C.I. Pigment Brown 24 CAS N°: 68186-90-3

OECD SIDS C.I. PIGMENT BROWN 24
TOXICITY ID 68186-90-3
DATE: 26-NOV-2004
5.0 Toxicokinetics, Metabolism and Distribution
Type:
Toxicokinetics
Species:
rat
No. of animals, males: 5
No. of animals, females: 5
Doses, males: 10, 100, 1000, 10000 mg/kg diet (0.5, 5, 50, 500
mg/kg bw/day)
Doses, females: 10, 100, 1000, 10000 mg/kg diet (0.5, 5, 50, 500
mg/kg bw/day)
Route of administration: oral feed
Exposure time:
90 day(s)
Method: other:comparable to OECD guideline 408
GLP:
no data
Remark:
Result:
The detected traces of antimony (see results) most likely
originate from the acid-soluble impurities of the pigment
(10-20 mg antimony/kg pigment, that is 5-10 microgram/kg bw or
125-250 microgram/kg organ weights at the highest dose) and
therefore do not indicate bioavailability of the pigment
itself.
TOXICICITY
No deaths, no overt signs of reactions to the treatment, no
effects on body weight gain (similar food consumption in all
groups) or organ weight, no treatment related findings
from haematological or biochemical investigations and
urinalysis.
No macroscopic pathological changes attributable to
treatment. No treatment related effects observed in
histopathology.
CHEMICAL ANALYSIS
Antimony
In males and females the Sb concentrations in liver and
kidney were below the detection limit at doses up to 1000
ppm. In the high dose groups the Sb levels slightly
increased with exposure duration and reached max. 27 ppb in
the liver (3 mo) of males (range 15-40 ppb) and 17 ppb in
females (kidney 14 ppb in males and 15 ppb in females).
Chromium
No measurable effect on chromium content of liver and kidney
at any dose level and exposure duration.
Test condition:
AUTHORS CONCLUSION
The bioavailable traces of metals are considered to have no
toxicological significance.
EXPERIMENTAL DESIGN
15 animals per dose per gender for toxicological
investigations and 30 animals per gender in the control
group;
additionally 10 animals per dose and gender for analytical
investigations and 20 animals per gender in the control
group.
Tests started at the age of 4- 5 weeks; TS given in powdered
food (Altromin); feed and tape water ad libitum.
General observations:
Rats observed daily; food consumption and body weight gain
determined once per week.
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