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Flavobacterium psychrophilum, cause of ... - cop.eXtension.org

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et al. 1997), but its morphology may be affected by conditions <strong>of</strong> culture (Kondo et al.<br />

2001).<br />

Figure 1. Micros<strong>cop</strong>ic photo showing <strong>Flavobacterium</strong><br />

<strong>psychrophilum</strong> in a wet mount at 1000X. Photograph by R.<br />

A. Holt<br />

The bacterium is refractile, does not form microcysts or fruiting bodies, but produces<br />

bright yellow colonies not greater than 3 mm in diameter with thin spreading margins<br />

(Figure 2) on specialized agar (B<strong>org</strong> 1960; Pacha 1968). Additional phenotypic<br />

characteristics are presented in Table 2.<br />

Figure 2. <strong>Flavobacterium</strong> <strong>psychrophilum</strong><br />

colonies with thin spreading periphery on<br />

Cytophaga Agar. Photograph by R. A. Holt.<br />

Epizootics <strong>of</strong> BCWD commonly occur when water temperatures range between 4 -10 o C<br />

(B<strong>org</strong> 1960), but mortality generally abates as temperatures approach 15 - 18 o C (Rucker<br />

et al. 1953; Holt et al. 1989; Santos et al. 1992). In recent years at some fish hatcheries in<br />

the Pacific Northwest, this disease has been observed to persist and <strong>cause</strong> death at water

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