Resource Manual - International Embryo Transfer Society

More documents

Recommendations

Info

IETS CANDES Research Subcommittee E-Mail Addresses of Resource Manual Taxon Leaders Taxon Taxon Leader E-Mail Address Carnivores Jason Herrick jherrick@illinois.edu Ungulates Rebecca L. Krisher rkrisher@fertilityresearch.org Birds Juan Manual Blanco uhofleh@wanadoo.es Amphibians/Reptiles Andrew Kouba akouba@memphiszoo.org Non-Human Primates Justine K. O‘Brien justineo@vetsci.usyd.edu.au Invertebrates Anita Collins collinsa@ba.ars.usda.gov Marsupials Monique C.J. Paris m.paris@uu.nl Fish Boris Dzyuba bdzuba@yahoo.com Marine Mammals Todd Robeck todd.robeck@seaworld.com Rodents Marina F. Ponzio mponzio@mater.fcm.unc.edu.ar Other Taxa Suggestions Requested The IETS CANDES Research Subcommittee Resource Manual includes standard operating procedures (defined here as protocols) referring to all embryo transfer and related technologies applicable to companion animals, non-domestic and endangered species. New information and updates will be posted in March and September annually. Authors may send copies of their protocols for posting on the IETS CANDES web page to the relevant Taxon Leader above. Each new submission must contain contact details (preferably an e-mail address) for queries concerning the protocols and they must include citations of supporting manuscripts and/or data demonstrating application of the procedures. Protocols are specifically requested for the following topics: 1. Gamete/Embryo Collection 2. Embryo Transfer 3. Artificial Insemination 4. In Vitro Fertilization/Culture 5. Cryopreservation 6. Endocrinology Taxon Leaders are needed at this time for ―Birds‖ and ―Marine Mammals‖ and requests are being accepted for other taxa to be included in the Resource Manual. Queries and requests should be sent to the IETS CANDES Research Subcommittee Co-Chairmen: Rebecca L. Krisher (E-Mail: rkrisher@purdue.edu) or Monique C. J. Paris (E-Mail: m_wolvekamp@hotmail.com). Taxon Leaders should have some background knowledge or experience in embryo transfer and related technologies for their specific taxon, and are responsible for recruiting participation from colleagues to submit relevant protocols. 4
Large Felid Semen Collection by Rectal Probe Electrostimulation (Electroejaculation) N.M. Loskutoff Center for Conservation & Research, Omaha‘s Henry Doorly Zoo September 2003 Contact information for further details: Naida Loskutoff at: NaidaL@omahazoo.com Publications using this protocol: 1) Crichton, E.G., E. Bedows, A.K. Miller-Lindhom, D.M. Baldwin, D.L. Armstrong, L.H. Graham, J. Ford, J.O. Gjorret, P. Hyttel, C.E. Pope, G. Vajta and N.M. Loskutoff (2003) The efficacy of porcine gonadotropins for repeated stimulation of ovarian activity for oocyte retrieval and in vitro embryo production and cryopreservation in Siberian tigers (Panthera tigris altaica). Biology of Reproduction 68: 105-113. 2) Gjorret, J.O., Crichton, E.G., Loskutoff, N.M., Armstrong, D.L. and Hyttel, P. Oocyte maturation, fertilization and early embryonic development in vitro in the Siberian tiger (Panthera tigris altaica). Molecular Reproduction and Development 63:79-88, 2002. 3) Nelson, K.L., E.G. Crichton, L. Doty, D. Volenec, J.M. Finnegan, R.G. Morato, C.E. Pope, B.L. Dresser, D.L. Armstrong and N.M. Loskutoff (1999) Heterologous and homologous fertilizing capacity of cryopreserved felid sperm: a model for endangered species. Theriogenology 51:290, abst. 4) Donoghue, A., A. Byers, L. Johnston, D. Armstrong, and D. Wildt. Timing of ovulation after gonadotrophin induction and its importance to successful intrauterine insemination in the tiger (Panthera tigris). Journal of Reproduction and Fertility, 107: pp 53-58. 1996 Materials Needed: Rectal probe (see Fig. 1), sand paper Electrostimulator (e.g., Platz or Beltz models – for contact details, see Technology Subcommittee Resources section) Electrical extension cord Lubricant (non-spermicidal) Latex gloves Paper towels 15 ml conical tubes (sterile) Insulated container Medium (e.g., TL Hepes, Bio Whittaker) Permanent ink pen Warming packs (if necessary) Disinfectant 5
Page 1 and 2: Research Subcommittee Resource Manu
Page 3: VI. Marsupials (Taxon Leader: Moniq
Page 7 and 8: eturn to 0. Once initiated, this pr
Page 9 and 10: In Vitro Fertilization and Embryo T
Page 11 and 12: 3. After overnight soaking in the d
Page 13 and 14: 6. To prepare, 15 mM NaHCO3, 15 mM
Page 15 and 16: 9. After checking for patency the b
Page 17 and 18: 2. Three abdominal entry sites are
Page 19 and 20: 6 Poliglecaprone 25 is the preferre
Page 21 and 22: IVF 1. The IVF dishes are prepared
Page 23 and 24: Notes 1. Each new BSA lot is tested
Page 25 and 26: 11. Pope C. E., McRae, M. A., Plair
Page 27 and 28: Cryodiluents: generally, a TES-Tris
Page 29 and 30: 5. To isolate the live and motile s
Page 31 and 32: ( Andersen, 1975, Farstad, 1984, Fa
Page 33 and 34: ELISA Protocol: Measuring Anti-Gona
Page 35 and 36: Assay Notes: Blocking - If desired,
Page 37 and 38: ~ 40 cm length ~ 5 cm ~ 6 cm ~ 4 cm
Page 39 and 40: Worksheet: Semen Collection by Rect
Page 41 and 42: 6000iu. It is generally injected in
Page 43 and 44: Figure 1. Transcervical embryo coll
Page 45 and 46: Criteria for selection of recipient
Page 47 and 48: Contact Information for further det
Page 49 and 50: 7. Clean up the microscope and aspi
Page 51 and 52: In Vitro Culture 1. The presumptive
Page 53 and 54: STOCK EDTA (edta) 100X 1 month expi
Page 55 and 56:
Wild & Domestic Cattle, Buffalo and
Page 57 and 58:
8. To thaw, individual straws shoul
Page 59 and 60:
Cryodiluents: generally, a Tris-cit
Page 61 and 62:
(semen:water) is made by adding 10
Page 63 and 64:
A total of 200 sperm should be coun
Page 65 and 66:
Epididymal Sperm Collection and Pro
Page 67 and 68:
more aviculturists to attempt AI wh
Page 69 and 70:
emaining semen is sufficient for mi
Page 71 and 72:
Semen Collection in Snakes Submitte
Page 73 and 74:
Artificial Insemination of Snakes S
Page 75 and 76:
1996). Oocytes with >10 sperm penet
Page 77 and 78:
Gorilla Sperm Cryopreservation Prot
Page 79 and 80:
Gorilla Sperm Cryopreservation Prot
Page 81 and 82:
Great Ape Semen Cryopreservation: E
Page 83 and 84:
4. After a minimum of two hours in
Page 85 and 86:
8. Plunge the pellets directly into
Page 87 and 88:
Semen/Spermatozoa Collection and Ev
Page 89 and 90:
Epididymal flushing If flush is c
Page 91 and 92:
Component B = 5 ml of 2.4 mM propid
Page 93 and 94:
Superovulation of Female Brushtail
Page 95 and 96:
Superovulation in Wombat Species (e
Page 97 and 98:
Once prepared, the solutions can be
Page 99 and 100:
day 6 mature oocytes are collected
Page 101 and 102:
Table.1 Drugs administered to super
Page 103 and 104:
Biosciences NZ Ltd.), supplemented
Page 105 and 106:
Light microscopy - acetic lacmoid s
Page 107 and 108:
Gauze Elastic band (Garrot) to visu
Page 109 and 110:
Wombat Faecal Progesterone Metaboli
Page 111 and 112:
Preparation of dilutions for the MT
Page 113 and 114:
Stop reaction (should turn yellow)
Page 115 and 116:
10. Count 2-5ul from each fraction
Page 117 and 118:
Contact Information for further det
Page 119 and 120:
Summary: Media and Products a. For
Page 121 and 122:
Steps in preparing specimens for cr
Page 123 and 124:
Hybrimax, D-2650)) and 0.1 M sucros
Page 125 and 126:
Acknowledgements: I thank Monash IV
Page 127 and 128:
acrosome presence in barbel sturgeo
Page 129 and 130:
Next step is express assessment of
Page 131 and 132:
Measurement Techniques for Fish Lar
Page 133 and 134:
Chinchilla lanigera Order: Rodentia
Page 135 and 136:
1000 x magnification in an epifluor
Page 137 and 138:
Cortisol Enzyme Immunoassay Protoco
show all

Resource Manual - International Embryo Transfer Society

Create successful ePaper yourself

Delete template?

Save as template?