Abstracts - ISMST - International Society for Medical Shockwave ...

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14th congress of the ISMST June 9 th – 11 th 2011 Atlantic Hotel Kiel, Germany Institution: Orthopaedic Department, Ludwig-Maximilians-University Munich, Germany Device and producing company: XL 1, Dornier Introduction: The delayed healing or nonunion of bone is a common problem as it occurs in 5 - 10 % of all fractures. Hypertrophic non-unions need only to be stabilized and they will usually unite. But the treatment of the aseptic, atrophic nonunion is a clinically relevant problem, because of its biological inactivity. The current treatment is accompanied by an extended surgical procedure including resection of necrotic bone and bone grafting and therefore causing a large amount of stress, hospitalization and a prolonged work disability for the patient. Extracorporeal Shockwave Therapy (ESWT) is a possible non-invasive treatment, as recent clinical trials show. In basic research the application of extracorporeal shock waves has been shown to enhance new bone formation in healthy bone. Target cells are fibroblasts, osteoblasts and mesenchymal stem cells in the bone marrow. Therefore we investigated whether extracorporeal shock waves may differentiate mesenchymal stem cells into an osteoblastic lineage. Methods: Mesenchymal stem cells were cultured and treated by shock waves with 15 and 25 kV. Supernatant was investigated by ELISA for bone markers. Also expression of bone markers like cbfa1, collagen1 and ALP was examined by lightcycler. Histology was performed by a v. Kossa staining. Results: Histology showed a positive v. Kossa stain for the treated cells. In the group of 15 kV marked elevations in the expression of bone markers cbfa1, collagen1 and ALP were found. ELISA did show only slight changes in the production of bone markers. Discussion: The results reveal new insights in the mechanism of bone healing by extracorporeal shock waves. Until now, many authors have shown the production of bone growth factors by osteoblasts or fibroblasts, whether this leads to a differentiation or whether direct mechanical effects on the stem cells force them to differentiate has to be clarified in the future. Conclusion: Extracorporeal shock wave treatment of mesenchymal stem cells leads in vitro to a differentiation into an osteoblastic lineage. This may contribute to the healing of non unions. Name Abstract No. Abello, Sergio...................................................................................................................................................................... (26) Aharinejad, Seyedhossein ................................................................................................................................................. (19) Al-Kandary, Faisal .....................................................................................................................................................(10,11,44) Amelio, Ernesto ................................................................................................................................................................. (27) Antonic, Vlado .................................................................................................................................................................... (5) Arellano, Leonardo .............................................................................................................................................................. (4) Asopa, Vipin .... .................................................................................................................................................................. (55) Auersperg, Vinzenz ............................................................................................................................................................(14) Bätje, Frank.......................................................................................................................................................................... (25) Balabel, Ayman Elwy .................................................................................................................................................(10,11,44) Baron .................................................................................................................................................................................... (36) Bauermeister, Wolfgang .................................................................................................................................................... (48) Becker, Michael.................................................................................................................................................................... (3) Bellomo, Rosa Grazia ........................................................................................................................................................ (33) Bernardini, B. ..................................................................................................................................................................... (53) Berta, Laura....................................................................................................................................................................(16,32) Berta, Marina ...................................................................................................................................................................... (32) Bosco, Vincenzo ............................................................................................................................................................... (34) Branca, A. ......................................................................................................................................................................... (21) Brañes, Julian Andres ......................................................................................................................................................... (4) Brañes, Manuel ................................................................................................................................................................... (4) Buselli, Paolo ..................................................................................................................................................................(21,34) Cacchio, Angelo .................................................................................................................................................................. (9) 40
Abstracts Canaparo, Roberto............................................................................................................................................................ (16) Chang, Michael.................................................................................................................................................................... (1) Compagnoni, R. ................................................................................................................................................................ (21) Coombs, Richard .............................................................................................................................................................. (55) Cozzolino, A. ...................................................................................................................................................................... (45) d‘Agostino, Maria Cristina ................................................................................................................................................. (53) den Boer, Willem ................................................................................................................................................................(51) Diercks, Ron ..................................................................................................................................................................... (7, 8) Di Palma, L. ........................................................................................................................................................................ (21) Di Stefano, Alexandra......................................................................................................................................................... (33) Drmic, Monika .................................................................................................................................................................... (40) Duchstein, Hans-Jürgen ................................................................................................................................................... (17) Eandi, Mario ....................................................................................................................................................................... (16) Eid, José ..............................................................................................................................................................................(12) Everke, Heinrich ................................................................................................................................................................ (49) Fagnani, Filippo ................................................................................................................................................................. (52) Fischer, Andreas ............................................................................................................................................................... (28) Frairia, Roberto .............................................................................................................................................................(16,32) Fuente, Matias de la............................................................................................................................................................. (3) Furia, John Patrick .............................................................................................................................................................. (9) Galati, Valentina ................................................................................................................................................................. (33) Garilevich, Boris ................................................................................................................................................................ (15) Garilevich, Marina .............................................................................................................................................................. (15) Gasparroni, V. .................................................................................................................................................................. (53) Gerdesmeyer, Ludger ...................................................................................................................................................(13,47) Goeren, Tolga .................................................................................................................................................................. (56) Gollwitzer, Hans ............................................................................................................................................................(13,54) Grimm, Michael .............................................................................................................................................................(18,19) Guerra, Claudio ................................................................................................................................................................. (27) Guiloff Waisbluth, Leonardo ................................................................................................................................................ (4) Hafez, Moustafa ................................................................................................................................................................ (55) Haller, Gerd ........................................................................................................................................................................ (40) Hanna, Milad ..................................................................................................................................................................... (55) Hartgens, Fred .................................................................................................................................................................... (7) Hartinger, Joachim .............................................................................................................................................................. (5) Hartmann, Bernd .............................................................................................................................................................. (42) Hausdorf, Jörg .................................................................................................................................................................. (56) Hein, Marc ............................................................................................................................................................................ (3) Holfeld, Johannes .........................................................................................................................................................(18,19) Horwood, Nikki ................................................................................................................................................................. (55) Jansson, Volkmar .............................................................................................................................................................. (56) Joest, Beatrice ...................................................................................................................................................................(41) Karadas, Ender............................................................................................................................................................. (28,29) Kertzman, Paulo ................................................................................................................................................................ (31) Knobloch, Karsten ........................................................................................................................................................ (37,41) 41
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