External quality assurance scheme for Haemophilus influenzae 2011

More documents

Recommendations

Info

TECHNICAL REPORT External quality assurance scheme for Haemophilus influenzae Contents Abbreviations ................................................................................................................................................ v Executive summary ........................................................................................................................................ 1 Introduction .................................................................................................................................................. 2 1 Material and methods .................................................................................................................................. 3 1.1 Study design ....................................................................................................................................... 3 1.2 Participants......................................................................................................................................... 3 1.3 Timelines ............................................................................................................................................ 3 1.4 The EQA panel material ....................................................................................................................... 4 1.4.1 Bacterial isolates .......................................................................................................................... 4 1.4.2 Non-culture simulated meningitis samples ...................................................................................... 4 2 Results ....................................................................................................................................................... 5 2.1 Part 1: Characterisation of viable isolates .............................................................................................. 6 2.1.1 Phenotypic species identification .................................................................................................. 10 2.1.2 Phenotypic serotyping ................................................................................................................. 10 2.1.3 Biotyping ................................................................................................................................... 10 2.1.4 Genotypic species identification ................................................................................................... 11 2.1.5 Genotypic capsule typing ............................................................................................................ 12 2.1.6 Other molecular typing ............................................................................................................... 12 2.2 Part 2: Antimicrobial susceptibility testing ............................................................................................ 12 2.2.1 β-lactamase activity testing ......................................................................................................... 12 2.2.2 Antimicrobial susceptibility testing ................................................................................................ 12 2.3 Part 3: Non-culture detection of H. influenzae ...................................................................................... 14 2.4 Part 4: Summary comparison of IBD-Labnet H. influenzae EQA panels 2009 and 2011 ............................ 15 Overall comments ........................................................................................................................................ 16 Conclusions ................................................................................................................................................. 18 References .................................................................................................................................................. 19 Annex 1. Participating reference laboratories .................................................................................................. 21 Annex 2. Consensus results for Haemophilus influenzae identification, typing and antimicrobial susceptibility testing 23 Annex 3. Example of report generated by UK NEQAS ...................................................................................... 25 iii
External quality assurance scheme for Haemophilus influenzae TECHNICAL REPORT Figures Figure 1. Strain identification .......................................................................................................................... 8 Figure 2. Phenotypic serotyping ...................................................................................................................... 8 Figure 3. Biotype identification ........................................................................................................................ 9 Figure 4. Genotypic species identification ......................................................................................................... 9 Figure 5. Genotypic capsular typing ................................................................................................................. 9 Tables Table 1. Tests requested from the participating laboratories .............................................................................. 3 Table 2. Timelines for the EQA exercise ........................................................................................................... 3 Table 3. Summary of tests for which each laboratory submitted results a ............................................................. 5 Table 4. Intended results for Part 1: Characterisation of viable isolates ............................................................... 6 Table 5 Results for Part 1: Characterisation of viable isolates ............................................................................. 6 Table 6. Phenotypic species identification methods reported by participating laboratories ................................... 10 Table 7. Summary of biotyping methods used by 20 participating laboratories ................................................... 11 Table 8. Biotyping scheme for Haemophilus influenzae and Haemophilus parainfluenzae (Kilian 1976, Oberhofer and Back 1979, Gratten 1983, Sottnek and Albritton 1984) .............................................................................. 11 a) Biotypes of Haemophilus influenzae ................................................................................................. 11 b) Biotypes of Haemophilus parainfluenzae .......................................................................................... 11 Table 9. Number or participants using various combinations of DNA extraction procedure and detection method for genotypic species identification and capsular typing on viable isolates .............................................................. 12 Table 10. Multilocus sequence types (ST) of samples 0262 to 0267 .................................................................. 12 Table 11. Intended results for antimicrobial susceptibility testing of bacterial isolates ......................................... 13 Table 12. Intended and submitted results for Part 3: Non-culture detection of H. influenzae ............................... 14 Table 13. Methods used for preparation and detection of H. influenzae DNA in simulated CSF samples ................ 14 iv
Page 1 and 2: TECHNICAL REPORT External quality a
Page 3: This report was commissioned by the
Page 29 and 30: Genotypic Identification Phenotypic
Page 55 and 56:
TECHNICAL REPORT External quality a
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
show all

External quality assurance scheme for Haemophilus influenzae 2011

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?