Visible Spectro Photometric Determination of Darifenacin
Visible Spectro Photometric Determination of Darifenacin
Visible Spectro Photometric Determination of Darifenacin
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A B S T R A C T<br />
RGUHS Journal <strong>of</strong> Pharmaceutical Sciences<br />
<strong>Visible</strong> <strong>Spectro</strong> <strong>Photometric</strong> <strong>Determination</strong> <strong>of</strong> <strong>Darifenacin</strong> Hydrobromide in<br />
Bulk and Pharmaceutical dosage forms<br />
Original Research Article<br />
1* 2 1 2<br />
NK Sathish , Pradeep V. Khandelwal , IA Chethan and Ashwinee Kumar Shrestha<br />
1 Dept. <strong>of</strong> Pharmaceutical Chemistry, SAC College <strong>of</strong> Pharmacy, BG Nagara - 571448, Mandya Dt., India<br />
2 Dept. <strong>of</strong> Pharmaceutical Chemistry, Bharathi College <strong>of</strong> Pharmacy, Bharathi Nagara, Mandya Dt., India<br />
A simple and sensitive visible spectro photometric method has been developed for the quantitative estimation <strong>of</strong><br />
<strong>Darifenacin</strong> hydrobromide in bulk drugs and pharmaceutical dosage forms. It involves diazotization <strong>of</strong> <strong>Darifenacin</strong><br />
hydrobromide by sodium nitrite in acidic medium followed by complexation with starch \ KI reagent to yield a bluish -<br />
purple colored complex, which shows maximum absorption at 620 nm against distilled water as blank. The results <strong>of</strong><br />
the analysis were found to be 99.67% and 98.95 % for assay with a % RSD ranging from 0.301-0.422 %. The mean %<br />
recovery for formulation first is 99.36 ± 0.491% and for that <strong>of</strong> second formulation is 99.78 ± 0.235%.<br />
Keywords: <strong>Darifenacin</strong> hydrobromide, <strong>Visible</strong> spectro photometry, Diazotization, Recovery, Assay.<br />
RGUHS Journal <strong>of</strong> Pharmaceutical Sciences<br />
Received: 29/01/2011, Modified: 21/02/2011, Accepted: 28/02/2011<br />
45<br />
Fig 1: <strong>Darifenacin</strong> Hydrobromide<br />
0<br />
NH 2<br />
N<br />
0<br />
RJPS, Apr-Jun, 2011/ Vol 1/ Issue 1
NK Sathish et al <strong>Visible</strong> <strong>Spectro</strong> <strong>Photometric</strong> <strong>Determination</strong> <strong>of</strong> <strong>Darifenacin</strong> Hydrobromide in bulk and Pharmaceutical dosage forms<br />
46<br />
RJPS, Apr-Jun, 2011/ Vol 1/ Issue 1
NK Sathish et al <strong>Visible</strong> <strong>Spectro</strong> <strong>Photometric</strong> <strong>Determination</strong> <strong>of</strong> <strong>Darifenacin</strong> Hydrobromide in bulk and Pharmaceutical dosage forms<br />
Table 1: Optical characteristics and precision data<br />
Table 2: Assay <strong>of</strong> commercial formulations.<br />
Formulation Amount Amount % Assay S.D.* % RSD*<br />
labeled found*<br />
(mg) (mg)/tab<br />
DARILONG 7.5 7.475 99.67 0.421 0.422<br />
7.5 Tablets<br />
DARILONG 15 14.842 98.95 0.298 0.301<br />
15 Tablets<br />
*Average <strong>of</strong> six determinations. S.D. is standard deviation, RSD is relative standard deviation.<br />
47<br />
Abs<br />
0.3<br />
0.2<br />
0.1<br />
0<br />
400 500 600 700 800<br />
Wavelength(nm)<br />
RJPS, Apr-Jun, 2011/ Vol 1/ Issue 1
NK Sathish et al <strong>Visible</strong> <strong>Spectro</strong> <strong>Photometric</strong> <strong>Determination</strong> <strong>of</strong> <strong>Darifenacin</strong> Hydrobromide in bulk and Pharmaceutical dosage forms<br />
48<br />
Address for Correspondence:<br />
Dr. N.K. Sathish, Pr<strong>of</strong>essor, Dept. <strong>of</strong> Pharmaceutical Chemistry, SAC College<br />
<strong>of</strong> Pharmacy, BG Nagara - 571 448, Mandya Dt., Karnataka, India.<br />
E-mail : nksathish76@rediffmail.com<br />
RJPS, Apr-Jun, 2011/ Vol 1/ Issue 1
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