Ilko K. Ilev, Ph.D. - Washington Academy of Sciences
Ilko K. Ilev, Ph.D. - Washington Academy of Sciences
Ilko K. Ilev, Ph.D. - Washington Academy of Sciences
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Diffraction Rayleigh Barrier:<br />
A Fundamental Resolution Limit in Nanobiophotonics<br />
LASER BEAM<br />
LENS<br />
Ultrahigh-Resolution (10000x)<br />
Digital Microscope<br />
Rayleigh Diffraction Resolution Limit<br />
theoretically about one-half<br />
<strong>of</strong> the laser wavelength<br />
d lateral<br />
× λ<br />
=<br />
NA<br />
61 . 0<br />
the highest achievable resolution with objective:<br />
about 180 nm lateral (x-y) resolution<br />
about 500 nm axial (z) resolution<br />
Airy Patterns and the Resolution<br />
Limit
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