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BIODATA<br />

NAME<br />

Designation<br />

Corresponding<br />

Ad<strong>dr</strong>ess<br />

Ad<strong>dr</strong>ess<br />

DR. A. K. TIWARI<br />

Young Scientist (DST-SERC)<br />

Central Lab,<br />

U P Council of Sugarcane Research<br />

Shahjahanpur- 242 001, U P, INDIA<br />

C/O D N Tiwari,<br />

Mathia, Hetimpur, Deoria<br />

U. P., INDIA.<br />

Phone +91-5842-222509<br />

Mobile No : +91-9889297599<br />

E-mail<br />

ajay_biotech2005@rediffmail.com<br />

ajju1985@gmail.com<br />

Date of Birth 1 st of May 1985<br />

Academic Qualifications<br />

Exam Univ./ Board Year Div / Subject Remarks<br />

Passed<br />

%<br />

10 th U P Board 1998 1 st /74 Science 3 rd position<br />

in District<br />

12 th U P Board 2000 1 st / 64 Biology 1 st position<br />

in college<br />

B. Sc. Gor<strong>ak</strong>hpur University 2003 2 nd /55 Biology --<br />

M. Sc. Rajasthan Vidyapeeth 2005 1 st / 71 Biotechnology --<br />

University<br />

Ph. D. Ch. C.S. Univ. Meerut 2011 Biotechnology --


TITLE OF THE THESIS :<br />

Molecular Characterization of Viruses Occurring on Cucurbitaceous Crops in Eastern<br />

Uttar Pradesh.<br />

RESEARCH EXPERIENCE (Around 7 years)<br />

Since, August 2005, I have been working on Molecular characterization of <strong>Plant</strong> Viruses,<br />

Phytoplasma and improvement of Sugarcane crop through Tissue culture.<br />

DETAILS OF TRAINING AND RESEARCH EXPERIENCE<br />

1. Around Two years experience as Research Associate in Central Lab, UPCSR,<br />

Shahjahanpur from June 2011 to February 2013.<br />

2. Three years worked as Senior Research Fellow in Division of Tissue Culture, UPCSR,<br />

Shahjahanpur from July 2008- June 2011.<br />

3. Attended 21 days DBT Sponsored training programme held at IVRI Mukteswar, Nainital.<br />

4. Attended two weeks training programme in NBRI Lucknow on DNA isolation and PCR<br />

assays of plant viruses and phytoplasmas.<br />

5. Two years research experience on Characterization of <strong>Plant</strong> viruses at Department of<br />

Botany, DDU Gor<strong>ak</strong>hpur University in collaboration with SRS, Gor<strong>ak</strong>hpur, UP, India.<br />

6. Attended three months training programme at Sugarcane Research Station Gor<strong>ak</strong>hpur,<br />

U.P. (August 05-Oct 2005).<br />

7. Attended one month Training on Tissue culture of Sugarcane, at Sugarcane Research<br />

Station, Seorahi, Kushinagar , U.P. in 2005.<br />

8. Attended two weeks training on Microbiology at BRD Medical College, Gor<strong>ak</strong>hpur, U.<br />

P. in 2004.<br />

METHODOLOGIES AQUAINTED WITH :<br />

(MOL. BIOL. AND TISSUE CULTURE)<br />

a. DNA isolation from plant infected with virus and phytoplasmas<br />

b. PCR/Nested PCR<br />

c. Gel Electrophoresis<br />

d. Micropropogation of Sugarcane<br />

e. Crop improvement through somaclonal variation


BIOINFORMATICS TOOL KNOWN<br />

a. BLAST (NCBI)<br />

b. Sequence analysis through Genomatix Dialign Tool<br />

c. Phylogenetic analysis through MEGA-4.0 Version<br />

d. CLUXTAL-W<br />

e. Recombination detection analysis by RDP Version-2.0<br />

f. Batch Primer 3<br />

AWARDS<br />

1. Young Scientist Award by SERC, DST-2013, New Delhi, India<br />

2. Young Researcher Award by CIPAM-2011, Cagliari, Italy.<br />

3. Narshiman Award 2011 by Indian Phytopathological <strong>Society</strong>, MEZ at Kanpur<br />

University, U P.<br />

INTERNATIONAL TRAVEL AWARDS BY<br />

1. Patholux 2012, Luxemburgs.<br />

2. Department of Science & Technology (DST), New Delhi, India.<br />

3. Department of Biotechnology (DBT), New Delhi, India.<br />

4. Council of Scientific and Industrial Research (CSIR), New Delhi, India.<br />

PUBLICATIONS<br />

Research Paper Published in Journals 31<br />

Review Article in Journals 04<br />

Book Chapter Published 02<br />

Full Paper in Proceedings 01<br />

GenBank Submission 19<br />

Conference Attended 15


RESEARCH ACTIVITY<br />

1. Invited Lecture: a). S. P. P. G. College Shohratgarh, Siddharthnagar, Uttar<br />

Pradesh, India.<br />

b). GF College, Shahjahnapur, Uttar Pradesh, India.<br />

2. Scientific Visits: Italy, Germany, China<br />

3. Students Guided: 09 M. Sc. Students guided and 39 B. Sc. students trained<br />

4. Regular Reviewer : a). Medicinal <strong>Plant</strong>s<br />

b). Global Journal of Microbiology<br />

c). African Journal of Agricultural Sciences<br />

d). Journal of <strong>Plant</strong> Breeding and Crop sciences<br />

e). Journal of Crop Improvement<br />

f). African Journal of Biotechnology<br />

g). Sugar Tech<br />

h). e Journal of <strong>Plant</strong> <strong>Pathology</strong><br />

5. Member of Editorial Board/Associate Editor<br />

a). Medicinal <strong>Plant</strong>s<br />

b). e Journal of <strong>Plant</strong> <strong>Pathology</strong><br />

c). European Journal of Biology<br />

d). Agrica<br />

6. Memberships: a). Senior member of APCBEES (Asia pacific Chemical, biological &<br />

Environmental Engineering <strong>Society</strong>).<br />

b). Vegetos Journal (Annual member of <strong>Society</strong> of <strong>Plant</strong> Research, Meerut,<br />

U P).<br />

c). Sugar Tech (<strong>Society</strong> of Sugarcane Research and Promotion, GKP, UP)<br />

d). India Phytopathology (Indian Phytopathological <strong>Society</strong>, New Delhi)<br />

e). Medicinal <strong>Plant</strong>s (<strong>Society</strong> of Conservation and Resource Development<br />

of Medicinal <strong>Plant</strong>s)<br />

f). Indian Botanical <strong>Society</strong> (Annual member)<br />

g). STAI (Sugarcane Technologist Association of India MN 1495)<br />

h). ISSCT (International <strong>Society</strong> of Sugarcane Technologists MN 530)<br />

i). Prof H S Srivastava <strong>Society</strong>, Lucknow, UP.


REFERENCES<br />

Dr. S. K. Raj<br />

Scientist G and Group Leader<br />

Molecular Virology Lab<br />

National Botanical Research Institute,<br />

Rana Pratap Marg, Lucknow-226001, India<br />

Mobile: +91 9415091366<br />

Email: skraj2@rediffmail.com<br />

Dr. P. K. Sharma<br />

Associate Professor/Head<br />

Department of Microbiology<br />

Ch C S University, Meerut, India.<br />

Mobile. +91 9412781914<br />

Email: pks264@rediffmail.com<br />

Dr. G. P. Rao<br />

Principal Scientist<br />

Division of Mycology and <strong>Plant</strong> <strong>Pathology</strong><br />

Indian Agricultural Research Institute<br />

Pusa Campus, New Delhi<br />

Mobile: +91 9839293383<br />

Email : gprao_gor@rediffmail.com<br />

Dr. S. S. Solmon<br />

Director<br />

Indian Institute of Sugarcane Research<br />

Lucknow, India<br />

Mobile:+91-9839040995<br />

Email: <strong>dr</strong>solomonsushil1952@gmail.com<br />

Declaration:<br />

I hereby declare that all the information furnished by me is true to the best of my<br />

knowledge and if any discrepancy is found, I am ready for cancellation of my candidature.<br />

Date:<br />

Place: Shahjahanapur<br />

(A. K. TIWARI)


LIST OF PUBLICATIONS<br />

Research Articles in Journals<br />

1. A K Tiwari, S K Vishw<strong>ak</strong>ama, P Kumar, S P Singh, M S Khan, C Chun and G P Rao.<br />

First report of Candidatus Phytoplasma affecting Ageratum conyzoiodes in India. <strong>Plant</strong><br />

<strong>Pathology</strong> (New Disease Report) (2012) 26:pp 18. IF 2.25, NAAS 8.0.<br />

2. A K Tiwari, S Tripathi, M Lal and S Mishra, Chemical sterilization of nutrient medium<br />

for in vitro micropropogation of sugarcane (2012) Sugar Tech 14 (4) 364-369. NAAS<br />

5.0.<br />

3. P Saxena, S K Vishwkarma, A K Tiwari, A Singh and A Kumar, Pathological and<br />

Molecular variation in C. falcatum isolate in sugarcane (2013) Journal of <strong>Plant</strong><br />

Protection Research 53 (1) 1-5.<br />

4. A K Tiwari, S K Vishw<strong>ak</strong>arma and G P Rao, Increasing incidence of sugarcane grassy<br />

shoot disease in Uttar Pradesh and its impact on yield and quality of sugarcane.<br />

Phytopathogenic Molecutes (2012) 2(2) 63-67.<br />

5. G P Rao, A K Tiwari, M Singh, Y Chaturvedi and Madhupriya. Molecular<br />

Characterization and Phylogenetic Relationships of an Isolate of Candidatus phytoplasma<br />

asteris affecting Zinnia elegans in India. Phytopathogenic Molecutes (2012) 2(1) 33-36.<br />

6. S K Raj, S K Snehi, M S Khan, A K Tiwari and G P Rao First molecular characterization<br />

of Ageratum enation virus associated with mosaic disease of pointed gourd<br />

(Trichosanthes dioica Roxb.) in India, Phytoparasitica (2011) 39 (5) 497-502. IF 0.60,<br />

NAAS 7.0.<br />

7. M Singh, Y Chatruvedi, A K Tiwari, G P Rao, S K Snehi, S K Raj, M S Khan. Diversity<br />

among phytoplasmas infecting or<strong>name</strong>ntal plants grown in India. Bulletin of Insect logy<br />

(2011) 64: 69-70. IF 0.30, NAAS 6.8.<br />

8. S K Raj, S K Snehi, M S Khan, M Singh, Y Chaturvedi, A K Tiwari and G P Rao,<br />

Molecular detection and identification of phytoplsam associated with little leaf and witch<br />

broom disease of marigold in India. Phytopathogenic Molecutes (2011) 1 (1) 41-46.<br />

9. S Huang, A K Tiwari and G P Rao. Candidatus phytoplsam pini affecting T ascendens in<br />

China. Phytopathogenic Molecutes (2011) 1 (2) 91-94.<br />

10. A K Tiwari, S K Snehi, M S Khan, P K Sharma, S K Raj and G P Rao, Molecular<br />

detection and identification of Tomato leaf curl New Delhi virus associated with yellow<br />

mosaic and leaf curling disease of Luffa cylin<strong>dr</strong>ica crops in India, Indian<br />

Phytopathology (2012) 65 (1): 48-55. NAAS 3.8.


11. A K Tiwari, N. Mishra, S. Tripathi, M. Lal, R. K. Singh and M. L. Sharma. Assessment<br />

of genetic stability in micropropagated population of sugarcane variety CoS 07250<br />

through SSR markers. Vegetos (2011) 24 (2) 74-81. IF 0.039, NAAS 6.8.<br />

12. A K Tiwari, G P Rao, M S Khan, N Pandey and S K Raj. Detection and elimination of<br />

begomovirus affecting T dioica plant in India. Archives of Phytopathology and <strong>Plant</strong><br />

Protection (2012) 45 (9) 1070-1075. NAAS 4.0<br />

13. Pankaj Kumar, A Agarwal, A K Tiwari, M. Lal and MRA Jabri Development of red rot<br />

resistance in sugarcane through somaclonal variation. Sugar Tech (2012) 14 (2) 192-194.<br />

NAAS 5.0.<br />

14. A K Tiwari, S K Snehi, R Singh, S K Raj, G P Rao and P K Sharma, Molecular<br />

identification and genetic diversity among six begomovirus isolates affecting cultivation<br />

of cucurbitaceous crops in Uttar Pradesh, Archives of Phytopathology and <strong>Plant</strong><br />

Protection (2012): 45 (1) 62-72. NAAS 4.0.<br />

15. N Pandey and A K Tiwari, Identification of Zinnia leaf curl virus infecting Zinnia<br />

elegans in India. Journal of Biotechnology and Bioinformatics (2012) 2 (1) 6-10.<br />

16. S K Raj, S K Snehi, M S Khan, A K Tiwari and G P Rao, First report of Paper leaf curl<br />

Bangladesh virus affecting M. charantia in India. <strong>Australasian</strong> <strong>Plant</strong> <strong>Pathology</strong> (2010)<br />

5: 14-16. IF 1.00, NAAS 7.4.<br />

17. S K Raj, S K Snehi, M S Khan, A K Tiwari and G P Rao, First report of Ageratum<br />

enation virus affecting Cleome gynen<strong>dr</strong>a in India, Journal of General <strong>Plant</strong> <strong>Pathology</strong><br />

(2010) 76 (4) 292-294. IF 1.00, NAAS 7.0.<br />

18. M S Khan, C Chun, S K Raj, A K Tiwari and P Seth. First report of Indian Cassava<br />

mosaic virus on Chilli in India. Journal of <strong>Plant</strong> <strong>Pathology</strong> (2011) 93 (4) 89 IF 0.90,<br />

NAAS 6.8.<br />

19. A K Tiwari, S Tripathi, M Lal, M L Sharma and P Chiembostat. Elimination of<br />

Sugarcane phytoplasma through apical meristem culture. Archives of Phytopathology<br />

and <strong>Plant</strong> Protection (2011) 44 (20) 1942-1948. NAAS 4.0<br />

20. D Srivastva, N Pandey, A K Tiwari and K Shukla. Identification and characterization of<br />

a potyvirus associated with mosaic disease of Catharanthus roseus in Gor<strong>ak</strong>hpur, U.P.,<br />

India. Medicinal <strong>Plant</strong>s (2012) 4(1) 23-27. NAAS 3.6.<br />

21. A K Tiwari*, Deepmala Singh, S Tripathi, N Mishra, and R B Singh, Effect of BAP and<br />

Kn on shoot initiation of Trichosanthes dioica; an important medicinal plant, Medicinal<br />

<strong>Plant</strong>s (2010) 2 (1): 59-61. NAAS 3.6.<br />

22. A K Tiwari, P K Sharma, M S Khan, S K Snehi, S K Raj and G P Rao, Molecular<br />

detection and identification of Tomato leaf curl New Delhi virus isolate causing yellow


mosaic disease in Bitter gourd (Momordica charantia), a medicinally important plant in<br />

India, Medicinal <strong>Plant</strong>s (2010) 2 (2): 117-123. NAAS 3.6.<br />

23. Suman Mishra, Deepti Singh, A K Tiwari, M Lal and G P Rao, Elimination of<br />

Sugarcane mosaic virus and Sugarcane stre<strong>ak</strong> mosaic virus by Tissue culture,<br />

International Sugar Journal (2010) 28 (3):119-122. IF 0.14, NAAS 4.0.<br />

24. A K Tiwari, S Mall, M S Khan, S K Snehi, P K Sharma, G P Rao and S K Raj. Detection<br />

and identification of Tomato leaf curl Palampur virus infecting Cucurbita pepo in India.<br />

Gunaxi Journal of Agricultural Sciences (2010) 40 (12): 1291-1295.<br />

25. N Pandey, A K Tiwari and K Shukla. Detection and identification of Ageratum enation<br />

virus infecting Ageratum conzoides in India. Acta Phytopathologica et Entomologica<br />

Hungrica (2011) 46(2): 205-214. NAAS 4.0.<br />

26. S Path<strong>ak</strong>, M Lal, A K Tiwari and M L Sharma, Effect of growth regulators on in vitro<br />

multiplication and rooting of Sugarcane. Sugar Tech (2009) 11(1): 80-82. NAAS 5.0.<br />

27. Deepti Singh, A K Tiwari, G P Rao, R Karuppalah, R Viswanathan, Meen<strong>ak</strong>shi Arya<br />

and V K Barnwal, RTPCR/PCR analysis detected mixed infection of DNA and RNA<br />

viruses infecting sugarcane crops in different states of India. Sugar Tech (2009) 11 (4):<br />

373-380. NAAS 5.0.<br />

28. Y Chaturvedi, A K Tiwari, P P Uppadhaya and G P Rao, Association of Candidatus<br />

Phytoplasma Asteris with little leaf disease of Catharanthus Roseus in Eastern U. P.,<br />

India. Medicinal <strong>Plant</strong>s (2009) 1 (2): 103-108. NAAS 3.6.<br />

29. A K Tiwari, S Yadav, S Tripathi, J Rastogi, Y P Bharti, M Lal and M L Sharma,<br />

Performance of micropropogated, SCYLV infected and healthy plants of sugarcane.<br />

Indian J of Sugarcane Technology (2008) 23 (1&2): 41-44. NAAS 3.1.<br />

30. G P Rao, S Srivastava, P S Gupta, S R Sharma, A Singh, S Singh, M Singh, A K<br />

Tiwari and C Marcone. Detection of Sugarcane Grassy Shoot Phytoplasma infecting<br />

Sugarcane in India and phylogenetic relationship to closely related phytoplasmas. Sugar<br />

Tech (2008) 10 (1): 74-80. NAAS 5.0.<br />

31. Ashutosh Singh, A K Tiwari, P P Upadhayaya and G P Rao, Molecular Characterization<br />

and Coat protein sequence comparison of Papaya ringspot virus isolate from Uttar<br />

Pradesh, India, Indian J of <strong>Plant</strong> <strong>Pathology</strong> (2007) 25 (1&2): 64-68. NAAS 2.0.<br />

Review Articles in Journals<br />

32. Yamini Chaturvedi, A K Tiwari, G P Rao, B. Duduk and A. Bertaccini, Phytoplasmas on<br />

Or<strong>name</strong>ntal: Detection, Diversity and Management, Acta Phytopatthologica et<br />

Entomologica Hungrica (2010) 45 (1): 31-69. NAAS 4.0.


33. A K Tiwari, S K Vishw<strong>ak</strong>ama, N Pandey, P Kumar and M Lal. Ratoon Stunting Disease<br />

of Sugarcane. <strong>Plant</strong> Knowledge Journal (2012) 1 (1) 20-24.<br />

34. A K Tiwari, Y P Bharti, N Mishra, S Tripathi, M Lal, P K Sharma, G P Rao and M L<br />

Sharma, Biotechnological Approaches for Improving Sugarcane Crop with Special<br />

Reference to Disease Resistance, Acta Phytopatthologica et Entomologica Hungrica<br />

(2010) 45 (2): 235-249. NAAS 4.0.<br />

35. M S Khan, A K Tiwari. S H Ji and S C Chun. Ageratum conyzoides and Its Role in<br />

Begomoviral Epidemics; Ageratum enation virus: An Emerging Threat in India (2012)<br />

Vegetos 24 (2) 20-28. IF 0.039, NAAS 6.0.<br />

Full Research Articles Published in Proceedings<br />

36. Akshi Agarwal, Pankaj Kumar, A K Tiwari, M. Lal and M.R.A. Jabri. Optimization of<br />

hormonal requirements of the in vitro micropropogation of sugarcane. 4 th IAPSIT 2011<br />

held at New Delhi, India pp 800-801.<br />

In books (Chapters edited):<br />

37. S K Raj, S K Snehi, A K Tiwari and G P Rao, Biological, Molecular identification and<br />

Management strategies of Begomovirus infecting Cucurbitaceous crops in India, <strong>Plant</strong><br />

Viruses, (Eds G P Rao, B Mandal, V K Barnwal & N Rishi) Published from LLC Press<br />

USA (2010) Recent trades in <strong>Plant</strong> Virology (Eds. G.P. Rao, V. K. Baranawal, B. Mandal<br />

& N. Rishi), Studium Press LLC, USA pp. 135-155..<br />

38. M Lal, R S Senger, A K Tiwari, G P Rao and M L Sharma. Biotechnological<br />

Approaches for substainable crop productivity in sugarcane, Stable Food Production &<br />

Substainable Agriculture (Eds R S Senger and A K Sharma) Published from Studium<br />

Press USA pp 319-334.<br />

List of sequences submitted in GenBank Database:<br />

(Available at www.ncbi.nlm.nih.gov/sites/entrez)<br />

A. Begomoviruses:<br />

ACCESION NO.<br />

EU888908, EU867514, EU439261, EU439260, EU366164, EU366163, GQ412348,<br />

GQ845029, GQ268327.


B. Phytoplasmas:<br />

ACCESION NO.<br />

FJ 939287, FJ 939288, GU551936, GU594056. HM230687, HM230688, HM230684,<br />

HM230685, HM230686, JQ446367.<br />

Conference / Symposium / Seminar /Workshop<br />

1. STAI meetings held at Goa, India in 2008.<br />

2. Virocon 2008, New Delhi, India.<br />

3. Virocon 2010, Tirupati, AP, India.<br />

4. Virocon 2012, IVRI, Mukteswar, India.<br />

5. Medicinal <strong>Plant</strong> Conference held at Chennai in 2010.<br />

6. CIPAM-2011 held at Cagliari, Italy from 13-15 April 2011.<br />

7. Biodiversity conference held at Shoharatgarh, SDR, UP, India in<br />

2011.<br />

8. 4 th IAPSIT meetings held at New Delhi, India in 2011.<br />

9. Medicinal <strong>Plant</strong>s conference held at Gor<strong>ak</strong>hpur, UP, India in 2004.<br />

10. Biodiversity conference held at Gor<strong>ak</strong>hpur, UP, India in 2010.<br />

11. Zonal meeting of Indian Phytopathological <strong>Society</strong> held at Kanpur<br />

University 2011.<br />

12. 10 th <strong>Pathology</strong> workshop organized by ISSCT at Nanning,<br />

China from 7-11May 2012.<br />

13. STAI 2012, held at Hyderabad, India<br />

14. Indian Phytopatholoigcal Meeting held at BHU, Varanasi, UP,<br />

India.<br />

15. National Seminar on Sugarcane held at PUSA, Bihar in November<br />

2012.

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