[PDF] Data File: rProtein A Sepharose Fast Flow

Flow rate (cm/h)
AU
1000
2.0
800
600
400
5 cm bed height
10 cm bed height
1.5
1.0
200
0.5
0.0
0.5 1.0 1.5 2.0
Pressure (bar)
Fig 4. Pressure/flow rate characteristics of rProtein A Sepharose Fast Flow.
The pressure/flow velocity data were determined in a BPG 200/500 column
(200 mm i.d.) packed to a bed height of 5 cm and 10 cm using water as the
mobile phase at 20 °C.
0 200 400 600 Volume (ml)
Fig 5. Purification of a monoclonal IgG2a from clarified cell culture on
rProtein A Sepharose Fast Flow.
Table 5. Recommended columns for rProtein A Sepharose Fast Flow.
Columns Inner Bed volume Bed height
diameter (mm)
(cm)
Lab scale
XK 16/40 16 8–74 ml max. 35
XK 26/40 26 32–196 ml max. 35
1 2 3 4
Fig 6. SDS-PAGE of starting material (lane 2) and
eluate (lane 3). The samples were concentrated
10 times and reduced. Lane 1 and 4 are LMW
markers. PhastSystem, PhastGel Gradient
10–15.
Production scale
BPG variable 100–450 2.4–131 liters max. 83
bed, glass column
BioProcess 400–1400 12–1500 liters 10–100
Stainless Steel
(BPSS) fixed bed
columns
INdEX variable 70–200 Up to 24.8 liters max. 79
bed columns
Chromaflow 280–2000
variable bed columns
Ordering information
Product Pack size Code No.
rProtein A Sepharose Fast Flow 5 ml 17-1279-01
25 ml 17-1279-02
200 ml 17-1279-03
1 L 17-1279-04
5 L 17-1279-05
All bulk media products are supplied in suspension in 20%
ethanol.
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BPG, BioProcess, Chromaflow, HiTrap, INdEX, PhastSystem, PhastGel and
Sepharose are trademarks of General Electric companies. GE, imagination
at work, and GE Monogram are trademarks of General Electric Company.
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© 2008 General Electric Company – All rights reserved.
First published 2002.
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required, to make changes in specifi cations and features shown herein, or
discontinue the product described at any time without notice or obligation.
Contact your local GE Healthcare representative for the most current
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18-1113-94 AE 06/2008
Elanders i Uppsala 2008