v - MSpace at the University of Manitoba

Scientific, Edmonton, Alberta, Canada). Following drying under a fbmahood, the meals wen re-
ground to pas through a 1 mm sieve and were re-extracted with n-hexane for m additional 8 hours.
The dried meals were moist heat treated at 108 *lac for 20 minutes in a Iaboratory cyclomatic
thermal sterilizer (autoclave) and then dried overnight at 40°C. AU of our meah sbowed the
myrosinase activity below 0.05 unitfg (see section below for unit definition). For chexnical analyses,
the me& were re-ground to pass through a 1 mm sieve using a Tecator Cyclotec 1093 sample mill
(Fisher Scientific).
2.2.2. Chernical Analysis
The meal samples were analyzed in duplicate for dry matter, crude protein (Kjeldahl N x 6.25),
ether extract, ash and phosphorus using established methods of analysis (AOAC, 1990). Phytate
phosphorus was determined by the method of Hang and Lantzsch (1983). Non-phytate phosphorus
was calculated by difference between total and phytate phosphorus. Dietary fiber was determined
by a combination of neutral detergent fiber (NDF) and neutral detergent-soluble nonstarch
polysaccharide measurements and was calculated as the sum of NDF and detergent soluble NSP
(Slominski et al., 1994a). The method of Goering and Van Soest (1970) was used for the
determination of NDF, except that the procedure was modified to exclude the use of decalin and
sodium sulfite (Mascharanhas Ferreira et al. 1983). Non-starch polysaccharides (NSP) were
measured by gas-liquid chromatography (component neutral sugars) and by colorimetry (uronic acids)
using the procedure descnbed by Englyst and Cummings (1984, 1988) with minor modifications
(Slominski and Campbell, 1990). The NSP content was determined in both the meals and the NDF
residues. Neutra1 detergent-soluble NSP was calculated as total sarnple NSP minus NSP present in
the NDF residue. Sucrose and galactooligosaccharides (dnose and stachyose) were determineci
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