdf<br />
Frasco,M.F., Fournier, D., Carvalho, F. and Guilhermino, L. (2005). Do metals inhibit acetylcholinesterase (AChE)?<br />
Implementation <strong>of</strong> assay conditions for the use <strong>of</strong> AChE activity as a biomarker <strong>of</strong> metal toxicity. Biomarkers<br />
Vol.10, Issue 5, Sep. 2005. pp. 360‐<strong>37</strong>5.<br />
Gilbert, J.J. (1994). Susceptibility <strong>of</strong> planktonic rotifers to a toxic strain <strong>of</strong> Anabaena flos‐aquae. Limnol.<br />
Oceanogr., 39(6), 1994, 1286‐1297.<br />
Galembeck, E., Alonso, A. and Meirelles, N.C., (1998). Effects <strong>of</strong> polyethylene chain lenght on erythrocyte<br />
hemolysis induced by poly [oxyethylene (n) nonylphenol] nonionic surfactants. Chem. Biol. Interact. 113 2, pp.<br />
91–103.<br />
Gordon, C.J. (2005) Temperature and toxicology: an integrative, comparative, and environmental approach.<br />
Published by CRC Press, 2005 pp. 235 – 240.<br />
Grabow,W.O.K., Du Randt, W.C., Prozesky, O.W. and Scott, W.E. (1982). Microcystis aeruginosa Toxin: Cell<br />
Culture Toxicity,Hemolysis, and Mutagenicity Assays. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. June<br />
1982, p. 1425‐1433 Vol. 43, No. 6<br />
Gray,M.A. and Metcalfe, C.D. (1999). Toxicity <strong>of</strong> 4‐tert‐octylphenol to early life stages <strong>of</strong> Japanese medaka<br />
(Oryzias latipes). Aquatic Toxicology 46 (1999) 149‐154.<br />
Green, J.M. (1999). Effect <strong>of</strong> Nonylphenol Ethoxylation on the Biological Activity <strong>of</strong> Three Herbicides with<br />
Different Water Solubilities. Weed Technology, 1999, Volume 13:840‐842.<br />
Halliwell, B. (1997). Antioxidants and human diseases: a general introduction. Nutr Rev 55:44‐52.<br />
Harford, A.J., O’Halloran, K., and wright, P.F.A. (2005). The effects <strong>of</strong> in vitro pesticide exposures on the<br />
phagocytic function <strong>of</strong> four native Australian freshwater fish. Aquatic Toxicology 75 (2005) pp.330‐342.<br />
Harris, J.H. (1987). Growth <strong>of</strong> Australian bass Macquaria novemaculeata (Perciformes : Percichthyidae)<br />
in the Sydney Basin. Aust. J. Mar. Freshw. Res., 1987, 38, 351‐61.<br />
Heath, A.G. (1995) Water Pollution and Fish Physiology. CRC Lewis Publishers. pp. 334<br />
Heath, S. Bennett,W.A., Kennedy,J. and Beitinger, T.L. 1994 Heat and cold tolerance <strong>of</strong> the fathead minnow,<br />
Pimephales promelas, exposed to the synthetic pyrethroid cyfluthrin. Can.J.Fish.Aquat.Sci. 51, 4<strong>37</strong>‐440.<br />
Heath, A.G. 1998. Physiology and ecological health. In Cech, J.J., Wilson, B.W., Crosby, D.G.,<br />
eds, Multiple Stresses in Ecosystems. Lewis Publishers, Washington, DC, USA, pp 59‐89.<br />
Heimbach, F., W. Pflueger, and Ratte, H. 1992. Use <strong>of</strong> small artificial ponds for assessment <strong>of</strong> hazards to aquatic<br />
ecosystems. Environmental Toxicology and Chemistry, 11:27‐34.<br />
H<strong>of</strong>fmann, R.W., Stolle, A., Eisgruber, H. and Kölle, P. (1995). Clostridium bifermentans infection in grass carp<br />
(Ctenopharyyngodon idella). Berl.Munch.Tierarztl.Wschr. 108,55‐57 (1995).<br />
Holdway, D.A., Hefferman, J. and Smith, A. (2008). Multigeneration assessment <strong>of</strong> nonylphenol and endosulfan<br />
using a model Australian freshwater fish, Melanotaenia fluviatilis. Environmental Toxicology and Water Quality<br />
Volume 23 Issue 2, Pages 253 – 262.<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
Page 21 <strong>of</strong> 27
JMPR (2003).<br />
http://www.fao.org/ag/AGP/AGPP/Pesticid/JMPR/Download/2003_eva/methoxyfenozide%202003.pdf<br />
Kadomura, K.,Naruse, S. ,Sugihara, S.,Yamaguchi, K. and Oda, T. (1992). Production <strong>of</strong> reactive oxygen species<br />
(ROS) by various marine fish species during the larval stage Japan Society for Bioscience Biotechnology and<br />
Agrochemistry, Tokyo, JAPON (1992) (Revue).<br />
Kammann, U., Vobach, M., Wosniok, W., Schäffer, A. and Telscher, A. (2009). Acute toxicity <strong>of</strong> 353‐nonylphenol<br />
and its metabolites for zebrafish embryos . Environmental Science and Pollution Research ‐ Environ Sci Pollut Res<br />
(2009) 16:227–231.<br />
Kunaraguru, A.K. and Beamish, F.W.H. (1983) Bioenergetics <strong>of</strong> acclimation to permethrin (NRDC‐143) by rainbow<br />
trout, Comp. Biochem. Physiol., 75A, 247.<br />
Langdon, J.S. and Nowak, B.F. (1992). Pollutants and Biotoxins in Fin Fish Workshop. Refresher Course for<br />
Veterinarians. Proceedings 182. Post Graduate Committee in <strong>Veterinary</strong> Science, University <strong>of</strong> Sydney.<br />
Langdon, J.S. (1992). Major Parasitic diseases <strong>of</strong> Australian Finfish in Fin Fish Workshop. Refresher Course for<br />
Veterinarians. Proceedings 182. Post Graduate Committee in <strong>Veterinary</strong> Science, University <strong>of</strong> Sydney.<br />
Langdon, J.S. (1988). History and causes <strong>of</strong> fish kills. Post Graduate Committee in <strong>Veterinary</strong> Science. Proceedings<br />
106. pp.190.<br />
Lanno,R.P., Hickie, B.E. and Dixon, D.G. (2004) Feeding and nutritional considerations in aquatic toxicology.<br />
Journal <strong>of</strong> Hydrobiologia, Vol. 188‐189, No.1 pp. 525‐531.<br />
Lee‐Steere,C. (2009). <strong>Report</strong> to Australian Pesticides and <strong>Veterinary</strong> Medicines Authority – Noosa Fish Health<br />
Incident ‐ Ecotoxicological Endpoints for Fish and other aquatic organisms. Chemical Review Work Order CR294‐<br />
1.<br />
Little, E.E., Finger, S.E. 1990. Swimming behavior as an indicator <strong>of</strong> sublethal toxicity in fish.<br />
Environmental Toxicology and Chemistry, 9:13‐19.<br />
Mackay, N.J., Bebbington, G.N., Chvojka, R.,Williams, R.J., Dunn, A. and Auty, E.H. (1977). Heavy Metals,<br />
Selenium and Arsenic in Nine Species <strong>of</strong> Australian Commercial Fish Aust. J. Mar. Freshwater Res., 1977, 28, 277‐<br />
86.<br />
McMillan,D.B. (2007). Fish Histology – Female Reproductive Systems. Springer. Pp. 72.<br />
Maseda, M. El‐Gharabawy and Samira S. Assem (2006). Spawning induction in the Mediterranean grey mullet<br />
Mugil cephalus and larval developmental stages. African Journal <strong>of</strong> Biotechnology Vol. 5 (19), pp. 1836‐1845, 2<br />
October, 2006.<br />
Mills,L.J.,Gutjahr‐Gobell,R.E.,Haebler,R.A., Borsay Horowitz,D.J.,Jayaraman,S., Pruell,R.J., McKinney,R.A.,<br />
Gardner,G.R. and Zaroogian,G.E. (2001). Effects <strong>of</strong> estrogenic (o,p ‐DDT; octylphenol) and anti‐androgenic (p,p ‐<br />
DDE) chemicals on indicators <strong>of</strong> endocrine status in juvenile male summer flounder (Paralichthys dentatus)<br />
Aquatic Toxicology Volume 52, Issue 2, April 2001, Pages 157‐176.<br />
Munday, B.L. (1990) Fin Fish Diseases, Proceedings 128. Post Graduate Committee in <strong>Veterinary</strong> Science,<br />
University <strong>of</strong> Sydney.<br />
Nakanishi,J., Miyamoto,K‐I, and Kawasaki, H. (2007). Bisphenol A Risk Assessment Document (AIST Risk<br />
Assessment Document Series No. 4) Summary.<br />
http://unit.aist.go.jp/riss/crm/mainmenu/BPA_Summary_English.pdf<br />
Navarro, B., Nunes, B., Varó, I. and Guilhermino, L. (2007). Effects <strong>of</strong> dichlorvos aquaculture treatments on<br />
selected biomarkers <strong>of</strong> gilthead sea bream (Sparus aurata L.) fingerlings. Aquaculture<br />
Volume 266, Issues 1‐4, 1 June 2007, Pages 87‐96.<br />
Noga, E.J. (1996). Fish Disease Diagnosis and Treatment. Mosby Year Book.<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
Page 22 <strong>of</strong> 27
NRA (2002). Public Release Summary on Evaluation <strong>of</strong> the new active METHOXYFENOZIDE in the product<br />
PRODIGY 240 SC INSECTICIDE National Registration Authority for Agricultural and <strong>Veterinary</strong> Chemicals<br />
http://www.apvma.gov.au/publications/downloads/prs_methoxyfenozide.pdf<br />
Oates, D. and Stucky, N. (1977). Preliminary Survey <strong>of</strong> Heavy Metal Contamination <strong>of</strong> Channel Catfish in<br />
Nebraska. Nebraska Game and Parks Commission Nebraska Game and Parks Commission – Staff Research<br />
Publications, University <strong>of</strong> Nebraska ‐ Lincoln Year 1977.<br />
http://digitalcommons.unl.edu/cgi/viewcontent.cgi?article=1013&context=nebgamestaff<br />
Okai, Y., Sato, E.F., Higashi‐Okai, K. and Inoue, M. (2004). Enhancing Effect <strong>of</strong> the Endocrine Disruptor para‐<br />
Nonylphenol on the Generation <strong>of</strong> Reactive Oxygen Species in Human Blood Neutrophils. Environmental Health<br />
Perspectives Volume 112, Number 5, April 2004.<br />
Oros, Daniel R. and Inge Werner. 2005. Pyrethroid Insecticides: An Analysis <strong>of</strong> Use Patterns,<br />
Distributions, Potential Toxicity and Fate in the Sacramento‐San Joaquin Delta and Central Valley. White Paper<br />
for the Interagency Ecological Program. SFEI Contribution 415. San Francisco Estuary Institute, Oakland, CA.<br />
Pan Pesticide Database – Methoxyfenozide http://www.pesticideinfo.org/Detail_Chemical.jsp?Rec_Id=PC<strong>37</strong>414<br />
PAN Pesticide Database – Toxicity Studies for Cyfluthrin on Fish.<br />
http://www.pesticideinfo.org/List_AquireAll.jsp?Rec_Id=PC33504&Taxa_Group=Fish<br />
PAN Pesticides Database – Chemical Toxicity Studies on Aquatic Organisms – Toxicity Studies for Urea on Fish.<br />
http://www.pesticideinfo.org/List_AquireAll.jsp?Rec_Id=PC35157&Taxa_Group=Fish<br />
Park,J‐H,Kim, D‐J,Seok, S‐H, Baek,M‐W,Lee, H‐Ya, Na, Y‐R, Park,S‐H,Lee,H‐Ka,Dutta,N.K., and Kawakamib,K.<br />
(2008). Benomyl induction <strong>of</strong> brain aromatase and toxic effects in the zebrafish embryo Journal <strong>of</strong> applied<br />
toxicology 2009, vol. 29, n o 4, pp. 289‐294.<br />
Patra, R.W., Chapman, J.C., Lim, R. and Gehrke, P.C. (2007). The effects <strong>of</strong> three organic chemicals on the upper<br />
thermal tolerances <strong>of</strong> four freshwater fishes. Environmental Toxicology and Chemistry, Vol. 26, No. 7, pp. 1454‐<br />
1459.<br />
Payne, R.W., Harding, S.A., Murray, D.A., Soutar, D.M., Baird, D.B., Welham, S.J., Kane, A.F., Gilmour, A.R.,<br />
Thompson, R., Webster, R., & Tunnicliffe Wilson, G. (2007). The Guide to GenStat Release 10, Part 2: Statistics.<br />
VSN International, Hemel Hempstead.<br />
Pesticides Properties DataBase (PPDB) http://sitem.herts.ac.uk/aeru/iupac/461.htm<br />
Pfeil, R.and Dellarco,V. (2005) Carbendazim 87‐106 The Joint FAO/WHO Meeting on Pesticide Residues (JMPR)<br />
2005.<br />
Pierce, R.H. and Henry, M.S. (2008). Harmful algal toxins <strong>of</strong> the Florida red tide (Karenia brevis): natural chemical<br />
stressors in South Florida coastal ecosystems. Ecotoxicology. 2008 October; 17(7): 623–631.<br />
Poleo, G.A., Denniston, R.S., Reggio, B.C., Godke, R.A. and Tiersch, T.R. (2001). Fertilization <strong>of</strong> eggs <strong>of</strong> Zebrafish,<br />
Danio rerio, by intracytoplasmic sperm injection. Biology <strong>of</strong> Reproduction 65, 961‐966 (2001).<br />
Read, P., Landos, M., Rowland, S. Mifsud, C. (2007) Diagnosis, treatment & Prevention <strong>of</strong> the Diseases <strong>of</strong> the<br />
Australian Freshwater Fish Silver Perch (Bidyanus bidyanus) NSW DPI.<br />
Reddy, P.M., Harold‐Philip, G. and Bashamohideen, Md. (1992). Regulation <strong>of</strong> AChE System <strong>of</strong> Freshwater Fish,<br />
Cyprinus carpio, under Fenvalerate Toxicity. Bull. Environ. Contam. Toxicol. (1992) 48:18‐22.<br />
Roberts, R.J. (2001) Fish Pathology 3 rd edition. WB Saunders.<br />
Robertson and Hansen (2001). PCBs – Recent Advances in Environmental Toxicology and Health Effects,<br />
University Press <strong>of</strong> Kentucky. Pp. 273‐274.<br />
Rodgers, J.H. Jr (2008). Algal Toxins in Pond Aquaculture. SRAC Publication No. 4605<br />
http://www.ca.uky.edu/wkrec/AlgalToxinsPond.pdf<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
Page 23 <strong>of</strong> 27
Rowland, J.R. and Bryant, C. (1994), Silver Perch Culture Proceedings <strong>of</strong> Silver Perch Aquaculture Workshops,<br />
Grafton and Narrandera.<br />
Rowland S.J., Landos, M., Callinan, R.B., Allan, G.L., Read, P., Mifsud, C., Nixon, M., Boyd, P. and Tully, P. (2007)<br />
Development <strong>of</strong> a Health Management Strategy for the Silver Perch Aquaculture Industry. NSW DPI – Fisheries<br />
Final <strong>Report</strong> Series No. 93 ISSN 1449‐9967.<br />
Rodrigues, E de L. and Fanta, E. (1998). Liver histopathology <strong>of</strong> the fish Brachydanio rerio (Hamilton‐Buchman)<br />
after acute exposure to sublethal levels <strong>of</strong> the organophosphate dimethoate 500. Revta bras. Zool. 15 (2) : 441‐<br />
450, 1998.<br />
Sahn SC. (1991). Role <strong>of</strong> oxygen free radicals in the molecular mechanism <strong>of</strong> carcinogenesis. J Environ Sci Health<br />
9:83‐112.<br />
Santerre, C.R., Bush, P.B., Xu, D.H., Lewis, G.W., Davis, J.T., Grodner, R.M., Ingram, R., Wei, C.I., Hinshaw, J.M.<br />
(2001). Metal Residues in Farm‐Raised Channel Catfish, Rainbow Trout, and Red Swamp Crayfish from the<br />
Southern U.S. Journal <strong>of</strong> Food Science —Vol. 66, No. 2, 2001.<br />
Schwaiger, J., O. H Spieser, O.H., Bauer, C., H. Ferling, C.H., U. Mallow, U., W. Kalbfus, W. and R. D. Negele, R.D.<br />
(1998). Chronic toxicity <strong>of</strong> nonylphenol and ethinylestradiol: haematological and histopathological effects in<br />
juvenile Common carp (Cyprinus carpio) Aquatic Toxicology Volume 51, Issue 1, November 2000, Pages 69‐78.<br />
Sclenk,D. and Benson, W.H. (2001). Targen Organ Toxicity in Marine and Freshwater Teleosts. Volume 2 –<br />
Systems. Taylor and Francis.<br />
Selvi, M., Sarikaya, R.,Erkoc, R.F. and Koc, O. (2008). Acute toxicity <strong>of</strong> the cyfluthrin pesticide on guppy fish.<br />
Environ Chem Lett DOI 10.1007/s10311‐008‐0142‐5.<br />
Shaw, B.P. and Panigrahi, A.K. (1990). Brain AChE activity studies in some fish species collected from a mercury<br />
contaminated estuary. Water, Air and Soil Pollution 53:327‐334, Kluwer Academic Publishers.<br />
Smith, C.E. 1979, The prevention <strong>of</strong> liver lipoid degeneration (ceroidosis) and microcytic anaemia in rainbow<br />
trout Salmo gairdneri Richardson fed rancid diets: a preliminary report, J. Fish. Dis. 2 (1979), pp. 429–4<strong>37</strong>.<br />
Smith, M.E. and Belk, M.C. (2001) Risk assessment in western mosquitifish (Gambusia affinis) : do multiple cues<br />
have additive effects ? Behav. Ecol. Sociobiol (2001) 51:101‐107.<br />
Solecki Roland , Les Davies, Vicki Dellarco c, Ian Dewhurst, Marcel van Raaij, Angelika Tritscher, (2005). Guidance<br />
on Setting <strong>of</strong> Acute Reference Dose (ARfD) for Pesticides, Food and Chemical Toxicology 43 (2005) 1569–1593.<br />
Stoskopf, M.K. (1993). Fish Medicine. WB Saunders.<br />
Şükriye ArasHisar, Olcay Hisar, Telat Yanık and Sıtkı M. Aras (2004). Inhibitory effects <strong>of</strong> ammonia and urea on gill<br />
carbonic anhydrase enzyme activity <strong>of</strong> rainbow trout (Oncorhynchus mykiss) Environmental Toxicology and<br />
Pharmacology Volume 17, Issue 3, July 2004, Pages 125‐128<br />
Takao, Y., Oishi, M., Nagae, M., Kohra, S. and Arizono, K. (2008). Bisphenol A Incorporated into Eggs from Parent<br />
Fish Persists for Several Days. Journal <strong>of</strong> Health Science, 54(2), 235‐239 (2008).<br />
Talbot,R.B. and S.C. Battaglene, S.C. (1991). The effect <strong>of</strong> temperature in the extensive rearing <strong>of</strong> Australian Bass,<br />
Macquaria ria Novemaculeata (Steindachner). August 1991, Workshop proceedings ‐ Larval Biology.<br />
The Australian Pesticides and <strong>Veterinary</strong> Medicines Authority (APVMA)’s “Spray Drift Assessment Relevant to<br />
Suspected Adverse Events in a Fish Hatchery Adjacent to a Macadamia Farm – Chemical Review Work Order<br />
CR299‐1, 17 September 2009 Prepared by : Australian Environment Agency.<br />
Umezu, T. (1991). Saponins and Surfactants Increase Water Flux in Fish Gills. Nippon Suisan Gakkaishi (formerly<br />
Bull. Japan. Soc. Sci. Fish.) 57(10), 1891‐1896 (1991).<br />
Van der Ven, L.T.M, Holbech, H., Fenske, M., Van den Brandh<strong>of</strong>, E‐J., Gielis‐Proper, F.K. and Wester, P.W. (2003).<br />
Vitellogenin expression in zebrafish Danio rerio : evaluation by histochemistry, immunohistochemistry, and in situ<br />
mRNA hybridisation. Aquatic Toxicology 65 (2003) 1‐11.<br />
Wedemeyer, G.A. (1996) Physiology <strong>of</strong> Fish in Intensive Culture Systems , Chapmand and Hall.<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
Page 24 <strong>of</strong> 27
Weiss, S.J. (1989). Tissue destruction by neutrophils. N Engl J Med 320:365‐<strong>37</strong>6.<br />
Wilhelm, F.D.(2007). Reactive oxygen species, antioxidants and fish mitochondria. Front Biosci. 2007 Jan<br />
1;12:1229‐<strong>37</strong>.<br />
Woo, P.T.K. 1999 Fish Diseases and Disorders CABI Publishing.<br />
Woo, P.T.K. and Poynton, S.L. (1995). Fish Diseases and Disorders – Volume 1 Protozoan and Metazoan<br />
Infections. Pp. 41‐45.<br />
Yen, C.H., Hsieh, C.Y., Miaw, C.L., Hsieh, C.C., Tseng, H.C. and Yang, Y.H. ∙ (2009), Effects <strong>of</strong> chronic 4‐n‐<br />
nonylphenol treatment on aortic vasoconstriction and vasorelaxation in rats. Arch. Toxicol. (2009) 83:941–946.<br />
Zaroogian, G., Gardner, G., Horowitz Borsay, D., gutjahr‐Gobell, R., Haebler, R. and Mills, L. (2001). Effect <strong>of</strong> 17β‐<br />
estradiol, o,p’‐DDT, octylphenol and p,p’‐DDE on gonadal development and liver and kidney pathology in juvenile<br />
summer flounder (Paralichthys dentatus). Aquatic Toxicology 54 (2001) Issues 1‐2, 101‐112.<br />
Acknowledgements<br />
Acknowledgement for this work is due to :<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
Page 25 <strong>of</strong> 27
o My LORD and Redeemer Jesus Christ whose strength and counsel has guided the field, laboratory and<br />
reporting components <strong>of</strong> this veterinary investigation.<br />
Contributor<br />
And the fish in the Nile died, and the Nile stank, so that the Egyptians could not drink water<br />
from the Nile. There was blood throughout all the land <strong>of</strong> Egypt.<br />
Exodus 7:21<br />
Jesus said to them, “Children, do you have any fish?” They answered him, “No.” He said to<br />
them, “Cast the net on the right side <strong>of</strong> the boat, and you will find some.” So they cast it, and<br />
now they were not able to haul it in, because <strong>of</strong> the quantity <strong>of</strong> fish.<br />
John 21:5<br />
“And wherever the river goes, every living creature that swarms will live, and there will be<br />
very many fish. For this water goes there, that the waters <strong>of</strong> the sea may become fresh; so<br />
everything will live where the river goes.”<br />
Ezekiel 47:9<br />
Position Role<br />
Max Wingfield Fisheries Biologist and Extension Sampling and hatchery inspection<br />
Stephen Were Principal Chemist Chemical residue testing and results<br />
assessment<br />
Patrick Seydel Residue Analyst Chemical residue testing and results<br />
assessment<br />
Alan McMannus Biochemistry – laboratory technician AChE analyses and results assessment<br />
Mo Amigh<br />
Howard Prior<br />
Histotechnologist – senior technician<br />
Histotechnologist<br />
Histology processing<br />
Special stains<br />
Paul Duffy Bacteriology – senior technician Bacterial culture and isolation<br />
Annette Thomas Principal microbiologist Bacteriology and mycology<br />
Dr. Ian Anderson Principal Fish Pathologist Peer review – fish histopathology<br />
Dr. Rachel Bowater Senior <strong>Veterinary</strong> Officer – Fish Diseases Peer review – fish histopathology<br />
Richard Watts Chemical Use and Food Safety – A/Principal<br />
Scientific Advisor<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
Page 26 <strong>of</strong> 27<br />
Pesticides risk assessment<br />
Peer review – AChE<br />
Peer review – 1 st Vet <strong>Report</strong><br />
Preparation <strong>of</strong> Spray Log Transcripts<br />
Ujang Tinggi QHFSS – Senior Scientist Trace Elements Metals residues analysis – fish<br />
Xiaohong Yang QHFSS ‐ Chemist Metals residues analysis – water<br />
David Waltisbuhl Manager – Biosecurity Sciences Laboratory Staff support and laboratory funding<br />
Belinda Fraser Laboratory Technician – Necropsy and<br />
Specimen Receipt<br />
Dr. Ian Douglas<br />
Dr. Munro Mortimer<br />
(Chair <strong>of</strong> SCC), Manager, Animal and Chemical<br />
Biosecurity Sciences, Biosecurity Queensland,<br />
Queensland <strong>Primary</strong> <strong>Industries</strong> & Fisheries,<br />
<strong>Department</strong> <strong>of</strong> Employment, Economic<br />
Development and Innovation.<br />
Senior Principal Scientist ‐ Investigations ,<br />
Fish sampling and necropsy kits<br />
Specimen receipt<br />
Peer review – 1 st Vet <strong>Report</strong><br />
Peer review – 1 st Vet <strong>Report</strong>
Troy Ziesemer<br />
Roger Arbuckle<br />
Gwen Gilson<br />
Bernie<br />
Freshwater and Marine Sciences Unit ,<br />
Division <strong>of</strong> Environmental Sciences ,<br />
Queensland <strong>Department</strong> <strong>of</strong> Environment and<br />
Resource Management.<br />
Macadamia Producers<br />
Sunland Fish Hatchery Australian Native Fish<br />
Producers<br />
<strong>Report</strong> to Noosa Fish Health Investigation Task Force<br />
<strong>7th</strong> <strong>Veterinary</strong> <strong>Report</strong><br />
Executive Summary <strong>of</strong> <strong>Veterinary</strong> Investigations<br />
Page 27 <strong>of</strong> 27<br />
Spray Log Information<br />
Hatchery records<br />
APVMA<br />
Australian Pesticides and <strong>Veterinary</strong><br />
Medicines Authority<br />
AgDRIFT Spray Modelling <strong>Report</strong><br />
Pat Pepper Senior Principal Scientist (Biometry) Animal<br />
Science<br />
Statistical analysis <strong>of</strong> fish data<br />
Dr. Renee<br />
<strong>Veterinary</strong> Officer Collection <strong>of</strong> soil samples from Sunland<br />
Thompson<br />
Hatchery to Biosecurity Sciences<br />
Laboratory. Investigation <strong>of</strong> horse,<br />
chicken and duck health problems on<br />
Sunland Hatchery.<br />
Dr. Mary Hodge<br />
Simon Christen<br />
Scott Turner<br />
Stewart Carswell<br />
Peter White<br />
Queensland Health and Forensic Services<br />
Residue analytical services.<br />
Ron Glanville Chief <strong>Veterinary</strong> Officer Prioritisation and authorisation <strong>of</strong> the<br />
veterinary investigation.<br />
Jim Thompson Director Biosecurity Science Prioritisation and authorisation <strong>of</strong> the<br />
veterinary investigation.<br />
Ian Douglas Manager Animal Biosecurity Laboratories Prioritisation and authorisation <strong>of</strong> the<br />
veterinary investigation.<br />
Vijay Mareedy (Fisheries Research Biologist), Bribie Island<br />
Research Centre<br />
Eliza Smith (Zoologist, veterinary student) , University <strong>of</strong><br />
Queensland<br />
Russel Scholl Principal Policy Officer, Plant Biosecurity and<br />
Product Integrity<br />
Technical support and assistance in the<br />
egg deformity counts and hatchery<br />
observations<br />
Technical assistance on the hatchery.<br />
For provision <strong>of</strong> macadamia spray log<br />
information on methoxyfenozide