Chlamydia trachomatis - UK NEQAS for MICROBIOLOGY
Chlamydia trachomatis - UK NEQAS for MICROBIOLOGY
Chlamydia trachomatis - UK NEQAS for MICROBIOLOGY
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<strong>UK</strong> National External Quality Assessment Service <strong>for</strong> Microbiology<br />
<strong>UK</strong> <strong>NEQAS</strong> <strong>for</strong> Microbiology<br />
PO Box 63003<br />
London NW9 1GH<br />
Phone +44 (0)20 8905 9890 Fax +44 (0)20 8205 1488<br />
Hosted by the Health Protection Agency - Colindale<br />
Distribution : 2769<br />
Dispatch Date : 28-Feb-2011<br />
Intended Result Your Report<br />
<strong>for</strong> <strong>Chlamydia</strong> detect. (molecular)<br />
Laboratory :<br />
Page 1 of 3<br />
Specimen 0203 <strong>Chlamydia</strong> <strong>trachomatis</strong> DNA positive <strong>Chlamydia</strong> <strong>trachomatis</strong> DNA positive 2<br />
Specimen 0204 <strong>Chlamydia</strong> <strong>trachomatis</strong> DNA positive <strong>Chlamydia</strong> <strong>trachomatis</strong> DNA positive 2<br />
Specimen 0205 <strong>Chlamydia</strong> <strong>trachomatis</strong> DNA negative <strong>Chlamydia</strong> <strong>trachomatis</strong> DNA negative 2<br />
Specimen 0206 <strong>Chlamydia</strong> <strong>trachomatis</strong> DNA positive <strong>Chlamydia</strong> <strong>trachomatis</strong> DNA positive 2<br />
Cumulative score in<strong>for</strong>mation<br />
Total number of specimens sent to you <strong>for</strong> <strong>UK</strong> <strong>NEQAS</strong> <strong>for</strong> <strong>Chlamydia</strong> detect. (molecular) over the last 3 distributions is 12.<br />
For these distributions specimen numbers 9867 9868 9869 9870 0080 0081 0082 0083 0203 0204 0205 0206 have been analysed and scored.<br />
Number of reports analysed 12<br />
Number of specimens received too late <strong>for</strong> analysis (not scored) 0<br />
Number of specimens <strong>for</strong> which no report was received (scored as 0) 0<br />
Your cumulative score <strong>for</strong> these specimens was 24 out of a possible total of 24<br />
Your Score<br />
The mean score calculated from the reports returned by <strong>UK</strong> laboratories was 23.12 (with a standard error of 1.94)<br />
Your per<strong>for</strong>mance rating <strong>for</strong> <strong>UK</strong> <strong>NEQAS</strong> <strong>for</strong> <strong>Chlamydia</strong> detect. (molecular) (i.e. the number of standard errors by which your cumulative score lies above or below<br />
the mean) <strong>for</strong> <strong>UK</strong> laboratories is 0.45.<br />
A per<strong>for</strong>mance rating of more than 1.96 standard errors below the mean indicates possible poor per<strong>for</strong>mance.<br />
Please note your per<strong>for</strong>mance rating may alter if other participants' results are amended.<br />
Total score<br />
4<br />
3<br />
2<br />
1<br />
0<br />
-1<br />
-2<br />
-3<br />
-4<br />
Your per<strong>for</strong>mance rating over the past 12 distributions<br />
Your current per<strong>for</strong>mance rating is 0.45<br />
2176 2216 2264 2319 2359 2402 2472 2521 2586 2667 2731 2769<br />
Distribution<br />
The graphs presented above provide a graphical representation of per<strong>for</strong>mance over the past 12 distributions.<br />
Turn around time, web users only: The time taken to report your results was 0 days (a value is only available <strong>for</strong> web users). This in<strong>for</strong>mation is provided <strong>for</strong> your<br />
own use and does not <strong>for</strong>m part of your per<strong>for</strong>mance assessment.<br />
Comments:<br />
A total of 279 sets of specimens were distributed <strong>for</strong> testing with 270 participants returning results within the specified period: one of these participants did not<br />
examine the specimens.<br />
The overall per<strong>for</strong>mance <strong>for</strong> all scored specimens was very good with 98.6% of participants reporting the correct result. Pre-distribution testing confirmed the<br />
presence of both genomic and cryptic plasmid DNA <strong>for</strong> all positive specimens.<br />
Specimens 0203, 0204 and 0206 all originated from a single cultured C. <strong>trachomatis</strong> (serovar K) clinical sample, with an original estimated quantification of 2.3-7.5 x<br />
10 4 EB/mL, and diluted 1:143, 1:30 and 1:50 respectively.<br />
Specimen 0205 was a negative specimen and consisted of the freeze-drying matrix only. Six participants reported a false positive results and two participants gave<br />
an overall equivocal report.<br />
A breakdown of methods used is given in the histograms, and includes repeat testing results where the second method was different from the initial method.<br />
April 2011: We are introducing web only reporting from April 2011. For this distribution 82% of participants returned results using the web reply <strong>for</strong>ms.<br />
Acknowledgements: We would like to thank colleagues from HPA Bristol, HPA Cambridge, University Hospital Aintree, Liverpool, and St Mary’s Hospital, London,<br />
<strong>for</strong> their assistance with pre-distribution testing.<br />
Enquiries: Written enquiries about this distribution should be addressed to Dr Brigitte Senechal at the e-mail address below.<br />
For repeat specimens please fax us on +44 (0)20 8205 1488 or e-mail:<br />
organiser@ukneqasmicro.org.uk<br />
stating your laboratory identification number, the distribution name and number and specimen number(s).<br />
All<br />
<strong>UK</strong><br />
Total score<br />
18<br />
15<br />
12<br />
9<br />
6<br />
3<br />
0<br />
Total score you achieved <strong>for</strong> each of the last 12 distributions<br />
Your current total score is 8<br />
2176 2216 2264 2319 2359 2402 2472 2521 2586 2667 2731 2769<br />
Distribution<br />
© Copyright. The data in <strong>UK</strong> <strong>NEQAS</strong> reports are confidential.<br />
Participants must consult the scheme organiser be<strong>for</strong>e quoting data from the<br />
scheme.<br />
Published at 11:59:40 on Wednesday 13 July 2011
<strong>UK</strong> National External Quality Assessment Service <strong>for</strong> Microbiology<br />
Distribution : 2769<br />
Dispatch Date : 28-Feb-2011<br />
<strong>for</strong> <strong>Chlamydia</strong> detect. (molecular)<br />
Laboratory :<br />
Page 2 of 3<br />
Specimens 0203, 0204 and 0206 originated from a single cultured C. <strong>trachomatis</strong> clinical sample (serovar K) diluted 1:143, 1:30 and 1:50 respectively. Specimen<br />
0205 was a negative specimen and consisted of the freeze-drying matrix only.<br />
Specimen : 0203 <strong>Chlamydia</strong> <strong>trachomatis</strong> DNA positive<br />
Qiagen: Artus<br />
Real-Time Single target<br />
GeneProof: Real-Time<br />
Qiagen: ArtusPLUS<br />
Real-Time Multiplex<br />
Nuclear Laser Medicine<br />
Gen-Probe : PACE Mono<br />
TIB MOLBIOL: LightMix<br />
Sacace Real-TM<br />
PCR: Single target<br />
Unspecified<br />
Roche: Amplicor<br />
Seegene Seeplex STD<br />
Roche : Cobas TaqMan v2<br />
Roche: Cobas Amplicor<br />
Nanogen AD: Real-Time Alert<br />
Gen-Probe : APTIMA Mono<br />
Gen-Probe : APTIMA Combo<br />
Roche: Cobas 4800<br />
BD: Qx Amplified (Viper)<br />
Abbott RealTime<br />
Roche : Cobas TaqMan<br />
BD : ProbeTec<br />
0 16 32 48 64 80<br />
Number of Reports<br />
Specimen : 0204 <strong>Chlamydia</strong> <strong>trachomatis</strong> DNA positive<br />
Qiagen: Artus<br />
Real-Time Single target<br />
GeneProof: Real-Time<br />
Qiagen: ArtusPLUS<br />
Real-Time Multiplex<br />
Nuclear Laser Medicine<br />
Gen-Probe : PACE Mono<br />
TIB MOLBIOL: LightMix<br />
Sacace Real-TM<br />
PCR: Single target<br />
Unspecified<br />
Roche: Amplicor<br />
Seegene Seeplex STD<br />
Roche : Cobas TaqMan v2<br />
Roche: Cobas Amplicor<br />
Nanogen AD: Real-Time Alert<br />
Gen-Probe : APTIMA Mono<br />
Gen-Probe : APTIMA Combo<br />
Roche: Cobas 4800<br />
BD: Qx Amplified (Viper)<br />
Abbott RealTime<br />
Roche : Cobas TaqMan<br />
BD : ProbeTec<br />
<strong>UK</strong> <strong>NEQAS</strong> <strong>for</strong> Microbiology<br />
PO Box 63003<br />
London NW9 1GH<br />
Phone +44 (0)20 8905 9890 Fax +44 (0)20 8205 1488<br />
Hosted by the Health Protection Agency - Colindale<br />
Positive Equivocal Negative<br />
0 16 32 48 64 80<br />
Number of Reports<br />
Positive Equivocal Negative<br />
<strong>UK</strong> (%) All (%)<br />
2 (1.1) 8 (2.7)<br />
4 (2.3) 10 (3.3)<br />
0 (0.0) 1 (0.3)<br />
1 (0.6) 1 (0.3)<br />
0 (0.0) 1 (0.3)<br />
0 (0.0) 1 (0.3)<br />
0 (0.0) 1 (0.3)<br />
0 (0.0) 2 (0.7)<br />
0 (0.0) 2 (0.7)<br />
0 (0.0) 3 (1.0)<br />
0 (0.0) 3 (1.0)<br />
2 (1.1) 4 (1.3)<br />
0 (0.0) 5 (1.7)<br />
4 (2.3) 7 (2.3)<br />
7 (4.0) 13 (4.3)<br />
2 (1.1) 14 (4.7)<br />
13 (7.4) 15 (5.0)<br />
15 (8.5) 18 (6.0)<br />
17 (9.7) 20 (6.7)<br />
19 (10.8) 25 (8.3)<br />
15 (8.5) 30 (10.0)<br />
24 (13.6) 47 (15.7)<br />
51 (29.0) 69 (23.0)<br />
<strong>UK</strong> (%) All (%)<br />
2 (1.1) 8 (2.7)<br />
4 (2.3) 10 (3.3)<br />
0 (0.0) 1 (0.3)<br />
1 (0.6) 1 (0.3)<br />
0 (0.0) 1 (0.3)<br />
0 (0.0) 1 (0.3)<br />
0 (0.0) 1 (0.3)<br />
0 (0.0) 2 (0.7)<br />
0 (0.0) 2 (0.7)<br />
0 (0.0) 3 (1.0)<br />
0 (0.0) 3 (1.0)<br />
2 (1.1) 4 (1.3)<br />
0 (0.0) 5 (1.7)<br />
4 (2.3) 7 (2.3)<br />
7 (4.0) 13 (4.3)<br />
2 (1.1) 14 (4.7)<br />
13 (7.4) 15 (5.0)<br />
15 (8.5) 18 (6.0)<br />
17 (9.7) 20 (6.7)<br />
19 (10.8) 25 (8.3)<br />
15 (8.5) 30 (10.0)<br />
24 (13.6) 47 (15.7)<br />
51 (29.0) 69 (23.0)<br />
Your Report : <strong>Chlamydia</strong> <strong>trachomatis</strong> DNA<br />
positive<br />
Your Score : 2<br />
Overall Results<br />
<strong>UK</strong> All Score<br />
------------------------------------------------------------<br />
Positive<br />
153 266 2<br />
Equivocal<br />
0 0 1<br />
Negative<br />
0 1 -1<br />
------------------------------------------------------------<br />
Total<br />
153 267<br />
% Correct<br />
100 99.6<br />
Your Report : <strong>Chlamydia</strong> <strong>trachomatis</strong> DNA<br />
positive<br />
Your Score : 2<br />
Overall Results<br />
<strong>UK</strong> All Score<br />
------------------------------------------------------------<br />
Positive<br />
152 265 2<br />
Equivocal<br />
0 1 1<br />
Negative<br />
1 1 -1<br />
------------------------------------------------------------<br />
Total<br />
153 267<br />
% Correct<br />
99.3 99.3<br />
© Copyright. The data in <strong>UK</strong> <strong>NEQAS</strong> reports are confidential.<br />
Participants must consult the scheme organiser be<strong>for</strong>e quoting data from the<br />
scheme.<br />
Published at 11:59:42 on Wednesday 13 July 2011
<strong>UK</strong> National External Quality Assessment Service <strong>for</strong> Microbiology<br />
Distribution : 2769<br />
Dispatch Date : 28-Feb-2011<br />
<strong>for</strong> <strong>Chlamydia</strong> detect. (molecular)<br />
Laboratory :<br />
Page 3 of 3<br />
Specimens 0203, 0204 and 0206 originated from a single cultured C. <strong>trachomatis</strong> clinical sample (serovar K) diluted 1:143, 1:30 and 1:50 respectively. Specimen<br />
0205 was a negative specimen and consisted of the freeze-drying matrix only.<br />
Specimen : 0205 <strong>Chlamydia</strong> <strong>trachomatis</strong> DNA negative<br />
Qiagen: Artus<br />
Real-Time Single target<br />
GeneProof: Real-Time<br />
Qiagen: ArtusPLUS<br />
Real-Time Multiplex<br />
Nuclear Laser Medicine<br />
Gen-Probe : PACE Mono<br />
TIB MOLBIOL: LightMix<br />
Sacace Real-TM<br />
PCR: Single target<br />
Unspecified<br />
Roche: Amplicor<br />
Seegene Seeplex STD<br />
Roche : Cobas TaqMan v2<br />
Gen-Probe : APTIMA Mono<br />
Nanogen AD: Real-Time Alert<br />
Roche: Cobas Amplicor<br />
Gen-Probe : APTIMA Combo<br />
Roche: Cobas 4800<br />
BD: Qx Amplified (Viper)<br />
Abbott RealTime<br />
Roche : Cobas TaqMan<br />
BD : ProbeTec<br />
0 16 32 48 64 80<br />
Number of Reports<br />
Specimen : 0206 <strong>Chlamydia</strong> <strong>trachomatis</strong> DNA positive<br />
Qiagen: Artus<br />
Real-Time Single target<br />
GeneProof: Real-Time<br />
Qiagen: ArtusPLUS<br />
Real-Time Multiplex<br />
Nuclear Laser Medicine<br />
Gen-Probe : PACE Mono<br />
TIB MOLBIOL: LightMix<br />
Sacace Real-TM<br />
PCR: Single target<br />
Unspecified<br />
Roche: Amplicor<br />
Seegene Seeplex STD<br />
Roche : Cobas TaqMan v2<br />
Roche: Cobas Amplicor<br />
Nanogen AD: Real-Time Alert<br />
Gen-Probe : APTIMA Mono<br />
Gen-Probe : APTIMA Combo<br />
Roche: Cobas 4800<br />
BD: Qx Amplified (Viper)<br />
Abbott RealTime<br />
Roche : Cobas TaqMan<br />
BD : ProbeTec<br />
<strong>UK</strong> <strong>NEQAS</strong> <strong>for</strong> Microbiology<br />
PO Box 63003<br />
London NW9 1GH<br />
Phone +44 (0)20 8905 9890 Fax +44 (0)20 8205 1488<br />
Hosted by the Health Protection Agency - Colindale<br />
Positive Equivocal Negative<br />
0 16 32 48 64 80<br />
Number of Reports<br />
Positive Equivocal Negative<br />
<strong>UK</strong> (%) All (%)<br />
2 (1.2) 8 (2.7)<br />
3 (1.8) 9 (3.1)<br />
0 (0.0) 1 (0.3)<br />
1 (0.6) 1 (0.3)<br />
0 (0.0) 1 (0.3)<br />
0 (0.0) 1 (0.3)<br />
0 (0.0) 1 (0.3)<br />
0 (0.0) 2 (0.7)<br />
0 (0.0) 2 (0.7)<br />
0 (0.0) 3 (1.0)<br />
0 (0.0) 3 (1.0)<br />
2 (1.2) 4 (1.4)<br />
0 (0.0) 5 (1.7)<br />
4 (2.4) 7 (2.4)<br />
6 (3.6) 8 (2.7)<br />
1 (0.6) 13 (4.5)<br />
7 (4.2) 13 (4.5)<br />
15 (9.0) 18 (6.2)<br />
17 (10.2) 20 (6.9)<br />
19 (11.4) 25 (8.6)<br />
15 (9.0) 30 (10.3)<br />
24 (14.4) 47 (16.2)<br />
51 (30.5) 69 (23.7)<br />
<strong>UK</strong> (%) All (%)<br />
2 (1.1) 8 (2.7)<br />
4 (2.3) 10 (3.3)<br />
0 (0.0) 1 (0.3)<br />
1 (0.6) 1 (0.3)<br />
0 (0.0) 1 (0.3)<br />
0 (0.0) 1 (0.3)<br />
0 (0.0) 1 (0.3)<br />
0 (0.0) 2 (0.7)<br />
0 (0.0) 2 (0.7)<br />
0 (0.0) 3 (1.0)<br />
0 (0.0) 3 (1.0)<br />
2 (1.1) 4 (1.3)<br />
0 (0.0) 5 (1.7)<br />
4 (2.3) 7 (2.3)<br />
7 (4.0) 13 (4.3)<br />
2 (1.1) 14 (4.7)<br />
13 (7.4) 15 (5.0)<br />
15 (8.5) 18 (6.0)<br />
17 (9.7) 20 (6.7)<br />
19 (10.8) 25 (8.3)<br />
15 (8.5) 30 (10.0)<br />
24 (13.6) 47 (15.7)<br />
51 (29.0) 69 (23.0)<br />
Your Report : <strong>Chlamydia</strong> <strong>trachomatis</strong> DNA<br />
negative<br />
Your Score : 2<br />
Overall Results<br />
<strong>UK</strong> All Score<br />
------------------------------------------------------------<br />
Positive<br />
6 6 -1<br />
Equivocal<br />
0 2 1<br />
Negative<br />
147 259 2<br />
------------------------------------------------------------<br />
Total<br />
153 267<br />
% Correct<br />
96.1 97.0<br />
Your Report : <strong>Chlamydia</strong> <strong>trachomatis</strong> DNA<br />
positive<br />
Your Score : 2<br />
Overall Results<br />
<strong>UK</strong> All Score<br />
------------------------------------------------------------<br />
Positive<br />
149 263 2<br />
Equivocal<br />
0 0 1<br />
Negative<br />
4 4 -1<br />
------------------------------------------------------------<br />
Total<br />
153 267<br />
% Correct<br />
97.4 98.5<br />
© Copyright. The data in <strong>UK</strong> <strong>NEQAS</strong> reports are confidential.<br />
Participants must consult the scheme organiser be<strong>for</strong>e quoting data from the<br />
scheme.<br />
Published at 11:59:42 on Wednesday 13 July 2011
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