64. Volume 10- Number 2 - IP Australia

PLANT VARIETIES JOURNAL 1997 VOL 10 NO. 2
Table 15 Neotyphodium strains
‘AR 501’ *‘Tf2’ *‘Tf28’ *‘FaTG-2’ C
(FaTG-3) B (wild type)
FaF coA, coB coC FaA, FaC, FaD,
FaD, FaE
CONIDIAL LENGTHS (µm) -range
5.5 – 7.2 D 6.0-15.0 7.0-10.5 4.5-7.5
ALKALOID PROFILE
ergovaline – + + +
peramine + + + variable
lolines + + + –
A – MJ Christensen, A Leuchtmann, DD Rowan and BA Tapper, 1993. Taxonomy of Acremonium endophytes of tall fescue (Festuca arundinacea), meadow
fescue (Festuca pratensis) and perennial ryegrass (Lolium perenne). Mycological Research 97: 1083-1092.
B – Festuca arundinacea taxonomic group three
C – Festuca arundinacea taxonomic group two (‘Tf13’, ‘Tf15’, ‘Tf20’, ‘Tf21’, Tf23 E )
Characterisation of Neotyphodium isolate ‘AR501’ using isozyme analysis. Three alleles are used to distinguish isolate
‘AR501’ from other strains of Neotyphodium endophytes that have been obtained from tall fescue -PGI: phosphoglucose
isomerase (=PHI); PGM: phosphoglucomutase; PGD= 6-phosphogluconate dehydrogenase (=6PG). Position of bands on gels
are indicated by R f values relative to reference strain ‘Tf2’. Isolates: ‘Tf2’(isozyme phenotypes coA and coB); ‘Tf28’ (isozyme
phenotype coC); ‘Tf21’(isozyme phenotype FaD) and ‘AR501’(isozyme phenotype FaF).
ENDOPHYTE – RYEGRASS
Neotyphodium (Acremonium*) lolii
‘AR1’
Application No: 97/013 Accepted: 6 Feb 1997.
Applicant: New Zealand Pastoral Agriculture Research
Institute Limited, Palmerston North, New Zealand.
Agent: A (Tony) Stratton, AgResearch Grasslands
(Australia), Albury, NSW.
Description (Table 16, Figure 46) Fungal isolate: seed
borne endophytic fungus mainly present in ryegrasses,
nonsporulating. It has unique allozyme of enzyme PGM,
RAPD (Random Amplification of Polymorphic DNA)
profile different from wild type strain (‘Nui 8’), lack of
production of the toxic secondary alkaloid metabolites
lolitrem B and ergovaline, and distinct colony
characteristics.
Origin Isolation and culturing: seeds of perennial ryegrass
collected in central Italy. Breeder: AgResearch Grasslands
Research Centre, Palmerstone North, New Zealand.
Selection criteria: lack of production of toxic secondary
metabolites lolitrem B and ergovaline and production of
only the beneficial alkaloid – peramine. Propagation:
asexual and hence uniform and stable.
Comparative Trials Comparators: Isozyme analysis:
‘Lp1’(endosafe®), ‘Lp8’(wild type N. lolii), ‘Lp12’(isozyme
phenotype). RAPD analysis: wild type N. lolii ; ‘AR501’
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