C.I. Pigment Brown 24 CAS N°: 68186-90-3

OECD SIDS C.I. PIGMENT BROWN 24
ECOTOXICITY ID 68186-90-3
DATE: 26-NOV-2004
Exposure period: 72 hour(s)
Unit: mg/l Analytical monitoring: no
NOEC: >= 100
LOEC: > 100
EC10: > 100
EC50: > 100
EC90 : > 100
Method:
OECD Guide-line 201 "Algae, Growth Inhibition Test"
Year: 1984
GLP:
yes
Test substance: as prescribed by 1.1 - 1.4
Method: Test also according to EEC directive 92/69/EEC, Annex V,
Part C.3. (1992) and EPA OPPTS 850.5400 (1996)
Remark:
MONITORING OF TEST SUBSTANCE CONCENTRATION:
Test performed with an aqueous extract (filtrate) of the TS.
Test performed without concentration control analysis
because the recovery rate in a filtrate is below the
detection limit (see also data in section 4.2).
Result:
CONTROL
In negative control cell multiplication factor after 72h:
149-fold; positive control with potassium dichromate EC50
(72h)= 0.41 mg/l;
INHIBITION OF ALGAL BIOMASS AFTER 72h
Concentration in mg TS/l
0 1.56 3.13 6.25 12.5 25 50 100
Inhibition
in % of control 0 -1.6 -4.5 -1.4 0.5 -1.0 1.0 -15.4
EbC10 (72h) > 100 mg/l
EbC50 (72h) > 100 mg/l
EbC90 (72h) > 100 mg/l
INHIBITION OF GROWTH RATES AFTER 72h
Concentration in mg TS/l
0 1.56 3.13 6.25 12.5 25 50 100
Inhibition
in % of control 0 -0.7 -2.0 0.1 -0.3 -2.3 -1.0
-4.0
Test condition:
ErC10 (72h) > 100 mg/l
ErC50 (72h) > 100 mg/l
ErC90 (72h) > 100 mg/l
STOCK SOLUTION AND DILUTION
The test was performed with an aqueous extract (filtrate) of
the test substance (water solubility < 1mg/l); TS stirred in
demineralized water for ca. 20h at 20°C, undissolved TS
removed by membrane filtration (pore size 0.2 µm), nominal
concentration of the filtrate 125 mg/l, further dilution to
nominal concentrations: 100, 50, 25, 12.5, 6.25, 3.13, 1.56
mg/l.
TEST MEDIUM
prepared according to guideline (see above)
PEFORMANCE OF THE TEST
test temperature 23°C, max. difference 2°C;
test vessels 250 ml Erlenmeyer flasks plugged with
siliconsponge caps; test volume 100 ml;
UNEP PUBLICATIONS 27