Dairy Insider - Thermo Fisher

Volume 1 Jun/Aug 2013
Insider
1300 735 292

welcome
Welcome to the inaugural issue of
the Dairy Insider. The team at Thermo
Fisher Scientific are thrilled to bring you
a publication focussed on the unique
needs of the dairy industry.
In this issue, we bring to you information on some of our latest
products including testing systems for ATP, pathogens, viscosity and
waste monitoring
Cassandra Anderson
Category Manager
Microbiology
The team at Thermo Fisher Scientific values customer feedback and
we’d love to hear your comments on the Dairy Insider. Please email us
at
with your comments.
Thank you for taking time to read Dairy Insider. We look forward to
hearing what you think. In the meantime, happy reading!
contents
The Science Behind Neogen’s
AccuPoint ® ATP Sanitation Monitoring System
Adenosine triphosphate (ATP) is found in every living cell. Since most of the food
and drink we consume was at some point living, ATP is present in most residue that
remains after a food contact surface is cleaned..........................................
Zero in on Listeria
with 3M Petrifilm
In the past, testing alternatives were usually too expensive. Therefore, very few facilities
were able to perform Listeria testing frequently and consistently enough to make
actionable decisions based on results.........................................................
Introducing you to the Microbiology team
- combining unique strengths to bring you
expertise, experience & innovation.
Thermo Fisher Scientific offers microbiologists a broad portfolio of industry-leading
brands, enabling us to offer you innovative workflow solutions. Some of the
brands included in our range include Oxoid TM , Remel TM , Trek Diagnostics TM , 3M TM ,
Qualicon TM and Idexx TM .
Supporting the comprehensive range of market-leading brands is the Microbiology
team. All members of the team have Microbiology laboratory experience and
product expertise, and can therefore assist and support you in your technical needs.
Please free feel to call us on 1300 735 292 if we can be of assistance to you.
Setting the Standards
in Viscosity Testing
The explosive growth in spreadable butters and low-fat substitutes over the last decade
is testament to the fact that spreadability is a key determinant in consumer acceptance
and convenience.......................................................................................
Product & Profit
Going Down the Drain...?
Spills, product changes and production issues at dairies and milk and cheese
processing plants can result in profits being literally washed down the drain. Add to that
the cost of treating sudden high carbon-load waste and the potential ..........
2
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3

At Every Link in the
Dairy Food Chain
Thermo Fisher Scientific is at the forefront of food safety and testing,
combining technical expertise and support with innovative products that
analyse, inspect and verifiy dairy ingredients and products, from point of
origin to the point of consumption. Our solutions strengthen the entire food
chain so you can deliver the uncompromised dairy products that consumers
expect and deserve.
• For more information visit www.thermofisher.com.au or call 1300-735-292
Laboratory Consumables Microbiology
Laboratory Equipment Life Science Chromatography Environmental & Industrial
• Blender Bags
• Chemicals
• Filter Papers
• Glassware
• Loops and Spreaders
• Petri dishes
• Tubes and Bottles
• Allergen kits
• Antibiotic residue test kits
• ATP – sanitation monitoring
• Dehydrated culture media
• Instrumentation
• Food Borne Pathogen Detection
(including molecular methods)
• Petrifilm TM
• Plates and Tubes
• Pre poured media & diluents
• Swabs
• Autoclaves
• Balances
• Biosafety Cabinets
• Centrifuges
• Cold Storage
• Incubators
• Ovens
• Stirrers
• Waterbaths
• Water Purification
• Filtration
• Immunoassays
• Liquid Handling
• Molecular Biology Reagents
• PCR & QPCR Instruments,
Reagents & Consumables
• Titration
• Accelerated solvent extraction (ASE)
• Chromatography solvents
• Gas chromatography – GC, GCMS
• Ion chromatography
• Liquid chromatography – HPLC, LCMS
• Protein separation columns
• Syringe filters
• Solid phase extraction
• Tubing & fittings
• Vials & closures
• Air quality monitoring
• COD (Chemical Oxygen Demand)
• Data logging
• Dust monitoring
• Heat stress monitoring
• Methane gas monitoring
• Noise monitoring
• Odour monitoring
• pH, conductivity, salinity, turbidity
4
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5

The Science Behind Neogen’s
AccuPoint ® ATP Sanitation Monitoring System
Author:
Robert Artuso
International Sales
Manager Neogen
Corporation
bartuso@neogen.
com
Adenosine triphosphate (ATP) is
found in every living cell. Since
most of the food and drink we
consume was at some point living,
ATP is present in most residue
that remains after a food contact
surface is cleaned.
ATP is also present in bacteria as well
as yeast and mold or other spoilage
organisms.
For this reason, the measurement of ATP
levels on a food contact surface or within
a water sample has become the de-facto
standard food and beverage producers
utilise to determine the cleanliness levels
of their operations.
Buffer
The AccuPoint Sampler
Luciferase and luciferin
on reagent pad
ATP is collected
on the sample pad
ATP from the sample pad
combines with the reagents
and a chemiluminescent
reaction begins
What ATP systems can’t do
ATP systems are not designed for total plate counts, or
detecting food allergens.
ATP results do not correlate with
microbial counts
A common misconception is that the results received from
ATP testing systems in relative light units (RLUs) for surface
samples, for example, should in some way correlate with a
microbial total plate count result for the same samples.
The amount of ATP in microbial cells can vary dramatically
and a single CFU can actually be comprised of one cell or
many cells. Bacteria need food to grow, and therefore, there
will never be an occasion where there is only microbial ATP
on a surface.
In theory there is a correlation between CFU levels and RLU
units; however, it is impossible to determine whether the
RLU results are due to the presence of bacteria, yeast, mold
or food residue. Most probably, it is a combination of these
things.
ATP systems are not direct indicators of bacterial presence;
however, they are the fastest and most convenient method
for determining the potential for these organisms to exist
on your food contact surface - therefore an indication of
how clean your surfaces are. There is simply no faster or
easier way to know that you could have a problem or that
certain areas in your facility require more vigorous cleaning.
Therefore, the issue can be corrected before it becomes a
serious problem.
ATP testing is a poor tool in an
allergen control program
Another misconception is the use of ATP sanitation
monitoring systems in a food allergen control program. The
theory here is that if all or most of the ATP on a surface or
in a clean-in-place (CIP) system has been eliminated, all
of the possible allergenic protein has also been eliminated.
This is not the case as 1) An allergenic protein can exist on
a surface at up to 100 times the level of concern and still be
below the detection level of any ATP system; and 2) You can’t
know how much of an ATP result is from an allergenic protein
and how much is from something else.
So why use an
ATP monitoring system?
»
»
The purpose of ATP testing is to achieve and defensively
document effective cleaning by following the principle
that if biomass is not present on surfaces after cleaning
there is not enough medium for microbial growth.
Properly utilised, sanitation monitoring systems allow for
the almost instantaneous detection and measurement
of ATP on food contact surfaces, which provides an
objective, actionable tool for the monitoring of a facility’s
sanitation efforts.
Exclusive Dairy Insider Special
For a limited time only the AccuPoint 2
ATP Sanitation Monitoring system is $3600
saving you $500 on upfront investment.
This package includes software, USB data
cable, battery charger, mobile carry case
and training DVD.
*Hurry this offer ends 31st August 2013.
Quote promo code NEO9602
Call 1300 735 292 to place your order or go to
www.thermofisher.com.au/godirect to order online.
$3600 *
NEO9602
6
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1300 735 292
7

Zero in on Listeria
with 3M Petrifilm
You can’t afford not to do it!
3M Petrifilm Environmental
Listeria Plates: the better way
to manage Listeria
In the past, testing alternatives were usually too expensive.
Therefore, very few facilities were able to perform Listeria testing
requently and consistently enough to make actionable decisions
based on results. This resulted in thousands of dollars spent on
environmental Listeria tests that were not actionable.
By implementing control procedures and increased testing
of environmental Listeria as an indicator, you can reduce
Listeria monocytogenes contamination. The 3M Petrifilm
Environmental Listeria plate is a powerful tool that will help you
manage Listeria.
Test more often.
By testing more consistently you can monitor the levels
of Listeria in different parts of your plant. The 3M
PetrifilmEnvironmental Listeria Plates is cost effective,
allowing you to test more often than ever before without
increasing your budget.
Monitor Listeria levels.
With consistent, frequent testing, the 3M Petrifilm
Environmental Listeria Plate’s quantitative results will allow you
to monitor any significant changes in environmental Listeria
levels and take informed and precise action.
The right choice for your facility.
The 3M Petrifilm Environmental Listeria Plate is a proven
and cost-effective tool for the detection of environmental Listeria.
• Quantitative results allow you to identify hot spots in the
plant and track your progress against Listeria over time.
• Rapid results in 27-31 hours from sample collection.
• Safer method - no enrichment, no culture transfers.
• Easy to use 3M Petrifilm Plate Technology.
Now, with the availability and cost-effectiveness of the
3M Petrifilm Environmental Listeria Plates, tracking and
managing Listeria is easy.
Zero in on the source of Listeria
Because Listeria is an organism that occurs naturally in the
environment, there are plenty of opportunities to continually
reintroduce it into the processing plant. One effective way to
control Listeria is by monitoring the levels of environmental
contamination in different parts of the plant.
By identifying and eliminating Listeria away from your
production you can minimise your risks.
ZONE 4
ZONE 3
Remote: eg. doorways, halls, drains
Non-Food Contact: eg. table legs, floor
Non-Food Contact: eg. equipment panels, aprons
ZONE 2
ZONE 1
Perform more
environmental tests here
Food Contact: eg. utensils,
conveyor, chutes, people
To prevent food
contamination here
How does a qualitative result help?
When Listeria is found within a processing environment
the source can often be traced back to a single point or
“hot spot” from which the contamination has spread. To
effectively control the threat of Listeria these hot spots must
be identified and to do this, information is vital.
Consider the two examples showing the results of
environmental sampling surrounding a food contact surface.
The qualitative results are unable to provide the information
needed to make an informed decision about the likely
location of the hot spot.
By using a quantitative method as shown, a more effective
response may be undertaken by zeroing in on the hot spot
faster and allowing for more thorough treatment or change to
prevent repeat occurrences.
Plating Technique Video
Qualitative
Quantitative
Current
Method
3M Petrifilm
Environmental
Listeria (EL)
Plates Method
Contact surface
sample point
Sample Sample using a using swab a or swab sponge or sponge
Inner near
sample point
Inner far
sample point
- + - + -
- - - - +
- - - + -
- - - - +
- - + + -
0 5 0 3 0
0 0 0 0 7
0 0 0 2 0
0 0 0 0 24
0 0 85 TNTC 0
Get results in 27-31 hrs from
sample collection
Traditional Listeria testing methods require two or more
days before your test is complete, allowing Listeria to go
unchecked while you wait for the result.
Add buffered Add buffered peptone peptone water. water.
Wait one Wait hour. one hour.
The 3M Petrifilm Environmental Listeria Plate result
is ready by the following day, giving you the power to make
faster decisions and react to problem areas.
Perform Listeria Tests with a safer method than enrichment.
Unlike traditional methods, the 3M Petrifilm
Environmental Listeria Plate requires no enrichment or
culture transfers. This means less risk of cross-contamination
in your facility.
Detect multiple Listeria species.
The 3M Petrifilm Environmental Listeria Plate allows
you to detect the most prevalent environmental Listeria
species, including Listeria monocytogenes.
Current Method vs. 3M Petrifilm Environmental Listeria Plates
Day 1 Day 2
10 am
Take sample
and perform
enrichment
Enrichment
time ranges
from 24-72
hrs
10 am 4pm
Sample
and test
with Petrifilm
EL Plate
Result
Ready
Day
3
Day
4
Testing time
ranges from
1-48 hrs
Fast, Easy and more informative
Sample Sample using a using swab a or swab sponge or sponge
Inoculate Inoculate and incubate and incubate
for 26-30 for hours 26-30 hours
Add buffered Add buffered peptone peptone water. water.
Wait one Wait hour. one hour.
Read and Read interpret and interpret
3M Petrifi lm Environmental
Listeria Plates
More informative results than presence/absence tests
3M Petrifi lm Environmental Listeria Plate provides you
with the information to take the appropriate action.
By using the quantitative results from 3M Petrifi lm
Environmental Listeria Plates as part of your overall control
plan against Listeria, you can know exactly where to target
your actions to best detect, manage and prevent Listeria in
your environment.
3M Petrifilm Environmental Listeria Plate
Sample A Sample B Sample C
Traditional Presence/Absence Test
Sample A Sample B Sample C
(-) (+) (+)
Order Now!
$580
Item Code Description UOM
3M6447-SU Environmental Listeria Start Up Kit:
- 3M6447 Petrifilm Environmental Listeria plates 50pcs
- 3M6432 Quick Swab 50pcs
- 3M6481 Petrifilm count plate spreader
- TM1453 Buffered Peptone Water 25mL pk/10
EA
Inoculate Inoculate and incubate and incub
for 26-30 for hours 26-30 hours
Authorised Distributor
8
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9

Setting the Standards
in Viscosity Testing
R/S-SST Plus Rheometer
Soft Solids Tester for pastes, slurries and materials with
particulates
Butter & Margarine
Spreadability
The explosive growth in spreadable
butters and low-fat substitutes over
the last decade is testament to the fact
that spreadability is a key determinant
in consumer acceptance and
convenience.
The spreadability of a product is a
function of it’s yield stress, the stress
that is required to overcome the
elastic structure present in a product
and cause it to flow. The SST Soft
Solids Tester offers an excellent way
of measuring such products quickly,
simply and reliably.
The Soft Solids Tester uses a vaned spindle that is lowered
into the sample to a pre-set insertion point prior to taking a
measurement. The vane has two big advantages for testing
such products:
1. There is very little damage to the sample’s structure during
vane insertion so we measure the sample as it really is,
2. Yellow fats products are notoriously prone to slippage
when in contact with smooth and shiny surfaces such as the
cones and plates of rheometers. When a vane the moving
element is effectively made of the sample itself and so
slippage is virtually eliminated.
A typical test method for spreadability assessment is to
insert a V20-10 vane (20mm high by 10mm across) into
the sample, rotate it at a predetermined rate of 0.5 rpm
and record the stress across the sample every second.
The results of this test performed on two samples at 6´C,
a traditional butter and it’s newer spreadable version are
shown in Figure 1.
The higher yield stress of traditional butter is immediately
obvious. The Soft Solids Tester’s analysis software reveals
these values to be 10287 Pa for the traditional and 3636
Pa for the spreadable version. This information is of obvious
value when we wish to set benchmarks for our desired
product qualities.
Figure 2 shows a comparison of two competing
spreadable butters and a margarine. A rounder peak and
less pronounced drop in stress after the yield point is
characteristic of ‘butteryness’ in a product. The sharp peak of
margarine indicates a certain ‘brittleness’ in the structure.
The Soft Solids Analysis software for the SST gives us yield
values of:
• Butter 1 3636 Pa
• Butter 2 1861 Pa
• Margarine 2937 Pa
The Soft Solids Tester can output these results numerically,
via the onboard lcd display; or graphically via the RS-SST’s
control and analysis software. For quality control usage Pass/
Fail tolerance bands can be set up for each product giving an
immediate visual screening of tested batches.
Stress [Pa]
Stress [Pa]
12000
10000
8000
6000
4000
2000
Traditional butter
Spreadable butter
0
0 10 20 30 40 50 60 70
Time [s]
Figure 1
4000
Spreadable butter 1
3500
3000
2500
2000
Margarine
1500
1000 Spreadable butter 2
500
0
0 10 20 30 40 50 60 70
Time [s]
Figure 2
Figure 1
The results of this
test performed on
two samples at
6´C, a traditional
butter and it’s
newer spreadable
version
Figure 1
A comparison of
two competing
spreadable butters
and a margarine.
Measured Values
- Yield Stress
- Shear Modulus
- Recovery
- Creep
Quantifies meaningful properties like stiffness,
wobbliness, sloppiness, consistency and texture
Capable of measurements in BU units for highly
viscous materials such as joint compound for gypsum
Vane Spindle Geometry
• Quick-Connect coupling
• Easy-to-test method
• Allows spindle insertion without compromising
sample structure
Coaxial Cylinders can also be used for complete flow
curve analysis
Smooth Height Adjustment for easy insertion of
spindle into sample without disrupting structure of sample
Need more information?
Call our Industrial Process team on 1300 735 295 or email
10
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11

With the
QuickCODultra
the analytical area
is isolated from
the electronics
so that not even
a leakage can
cause a damage.
All areas are easily
accessible.
Product &
Profit
Spills, product changes and
production issues at dairies and
milk and cheese processing
plants can result in profits being
literally washed down the drain.
Add to that the cost of treating
sudden high carbon-load waste
and the potential resultant high
discharge-to-sewer costs and
it makes sense to use reliable
online analysers to monitor the
process.
Waste Monitoring
Waste discharge from production, whether due to a spill
or due to the changing of products, results in a high COD
load for waste treatment. If the COD peak can be detected,
decisions can be made regarding its processing:
• A high COD load treated by an on-site DAF unit, for
example, may overload the treatment process. Switching
the waste flow to an offline buffer tank, to then feed
back to waste once the peak has passed, provides a
balanced flow to the DAF.
• Monitoring at the point of discharge-to-sewer can
provide details of COD loads for discharge cost
purposes.
• And if a high COD load is detected at discharge, the
waste can be diverted for re-treatment rather than
discharged, so saving on discharge costs.
• In addition, detecting the COD peak at various points
in the process can pin point where/why the peak is
occurring and reduce loss of product and the associated
costs
Detect with QuickCODultra
QuickCODultra, from LAR Process Analysers, is a rugged,
reliable online COD analyser, designed for these challenging
dairy applications. With a measuring range of 0.1mg/l
Going
Down
the Drain...?
to 200,000mg/l, the analyser is suitable for monitoring
raw waste from production, even with fats and grease, or
measuring the clean treated effluent.
In contrast to the common standard measuring methods,
which are time consuming and require hazardous reagents,
QuickCODultra uses the thermal combustion method. A
temperature of 1200°C ensures complete chemical digestion
of all compounds in the sample, including any particles and
without the need for the expensive catalysts used by lower
temperature analysers. Oxygen consumption is measured
using a zirconium oxygen sensor in order to provide a true
online COD measurement. Even with changing sample
matrices, the analyser delivers reliable and accurate results.
Ultra quick measurement and
maintenance
The COD measurement takes place in less than two minutes.
Thereby, rapidly changing peaks can be shown reliably. The
maintenance required is also quick: less than 30 minutes per
week. Moreover, all areas of the analyser have been designed
for easy maintenance: from filter-less sample extraction
with the patented FlowSampler ® , through the large and
blockage-free tubes, to the catalyst-free high temperature
oven with the removable oven foot for the quick removal of
salt residues.
Measurement Principle
The analysis cycle has three steps, per the schematic below:
1. Sample Transport, via robot X-Y movement
a. Extraction of the sample from the sample overflowcollecting
vessel
b. Injection of the sample into the furnace oven
c. Rinsing of the injection needle
2. Sample Combustion in the oven
3. Oxygen measurement by Zirconium Oxygen Sensor
MEASUREMENT PRINCIPLE
of the QuickCODultra
Carrier Gas
2
Combustion
Two Zone Oven
b
1.200°C
Cooler
Options
The following options are available:
• Up to six sample streams
• Additional sensors for TOC measurement and TN
(total nitrogen) measurement
• High salt option up to 300g/l
• Stainless steel cabinet, ATEX Zone1, Zone2
ADVANTAGES & FEATURES
Exact determination of the real COD
Proven thermal oxidation principle
Highest combustion temperature available (1,200°C)
Catalyst-free
Clean measurements without reagents
Multi-stream measurements (optional)
High salt concentrations of up to 100 g/l
Individually programmable user levels
Analyser availability minimum 98%
Maintenance and service maximum 30 minutes per week
Exceptionally low maintenance and operational costs
Injection Module
c
Rinsing
Vessel
Standard
Samples
1–6 Sample Collecting Vessels
(according to sample streams)
Acid Trap Air Filter O 2 -Detector Detectors
3
a
Measurement
Need more information?
Call our Environmental and Industrial Process team on 1300 735 295 or email
1
12
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13

July-September 2013
OUT NOW!
Lab Essentials
For every step of the way.
Microbiology Edition.
bringing more
to microbiology
Microbiological media are vital to the recovery and health of
patients and consumers. Thermo Fisher Scientific manufacture
routine and specialty media which are available every day for
every need.
Browse our July-September 2013
edition of Lab Essentials, focusing on
essential microbiology consumables for
your laboratory.
to view the
brochure or call 1300 735 292 to request
a copy.
Australia: For customer service, call 1300-735-292
Visit us online at: www.thermofisher.com.au
New Zealand: For customer service, call 0800-933-966
Visit us online at: www.thermofisher.co.nz
©2013 Thermo Fisher Scientific Inc. All rights reserved.
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