Analytical and Quantitative Light Microscopy 2012 Schedule
Analytical and Quantitative Light Microscopy 2012 Schedule
Analytical and Quantitative Light Microscopy 2012 Schedule
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<strong>Analytical</strong> <strong>and</strong> <strong>Quantitative</strong> <strong>Light</strong> <strong>Microscopy</strong><br />
in Biology, Medicine, Materials Science<br />
Wednesday, 5/9/12<br />
2:00 – 3:00 Check in <strong>and</strong> get picture ID<br />
3:00 – 3:30 Opening Address<br />
Marine Biological Laboratory<br />
Woods Hole, MA, USA<br />
<strong>2012</strong> <strong>Schedule</strong><br />
Welcome <strong>and</strong> Introduction of Academic<br />
Faculty<br />
Borisy/Reznikoff<br />
Sluder<br />
Overview of Course / <strong>Quantitative</strong><br />
<strong>Microscopy</strong><br />
Wolf/Swedlow<br />
3:30- 4:15 Geometrical Optics & Image Formation Sluder<br />
4:15 - 5:00 Wave Optics & Image Formation: Resolution Wolf<br />
<strong>and</strong> Abbe's Theory of the Microscope<br />
5:00-6:00 Opening Reception <strong>and</strong> Introduction of<br />
Students (Meigs Room in Swope Center)<br />
Sluder/Wolf/Swedlow<br />
7:00 - 7:45 Wave Optics of Image Formation: Spatial<br />
Frequencies <strong>and</strong> the Fourier Transform<br />
Wolf<br />
7:45-8:30 Practical Aspects of <strong>Microscopy</strong> <strong>and</strong> Kohler<br />
illumination<br />
Sluder<br />
8:30 - 9:15 Demonstration: Abbe Diffraction Apparatus Rottenfusser<br />
9:30 - 11:00 Introduction to equipment stations Commercial Faculty<br />
Thursday, 5/10/12<br />
8:45 - 9:00 Overview Wolf<br />
9:00 - 11:30 Laboratory--Microscope alignment <strong>and</strong> Sluder/Commercial<br />
Kohler illumination<br />
Faculty<br />
11:30-12:00 Point Spread Functions Goodwin<br />
1:00 - 1:45 Practical aspects of lens design Rottenfusser<br />
1:45 - 2:30 Phase Contrast <strong>Microscopy</strong> Sluder<br />
2:30 - 4:00 Phase Contrast Laboratory Sluder/<br />
Commercial Faculty<br />
4:00 - 5:30 Introduction to Atomic, Molecular, <strong>and</strong><br />
Nanostructure Fluorescence<br />
Wolf/Shah<br />
7:00 - 8:00 Fluorescence <strong>Microscopy</strong> Wolf/Shah<br />
8:00 - 12:00 Fluorescence <strong>Microscopy</strong> Laboratory Wolf/Shah/W.Salmon<br />
/Commercial Faculty<br />
AQLM <strong>2012</strong> <strong>Schedule</strong> 1
Friday, 5/11/12<br />
8:45 - 9:00 Overview Swedlow<br />
9:00 – 10:30 Electronic Imaging <strong>and</strong> Digital Image<br />
Processing I<br />
Swedlow<br />
10:30 -12:00 <strong>Quantitative</strong> Laboratory I<br />
Swedlow/Wolf/W.<br />
Salmon<br />
1:00 - 3:00 <strong>Quantitative</strong> Laboratory I, cont Swedlow/Wolf/W.<br />
Salmon<br />
3:00 - 4:00 Quant Laboratory Discussion Wolf/Swedlow<br />
4:00 - 5:30 Cameras for Low <strong>Light</strong> Level Imaging Moomaw<br />
7:00 - 8:00 What’s in a picture? The temptation of image Williams<br />
manipulation<br />
8:00 - 11:30 Low <strong>Light</strong> Level Imaging <strong>and</strong> Signal Swedlow/Wolf/<br />
Quantification Laboratory<br />
Commercial Faculty<br />
Saturday, 5/12/12<br />
8:45 - 9:00 Overview Sluder<br />
9:00 - 10:30 Polarized light microscopy T. Salmon<br />
10:30 12:00 Polarized light laboratory Sluder/Commercial<br />
Faculty<br />
1:00 - 2:30 DIC <strong>and</strong> Enhanced DIC Lecture T. Salmon<br />
2:30 - 5:30 DIC & Enhanced Laboratory<br />
Opportunity to visit <strong>and</strong> use LC PolScope<br />
with R. Oldenbourg<br />
Sluder/Oldenbourg/<br />
Commercial Faculty<br />
7:00 - 8:00 Digital Image Processing II Swedlow<br />
8:00 - 10:30 Image Processing Laboratory Swedlow/Wolf/<br />
10:30 - 11:00 Discussion of imaging processing &<br />
quantitative measurements<br />
Commercial Faculty<br />
Swedlow/Wolf/W.<br />
Salmon<br />
Sunday, 5/13/12<br />
8:45 - 9:00 Overview Wolf<br />
9:00 - 10:30 Fluorescent Probes <strong>and</strong> Proteins Davidson<br />
11:00 - 12:00 Live Cell Imaging Sluder<br />
1:00 - 5:00 Fluorescent Protein <strong>and</strong> Live Cell Imaging Davidson/Sluder/<br />
Laboratory<br />
Commercial Faculty<br />
5:00 - 6:00 Fluorescence Lifetime Imaging Dunsby<br />
7:00 - 10:30 FLIM Laboratory Dunsby/Commercial<br />
Faculty<br />
10:30 - 11:00 Discussion of FLIM Laboratory Dunsby/Commercial<br />
Faculty<br />
AQLM <strong>2012</strong> <strong>Schedule</strong> 2
Monday, 5/14/12<br />
8:45 – 9:00 Overview Sluder<br />
9:00 - 10:00 Scanning, Scanning Confocal, <strong>and</strong><br />
multiphoton <strong>Microscopy</strong> I<br />
Murray<br />
10:00 - 12:00 Confocal <strong>and</strong> Multiphoton <strong>Microscopy</strong> Murray/ Commercial<br />
Exercises I<br />
Faculty<br />
1:00 – 2:00 Confocal <strong>Microscopy</strong> II Murray<br />
2:00 – 5:00 Confocal <strong>Microscopy</strong> Exercises II Murray/<br />
Commercial Faculty<br />
5:00 – 5:30 Confocal <strong>Microscopy</strong> Discussion Murray/ Commercial<br />
Faculty<br />
7:00 – 8:00 <strong>Quantitative</strong> Confocal <strong>Microscopy</strong> Murray<br />
8:00 - 11:00 <strong>Quantitative</strong> Confocal <strong>Microscopy</strong> with thick Murray/Commercial<br />
specimens Laboratory<br />
Faculty<br />
11:00 - 11:30 Discussion of Confocal Lab Murray/Commercial<br />
Faculty<br />
Tuesday, 5/15/12<br />
8:45-9:00 Overview Wolf/Swedlow<br />
9:00 – 10:00 Open Imaging Platforms Eliceiri<br />
10:00-12:00 Open Imaging Platforms Laboratory Eliceiri/Swedlow/<br />
Commercial Faculty<br />
1:00-2:00 Object Identification Jaqaman<br />
2:00 - 5:00 Object Identification Laboratory Jaqaman/<br />
Commercial Faculty<br />
7:00 - 8:00 Digital Deconvolution Swedlow<br />
8:00 – 11:00 Digital Deconvolution Laboratory Swedlow/<br />
Commercial Faculty<br />
Wednesday, 5/16/12<br />
8:45 – 9:00 Overview Sluder<br />
9:00 - 10:00 Object Tracking Jaqaman<br />
10:00 - 12:00 Object Tracking Laboratory Jaqaman/Commercial<br />
Faculty<br />
1:00 – 2:00 <strong>Quantitative</strong> Ratio Imaging Hodgson<br />
2:00 – 4:30 <strong>Quantitative</strong> Ratio Imaging Laboratory Hodgson/<br />
Commercial Faculty<br />
4:30 – 5:30 <strong>Quantitative</strong> Phase <strong>Microscopy</strong> Popescu<br />
7:00 – 8:00 Laser Tweezers <strong>and</strong> <strong>Quantitative</strong> Force Tam<br />
Measurement<br />
8:00 - 11:00 <strong>Quantitative</strong> Phase/Laser Tweezers Labs Tam/Popescu/<br />
Commercial Faculty<br />
11:00 - 11:30 Discussion Popescu/Tam/<br />
Commercial Faculty<br />
AQLM <strong>2012</strong> <strong>Schedule</strong> 3
Thursday, 5/17/12<br />
8:45 - 9:00 Overview Swedlow<br />
9:00 - 10:30 Structured Illumination, TIRF <strong>and</strong> single<br />
molecule detection<br />
Taraska<br />
11:00 -12:00 PALM/STORM Dempsey<br />
1:00 - 5:00 TIRF, PALM/STORM, Structured<br />
Dempsey/Taraska/<br />
Illumination Laboratory<br />
Commercial Faculty<br />
5:30 -6:30 Wine <strong>and</strong> Cheese Social (Meigs Room) Directors<br />
7:00 - 10:00 Course Dinner Directors/Commercial<br />
Faculty<br />
Friday, 5/18/12<br />
8:45 – 9:00 Overview Swedlow<br />
9:30- 11:00 Lasers, Microsurgery in the GFP Era: A Cell<br />
Biologist’s Perspective<br />
Khodjakov<br />
12:00 Lunch <strong>and</strong> departure<br />
AQLM <strong>2012</strong> <strong>Schedule</strong> 4
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