chromatographic determination of glycoalkaloids in eggplant

3.1.2. Derivatization and SPME ............................................................ 47
3.2. Experimental...................................................................................... 47
3.2.1. Chemicals and Materials.............................................................. 47
3.2.2. Derivatization for GC-MS without SPME................................... 48
3.2.3. Direct SPME with on-fiber Derivatization .................................. 48
3.2.4. GC-MS Analysis........................................................................... 49
3.3. Results and Discussion ....................................................................... 50
CHAPTER 4. CONCLUSION..................................................................................... 58
REFERENCES .............................................................................................................. 60
APPENDICES
APPENDIX A EFFECT OF BUFFER CONCENTRATION AND ADDITION
OF METHANOL TO THE MOBILE PHASE..................................... 68
APPENDIX B OVERALL EFFECT OF BUFFER TYPE ON SEPARATION OF
GLYCOALKALOIDS.......................................................................... 70
APPENDIX C EFFECT OF TEMPERATURE............................................................ 73
APPENDIX D EFFECT OF TYPE OF MOBILE PHASE .......................................... 74
APPENDIX E GRADIENT METHOD........................................................................ 76
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