Gilson Product Guide (PDF) - John Morris Scientific

Gilson, Inc. 

Table of Contents 

Designed for purification and engineered for science, Gilson, Inc. provides high quality, dependable solutions 

for today’s demanding liquid chromatography, solid phase extraction, liquid handling and gel permeation 

chromatography clean-up requirements. From the legendary Gilson Pipetman® products to a full range of system 

solutions, Gilson is the element of purification for your laboratory. Gilson’s innovative products will revolutionize 

the lab; providing the durability, flexibility, modularity and ease of use the industry has come to expect from Gilson. 

Gilson has worldwide sales and service supporting the pharmaceutical, biotech, environmental, clinical, food and 

forensic markets. Our primary focus is on purifying samples via preparative HPLC, automated SPE or through GPC 

clean-up. Gilson focuses on early drug discovery and development with our preparative HPLC systems. Gilson 

automated SPE systems and GPC clean-up systems support environmental, food, clinical and forensic markets 

performing a wide variety of clean-up applications. 

Gilson offers complete solutions including the revolutionary control software, Trilution®. Trilution® LC Software 

is a complete HPLC software package designed specifically to meet purification needs. Trilution® LH Software is 

designed to make automation of liquid handling and SPE applications simple and dependable. 

Gilson, Inc. world headquarters is located in Middleton, WI and is responsible for research, development and 

manufacturing of all Gilson instrumentation and software in addition to providing direct sales to the U.S. Gilson 

S.A.S., located outside of Paris, France, is responsible for all development and manufacturing of Gilson pipetting 

products and benchtop laboratory products. Gilson solutions are sold and supported worldwide. 

HPLC: Systems 2 – 9 

GX-281 Preparative HPLC System......................................... 5 

GX-281 Analytical to Semi-preparative 

HPLC System............................................................................ 6 

GX-271 Preparative HPLC System......................................... 7 

GX-271 Analytical to Semi-preparative HPLC System.... 8 

845Zprep Semi-preparative to 

Preparative HPLC System.................................................... 9 

cLC Analytical to Preparative HPLC System.................... 9 

HPLC: Liquid Handlers/Injectors 10 –14 

GX-281 Liquid Handler/Injector..........................................11 

GX-271 Preparative Liquid Handler/Injector...................12 

GX-271 Analytical Liquid Handler/Injector......................13 

GX-271 Liquid Handler/Injector without pump............13 

215 Liquid Handler/Injector..................................................14 

Multiple Probe 215 Liquid Handler/Injector...................14 

HPLC: Pumps 15 –19 

333/334 Prep-scale HPLC Pumps........................................16 

331/332 Semi-preparative HPLC Pumps..........................17 

321/322 Analytical to Semi-preparative 

HPLC Pumps..........................................................................17 

30X Series HPLC Pumps..........................................................18 

402 Syringe Pumps...................................................................18 

Minipuls Evolution® Peristaltic Pump................................19 

Minipuls 3 Peristaltic Pump...................................................19 

HPLC: Detectors 20 – 22 

171/172 Diode Array Detectors...........................................21 

15X Series UV/VIS Detectors.................................................21 

112 UV Detector........................................................................22 

prepELS II Detector..................................................................22 

HPLC: Fraction Collectors 23 – 25 

prepFC Fraction Collector.....................................................24 

FC 203B/FC 204 Fraction Collectors...................................25 

Control Software 26 – 28 

Trilution® LC Software..............................................................27 

Trilution® LH Software..............................................................28 

GPC Clean-up Systems 29 – 32 

Automated GX-271 GPC Clean-up System......................30 

GX-271 GPC/SPE Clean-up System.....................................31 

Manual GPC Clean-up System..............................................32 

Solid Phase Extraction Systems 33 – 37 

GX-271 ASPEC, with 406 Single Syringe Pump...........34 

GX-271 ASPEC, with 406 Dual Syringe Pump..............34 

GX-274 ASPEC, with two 406 Dual Syringe Pumps....35 

Quad-Z 215 Liquid Handler for SPE....................................36 

SPE 215 System..........................................................................37 

Code 300 Series Racks.............................................................37 

Liquid Handlers 38 – 45 

GX-281 Liquid Handler............................................................39 

GX-271 Preparative Liquid Handler....................................40 

GX-271 Analytical Liquid Handler.......................................41 

GX-271 Liquid Handler without pump..............................41 

GX-274 Liquid Handler............................................................42 

Quad-Z 215 Liquid Handler...................................................43 

Quad-Z 215 Liquid Handler with Disposable Tips........43 

Multiple Probe 215 Liquid Handler....................................44 

215 Liquid Handler...................................................................44 

223 Sample Changer...............................................................45 

Accessories 46 – 50 

VALVEMATE® II Valve Actuator.............................................47 

Orbital Shaker.............................................................................48 

RackCount System.................................................................48 

818 AutoMix...............................................................................49 

852/853/854 Peltier Racks.....................................................49 

242/542 Peltier Racks..............................................................50 

Racks - Standard and Custom...............................................50 

References 51 – 68 

Gilson Rack/Instrument Compatibility Chart........ 51 – 54 

Gilson Probe Selection Guide..................................... 55 – 58 

Periodic Table.............................................................................59 

Flow Cell Selection Chart.......................................................59 

pH of Common Acids and Bases.........................................60 

Column Sizes and Capacities................................................60 

Maximum UV Absorbance 

for Commonly Used Solvents..........................................61 

Optimizing Preparative HPLC...............................................62 

Separation Scale-up Equations............................................62 

Solvent Miscibility Table.........................................................63 

Solvent Polarity Chart..............................................................63 

Common Conversion Tables....................................... 64 – 65 

Gilson Applications........................................................ 66 – 68

Gilson HPLC Systems 

Gilson high performance liquid chromatography (HPLC) systems 

provide complete solutions for all purification needs. Front-end 

liquid handlers and autosamplers are available in a range of bed 

capacities and optimized bench space footprints. Several models 

also accommodate innovative injection and fraction collection 

capabilities within a single platform. Trilution® LC Software 

completely controls the entire HPLC system, allowing focus to 

remain on the science rather than the software. 

Gilson’s VALVEMATE® II Valve Actuator offers multiple column 

selection availability featuring: fast switching times, high 

pressure valve control of 6 or 10-port, 2, 6 or 10-position and 

low pressure valve control of 6 or 8-positions. 

(see page 47) 

The newest innovation from 

Gilson, the GX-271 Liquid 

Handler, is a mid-size capacity 

and throughput instrument 

fulfilling numerous laboratory 

needs at an economical price. 

(see pages 7, 8, 12 & 13) 

The 215 Series Liquid 

Handlers, known as the 

workhorses of the industry, 

are available in single, 

multiple and variable 

spanning probe models. 

(see page 14) 

Gilson’s accurate pumping solutions are available 

with multi-solvent selection, high pressure variable 

mixing and interchangeable pump heads. 

(see pages 15 – 19) 

The GX-281 Liquid Handler 

is Gilson’s premier front-end 

instrument featuring a large 

bed capacity and a wide 

array of components such 

as automatic reinjection of 

fractions using innovative 

GX Z-Injection technology. 

(see pages 5, 6, & 11) 

Gilson’s variety of detection 

solutions offers excellent 

sensitivity and reproducibility 

including diode array (DAD), 

ultraviolet (UV) and/or visible 

(VIS) and evaporative light 

scattering (ELS) detection. 


Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Systems.

HPLC: Systems 

Column ID 

2 mm 

4.6 mm 

10 mm 

20 mm 

30 mm 

50 mm 

100 mm 

Description 

Page 

Number 

The GX-281 Preparative HPLC System allows for 

injection, fraction collection and reinjection. 

The system is also ideal for liquid handling 

techniques such as sample mixing, dilutions 

and sample extract transfers. 

Sample Loading Capacity 

15 g/injection 

Injection Loop Volume 

250 µL – 25 mL 

Specifications 

Optimal Column Size 

4.6 – 100 mm 

Flow Rate 

1 – 200 mL/min 

Maximum Injection 

Pressure 

3,000 psi (207 bar) 

Maximum Pump Pressure 

3,040 psi (210 bar) 

GX-281 

Preparative 

HPLC System 

GX-281 

Analytical to 

Semi-preparative 

HPLC System 

GX-271 

Preparative 

HPLC System 

GX-271 

Analytical to 


HPLC System 

flow rate 1 – 200 mL/min 

injection loop volume 250 µL – 25 mL 

sample mass 15 g/injection 

flow rate 0.15 – 30 mL/min 


sample mass 300 mg/injection 




flow rate 0.15 – 30 mL/min 


sample mass 300 mg/injection 

5 

6 

7 

8 

The GX Z-Injection Module 

allows sample aspiration 

directly into the valve 

without a separate injection 

port, eliminating a source of 

potential carryover. 

GX Direct Injection technology 

features an injection port that 

connects directly to the valve, 

minimizing volume associated 

with connective tubing. 

(located behind the racks) 

The prep-scale HPLC pumps 

feature low-dwell volumes 

which allow for fast, 

reproducible gradients. 

(see pages 15-19) 

845Zprep 


to Preparative 

HPLC System 

cLC Analytical 

to Preparative 

HPLC System 







flash chromatography 

9 

9 

The GX Prep Solvent 

System features a 

dynamic range of 

volumes and flow 

rates using syringeless 

technology within a 

solvent delivery pump 

capable of accessing 

five off-bed solvents. 


HPLC: Systems 

The GX-281 is a multi-functional instrument that can 

perform injection, fraction collection and reinjection. 


300 mg/injection 



2 µL – 2 mL 

A single, mid-size instrument for injection and fraction 

collection with separate fluid pathways to reduce 

cross-contamination of fractions. 





250 µL – 25 mL 

The GX-281 features a Jet Wash 

Rinse station design that delivers 

individual streams of solvent 

for each separate rinse station 

to actively wash the outside of 

probes. Four rinse solvents are 

available for selection. 

The GX Z-Injection Module allows 

fast injections with low carryover 

and low maintenance by aspirating 

the sample directly into the valve. 


2 – 20 mm 

Maximum Injection Pressure 

5,000 psi (345 bar) 

Separate injection and fraction collection 

paths reduce cross-contamination of 

fractions from sample injection. The cycle 

time of the injection is minimized by 

eliminating rinse steps which are required 

with single fluid path configurations. 

Flow Rate 

H1: 150 µL/min – 15 mL/min 



H1: 8,700 psi (600 bar) 

H2: 4,350 psi (300 bar) 


HPLC pumps are 

offered to perform 

binary gradients. 


The GX Prep Solvent System 

uses syringeless technology 

and allows access to five 

off-bed solvents. 


10 – 100 mm 


3,000 psi (207 bar) 

The GX Direct Injection 

Module can utilize both 

1/16” and 1/8” injection 

valves, allowing for a wide 

variety of sample volumes 

and loop options. 

A VALVEMATE® II 

Valve Actuator can 

be incorporated for 

multiple column 

selection or column 

regeneration 

applications. 

(see page 47) 

Flow Rate 

1 – 200 mL/min 


3,040 psi (210 bar) 

Binary gradient preparative 

HPLC pumps provide 

high-flow capabilities. 


Detection solutions are available for 

DAD, fixed or variable wavelength 

UV/VIS, UV and ELS detection. 


Mid-to-large collection 

capacity in a small footprint. 

Modular detector options available. 



HPLC: Systems 

The GX-271 is a mid-size liquid handler/fraction collector with 

a bench saving footprint featuring separate fluid pathways that 

reduce cross-contamination of samples and fractions. 


300 mg/injection 


2 – 20 mm 


5,000 psi (345 bar) 



2 µL – 5 mL 

Flow Rate 


H2: 30µL/min – 30 mL/min 


H1: 8,700 psi (600 bar) 

H2: 4,350 psi (300 bar) 

Gilson’s 845Zprep System features Gilson’s first generation Z-Injection technology 

accommodating volumes and flow rates for semi-preparative to preparative applications. 

Mid-to-large bed capacity, 

accommodating both 

samples and fractions. 





3,000 psi (207 bar) 


4.6 – 50 mm 


3,040 psi (210 bar) 

The GX Analytical Solvent System features 

innovative syringeless technology and 

access to five off-bed solvents. 

Binary gradients are 

achieved with analytical 

to semi-preparative 

HPLC pumps. 


For confirmation or added 

detection capabilities of nonchromaphoric 

compounds 

configure an additional ELS 

detector with a multi-channel 

DAD detector. 



100 μL – 10 mL 

Injection Variability 

< 1.5% 

Carryover 

< 0.01% 

Flow Rate 

1 – 200 mL/min 

Gilson’s cLC System is a complete chromatography system capable of automating the purification of compounds using both 

normal and reversed-phase columns. The cLC System has the ability to accommodate various modes of detection including 

DAD, UV/VIS, ELS and RI. Additional analytical components are also integrated onto the cLC System enabling automated on-line 

evaluation of the collected fractions for purity and recovery. 


The low mount collection valve enables fraction 

collection to tubes and contains two separate 

liquid paths which reduce cross-contamination. 




4 – 100 mm 


5,000 psi (345 bar) 


5 µL – 25 mL 

Flow Rate 

1 – 200 mL/min 


33X Pump: 3,040 psi 

32X Pump: 8,700 psi 


HPLC: Liquid Handlers/Injectors 

GX-281 Liquid 

Handler/ Injector 

GX-271 

Preparative Liquid 

Handler/Injector 

GX-271 

Analytical Liquid 


215 Liquid 


Multiple 

Probe 215 Liquid 


Liquid 

Handler 

Fraction 

Collection 

Injection 

Volume 

Liquid Handling 

Accuracy 

Injection 

Carryover 

Gilson’s liquid handlers and injectors offer the 

broadest range of instrument capabilities on the 

market today. From small footprint injectors to 

larger footprint liquid handler/injector/fraction 

collectors, Gilson provides the ultimate front-end 

automation solutions for every HPLC system. 

Page 

Number 

250 µL – 25 mL 98% (100 µL – 25 mL) < 0.005% 11 

250 µL – 25 mL 98% (50 µL – 25 mL) < 0.05% 12 

2 µL – 2 mL 98% (10 µL – 25 mL) < 0.05% 13 

5 µL – 10 mL 99.7% (50 µL) < 0.1% 14 

5 µL – 5 mL 99.2% (500 µL) < 0.05% 14 

Specifically engineered for semi-preparative to 

preparative HPLC requirements, the GX-281 offers 

unmatched versatility and performance for front-end 

LC automation including revolutionary syringeless 

pumping technology. 

GX-281 Liquid Handler/Injector 

Bed Capacity 

Tube 

Diameter 

Immediate response 

aspiration and 

dispensing with the 

GX Prep Solvent 

System eliminates 

time spent waiting for 

valves to switch and 

syringes to fill. 

Sample 

Quantity 

13 mm 576 

15 mm 576 

16 mm 450 

18 mm 420 

25 mm 180 

28 mm 162 

Note: tubes up to 220 mm 

tall can be accommodated 

(see page 51 for the Gilson Rack/Instrument 

Compatibility Chart) 

% Mobile Phase 

mVolts 

Two unique and separate 

liquid pathways for 

injection and fraction 

collection provide a zerocontamination 

environment. 

Reproducibility 

< 1% CV (total loop) 

< 3.5% (partial loop) 

Accuracy 

98% (100 µL – 25 mL) 


250 µL – 25 mL 


Injection Carryover 

< 0.005% 

Injection, collection and analysis 

Fraction Zone, 1 










Time (minutes) 


Module features an 

injection port that 

connects directly to 

the injection valve for 

decreased dead volume 

and reduced maintenance. 

Flow Rate 

1 – 200 mL/min 


5,000 psi (345 bar) 

Dimensions (w x d x h) 

87.5 x 76.2 x 96.5 cm (35 x 30 x 38 in) 



Product #s: 261030 (GX-281 Liquid Handler) 

261031 (GX-281 Liquid Handler with bar code) 

261355 (GX Direct Injection Module, 1/8", prep) 

261354 (GX Direct Injection Module, 1/16", prep) 

261356 (GX Direct Injection Module,1/16", analytical SS) 

261357 (GX Direct Injection Module, 1/16", analytical PEEK) 

261353 (GX Z-Injection Module) 

Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Liquid Handlers/Injectors. 11


Flow Rate 

1 µL/min – 50 mL/min 

Accuracy 

98% (50 µL – 25 mL) 


GX-271 Preparative Liquid Handler/Injector 


250 µL – 25 mL 


< 0.05% 




5,000 psi (345 bar) 


59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in) 

The GX-271 Liquid Handler/Injector is the perfect 

blend of size, performance and capacity, consuming 

only 23" of linear bench space. Large-bore injection 

modules feature a continuous flow path design 

for preparative applications. Small-bore valves 

are available for analytical to semi-preparative 

applications with inert injection modules for biocompatible 

applications. 

The GX-271 Liquid Handler/Injector can be 

configured for large injection loop volumes with 

minimal sample waste and carryover as well as 

to accommodate either the GX Prep or Analytical 

Solvent System which feature syringeless 

pumping technology. 

Flow Rate 

1 µL/min – 5 mL/min 

Accuracy 

98% (10 µL – 25 mL) 


GX-271 Analytical Liquid Handler/Injector 


2 µL – 2 mL 


< 0.05% 



< 3.5% (partial loop) 


5,000 psi (345 bar) 


59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in) 


100 

80 

60 

40 

20 

0 

mVolts 

300 

250 

200 

150 

100 

50 

0 

Recovery data: Theophylline (5 mg/mL), Caffeine (5 mg/mL) and 

Phenol (3 mg/mL) solution with 1 mL injection loop volumes 

calculated from reinjection of collected Theophyline fractions (Zone 2) 




-50 

0 1 2 3 4 5 

Ethernet based communication 

provides better connectivity. 

Jet Wash Rinse technology provides 

flowing rinse for fast and efficient 

rinsing protocols utilizing up to two 

separate flowing rinse solvents. 


Module increases performance 

by incorporating a port that 

connects directly to the 

injection valve, reducing sample 

waste and maintenance. 


100 

80 

60 

40 

20 

0 

mVolts 

Series of ten 20 µL total loop injections of Theophylline, Caffeine and Phenol 

1250 

1000 

Phenol 

Theophylline 

750 

500 

Caffeine 

250 

0 

-250 

0 2 4 6 8 10 12 



Injection 

Recovery 

1 99.86% 

2 96.61% 

3 98.22% 

4 96.64% 

5 100.10% 

6 98.99% 

7 99.57% 

8 99.69% 

9 99.81% 

10 100.36% 

Average Recovery 99.99% 

Standard Deviation 0.01 

CV 1.39% 

Available in an analytical or prep model, 

the solvent systems feature dynamic 

flow rate ranges and can access five 


Peak 

Retention 


Area Count 

(µVmin/100) 

Standard 

Deviation 

Theophylline 2.8 14901136.11 44928.68 0.30% 

Caffeine 4.2 12267689.70 36503.01 0.30% 

Phenol 8.4 13314605.43 73087.21 0.55% 

Separate fraction collection flow paths prevent 

contamination from the injection process. 

Product #s: 2614107 (GX-271 Preparative Liquid Handler) 

2614108 (GX-271 Analytical Liquid Handler) 

2614101 (GX-271 Liquid Handler without pump) 

261355 (GX Direct Injection Module, 1/8”, prep) 


261356 (GX Direct Injection Module,1/16”, analytical SS) 

261357 (GX Direct Injection Module, 1/16”, analytical PEEK) 

CV 

GX-271 Preparative 

and Analytical Liquid 


Bed Capacity 

Tube 

Diameter 

Sample 

Quantity 

10 mm 540 

13 mm 300 

15 mm 220 

16 mm 220 

17 mm 220 

18 mm 220 

28 mm 70 

Note: tubes up to 

200 mm tall can be 

accommodated 



Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Liquid Handlers/Injectors. 13


HPLC: Pumps 

This versatile, large bed liquid 

handler accommodates more 

than 3,000 racks to hold virtually 

any sample vessel. 

Customize by adding 

accessories and 

components to meet 

laboratory needs. 

The 215 Liquid Handler/Injector, a true and trusted 

laboratory workhorse for many years, performs 

injection, fraction collection and reinjection of collected 

fractions within a single platform. 

Flow Rate 

Pressure 

1 mm 

Column ID 

2 mm 

4 mm 

10 mm 

20 mm 

> 50 mm 

Page 

Number 

Product #s: 2510121 (215 Liquid Handler) 

251511 (819 Injection Module) 

251551 (845Z Injection Module) 

251552 (845Zprep Injection Module) 

333/334 

Prep-scale 

HPLC Pumps 

1 – 200 mL/min 

3,040 psi 

(210 bar) 

16 

Accuracy 

liquid handling: 99.7% (50 μL) 


5 μL – 10 mL 



liquid handling: 1.37% CV (50 μL) 

injection: < 0.9% (1 mL) (total loop) 


< 0.1% 


91 x 61 x 56 cm (36 x 24 x 22 in) 

331/332 


HPLC Pumps 

321/322 

Analytical to 


HPLC Pumps 

0.5 – 50 mL/min 

H1: 0.15 - 15 mL/min 

H2: 0.3 - 30 mL/min 

8,700 psi 

(600 bar) 

H1: 8,700 psi 

(600 bar) 

H2: 4,300 psi 

(300 bar) 

17 

17 

30X Series 

HPLC Pumps 

10 µL/min – 

200 mL/min 

8,700 psi 

(600 bar) 

18 

Inject onto FIA/MS, parallel HPLC 

and MUX systems using the optional 

849 Multiple Injection Module. 

The Multiple Probe 215 Liquid Handler/Injector is capable 

of high-throughput automation of all basic liquid handling 

tasks such as sample transfer, dilutions, mixing, reagent 

addition and plate reformatting. 

402 

Syringe 

Pumps 

Minipuls 

Evolution® 

Peristaltic 

Pump 

1 µL/min – 

120 mL/min 

0.2 µL/min – 

340 mL/min 

110 psi (8 bar) 

for 0.1 – 10 mL syringes 

40 psi (3 bar) 

for 25 mL syringe 

42 psi 

(3 bar) 

— 18 

— 19 

Product #: 25101311 (Multiple Probe 215 Liquid Handler) 

Configure with the 0.015" ID 

analytical valve or the 0.040" 

ID preparative valve. 

Product #: 2515154 (849 Multiple Injection Module) 

The 849 Multiple Injection Module allows 

four simultaneous injections, ideal for 

parallel HPLC and MUX systems. 



liquid handling: 0.57% CV (500 μL) 

injection: < 5% CV (500 μL) (partial loop) 

method: < 2% CV (500 μL) (total loop) 

Accuracy 

liquid handling: 99.2% (500 μL) 


5 μL – 5 mL 

Valve Switching Speed 

RV700-100: < 135 ms 

RV700-112: < 370 ms 


< 0.05% 


97.8 x 61 x 55.8 cm (38.5 x 24 x 22 in) 

Multiple Injection Module Option 


61 x 18 x 13 cm (24 x 7 x 5 in) 

Minipuls 3 

Peristaltic 

Pump 

standard 

(1, 2, 4 or 8 channels): 

0.3 µL/min – 30 mL/min 

high-flow rate 

(2 or 4 channels): 

1 – 220 mL/min 

standard: 


high-flow rate: 


— 19 

Gilson offers unsurpassed flexibility in HPLC 

pumping systems with an extensive product 

range and accommodations. 

Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Pumps. 

15

HPLC: Pumps 

Capable of fast separations with high-efficiency columns in normal 

and reversed-phase modes, Gilson’s 333 and 334 Pumps are the 

premier solvent delivery solutions for preparative chromatography. 

Choose the 333 master pump for isocratic applications or the 

333/334 binary system for composition gradient with high 

pressure mixing (third pump for ternary). 

Stackable to conserve 

bench space. 

Flow Rate 

1 – 200 mL/min (3,040 psi) 



< 0.5% or 2.7 μL/min RSD 

Accuracy 

± 1% or 5.3 μL/min 


333: 26 x 41 x 51 cm (10 x 16 x 20 in) 

334: 26 x 41 x 39 cm (10 x 16 x 15 in) 

Gilson’s 331 and 332 dual-piston pumps for semi-preparative 

and preparative chromatography feature a variable volume, 

dynamic mixer for optimized gradient performance. 

Trilution® LC Software provides control 

within a complete semi-preparative system 

including data processing and result 

reporting or stand-alone control via the 

keypad incorporated on the 331 Pump. 

Smooth linear transposition from analytical 

to preparative HPLC methods using 4 to 

20 mm ID columns packed with highefficiency 

stationary phases. 

Milligram-level sample mass-throughput per 

injection; 150 mg with a 20 mm bore column. 

Solvent selection on each pump 

with optional 4-solvent valve for 

up to eight solvents. 

Programmed Composition (% E) 

mVolts 

120 

100 

80 

60 

40 

20 

Gradient Linearity and Accuracy 

0 

0 

0 5 10 15 20 25 30 


210 

180 

150 

120 

90 

60 

Capable of milligram-to-gram 

level mass-throughput 

per injection up to 15 

depending on column size 

and load capacity. 

Selection of up to four 

solvents per pump for a total 

of eight solvents in a binary 

pumping system. 

Retention Time Repeatability 

300 

250 

200 

150 

100 

30 

20 

0 

-30 

0 2 4 6 

0 

8 


Series of Ten Injections 

50 

100 

80 

60 

40 

Detected Composition (mAU) 


Methyl PB Ethyl PB Propyl PB 

Average Retention 

Time 

3.44 4.85 6.08 

Standard 

Deviation 

0.02 0.02 0.02 

CV 0.72% 0.34% 0.29% 

The entire preparative HPLC 

system is controlled with Trilution® 

LC Software, additionally the 

333 model has an incorporated 

keypad for stand-alone operation. 

The flow rate 

can be doubled 

with additional 

parallel pumps 

to 400 mL/min. 

Product #s: 38103331 (left) (333 Pump, H3 primary solvent, dual-piston, reciprocating master pump) 

38103341 (right) (334 Pump, H3 secondary solvent, dual-piston, reciprocating remote controlled pump) 

Product #s: 38103312 (right) (331 Pump, H2 primary solvent, dual-piston, reciprocating master pump) 

38103322 (left) (332 Pump, H2 secondary solvent, dual-piston, reciprocating remote controlled pump) 

The 321 and 322 multi-solvent pumps are designed for use with Gilson’s 

HPLC analytical and semi-preparative chromatography systems. 

Product #s: 38103211 (321 Pump, H1) 

381032211 (left) (322 Pump, H1) 

38103212 (right) (321 Pump, H2) 

381032221 (322 Pump, H2) 

H1 pump heads are ideal 

for 2 to 10 mm ID columns. 

H2 pump heads 

are ideal for 

4 to 20 mm 

ID columns. 

Flow Rate 

0.5 – 50 mL/min (8,700 psi) 



H2: ± 0.7% or 7 μL/min 

Accuracy 

± 2% or 13 μL/min 


331: 26 x 41 x 51 cm (10 x 16 x 20 in) 

332: 26 x 41 x 39 cm (10 x 16 x 15 in) 

High pressure Adjustable Volume Dynamic Mixer (AVDM) 

enables fast gradient response times and homogeneous 

mixtures for analytical to semi-preparative applications. 

While the pumps are controlled via Trilution® LC, the 321 also 

incorporates a keypad that allows independent stand-alone 

gradient control. 

A single 321 or 322 Pump is capable of binary gradients. 

Flow Rate 

H1: 0.15 – 15 mL/min (8,700 psi) 

H2: 0.3 – 30 mL/min (4,300 psi) 



H1: < 0.7% or 2 µL/min 

H2: 4 µL/min 

Accuracy 

± 2% or 13 μL/min 


321: 26 x 41 x 51 cm (10 x 16 x 20 in) 

322: 26 x 41 x 39 cm (10 x 16 x 15 in) 


17

HPLC: Pumps 

Together with Gilson’s manometric modules and dynamic mixers, the 

305, 306 and 307 Pumps offer a cost-effective, space saving and robust 

solution for isocratic or gradients of analytical to preparative HPLC. 

The 307 model functions as an isocratic pump with a built-in manometric module. 

Dynamic mixers ensure efficient, dependable gradient creation. 

The 306 model functions as a 

slave unit, controlled by either a 

305 or Trilution® LC Software. 

The 305 and 307 Pumps are 

programmable through the 

incorporated keypad. 

Tools are not required for 

pump head removal, allowing 

accessibility for maintenance. 

Piston seals, check valves and 

other parts can be replaced in 

a matter of minutes. 

Offering unmatched flexibility and performance for liquid transfer applications, the Minipuls Evolution® is designed 

for scientists seeking a high performance, manageable peristaltic pump capable of a variety of configurations. 

Gilson offers the only peristaltic pump that allows up to 16 separate 

channel cartridges to be changed while the pump is running with a 

simple push on the cartridge for placement and removal. 


≤ 0.1% on all channels 


Tubing ID 

standard: 0.25 – 4 mm 

high-flow: 2 – 8 mm 


Optimized six roller drive 

limits pulsation. 

Flow Rate 

standard: 0.2 µL/min – 70 mL/min (42 psi, 3 bar) 

high-flow: 1 – 340 mL/min (29 psi, 2 bar) 

Flow Rate 

305/306: 10 μL/min – 200 mL/min 

307: 10 μL/min – 10 mL/min 


< 1% 


33 x 33 x 15 cm (13 x 13 x 6 in) 

Accuracy 

< ± 1% 

Product #s: 360261 (305 Pump) 

3602661 (right) (306 Pump) 

3602671 (left) (307 Pump) 

Product #: F110701 

(Minipuls Evolution® Pump) 

Head Speed 

continuous adjustment from 0 – 60 rpm by 0.01 rpm increments 

up to 9.99 rpm, 0.1 rpm increments above 10 rpm 


Module: 164 x 182 x 169 mm 

MF1 pump head: 164 x 56 x 169 mm 

MF4 pump head: 164 x 92 x 169 mm 

HF2 pump head: 164 x 92 x 169 mm 

The 402 Syringe Pumps assure accuracy in sample transfers, 

dilutions, reagent additions, mixing and more. They offer 

speed and reliability for repetitive liquid handling tasks. 

Available in three configurations: 

402 Single, ideal for 

sampling injectors. 

402 Dual with Tee, provides 

rapid dilutions in both 

small and large volumes. 

An ideal addition to Gilson liquid handlers and injectors 

including the GX and 215 Series instruments. 

402 Dual, two-in-one accommodates 

simultaneous dispensing of multiple 

reagents and solvents. 

The Gilson Minipuls 3 multi-channel peristaltic pump is simple to integrate onto existing systems 

with control through an incorporated keypad or remotely with a separate device. 

Enhanced reliability and ease of 

operation are possible because 

the pump’s speed stability is 

maintained independent of 

normal temperature variations 

from 0˚ to 40˚C. This makes 

the pump ideal for biological 

perfusion applications. 

(Note: not intended for direct 

connection to human subjects.) 

The Minipuls 3 accommodates wide flow rate ranges using 

either the standard or high-flow pump head. Pump heads 

are available with one, two, four or eight channels. 

Multiple stainless steel rollers drive the pump to 

produce smoother, low pulse flows. 


Product #s: F410511 (left) (402 Single Syringe Pump) 

F410512 (right) (402 Dual with Tee Syringe Pump) 

F410513 (402 Dual Syringe Pump) 


0.8% (10 μL) 

Accuracy 

98.2% (10 μL) 


Flow Rate 

1 μL/min – 120 mL/min 


17 x 20 x 24 cm (6.7 x 7.9 x 9.4 in) 

Product #: F155004 (Minipuls 3 Pump, single channel) 

Flow Rate 

standard: 0.3 μL/min – 30 mL/min 

(72 psi, 5 bar) 

high-flow: 1 – 220 mL/min 

(42 psi, 3 bar) 

Tubing ID 

standard: 0.25 – 4 mm 

high-flow: 2 – 8 mm 

Head Speed 

continuous adjustment from 0 – 48 rpm 

by 0.01 rpm increments up to 9.99 rpm, 

0.1 rpm increments above 10 rpm 


15 x 17.5 x 18 cm (5.9 x 6.9 x 7.1 in) 


19

HPLC: Detectors 

171/172 

Diode Array 

Detectors 

15X Series 

UV/VIS 

Detectors 

112 UV 

Detector 

Diode Array 

(DAD) 

Ultraviolet 

(UV) 

Visible 

(VIS) 

Evaporative 

Light Scattering 

(ELS) 

Wavelength 

190 - 720 or 

190 - 610 nm 

190 - 700 nm 9 nm 

254 or 

280 nm 

Spectral 

Band Width Channels Page 

Number 

— 8 21 

0.2 or 

12 nm 

dual & 

single 

21 

single 22 

Gilson’s recently released 171 and 172 Diode Array Detectors deliver 

rugged and accurate detection for both preparative and analytical 

HPLC. Detection is done with a fiber optic light path that can be 

configured in a fixed or remote flow cell design. 

Product #s: 18100001 (171 Diode Array Detector) 

18100002 (172 Diode Array Detector) 

With remote flow cell, the power of the detector is located directly at 

the outlet of the column, which yields virtually no peak broadening. 

An interchangeable flow cell design allows for both the 171 and 

172 Detectors to accommodate analytical and semi-preparative to 

preparative applications. 

Pixels 

171: 256 

172: 512 

Wavelength 

171: 190 - 720 nm 

172: 190 - 610 nm 

Wavelength Accuracy 

< 0.5 nm 

Baseline Drift 

5 x 10 -04 AU/h 


S/N Ratio 

171: < 3 x 10 -04 AU 

172: < 2 x 10 -04 AU 

Pixel Distance 

171: ~2.2 nm/pixel 

172: ~0.8 nm/pixel 

Wavelength Repeatability 

< 0.1 nm 

Wavelength Temperature Drift 

< 0.005 nm/°C 


26.5 x 43.5 x 15.6 cm (10.4 x 17.1 x 6.2 in) 

prepELS II 

Detector 

— — single 22 

Gilson offers a complete line of HPLC detection solutions 

that are ideal for analytical, semi-preparative and 

preparative HPLC applications. 

Gilson’s 151, 152, 155 and 156 UV/VIS Detectors deliver versatile, 

rugged detection with an interchangeable flow cell design to 

accommodate analytical to preparative HPLC applications. 

(see page 59 for Flow Cell Selection Chart) 

The following modes are available to specify detection 

conditions and basic operating parameters: 

– Single wavelength with dual sensitivities (151 and 152) 

– Dual wavelengths (155 and 156) 

– Scan wavelengths (155 and 156) 

Choose either Gilson’s Trilution® LC Software or the standalone 

keypad control on the 151 and 155 Detectors. 

Up to ten methods can be stored. 

120 

100 

mVolts 

100 

80 

60 

40 

20 

0 

-20 

0 2 4 6 


80 

60 

40 

20 

0 

% Moblie Phase 

Product #s: 10105311 (right) (151 UV/VIS Detector, with flow cell/accessory kit) 

10105411 (152 UV/VIS Detector, with flow cell/accessory kit) 

10105511 (155 UV/VIS Detector, with flow cell/accessory kit) 

10105611 (left) (156 UV/VIS Detector, with flow cell/accessory kit) 

Lamp 

UV: deuterium, 750 hrs 

VIS: tungsten/halogen, 500 hrs 

Wavelength 

190 – 700 nm 

Drift 

3 x 10 – 4 AU/hr 

Sensitivity 

0.001 – 2 AU 

Spectral Band Width 

9 nm 


Setting Accuracy 

± 2 nm 

Setting Precision 

± 0.2 nm 


26.5 x 43.5 x 15.6 cm (10.4 x 17.1 x 6.2 in) 

Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Detectors. 

21

HPLC: Detectors 

The 112 UV Detector is a rugged, 

economical detector for analytical or 

preparative HPLC and GPC clean-up. 

A front panel dial 

allows selection of 

sensitivity level to 

adjust the absorbency 

of a sample. 

Accessible lamp/filter assemblies for selecting wavelengths. 

Fixed wavelength design for increased sensitivity and durability. 

10 mm pathlength analytical flow cell provides excellent sensitivity ranging 

from 0.001 to 1 AUFS (Absorbance Units, Full Scale). 

Reduced operating and setup time with a single step baseline function. 

Event-marking function conveniently marks injection point or fraction collector 

advance on either the recorder or data system. 

Large 

Capacity 

HPLC: Fraction Collectors 

Small 

Flow Rate Microplate Tubes 1 L Bottles 

Page 

Number 


prepFC 

Fraction 

Collector 

200 mL/min 24 

Product #: 0310511 

(112 UV Detector, with lamp and 11 μL flow cell/accessory kit) 

Lamp 

mercury (standard): 254 nm, 2,000 hrs 

phosphor-coated mercury: 280 nm, 500 hrs 

Spectral Band Width 

254 nm: 0.2 

280 nm: 12 

Drift 

3 x 10 – 4 AU/hr 

Wavelength 

254 or 280 nm 

Flow Cell Sensitivity 

< 4 x 10 – 4 AU for flow step 

of 0.5 – 2 mL/min (Methanol) 


32 x 32 x 13 cm (12.6 x 12.6 x 5.2 in) 

FC 204 

Fraction 

Collector 

FC 203B 

Fraction 

Collector 

20 or 200 

mL/min 

20 mL/min 25 

25 

Gilson’s evaporative light scattering (ELS) detector, prepELS II, provides 

capabilities on the analytical-scale with the ability to detect as low as 

nanograms of analyte while a unique filtering technology removes 

baseline noise without distressing peak height or shape. 

mVolts 

150 

125 

100 

75 

50 

25 

ELSD 

UV 

0 

0 

0 2 4 6 


100 

80 

60 

40 

20 

% Moblie Phase 

The prepELS II Detector is ideal for obtaining 

the purest fractions with the ability to detect 

virtually any analytes with or without a 

chromophore, including compounds that 

have poor UV absorption such as sugars, 

carbohydrates, lipids and triglycerides. 

Gilson offers advanced solutions for fraction 

collection with a complete line of stand-alone and 

software-controlled fraction collectors ranging from 

small footprint to large vessel capacity models and 

everything in between. 


Light Source 

670 nm laser diode, < 5 mW 

Gas Requirement 

65 ± 5 psi Nitrogen or Argon 

Nebulization Temperature 

0˚ – 70˚C 

Evaporative Zone Temperature 

ambient – 120˚C 

Liquid Flow Rate 

0.2 – 5 mL/min 

Splitting packages available to accommodate 

system flow rates up to 75 mL/min 

Dimensions 

25 x 46 x 29 cm (9.8 x 18 x 11.5 in) 

Product #s: 

18100100 (prepELS II Detector, 120V) 

18100200 (prepELS II Detector, 240V) 

Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Fraction Collectors. 

23

HPLC: Fraction Collectors 

Gilson’s prepFC Fraction Collector is a large capacity, compact 

footprint fraction collector ideal for semi-preparative to preparative 

HPLC applications. The prepFC features an adjustable tray that 

accommodates various vessel heights, volumes and sizes. 

Sample 

Quantity 

56 300.0 (20 mL) scintillation vials (4) Code 333 Racks 

250.0 

200.0 

432 10 x 75 mm (4 mL) tubes (4) Code 341 Racks 

150v 

mVolts 

100.0 

320 13 x 100mm (9 mL) tubes (4) Code 343 Racks 

50.0 

0.0 

0.0 1.0 2.0 

Minutes 

prepFC Bed Capacity 

Size Vessel Rack 





54 28 x 57 mm (25 mL) scintillation vials (2) Code 204 Racks 

54 30 x 115 mm (50 mL) conical bottom 

tubes 

(2) Code 222 Racks 

18 48 x 113 mm (125 mL) bottles (2) Code 211 Racks 

11 (500 mL) bottles (1) Code 91 Rack 

81 (1 L) bottle (1) Code 92 Rack 

240 12 x 32 mm (2 mL) vials (4) Code 330 Racks 


(see page 51 for the Gilson Rack/Instrument Compatibility Chart) 


50.3 x 39.1 x 34.8 cm (19.8 x 15.4 x 13.7 in) 

Liquid Contact Material 

PTFE and 316 stainless steel 


< 20 ms 


prepFC Control Software: 

• Remote start and tube advance 

• Up to ten programmable collection windows 

• Active chromatogram display with start and 

stop fraction collection lines 

• Customizable collection patterns and 

rack addition 

Increased X/Y speed optimizes 

fraction collection. 

Valve Dead Volume 

114 μL internal volume; 6 μL dead volume 

from common port to normally closed port 

Flow Rate 

200 mL/min 

Collection Modes 

slope, level, time or volume 

High-flow fraction collection valve. 

Choose the compact FC 203B Fraction Collector 

for small capacity collection or the FC 204 for 

larger volumes. Collect fractions by time, drop 

or peak, accommodating all LC applications. 

Both models accept a wide variety of collection 

vessels including tubes, vials and microplates. 

The 3-way diverter valve prevents contamination of collected 

fractions (standard on FC 204 and optional on FC 203B). 

Up to ten programmable time windows can be 

added for collecting only samples of interest, 

while discarding the column’s void volume, areas 

of non-interest and equilibration volumes. 



FC 203B: 32.4 x 29.2 x 26.7 cm (12.8 x 11.5 x 10.5 in) 

FC 204: 47.9 x 46.4 x 33 cm (18.9 x 18.3 x 13 in) 

Liquid Contact Material 

PTFE and 316 stainless steel 

In peak mode, collectors monitor a detector signal 

and identify peaks using either an adaptive-slope 

algorithm or a specified mV threshold. 

The fraction collector utilizes multi-column adapters 

for collecting up to 18 separate channels. 

Its compact size fits in most fume hoods. 


100 ms 

Flow Rate 

FC 203B: 20 mL/min 

FC 204: 20 or 200 mL/min 

Valve Dead Volume 

FC 203B/FC 204: 64 μL internal volume; 3.5 μL dead volume from common port to normally closed port 

FC 204: 114 μL internal volume; 6 μL dead volume from common port to normally closed port 

Contact closure inputs are available 

to remotely start, advance or stop 


Pocket PC control 

for stand-alone 

operation allows the 

prepFC to be added 

to any HPLC system 

capable of sending 

contact closures. 

Increase bed capacity 

by configuring up 

to four fraction 

collectors in a single 

system (provides 

over 1,000 samples). 

Product #s: 17110101 (prepFC Fraction Collector - Ethernet) 

171071 (prepFC Fraction Collector) 

Product #s: 171011 (right) (FC 203B) 

171011DV (FC 203B, 3-way valve) 

171041 (left) (FC 204) 

171043 (FC 204, prep valve) 

Stand-alone instruments are 

controlled with an incorporated 

keypad or through Gilson’s 

Trilution® LC Software. 

Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Fraction Collectors. 

25

Control Software 

Gilson has redefined purification with the HPLC system control software, Trilution® LC. 

This liquid chromatography software, specifically designed for purification, allows for 

complete control to achieve the challenging results that applications require. 

HPLC Systems 

SPE Systems 

GPC Clean-up 

Systems 

Liquid Handlers 

Page 

Number 

Trilution® LC 27 

Trilution® LH 28 

Configuration and Virtual Lab Screen 

Gilson offers powerful software to complement 

robust instruments. With accommodating features, 

drag-and-drop functionality and numerous supplied 

tasks, Gilson’s software packages allow effortless yet 

complete control of instruments focusing on the 

science rather than the software. 

Intel, Pentium and Core are trademarks of Intel Corporation or its subsidiaries in the United States and 

other countries. Microsoft and Windows are registered trademarks of the Microsoft Corporation in the 

United States and/or other countries. 

Application Run Screen 

Fraction Collection Capabilities: 

• Collection modes: slope, level, time or volume 

• Conditional logic fraction collection 

• Graphical fraction collection optimization 

• Slope collection settings: front, tail and APEX 

• Auto sample list generation 

Open Access Module: 

• Administrator control and permissionsetting 

capabilities 

• Walk-up user access 

• Minimal training required 

Conforms to the application needs rather than compromising 

the application in order to fit the software. 

Method Builder Control Screen 

Computer Recommendation 

Processor 

Intel® Pentium® 4 

(2.8 GHz); 

Intel® Core 2 Duo 

(1.86 GHz) 

RAM 

2 GB 

Fraction Collection Settings Page 

Product #s: 210631R20 (Trilution® LC Media) 

21063130 (Trilution® LC Lifetime License) 

21063130L5 (Trilution® LC Multi-user Lifetime License - 5) 

21063130L10 (Trilution® LC Multi-user Lifetime License - 10) 

21063131 (Trilution® LC 1 year License) 

Note: Requires Oracle® Database 10g Express Edition 

Fixed Drive 

120 GB, available 

space: 4 GB 

(6 GB at install) 

Additional Drive 

DVD/CD-RW 

Video Card 

1280 x 1024 with 

24 bit True Color 

Serial Port 

1 

Operating System 

Microsoft® Windows® XP Professional SP2 or later 

Microsoft® Windows® 2000 SP4 

Visit www.gilson.com or call 608-836-1551 for more details on Gilson Control Software. 

27

Control Software 

Trilution® LH Software, designed by scientists for scientists, is Gilson’s complete control 

solution for liquid handling and solid phase extraction applications. Trilution LH 

features a user-friendly graphical interface with icon-based drag-and-drop functionality 

minimizing the learning curve and optimizing application performance. 

Fraction 

Collection 

GPC Clean-up Systems 

Multiple 

Fractions 

Solid Phase 

Extraction 

Control 

Page 

Number 

Liquid Disposable Solid Phase Solvent Tweaks Auxiliary 

Handling Tips Extraction System 

Product #s: 210630R20 (Trilution® LH Media) 

21063020 (Trilution® LH Lifetime License) 

21063021 (Trilution® LH 1 Year License) 

Note: Requires Oracle® Database 10g Express Edition 

Automated 

GX-271 GPC 

Clean-up 

System 

0.25 – 450 mL 

(optional) 

Trilution® LC Software 30 

GX-271 

GPC/SPE 

Clean-up 

System 

0.25 – 450 mL 

(optional) 

Trilution® Software 31 

Main Menu 

Manual GPC 

Clean-up 

System 

0.25 – < 1000 mL 

(optional) 

307 Pump 

keypad 

32 

Method in Method Builder Screen 

Over 70 Gilson-supplied tasks 

enable quick and efficient 

method programming. 

Quick and effortless 

instrument setup, bed layout 

and method creation. 

Sample list with spreadsheet 

functionality and graphical 

run simulation. 

SS Dispense Task Property Page 

Run methods in either 

batch or sequential mode to 

optimize performance. 

Manual control for performing 

routine tasks such as priming 

instruments and troubleshooting. 

Graphical custom task creation and 

cartridge monitoring. 

Simulation Mode in Application Run Screen 

Computer Recommendation 

Processor 

Intel® Pentium® 4 

(2.8 GHz) 

RAM 

1 GB 

Fixed Drive 

80 GB, available 

space: 4 GB 

(6 GB at install) 

Additional Drive 

DVD/CD-RW 

Video Card 

1280 x 1024 with 

24 bit True Color 

Serial Ports 

2 

Operating System 

Microsoft® Windows® XP Professional SP2 or later 

Microsoft® Windows® 2000 SP4 

Gilson’s gel permeation chromatography (GPC) 

clean-up systems are an ideal solution for postextraction 

clean-up of interfering substances from 

environmental samples, food products and animal 

tissue, prior to analysis by GC, GC/MS, HPLC or LC/MS. 

Failure to remove these interfering compounds can 

cause poor analytical results, shorten column life and 

increase analytical downtime. 

Gilson GPC Clean-up Systems meet clean-up guidelines set forth by the USEPA, USFDA, USDA, USGS, ISO, 

CFIA, AOAC International and European Union (EN 1528, EN 12393, S19) requirements. 

Visit www.gilson.com or call 608-836-1551 for more details on Gilson GPC Clean-up Systems. 

29


The Automated GX-271 GPC Clean-up System from Gilson is capable of up to 80 sample 

extractions with large fraction collection capacity into an assortment of vessels. 

Gilson’s newly released Automated GX-271 GPC/SPE Clean-up System provides SPE capabilities in 

addition to GPC for sample extract purification; a multi-purpose sample preparation system. 

Automated GPC Systems Bed Capacity 

Sample 

Quantity Description GPC 

Collection Rack 

Product # 

36 38 x 200 mm (170 mL) collection tubes Code 320 Rack 260440050 

85 25 x 200 mm (70 mL) collection tubes Code 321 Rack 260440051 

13 61 x 137 mm (250 mL) Boston Round bottles Code 322 Rack 260440052 

8 Labconco RapidVap (450 mL) flat-bottom evaporation flasks Code 323 Rack 260440053 

20 Caliper TurboVap II (200 mL) evaporation tubes Code 324 Rack 260440054 

8 Labconco RapidVap (450 mL) evaporation flasks, with stem Code 325 Rack 260440055 

50 Caliper TurboVap II (50 mL) evaporation tubes Code 326 Rack 260440056 

32 Labconco RapidVap (100 mL) flat-bottom evaporation flasks Code 327 Rack 260440057 

23 49 x 114 mm (125 mL) Boston Round bottles Code 328 Rack 260440058 

12 Pyrex 4100 (125 mL) flat-bottom flasks for rotary evaporators Code 329 Rack 260440059 

8 Pyrex (250 mL) flat-bottom flasks for rotary evaporators Code 331 Rack 260440060 

Sample 

Quantity Description GPC 

Sample Rack 


80 13 x 100 mm (8 mL) tubes Code 343 Rack 260440025 

44 16 x 100 mm (12 mL) tubes Code 344 Rack 260440049 

Compatible with various GPC 

columns from low pressure glass 

to high pressure stainless steel. 


Automated GPC Systems 

Flow Rate 

0.01 – 10 mL/min 

Injection Volume 

20 µL – 5 mL 

Wavelength 

254 or 280 nm 

Maximum Pressure 

4,000 psi (276 bar) 

Mobile Rack technology enables 

programmed switching from drain 

and collect positions to completely 

automate the entire SPE process from 

condition to load, wash and elute. 

The GX Direct Injection Module reduces sample waste and 

carryover, which prevents the need to overfill the sample loop. 

Sample Capacity 

SPE: 60 (3 mL), 45 (6 mL) 

GPC: 80 

Positive Pressure 

elution increases 

reproducibility 

and precision. 

Product #s: 21110000 (Automated GX-271 GPC Clean-up System, with 112 UV Detector) 

21110001 (Automated GX-271 GPC Clean-up System) 

Product #: 21110004 (Automated GX-271 GPC/SPE Clean-up System) 

Visit www.gilson.com or call 608-836-1551 for more details on Gilson GPC Clean-up Systems. 

31


Gilson’s Manual GPC Clean-up System is a simple and economical 

solution within a small footprint requiring minimal bench space. 


Solid Phase Extraction Systems 

Flow Rate 

0.01 – 10 mL/min 

Maximum Fractions 

10 separate 


20 µL – 5 mL 

Wavelength 

254 or 280 nm 

Maximum Pressure 

4,000 psi (276 bar) 

Liquid 

Handler 

Multiple 

Collection 

Positive 

Pressure 

Elution 

Cartridge Sizes 

1 mL 3 mL 6 mL Microplate 

Page 

Number 

GX-271 

ASPEC 

34 

GX-274 

ASPEC 

35 

Quad-Z 215 

Liquid 

Handler 

for SPE 

36 

Manual injection 

valve coordinates 

injection time. 

Collection of multiple fractions from the 

same sample extract is done with the 

VALVEMATE® II Valve Actuator. 

SPE 215 

System 

37 

Integrate a 112 UV Detector for 

EPA method compatibility. 

Code 300 

Series 

Racks 

37 

Keypad provides 

simple setup 

and control of 

the system for 

time-based 


Product #s: 21110002 

(Manual GPC Clean-up System, 

with 112 UV Detector) 

21110003 (Manual GPC Clean-up System) 

Automate solid phase extraction 

(SPE) processes with Gilson’s 

complete instrument line. From 

96-well extraction plates to 

1, 3 or 6 mL cartridges, Gilson 

automated SPE solutions meet 

any application need. 

Conditioning Loading Sample Washing Eluting 

Analyte 

Interferences 

Visit www.gilson.com or call 608-836-1551 for more details on Gilson SPE Systems. 

33


The GX ASPEC Series provides rugged and reliable 

automation of SPE applications utilizing 1, 3 or 6 mL 

cartridges. 


GX-27X ASPEC Series 

The GX-274 ASPEC is an ideal 

solution for mid to high-throughput 

sample clean-up and extraction. 

The GX-271 ASPEC is truly the solid solution for low 

to mid-throughput sample clean-up and extraction. 

A utomated 

S olid 

P hase 

E xtraction 

C artridges 

Utilizes Mobile Rack technology to 

completely automate the 

SPE process by increasing capacity 

and flexibility while performing 

both SPE and liquid handling. 

Cartridge Capacity 

108 (1 mL), 60 (3 mL), 45 (6 mL) 

Accuracy 

98% (100 μL – 25 mL) 

Optional safety 

shield available. 

Positive Pressure Modes 

syringe or gas cylinder/source 


59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in) 

Sealing Cap technology 

enables Positive 

Pressure SPE, ensuring 

better reproducibility 

and higher precision in 

comparison to vacuum 

based instruments. 

Process four SPE cartridges 

simultaneously for 

increased throughput. 

Mobile Rack technology enables the entire SPE process to be 

automated from the initial condition to the final elution and 

transfer while allowing access to cartridges and compounds 

of interest. The linear rail system permits automated method 

development and method optimization in which all or selected 

steps are collected and evaluated. 

On-bed transfer ports allow access to two additional large 

volume solvent reservoirs; incorporating a VALVEMATE® II 

provides access to ten off-bed solvents. 

On-bed access 

to four 500 mL or 

700 mL reservoirs. 

Built-in pressure sensing on each fluid line 

monitors cartridge pressure and responds to 

blockage by activating an error handling method. 

Positive Pressure elution increases reproducibility 

and precision due to process consistency. 

Choose between the 406 Single or, for more flexibility, the 406 Dual Syringe Pump for both 

large and small volume capabilities. Both pumps have built-in pressure sensing capabilities 

for detecting clogged fluid lines and monitoring cartridge pressure on each fluid line. 

Integrate a GX Direct Injection Module 

for on-line injection onto HPLC or 

LC/MS following SPE clean-up. 

Product #s: 2614007 (right) (GX-271 ASPEC, with 406 Single Syringe Pump) 

2614008 (left) (GX-271 ASPEC, with 406 Dual Syringe Pump) 



261356 (GX Direct Injection Module,1/16”, analytical SS) 

261357 (GX Direct Injection Module, 1/16”, analytical PEEK) 

Product #: 2614010 

(GX-274 ASPEC, with two 406 Dual Syringe Pumps) 


35


Gilson’s Quad-Z 215 Liquid Handler is a flexible and robust system 

that performs liquid handling tasks, on-bed filtration and Positive 

Pressure SPE. 

This liquid handler accommodates various racks maximizing 

flexibility for high-throughput applications. 

Cartridge Capacity 

288 (1 mL), 144 (3 mL), 60 (6 mL) 



91 x 61 x 61 cm (36 x 24 x 24 in) 

Accuracy 

98% (100 μL – 25 mL) 

The SPE 215 System provides an automated solution for 

sample preparation, sample reconstitution and parallel 

injection onto HPLC or LC/MS systems. Additionally, the 

SPE 215 enables parallel clean-up, filtration and purification 

of combinatorial libraries and biological samples. 

Positive Pressure elution is 

achieved with Gilson’s integrated 

Sealing Foot design. 

Mobile Racks automate entire SPE 

and liquid handling processes. 

Four independent probes with independent 

variable spacing ranging from 9 to 18 mm 

for access to various vessel sizes. 

Using Code 300 Series Racks with Sealing Cap 

technology enables Positive Pressure SPE, ensuring 

better reproducibility and higher precision. 

(see Code 300 Series Rack Capacity chart on page 37) 

The SPE 215 

accommodates 

various racks 

offering sample 

preparation 

solutions for 

virtually any 

application. 

Up to eight samples can be processed every five 

minutes or 96 samples in an hour; a solution ideal 

for high-throughput sample clean-up applications. 


0.98% (100 μL) 


Accuracy 

97.1% (100 μL) 


97.8 x 61 x 64 cm (38.5 x 24 x 25.2 in) 

Product #: 25101321 (SPE 215 System) 

Gilson’s Code 300 Series Racks provide the ability 

to add SPE and filtration to Gilson liquid handlers 

including the 215 and GX Series instruments. 

Sample 

Quantity 

SPE 

Cartridge 

Code 300 Series Rack Capacity 

Collection Vessel Rack Product # 

96 1 mL tabless 96-well microplate, deep Code 300 Rack 2504300 

96 1 mL tabless 96-well microplate, shallow Code 301 Rack 2504301 

48 1 mL 12 x 32 mm (2 mL) vials Code 302 Rack 2504302 


48 3 mL 12 x 75 mm (5 mL) tubes Code 304 Rack 2504304 


20 6 mL 12 x 75 mm (5 mL) tubes Code 306 Rack 2504306 


Mobile Rack technology enables automated switching 

from drain and collect positions to accomplish the entire 

SPE process from condition to load, wash and elute, 

including selective evaluation of individual steps for 

method development and optimization available through 

N+1 movement into individual collection tubes. 

Sealing Cap 

technology 

enables Positive 

Pressure SPE 

ensuring better 

reproducibility 

and higher 

precision. 

Product #: 25101413 (Quad-Z 215 Liquid Handler for SPE ) 

(see page 51 for the Gilson Rack/Instrument Compatibility Chart) 


37


GX-281 

Liquid 

Handler 

GX-271 

Liquid 

Handler 

GX-274 

Liquid 

Handler 

Quad-Z 

215 Liquid 

Handler 

Quad-Z 215 Liquid 

Handler with 

Disposable 

Tips 

Multiple Probe 

215 Liquid 

Handler 

Multiple 

Probes 

Disposable 

Tips 


Accuracy 

Page 

Number 

< 1% (100 μL +) 98% (100 µL – 25 mL) 39 

< 1% (100 µL – 25 mL) 

98% (50 μL – 25 mL) 

98% (10 μL – 5 mL) 

0.8% (10 μL) 98.2% (10 μL) 42 

2.1% (1 µL of DMSO) 104% (1 µL of DMSO) 43 

4.3% (1 µL of DMSO) 113% (1 µL of DMSO) 43 

0.57% (500 µL) 99.2% (500 µL) 44 

40 

The GX-281 Liquid Handler is Gilson’s premier liquid handling 

instrument. The GX-281 offers unmatched versatility and performance 

for liquid handling operations including tube-to-plate transfers, serial 

dilutions, compound reconstitution and sample filtering through the 

use of Code 300 Series Racks and more. The efficient speed of the 

GX-281 allows applications to be completed quickly and reliably. The 

GX Solvent Systems offer a wide range of volumes and flow rates via 

patented syringeless technology. 

Access five off-bed solvent 

reservoirs for bulk reagent 

dispensing and multiple 

solvent rinsing protocols. 

Direct request aspirating 

and dispensing eliminates 

the time spent waiting for 

syringes to fill. 

Aliquot Dilution Mixing 

Optional integrated bar 

code scanner verifies rack 

types and positions. 


< 1% (100 µL +) 


87.5 x 76.2 x 96.5 cm (35 x 30 x 38 in) 


Volume Delivered 

Measured Volume 

25 

20 

15 

10 

5 

25 mL Transfers at 25 mL/min. 

10 mL Transfers at 25 mL/min. 

0 

0 2 4 6 8 10 12 

Transfers 

10 

7.5 

5 

2.5 

Accuracy 

98% (100 µL – 25 mL) 

Flow Rate 

1 – 200 mL/min 

5 µL Dispense 

0 

0 20 40 60 80 100 120 140 160 180 

Dispenses 

215 Liquid 

Handler 

223 Sample 

Changer 

1.37% (50 µL of DMSO) 99.7% (50 µL of DMSO) 44 

0.8% (10 µL) 98.2% (10 µL) 45 

Gilson liquid handlers are industry renowned for 

rugged, reliable performance at an economical price. 

Whether the application requires a single probe, 

multiple probes, variable spacing or disposable tips, 

Gilson’s liquid handling solutions meet all needs. 


261031 (GX-281 Liquid Handler, with bar code scanner) 

GX-281 Liquid Handler 

Bed Capacity 

Tube 

Diameter 

Sample 

Quantity 

13 mm 576 

15 mm 576 

16 mm 450 

18 mm 420 

25 mm 180 

28 mm 162 


can be accommodated 



Visit www.gilson.com or call 608-836-1551 for more details on Gilson Liquid Handlers. 

39




GX-271 Preparative Liquid Handler 

GX-271 Analytical Liquid Handler 

% CV 

1.4 

1.2 

1 

0.8 

0.6 

0.4 

0.2 

0 

Accuracy 

98% (50 µL – 25 mL) 

Flow Rate 

1 µL – 50 mL/min 


59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in) 

GX Prep Solvent System Transfers 

50 µL 

100 µL 

500 µL 

1 mL 

5 mL 

10 mL 

25 mL 

The GX-271 Liquid Handler, 

Gilson’s newest instrument, 

offers a medium capacity with 

a small footprint. The GX-271 

models are economical and robust 

liquid handlers for reformatting, 

reagent dispensing and 

performing serial dilutions. Their 

modular design allows for future 

expansion by adding injection and 

fraction collection capabilities. 

Consumes only 23" of linear 

bench space. 

Flexible bed accommodates 

five racks and virtually any 

tube, vial or microplate. 

Ethernet based 

communication provides 

better connectivity. 

14 

12 

10 

8 

% CV 

6 

4 

2 

0 

Accuracy 

98% (10 µL – 5 mL) 

Flow Rate 

1 µL – 5 mL/min 


59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in) 

GX Analytical Solvent System Transfers 

1 µL 

10 µL 

1 mL 

5 mL 

Volume 

(see GX-271 Liquid Handler/Injector page 12) 

Volume 

GX Prep Solvent System Transfers 

Volume CV Accuracy 

50 µL 1.25% ± 2 µL 

100 µL 1.18% ± 4 µL 

500 µL 0.48% ± 7 µL 

1 mL 0.14% ± 5 µL 

5 mL 0.09% ± 16 µL 

10 mL 0.07% ± 105 µL 

25 mL 0.02% ± 46 µL 

Jet Wash Rinse technology provides 

flowing rinse for fast and efficient 

rinsing protocols utilizing up to two 

separate flowing rinse solvents. 

Gilson’s GX 

Solvent System, 

available in an 

analytical or prep 

model, features 

a dynamic range 

for volumes and 

flow rates and 

can access five 


Liquid Level Detection 

allows for detecting a 

variety of solvents as 

low as 20 µL in a 

384-well microplate. 

GX Analytical Solvent System Transfers 

Volume CV Accuracy 

1 µL 11.98% ± 300 nL 

10 µL 4.5% ± 1 µL 

1 mL 0.18% ± 20 µL 

5 mL 0.2% ± 100 µL 

The GX-271 Liquid Handler is 

available without a pump or GX 

Solvent System, allowing the 

instrument to be configured 

with a 402 Syringe Pump. 

Product #s: 2614107 (GX-271 Preparative Liquid Handler) 

2614108 (GX-271 Analytical Liquid Handler) 

2614101 (GX-271 Liquid Handler without pump) 

GX-271 Preparative and 

Analytical Liquid Handler 

Bed Capacity 

Tube 

Diameter 

Sample 

Quantity 

10 mm 540 

13 mm 300 

15 mm 220 

16 mm 220 

17 mm 220 

18 mm 220 

28 mm 70 


can be accommodated 




41


The GX-274 Liquid Handler is a mid-throughput four probe 

instrument capable of processing up to four samples (at 18 

mm fixed spacing) in parallel, increasing the efficiency of liquid 

handling applications. The GX-274 Liquid Handler provides an 

economical option with a small footprint for achieving increased 

throughput in a variety of applications. 

The GX-274 is ideal for bulk reagent dispensing when used in 

conjunction with a Minipuls Evolution® or Minipuls 3 Peristaltic 

Pump or for tube-to-plate transfers using 402 Syringe Pumps. 

Access four on-bed solvent reservoirs 

without consuming a rack position. 

Accuracy 

98.2% (10 µL) 



< 0.8% (10 µL +) 


59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in) 

Flexible rack system accommodates 

tubes, vials and microplates. 

Optional flowing rinse station with 

solvent ports provides active probe 

rinsing from off-bed solvents. 

The Quad-Z 215 Liquid Handler was designed based on 

the robust Gilson 215 Liquid Handler. This instrument 

accommodates numerous racks for maximum flexibility of 

sample transfer between tubes, vials and microplates. 

Gilson’s Quad-Z 215 Liquid Handler 

can be configured with a Disposable 

Tips option, a requirement in molecular 

biology and biochemistry applications. 

Disposable tips prevent crosscontamination, 

which saves time by 

eliminating rinsing steps. This system 

includes on-bed storage of tips, as well 

as a chute for collection of used tips. 

Flexible configuration enables use of fixed probes 

and 10 μL, 200 μL and 1 mL disposable tips. 

Accuracy 

104% (1 μL of DMSO) 


< 0.05% 

Accuracy 

113% (1 μL of DMSO) 



91 x 61 x 61 cm (36 x 24 x 24 in) 


liquid handling: 2.1% (1 μL of DMSO) 

injection: < 2.0% (500 μL syringe, partial loop); 

< 5.0% (500 μL syringe, total loop) 

Disposable Tips Option 


5 μL – 5 mL 


0.8% (10 μL +); 4.3% (1 μL of DMSO) 

A VALVEMATE® II can be 

incorporated enabling access to 

ten additional off-bed solvents. 

Integrating two 402 Dual Syringe Pumps 

provides four independently controlled 

syringes from 100 μL to 25 mL. 

For access to the various vessel sizes, 

the four independent probe variable 

spacing ranges from 9 to 18 mm. 



Product #s: 25101413 (Quad-Z Liquid Handler) 

2506163 (Quad-Z 215 Adapter Kit for Disposable Tips) 

2515154 (849 Multiple Injection Module) 



43


Select either a four or eight probe 

configuration depending on application 

and throughput requirements. 

Ideal solution for 

plate-to-plate transfers. 

The Multiple Probe 215 is a high-throughput automated 

liquid handler capable of tasks such as sample transfers, 

dilutions, mixing, reagent addition and microplate 

reformatting. The Multiple Probe 215 features patented 

Dual Rail Syringe Drive technology, accurate use of four 

or eight syringes and accommodates probes that fit all 

application requirements. 

The 223 Sample Changer is a programmable sampler for automated 

sample preparation and transfer. It provides a cost-effective solution 

for protocols such as serial dilutions, additions, sampling into vials, 

timed reactions and tube-to-tube transfers. 

The 223 Sample Changer is ideal for automated transfer of samples in 

analytical techniques like FIA and spectroscopy (UV/VIS, AA and ICP). 


0.8% (10 μL) 


Accuracy 

98.2% (10 μL) 


53.5 x 43.7 x 60 cm (21.1 x 17.2 x 24 in) 

Cross-contamination is minimized by adjusting rinse volumes and 

using liquid level sensing. 


Carryover 

< 1 ppb 


0.57% (500 µL) 

Accuracy 

99.2% (500 µL) 

Optional flow-through rinse station, available 

on the left and/or right of the support bar, 

reduces cross-contamination. 

Choose between an optional 402 Syringe Pump, 

Minipuls Evolution® or Minipuls 3 Peristaltic 

Pump to add aspirating and dispensing flexibility. 


97.8 x 61 x 56 cm (36 x 24 x 22 in) 

Product #: 25101311 (Multiple Probe 215 Liquid Handler) 

The 215 Liquid Handler is a laboratory tested workhorse 

for performing dilutions, aliquoting solutions, mixing, 

plate reformatting, reconstituting of samples and more. 

The 215 Liquid Handler has a punch strength for piercing 

thick or thin film septa and microplate mats that is 

distinguished throughout the liquid handling industry. 

Accommodates a wide variety 

of probes as well as standard 

and custom racks. 

(see page 51 for the Gilson Rack/ 

Instrument Compatibility Chart) 

Flexible open bed design allows for a variety of 

rack options including tubes, vials, microplates 

and Peltier control. 



1.37% (50 µL) 

Carryover 

< 1 ppb 

Accuracy 

99.7% (50 µL) 

Product #s: 2510121 (215 Liquid Handler) 

2510191 (215 Liquid Handler without pump) 


91 x 61 x 56 cm (36 x 24 x 22 in) 

Product #: 191015 (223 Sample Changer) 


45

Accessories 

Switching Valve Mixing Racks Heating/Cooling 

Page 

Number 

The VALVEMATE® II Valve Actuator automates HPLC and LC 

techniques by increasing the system flexibility to save time, 

increase throughput and extend column life. It is designed to 

facilitate both solvent selection and column switching. The 

VALVEMATE II increases flexibility of HPLC systems allowing 

for multiple valve and multiple column configurations. 



8.3 x 15.6 x 9.8 cm (3.3 x 6.2 x 3.9 in) 


< 180 ms port-to-port 

(depending on valve) 

VALVEMATE® II 

Valve Actuator 

Orbital Shaker 48 

RackCount 

System 

818 AutoMix 49 

47 

48 

High Pressure Valves: 

• Analytical 2-position, 6-port, stainless steel (0.016” ID ports) 

• Analytical 2-position, 10-port, stainless steel (0.016” ID ports) 

• Preparative 2-position, 6-port, stainless steel (0.030” ID ports) 

• Preparative 2-position, 10-port, stainless steel (0.030” ID ports) 

• Preparative 2-position, 6-port, PPS (0.030” ID ports) 

• Preparative 2-position, 10-port, PPS (0.030” ID ports) 

• Multi-position, 6-port, stainless steel (0.016” ID ports) 


• Multi-position, 6-port, PEEK (0.016” ID ports) 


• Multi-position, 10-port, PEEK (0.016” ID ports) 

Low Pressure Valves: 

• Multi-position, 6-port, PPS (0.030” ID ports) 



6 Column Plumbing Schematic 

852/853/854 

Peltier 

Racks 

40ºC 

4ºC 

49 

242/542 

Peltier 

Racks 

Complementing robust and flexible instruments, 

Gilson’s accessories optimize instrumentation to fit 

application requirements for the ultimate solution. 

40ºC 

4ºC 

50 

Multi-directional 

keypad control 

with valve position 

LED display. 

Contact closure control for 

home and switch valve position. 

Fast valve switching times 

minimize pressure spikes 

and carryover. 

Product #: 331052-AB (VALVEMATE® II Valve Actuator) 

Visit www.gilson.com or call 608-836-1551 for more details on Gilson Accessories. 

47

Accessories 

The Gilson Orbital Shaker is specifically 

designed to satisfy liquid handling 

needs for on-line or off-line vortexing 

and mixing. It is a fully functional, 

stand-alone instrument equipped with 

a single-stepper motor. 

Designed for Gilson’s 215 Series instruments, the 818 AutoMix provides fully 

automated, software-driven mixing and sampling. Typical applications that use 

the AutoMix include mixing whole blood samples, immiscible liquids and more. 

Mix an entire rack of samples 

with oscillating mixing. 


Mixing Speed/Accuracy 

10 rpm: ± 0.5 rpm 

20 rpm: ± 1 rpm 

Rack Capacity 

(1) Code 200 Series Rack 


22.9 x 50.8 x 16.5 cm (9 x 20 x 6.5 in) 


Circular Rotational Speed 

20 – 720 rpm 

Rack Capacity 

(1) Code 200 Series Rack 


11.8 x 31.6 x 4.7 cm (4.65 x 12.44 x 1.85 in) 

The Orbital Shaker can be placed 

on the bed of the GX-281 or 215 

Series instruments using the 

optional mounting plate kits. 

Product #: 251520 (818 AutoMix) 

Product #: 251711 (Orbital Shaker) 

Gilson’s RackCount System, numbered overlays that fit securely on the 

top of racks, provides a quick and effortless bed location reference for 

specific samples or collected fractions on a Gilson liquid handler. 

RackCount overlays can be used with new rack purchases 

or added to existing Gilson Code 200 Series Racks. 

They have sequentially numbered fraction collection 

positions for five complete rack positions and one position 

for sample locations. 

The numbering patterns are solvent resistant and compatible 

with most normal and reversed-phase solvents. 

Gilson offers flexible, accurate and programmable racks for heating and 

cooling valuable samples; cooling to reduce evaporation and maintain 

sample integrity, heating to facilitate reactions and improve kinetics. 

Gilson offers a variety of standard 

and custom rack configurations. 

Product #s: 2514852 (far left) (852 Peltier Rack) 

2514853 (center left) (853 Peltier Rack) 

2514854 (center right) (854 Peltier Rack) 

2515850 (far right) (Peltier Controller) 

Up to five racks can be controlled from a single programmable 

Peltier controller integrated with a system or as a stand-alone unit. 

Precise temperature control from 4 to 40°C. 


Rack Capacity 

852: (2) 96-well microplates, shallow 

853: (96) 12 x 32 mm (2 mL) flat-bottom vials 

854: (2) 96-well microplates, deep 


11.9 x 33.8 x 16.8 cm (4.7 x 13.3 x 6.6 in) 

Visit www.gilson.com or call 608-836-1551 for more details on Gilson Accessories. 

49

Accessories 

Reference 

Gilson Peltier Racks are individually 

controlled to cool and heat microplates 

on the bed of a Gilson liquid handler. 

The racks provide accurate temperature 

control from 4 to 40˚C. 

Product #s: 

25146331 (left) (242 Peltier Rack) 

2514542 (center) (542 Peltier Rack) 

2515331 (right) (215 Peltier Controller) 

Gilson offers thousands of standard and custom racks to meet the needs 

of any and all applications. A variety of vessels can be accommodated 

including tubes, vials, microplates and microchips. 

The combination of a 542 and a 242 

Peltier Rack can accommodate over 

1,000 samples on a 215 Liquid Handler. 

Rack Capacity 



A single Peltier controller is able to 

power a 542 and a 242 Peltier Rack 

or up to five 242 Peltier Racks. 



242: 11.4 x 30.5 x 12.4 cm (4.5 x 12 x 4.9 in) 

542: 50.1 x 33.6 x 11.4 cm (20.1 x 13.3 x 4.5 in) 

Rack Code 

Gilson Rack/Instrument Compatibility Chart 

Rack 


Capacity Description 

Microplate 

Vessel Capacity (mL) 

0 270430 80 - 12 x 32 mm (2 mL) vials 2 80 12 32 X X X 

1 12040101 80 - 13 x 65 mm (6 mL) or 13 x 100 mm (9 mL) tubes 6 or 9 80 13 65 or 100 X 

2 130402 119 - 6 x 50 mm (1 mL) tubes 1 119 6 50 X 


7 2707401 1 - 96-well microplate, shallow flat-bottom X 1 X 

8 270438 120 - 6 x 32 mm (0.3 mL) vials 0.3 120 6 32 X X 

9 270439 120 - 7 x 40 mm (0.7 mL) vials 0.7 120 7 40 X X 

11 170413 105 - 13 x 100 mm (9 mL) tubes 9 105 13 100 X 


15 170415 1 - 96-well microplate, shallow X 1 X 

16 170416 4 - 96-well microplates, shallow X 4 X 

16D 170416D 4 - 96-well microplates, deep X 4 X 

17 170418 

8 - 24, 48 or 96-well microplates, 

Whatman Deep Polyfiltronics Plates 

Vessel Quantity 

Vessel Diameter 

Vessel Height 

GX-281 LH/Inj 

X 8 X 

20 150425 108 - 10 x 100 mm (4.5 mL) tubes 4.5 108 10 100 X X X X X X X X X X 

21 150422 60 - 13 x 100 mm (9 mL) tubes 9 60 13 100 X X X X X X X X X X 

22 150424 44 - 18 x 150 mm (25 mL) tubes 25 44 18 150 X X X X X X X X 

22U 150498 44 - 10 x 100 mm (4.5 mL) to 18 x 180 mm (32 mL) tubes 4.5 to 32 44 10 to 18 100 to 180 X X X X X X X X 

23 150426 44 - 17 x 55 mm (6.8 mL) scintillation vials 6.8 44 17 55 X X X X X X X X X X 

23W 270433 44 - 15 x 45 mm (4 mL) WISP vials 4 44 15 45 X X X X X X X X 

24 150427 14 - 28 x 57 mm (20 mL) scintillation vials 20 14 28 57 X X X X X X X X X X 

28 150420 108 - 10 x 75 mm (3.5 mL) tubes 3.5 108 10 75 X X X X X X X X X X 

29 150429 60 - 12 x 75 mm (5 mL) or 13 x 75 mm (7 mL) tubes 5 or 7 60 12 or 13 75 X X X X X X X X X X 

29LE 2704342 60 - (1.5 mL) Eppendorf vials 1.5 60 X X X X X X 

29SE 2704341 60 - (0.5 mL) Eppendorf vials 0.5 60 X X X X X X 

30 2704430 60 - 12 x 32 mm (2 mL) vials 2 60 12 32 X X X X 

30P 2704530P 60 - 12 x 32 mm (2 mL ) vials 2 60 12 32 X X 

31 2704431 108 - 7 x 40 mm (0.7 mL) vials 0.7 108 7 40 X X X 

31P 2704531P 108 - 7 x 40 mm (0.7 mL) vials 0.7 108 7 40 X 

32 2704432 60 - 13 x 65 mm (6 mL) or 13 x 100 mm (9 mL) tubes 6 or 9 60 13 65 or 100 X X X X 

33 2704433 14 - 28 x 57 mm (20 mL) scintillation vials 20 14 28 57 X X X X 

33P 2704533P 14 - 28 x 57 mm (20 mL) scintillation vials 20 14 28 57 X X 

GX-271 LH/Inj 

215 LH/Inj 

Multiple Probe 215 LH/Inj 

GX-274 LH 

GX-271 LH 

223 Sample Changer 

Quad-Z 215 LH 

Quad-Z 215 (Disposable Tips) 

GX-274 ASPEC 

GX-271 ASPEC 

SPE 215 

prepFC 

FC 204 

FC 203B 

Orbital Shaker 

Visit www.gilson.com or call 608-836-1551 for more details on Gilson Instruments and Software. 

51

Reference 

Gilson Rack/Instrument Compatibility Chart continued 

Rack Code 

Rack 



Microplate 

34 2704434 36 - 15 x 45 mm (4 mL) WISP vials 4 36 15 45 X X 

34P 2704534P 36 - 15 x 45 mm (4 mL) WISP vials 4 36 15 45 X 

35P 2704535P 

71 - 12 x 32 mm (2 mL) vials + 

1 - 28 x 60 mm (20 mL) vial 

36P 2704536P 

120 - 7 x 41 mm (0.7 mL) vials + 

1 - 28 x 60 mm (20 mL) vial 




Vessel Height 

GX-281 LH/Inj 

GX-271 LH/Inj 

215 LH/Inj 


GX-274 LH 

GX-271 LH 

2 + 20 71 + 1 12 + 28 32 + 60 X 

0.7 + 20 120 + 1 7 + 28 41 + 60 X 

37 2704437 56 - 12 x 32 mm (2 mL) vials + 1 - 28 x 60 mm (20 mL) vial 2 + 20 56 + 1 12 + 28 32 + 60 X 

81 1904301 2 - 96-well microplates, shallow X 2 X 

91 17041950 11 - Kimax or Pyrex (500 mL) bottles 500 11 X 

92 17041951 8 - Kimax or Pyrex (1000 mL) bottles 1000 8 X 

121 260440086 60 - 13 x 100 mm (9 mL) tubes 9 60 13 100 X X X X 

122 260440087 39 - 16 x 100 mm (12 mL) tubes 12 39 16 100 X X X X 

143 2954843 20 - (3 mL) DECs 3 20 X X X X X 

146 2704146 8 - (6 mL) DECs 6 8 X X X X X 

200 2504600 96 - 13 x 100 mm (9 mL) Vacutainer tubes 9 96 13 100 X X X X X X X X X 

201 2504601 

2 - 96-well microplates + 2 - (1.5 mL) microcentrifuge 

tubes + 2 - 13 x 100 mm (9 mL) tubes 

201H 2504601H 

2 - 96-well microplates, w/covers + 2 - (1.5 mL) 

microcentrifuge tubes + 2 - 13 x 100 mm (9 mL) tubes 

202 2504602 

96 - 10.25 x 47 mm (1.5 mL) microcentrifuge tubes or 

(2.5 mL) Vacutainer tubes 

X 1.5 + 9 2 + 2 +2 13 100 X X X X X X X X X X X X 

X 1.5 + 9 2 + 2 + 2 13 100 X X X X X X X X X X X X 

1.5 or 2.5 96 10.25 47 X X X X X X X 

203 2504603 96 - 10.25 x 64 mm (3 mL) tubes 3 96 10.25 64 X X X X X X X X X 

204 2504604 27 - 28 x 57 mm (20 mL) scintillation vials 20 27 28 57 X X X X X X X X X 

204F 2504604F 24 - 28 x 57 mm (20 mL) scintillation vials 20 24 28 57 X X 

205 2504605 

2 - 96-well microplates, deep + 2 - (1.5 mL) Eppendorf 

vials + 2 - 13 x 100 mm (9 mL) tubes 

205H 2504605H 

2 - 96-well microplates, deep w/covers + 2 - (1.5 mL) 

Eppendorf vials + 2 - 13 x 100 mm (9 mL) tubes 





208 2504608 70 - 18 x 150 mm (25 mL) tubes 25 70 18 150 X X X X X X X X 

209 2504609 96 - 12 x 32 mm (2 mL) vials 2 96 12 32 X X X X X X X X X 


211 2504611 9 - 48 x 113 mm (125 mL) bottles 125 9 48 113 X X X X X X X X X 

211F 2504611F 8 - 48 x 113 mm (125 mL) bottles, w/funnel drain 125 8 48 113 X X 

211H 2504611H 9 - 48 x 113 mm (125 mL) bottles, w/cover 125 9 48 113 X X X X X X X X X 

212 2504612 

48 - 13 x 100 mm (9 mL) tubes + 

48 - 13 x 75 mm (7 mL) Vacutainer tubes 

9 + 7 48 + 48 13 + 13 100 + 75 X X X X X X X X X 


Quad-Z 215 LH 


GX-274 ASPEC 

GX-271 ASPEC 

SPE 215 

prepFC 

FC 204 

FC 203B 


Rack Code 

Rack 



213 2504613 

37 - 16 x100 mm (12 mL) tubes + 

37 - 16 x 75 mm (10 mL) Vacutainer tubes 

214 2504614 

48 - 10.25 x 47 mm (2.5 mL) tubes + 

48 - 10.25 x 64 mm (3 mL) Vacutainer tubes 

Microplate 




Vessel Height 

GX-281 LH/Inj 

GX-271 LH/Inj 

215 LH/Inj 

12 + 10 37 + 37 16 + 16 100 + 75 X X X X X X X X 

2.5 + 3 48 + 48 

10.25 + 

10.25 


GX-274 LH 

GX-271 LH 


Quad-Z 215 LH 


GX-274 ASPEC 

GX-271 ASPEC 

SPE 215 

prepFC 

FC 204 

FC 203B 

47 + 64 X X X X X X X X 

216 2504616 60 - 15 x 45 mm (4 mL) WISP vials 4 60 15 45 X X X X X X X X 

217 2504617 96 - 10 x 75 mm (4 mL) tubes 4 96 10 75 X X X X X X X X X X X X 

218 2504618 

2 - 96-well microplates, shallow or deep + 8 - 10 x 75 mm 

(4 mL) tubes; also holds Beckman modular reservoirs 

X 4 2 + 8 10 75 X X X X X X X X X X X 

219 2504619 27 - 30 x 95 mm (40 mL) vials 40 27 30 95 X X X X X X X X X 

220 2504620 70 - 17 x 62 mm (7 mL) vials 7 70 17 62 X X X X X X X 

222 2504622 27 - 29 x 115 mm (50 mL) conical bottom tubes 50 27 29 115 X X X X X X X X X 

222F 2504622F 

24 - 29 x 115 mm (50 mL) conical bottom tubes, 

w/ funnel drain 

50 24 29 115 X X 

223 2504623 96 - 16 x 100 mm (12 mL) tubes, w/screw caps 12 96 16 100 X X X X X X X 

224 2504624 192 - 8.5 x 41 mm (27 mL) vials 27 192 8.5 41 X X X X X X X 

225 2504625 30 - 25 x 150 mm (45 mL) vials 45 30 25 150 X X X X X X X 

226 2504626 96 - 15 x 45 mm (4 mL) WISP vials 4 96 15 45 X X X X X X X 

228 2504628 4 - (500 mL or 700 mL) bottles 500 or 700 4 X X X X X X X X X X X 

234 2504634 2 - Tipack boxes (10 µL or 200 µL tips) 2 X 

235 2504635 2 - Tower Pack boxes (10 µL or 200 µL tips) 2 X 

237 2504637 2 - 384-well microplates X 2 X X X X X X X X X X X X 

239 2504639 40 - 25 x 200 mm (70 mL) tubes 70 40 25 200 X X X X X X 

242 25146331 2 - 96-well microplates, shallow X 2 X X X X X X X X X X 

300 2504300 

96 - (1 mL) cartridges + 

1 - 96-well microplate, deep 

301 2504301 


1 - 96-well microplate, shallow 

302 2504302 


48 - 12 x 32 mm (2 mL) vials 

303 2504303 


48 - 12 x 32 mm (2 mL) vials 

X 1 96 + 1 X X X X X X X X X 

X 1 96 + 1 X X X X X X X X X 

1 + 2 48 + 48 12 32 X X X X X X X X X 

3 + 2 48 + 48 12 32 X X X X X X X X X 

304 2504304 48 - (3 mL) cartridges + 48 - 12 x 75 mm (5 mL) tubes 3 + 5 48 + 48 12 75 X X X X X X X X X 

305 2504305 


32 - 15 x 45 mm (4 mL) vials 

3 + 4 32 + 32 15 45 X X X X X X X X X 

306 2504306 20 - (6 mL) cartridges + 20 - 12 x 75 mm (5 mL) tubes 6 + 5 20 + 20 12 75 X X X X X X 

307 2504307 


20 - 15 x 45 mm (4 mL) vials 

6 + 4 20 + 20 15 45 X X X X X X 

330 260440031 60 - 12 x 32 mm (2 mL) vials 2 60 12 32 X X X 

332 260440079 44 - 18 x 150 mm (25 mL) tubes 25 44 18 150 X X X X X 

334 260440081 14 - (40 mL) scintillation vials 40 14 X X X X X 



53

Reference 

Rack Code 

Gilson Rack/Instrument Compatibility Chart continued 

Rack 



Microplate 


335 260440083 48 - 15 x 45 mm (4 mL) WISP vials 4 48 15 45 X X X X X X 

336 260440094 2 - 96-well microplates, shallow or deep X 2 X X X X X 


338 260440106 64 - 12 x 32 mm (2 mL) vials 2 64 12 32 X X X X X 

341 260440039 108 - 10 x 75 mm (3.5 mL) tubes 3.5 108 10 75 X X X X X 



371 2604701 36 - (1 mL) DECs 1 36 X X X X 

373 2604702 20 - (3 mL) DECs 3 20 X X X X 

376 2604703 15 - (6 mL) DECs 6 15 X X 

502 2504652 100 - 12 x 32 mm (2 mL) vials (1 - HP 1100LC tray) 2 100 12 32 X X 


505H 2504651H 10 - 96-well microplates, shallow or deep w/covers X 10 X X X X X 

517 2504653 17 - 96-well microplates, shallow or deep X 17 X X 

542 2514542 10 - 96-well microplates, shallow X 10 X X X X 

821 2504821 48 - (1 mL) tabbed SPE cartridges 1 48 X 

822 2504822 96 - (1 mL) tabless SPE cartridges 1 96 X 

823 2504823 48 - (3 mL) tabless SPE cartridges 3 48 X 

824 2504824 

825 2504825 

852 2514852 

1 - 96-well wide SPE plate, compatible with 

porvair-style plates such as Varian & Waters 

1 - 96-well narrow SPE plate, compatible with 

plates such as 3M & Ansys Spec. 

2 - 96-well Becton Dickinson Falcon 

"U-bottom" microplates, shallow 



Vessel Height 

GX-281 LH/Inj 

GX-271 LH/Inj 

215 LH/Inj 

1 X 

1 X 

X 2 X X X X X X X X 

853 2514853 96 - 12 x 32 mm (2 mL) vials, flat-bottom 2 96 12 32 X X X X X X 

854 2514854 2 - 96-well Beckman or Ritter microplates, deep X 2 X X X X X X X X 

VR 2514701 

SPE extraction plates: Waters OASIS®, 3M Empore and 

Varian VersaPlate 

M 1907152 223 Maldi Rack, 1 - Bruker MALDI Plate X 

Gilson Probe Product Number Identification 

Gilson probes are laser engraved with their product number on the collar of each probe 

providing a quick and effortless identification reference for re-ordering and support. 


GX-274 LH 

GX-271 LH 


Quad-Z 215 LH 

X 


GX-274 ASPEC 

GX-271 ASPEC 

SPE 215 

prepFC 

FC 204 

FC 203B 


Probes 

Gilson offers many different types of probes, probe tips and coatings to meet any and all application needs providing 

solutions for all liquid handling requirements. 

Tip Design Description Features 

Flat The tip is cut perpendicular to the length of the probe. · Aspirates the last drop of liquid 

· Does not pierce septum 

· No injection 

Constricted 

The tip of the probe has a smaller outer diameter than the 

rest of the shaft. Constricted-tip probes accommodate 

unique tip designs optimizing every application. 

· Compatible with injection ports 

Beveled The tip is cut at an angle, leaving a bevel. · Sharp tip enables septum-piercing 


Spring Loaded Low volume, touch-off dispensing. · Ideal for MALDI spotting applications 

· Spring loaded probe removes any potential of 

damaging spotting surface 

Vented 

Side-entry 

Deflected 

Two styles: 

1. A shallow groove cut in the probe shaft, above the tip 

(shown). 

2. Two probes are assembled concentrically, creating two 

separate channels, the inner channel for liquid and the 

outer channel for gas. 

Vented probes may have different tip designs. 

The liquid flow path opens on the side of the probe, 

not at the tip. 

Based on the beveled tip design, the probe tip is curved 

and very sharp. 

· Sharp-tip enables septum-piercing 

· Vent allows pressures inside and outside a sealed vessel to 

equilibrate as liquid is dispensed or aspirated 

· Recommended for thick septa 

· Prevents coring of septa 


· Not recommended with limited volume 

· Sharp-tip enables septum-piercing 

· Tip pushes septa out of the way as it 

pierces to prevent coring 

Conical Designed for PEEK injection ports. · Capable of piercing thin septa 

· Aspirates the last drop of liquid 


55

Reference 

Gilson Probe Selection Guide 

Product # Septum-piercing Tip Shape Material 

175 mm Z-arm 

Dimensions 

(mm, L x OD x ID) 

Injection Compatibility 

2507214 Constricted, flat Stainless steel 269 x 1.8 x 1.4 GX Z-Injection Module 

2507215 Constricted, flat Stainless steel 269 x 1.3 x 0.8 

2507216 

Beveled, 

level-sensing 

Stainless steel 

269 x 1.5 x 1.1 

Tip: 1.6 x 1.2 x 0.8 

819 (requires Injection 

Port #25051015) 

849 (requires Injection Port 

#25051213) 

GX Direct Injection Module 

(requires Injection Port 

#26035410) 


Port #2954674) 

849 #25051215 

Seal #2954674 



#26035410) 

2507247 X Beveled Stainless steel 323 x 1.8 x 0.8 215 SPE 

2507249 

2507253 Micro septum 

Conical, 

micro-volume 

Beveled, levelsensing 

Stainless steel 269 x 2.4 x 0.4 215 


269 x 1.5 x 0.4 

Tip: Con Bev Micro 0.6 


Port #270728) 

2507254 Flat, level-sensing Stainless steel 269 x 1.8 x 1.4 GX Z-Injection Module 

2507255 


Stainless steel 269 x 1.5 x 0.4 

25073645 Beveled Stainless steel 269 x 1.3 x 0.8 

2507555 Flat 

251646 Inert Gas 

Probe 

Stainless steel, 

Teflon® coated 

(plastic tip) 

272.5 x 2.7 x 0.8 

Tip: 10 


Port #295640) 

849 #25051215 



#26035411) 


Port #250510153) 


Port Bar #250512862) 

GX Z-Injection Module 

X Beveled Stainless steel 317 x 1.8 x 0.8 GX Z-Injection Module 

25075551 Flat Stainless steel 256.6 x 2.7 x 0.8 GX Z-Injection Module 

26017050 X Beveled Stainless steel 

125 mm Z-arm 

269 x 2 x 0.8 

Tip: 1.5 OD x 0.8 

2507234 X Side-entry Stainless steel 274 x 1.8 x 0.8 


2507236 X Side-entry Stainless steel 272.5 x 1.5 x 0.8 



Port #2954640) 



#26035411) 


Port #25051015) 



Port #25051015) 



Port #25051015) 



Port #25051015) 


Instrument Compatibility 

GX-271, GX-274 GX-281, 

215, 215 SW, 

Multiple Probe 215 

GX-271, GX-274, GX-281, 

215, 215 SW, 

Multiple Probe 215, 

Quad-Z 215 

GX-271, GX-274, GX-281, 

215, 215 SW, 


Quad-Z 215 

GX-271, GX-274, GX-281, 

Quad-Z 215 

GX-271, GX-274, GX-281, 

215, 215 SW, 

Quad-Z 215 

GX-271, GX-274, GX-281, 

GX-271 ASPEC, 

GX-274 ASPEC, 

215, 215 SW, 

Quad-Z 215 

GX-271, GX-274, 215, 

215 SW, 


Quad-Z 215 

GX-271, GX-274, GX-281, 

215 

GX-271, GX-274, GX-281, 

215 

GX-271, GX-274, GX-281, 

215 

GX-271, GX-274, GX-281, 

215, 215 SW 

GX-271, GX-274, GX-281, 

215 

GX-271, GX-274, GX-281, 

215 

GX-271, GX-274, GX-281, 

215 

GX-271, GX-274, GX-281, 

215 


125 mm Z-arm continued 

2507242 X Deflected 

2507243 X Deflected 


Teflon® interior 


Teflon® interior 

Dimensions 



274 x 1.8 x 0.8 GX Z-Injection Module 

274 x 1.8 x 0.8 GX Z-Injection Module 

2507244 X Deflected Stainless steel 274 x 1.8 x 0.8 GX Z-Injection Module 

2507245 X Deflected Stainless steel 274 x 1.8 x 0.8 GX Z-Injection Module 

2507248 

2507252 Micro septum 

Conical, microvolume 

Constricted, 

beveled 

Stainless steel 220 x 2.1 x 0.4 841 215 


220 x 1.5 x 0.4 

Tip: 10 x 0.7 x 0.4 

2507256 X Beveled Stainless steel 221 x 1.5 x 0.4 


27067361 Beveled Stainless steel 220.5 x 1.5 x 1.1 



221 x 2 x 0.8 

Tip: 2.1 x 1.5 x 0.8 


56 mm Z-arm 

496215 Flat Stainless steel 51 x 0.7 x 0.4 


Port #25051213) 

849 #250512861 


Port #2954640) 



#26035411) 


Port #25051015) 


Port Bar #250512862) 



#26035410) 


Port #2954640) 

849 #250512863 



#26035411) 


Port #2954640) 

849 #250512863 



#26035411) 


Port #2954640) 



#26035411) 


Port #2954640) 

849 #250512863 



#26035411) 

27067251 X Beveled Stainless steel 103 x 0.7 x 0.4 Requires Injection Port #2954645 231 XL 

27067113 Constricted, flat Stainless steel 93.5 x 1.5 x 1.1 


27067133 X Beveled Titanium 95 x 0.7 x 0.42 

123 mm Z-arm 

27067211 Beveled Stainless steel 160.5 x 1.5 x 1.1 

Requires Injection Port #270728 

& Probe Sleeve #27067132 


Requires Sleeve #27067132 

27067212 X Side-entry Stainless steel 160.5 x 1.5 x 1.1 Requires Injection Port #2954645 


GX-271, GX-274, GX-281, 

215 

GX-271, GX-274, GX-281, 

215 

GX-271, GX-274, GX-281, 

215 

GX-271, GX-274, GX-281, 

215 

215, Multiple Probe 215 

GX-271, GX-274, GX-281, 

GX-271 ASPEC, 

GX-274 ASPEC, 

215 

GX-271, GX-274, GX-281, 


GX-271, GX-274, GX-281, 


GX-271, GX-274, GX-281, 


232 XL/233 XL 

GX-271, GX-274, GX-281, 


(183 mm arm) 

GX-271, GX-274, GX-281, 


(183 mm arm) , GX-271 

GPC Systems 

GX-271, GX-274, 

223, 231 XL 

223, 231 XL/232 XL/ 

233 XL 

223, 231 XL/232 XL/ 

233 XL 

223, 221 XL, 

231 XL/232 XL/233 XL, 

ASTED XL 

27067213 Tapered, flat Stainless steel 160.5 x 1.5 x 1.1 223, 221 XL 


57

Reference 

Gilson Probe Selection Guide continued 


123 mm Z-arm continued 

27067217 Flat 

27067218 Flat 


(plastic tip) 


(plastic tip) 

Dimensions 


157.5 x 0.9 (18 g) 

Tip: 10 

157.5 x 0.9 (22 g) 

Tip: 10 

27067231 Tapered, beveled Stainless steel 162 x 0.7 x 0.42 


27067237 X Beveled Titanium 162 x 0.7 x 0.42 








27067238 X Beveled Stainless steel 162 x 0.7 x 0.4 Requires Injection Port #270728 

27067272 Tapered, beveled Stainless steel 160.5 x 1.5 x 1.1 Requires Injection Port #2954645 

27167215 

Double probe kit 

X Beveled Stainless steel 

27367513 Flat 

183 mm Z-arm 

27067361 



(plastic tip) 

probe 1: 181.5 x 1.5 x 1.1 

probe 2: 169.5 x 2.5 x 1.1 

157.5 x 0.9 

Tip: 10 

Stainless steel 220.5 x 1.5 x 1.1 


Port #2954640) 

232 XL/233 XL (requires 

Injection Port #2954645) 


223 

223 

223, 

231 XL/232 XL/233 XL, 

ASTED XL 

223, 

231 XL/232 XL/233 XL, 

ASTED XL 

231 XL, 

ASTED XL 

223, 

231 XL/232 XL/233 XL, 

ASTED XL 

223, 

231 XL/232 XL/233 XL, 

ASTED XL 

215, 223, 

232 XL/233 XL 

27067362 X Side-entry Stainless steel 220.5 x 1.5 x 1.1 Requires Injection Port #2954645 223, 

232 XL/233 X 

27067373 

Constricted, flat, 

level-sensing 


221 x 1.5 x 1.1 

Tip: 2 x 1.1 x 0.4 


Port #2954640) 

849 #250512863 

889 #250512753 

215, Multiple Probe 215, 

223, 232 XL/233 XL 

27367423 Flat Teflon® coated 220.5 x 0.9 232 XL/233 XL 

210 mm Z-arm 


260370011 X Beveled 


(coated) 

304 x 2 x 0.8 

Tip: 2.1 x 1.5 x 0.8 

301.3 x 2 x 0.8 

Tip: 2.1 x 1.5 x 0.8 


260370021 X Beveled 

26037003 


(coated) 

301.3 x 1.5 x 0.4 


26037005 Beveled Stainless steel 304 x 1.5 x 1.1 



#26035411) 



#26035411) 



#26035411) 



#26035411) 



#26035411) 



#26035411) 

GX-281 

GX-281 

GX-281 

GX-281 

GX-281 

GX-281 

26037006 Flat Stainless steel 304 x 2.4 x 1.8 GX-281 

26037007 Flat Stainless steel 304 x 1.8 x 1.4 GX-281 

1A 

1 

H 

1.008 

3 

Li 

6.941 

11 

Na 

23.00 

19 

K 

39.10 

37 

Rb 

85.47 

55 

Cs 

132.9 

87 

Fr 

(223) 

2A 

4 

Be 

9.012 

12 

Mg 

24.31 

20 

Ca 

40.08 

38 

Sr 

87.62 

56 

Ba 

137.3 

88 

Ra 

226.0 

3B 

21 

Sc 

44.96 

39 

Y 

88.91 

* 

** 

*Lanthanides 

**Actinides 

4B 

22 

Ti 

47.90 

40 

Zr 

91.22 

72 

Hf 

178.5 

104 

Rf 

(261) 

57 

La 

138.9 

89 

Ac 

227.0 

Periodic Table of the Elements 

5B 

23 

V 

50.94 

41 

Nb 

92.91 

73 

Ta 

180.9 

105 

Ha 

(262) 

58 

Ce 

140.1 

90 

Th 

232.0 

6B 

24 

Cr 

52.00 

42 

Mo 

95.94 

74 

W 

183.9 

106 

Unh 

(263) 

59 

Pr 

140.9 

91 

Pa 

231.0 

7B 

25 

Mn 

54.94 

43 

Tc 

(98) 

75 

Re 

186.2 

107 

Uns 

(262) 

60 

Nd 

144.2 

92 

U 

238.0 

26 

Fe 

55.85 

44 

Ru 

101.1 

76 

Os 

190.2 

108 

Hs 

(277) 

61 

Pm 

(145) 

93 

Np 

237.0 

8B 

27 

Co 

58.93 

45 

Rh 

102.9 

77 

Ir 

192.2 

62 

Sm 

150.4 

94 

Pu 

(244) 

28 

Ni 

58.70 

46 

Pd 

106.4 

78 

Pt 

195.1 

63 

Eu 

152.0 

95 

Am 

(243) 

1B 

29 

Cu 

63.55 

47 

Ag 

107.9 

79 

Au 

197.0 

64 

Gd 

157.3 

96 

Cm 

(247) 

2B 

30 

Zn 

65.38 

48 

Cd 

112.4 

80 

Hg 

200.6 

65 

Tb 

158.9 

97 

Bk 

(247) 

3A 

5 

B 

10.81 

13 

Al 

26.98 

31 

Ga 

69.72 

49 

In 

114.8 

81 

Tl 

204.4 

66 

Dy 

162.5 

98 

Cf 

(251) 

6 

C 

12.01 

14 

Si 

28.09 

32 

Ge 

72.59 

50 

Sn 

118.7 

82 

Pb 

207.2 

67 

Ho 

164.9 

99 

Es 

(252) 

7 

N 

14.01 

15 

P 

30.97 

33 

As 

74.92 

51 

Sb 

121.8 

83 

Bi 

209.0 

68 

Er 

167.3 

100 

Fm 

(257) 

8 

O 

16.00 

16 

S 

32.06 

34 

Se 

78.96 

52 

Te 

127.6 

84 

Po 

(209) 

69 

Tm 

168.9 

101 

Md 

(258) 

9 

F 

19.00 

17 

Cl 

35.45 

35 

Br 

79.90 

53 

I 

126.9 

85 

At 

(210) 

70 

Yb 

173.0 

102 

No 

(259) 

Undiscovered Synthetic From Decay Primordial 

2 

He 

4.003 

10 

Ne 

20.18 

18 

Ar 

39.95 

36 

Kr 

83.80 

54 

Xe 

131.3 

86 

Rn 

(222) 

109 

Une 

110 

Ds 

111 

Rg 

112 

Uub 

113 

Uut 

114 

Uuq 

115 

Uup 

116 

Uuh 

117 

Uus 

118 

Uuo 

Not Yet 

(276) (281) (280) (285) (284) (289) (288) (291) Observed (294) 

Flow Cell Selection Chart for Optimizing Sensitivity on 15X Series Detectors 

Selecting the correct flow cell will optimize sensitivity and peak shape. The following chart pairs flow cells with the 

appropriate column, flow rate and sample concentration to help choose the correct flow cell for the application. 

Capillary Flow Cell Micro-bore Flow Cell Analytical Flow Cell Semi-preparative Flow Cell Preparative Flow Cell 

Product # 104446 104442 104445 104441 104443 

Internal Volume 35 nL 1.5 µL 12 µL 0.7 µL 0.16 µL 

Pathlength 8 mm 2 mm 5 mm 0.2 mm 0.05 mm 

Column Size 180 – 300 µm 1 – 2 mm 4 mm 

10 mm 

4A 

5A 

6A 

7A 

20 mm > 40 mm 

8A 

71 

Lu 

175.0 

103 

Lr 

(260) 

Flow Rate 1 – 10 µL/min 25 – 200 µL/min 1 – 15 mL/min 15 – 50 mL/min 30 – 200 mL/min 

Purify ug - 5 mg Purify 5 – 300 mg Purify 300 mg - 15 g 


59

Reference 

pH of Acids and Bases 

pH of Common Acids 

pH of Common Bases 

Acids (pH < 7) Molarity pH 

Bases (pH > 7) Molarity pH 

Acetic 1 N 2.4 

Ammonia 1 N 11.6 

Acetic 0.1 N 2.9 

Ammonia 0.1 N 11.1 

Acetic 0.01 N 3.4 

Ammonia 0.01 N 10.6 

Alum 0.1 N 3.2 

Barbital Sodium 0.1 N 9.4 

Arsenious Saturated 5 

Borax 0.01 N 9.2 

Benzoic 0.1 N 3 

Calcium Carbonate Saturated 9.4 

Boric 0.1 N 5.3 

Calcium Hydroxide Saturated 12.4 

Carbonic Saturated 3.8 

Ferrous Hydroxide Saturated 9.5 

Citric 0.1 N 2.1 

Lime Saturated 12.4 

Formic 0.1 N 2.3 

Magnesia Saturated 10.5 

Hydrochloric 1 N 0.1 

Potassium Acetate 0.1 N 9.7 

Hydrochloric 0.1 N 1 

Potassium Bicarbonate 0.1 N 8.2 

Hydrochloric 0.01 N 2 

Potassium Carbonate 0.1 11.5 

Hydrocyanic 0.1 N 5.1 

Potassium Cyanide 0.1 N 11 

Hydrogen Sulfide 0.1 N 4.1 

Potassium Hydroxide 1 N 14 

Lactic 0.1 N 2.4 

Potassium Hydroxide 0.1 N 13 

Malic 0.1 N 2.2 

Potassium Hydroxide 0.01 N 12 

Nitric 0.1 N 1 

Sodium Acetate 0.1 N 8.9 

Orthophosphoric 0.1 N 1.5 

Sodium Benzoate 0.1 N 8 

Oxalic 0.1 N 1.3 

Sodium Bicarbonate 0.1 N 8.4 

Succinic 0.1 N 2.7 

Sodium Carbonate 0.1 N 11.6 

Salicylic Saturated 2.4 

Sodium Hydroxide 1 N 14 

Sulfuric 1 N 0.3 

Sodium Hydroxide 0.1 N 13 

Sulfuric 0.1 N 1.2 

Sodium Hydroxide 0.01 N 12 

Sulfuric 0.01 N 2.1 

Sodium Metasilicate 0.1 N 12.6 

Sulfurous 0.1 N 1.5 

Sodium Sesquicarbonate 0.1 N 10.1 

Tartaric 0.1 N 2 

Trisodium Phosphate 0.1 N 12 

Trichloracetic 0.1 N 1.2 

Column Sizes and Capacities 

Internal Diameter (mm) Typical Injection Volume (µL) Load Capacity (mg) Typical Flow Rates (mL/min) 

4.6 15 1 0.5 – 2 

10 100 4.7 4 – 15 

21.2 400 19.5 10 – 50 

30 1,000 42.5 40 – 100 

50 2,000 118 100 – 300 

100 10,000 473 400 – 1000 

Maximum UV Absorbance for Commonly Used Solvents 

Operating at a wavelength near or below a mobile phase solvent’s maximum UV absorbancy increases baseline noise 

due to the UV response of the solvent. Refer to the tables below to optimize chromatographic conditions. 

Solvent 

Table 1 

Maximum UV Absorbance (nm) 

1-Nitropropane 380 

2-Butoxyethanol 220 

Acetone 330 

Acetonitrile 190 

Amyl Alcohol 210 

Amyl Chloride 225 

Benzene 280 

Butanol-1 215 

Butanol-2 260 

n-Butyl Chloride 220 

Carbon Disulfide 380 

Carbon Tetrachloride 265 

Chloroform 245 

Clorobenzene 287 

Cyclohexane 200 

Cyclopentane 200 

Diethyl Amine 275 

Dimethyl Sulfoxide 268 

Dioxane 215 

Ethanol 210 

Ethyl Acetate 256 

Ethyl Ether 220 

Ethyl Sulfide 290 

Ethylene Dichloride 230 

Ethylene Glycol 210 

Heptane 200 

Hexane 195 

Isooctane 215 

Isopropanol 205 

Isopropyl Chloride 225 

Isopropyl Ether 220 

Methanol 205 

Methyl Acetate 260 

Methyl Ethyl Ketone 330 

Methyl Isobutyl Ketone 334 

Methylene Chloride 233 

n-Pentane 190 

n-Propanol 210 

n-Propyl Chloride 225 

Nitromethane 380 

Petroleum Ether 210 

Pyridine 330 

Tetrahydrofuran 230 

Toluene 285 

Xylene 290 

Mobile Phase 

Conditioners & Buffers 

Table 2 

Maximum UV 

Absorbance (nm) 

Acetic Acid, 1% 230 

Ammonium Acetate, 

10 mM 

Ammonium Bicarbonate, 

10 mM 

Diammonium Phosphate, 

50 mM 

205 

190 

205 

EDTA, Disodium, 1 mM 190 

HEPES, 10 mM, pH 7.6 225 

Hydrochloric acid, 0.1% 190 

MES, 10 mM, pH 6.0 215 

Potassium Phosphate 

Monobasic, 10 mM 

Dibasic, 10 mM 

190 

190 

Sodium Acetate, 10 mM 205 

Sodium Chloride, 1M 207 

Sodium Citrate, 10 mM 225 

Sodium Dodecyl Sulfate 190 

Sodium Formate 200 

Triethylamine, 1% 235 

Trifluoracetic Acid, 0.1% 190 

TRIS HCl, 20 mM 

pH 7.0 

pH 8.0 

202 

212 


61

Reference 

Optimizing Preparative HPLC 

There are numerous parameters and techniques to optimize preparative HPLC. These reference tables provide important 

physical parameters of the tubing and injection valve structure; optimization will improve chromatography and percent 

recovery of collected fractions. 

Table 1: 33X Series pump tubing (PEEK)* 

At high-flow rates tubing can cause 

Tubing ID (in) 

Flow Rate (mL/min) 

high back pressure. Ensuring that 

0.02 Pressure (psi) 0.03 Pressure (psi) 0.04 Pressure (psi) 

25 0 0 0 

the appropriate tubing ID/flow rate 

50 43.5 0 0 

combination is used will optimize the 

75 101.5 0 0 

pumping system. 

*30” lengths of tubing used at corresponding flow 

rates for tubing pressure. 

Tubing was connected directly to the pump. 

All pre-filters were removed. 

Product #: 25051043 (7010 valve with 

large-bore rotor seal) 

· 0.018" make-before-break stator orifice 

· 1 mL stainless steel 0.030 " ID loop 

· All other plumbing used is stainless steel 

0.040 " ID tubing 

Flow Rate 

(mL/min) 

100 203.1 14.5 0 

125 333.6 43.5 0 

150 464.1 87 14.5 

175 623.7 101.5 14.5 

200 797.7 145 29 

Table 2: Rheodyne 7010 – 156 Factory 

Installed Large-bore Rotor Seal 

Valve Position and Pressure 

Standard 7010 Bore Size 

7010 with Large-bore Rotor 

Load Position 

Pressure (psi) 

Inject Position 


Gilson Installed 

Load Position 


Gilson Installed 

Inject Position 


25 0 14.5 0 0 – 14.5 

50 0 43.5 0 43.5 

75 0 87 0 116 

100 14.5 145 14.5 203.1 

150 43.5 290 72.5 435.1 

200 87 435.1 145 696.2 

Solvent Miscibility and Polarity 

Xylene 

Water 

Tichloroethylene 

Toluene 

Tetrahydrofuran 

Di-Iso-Propyl Ether 

Iso-Propanol 6 

n-Propanol 

Pentane 

Solvent Miscibility Table 

Methyl Ethyl Ketone 5 

Methyl-t-Butyl Ether 4 

Methanol 

Hexane 

Heptane 

Di-Ethyl Ether 

Ethyl Acetate 

Ethanol 

Dioxane 

Dimethyl Sulfoxide 3 

Dimethylformamide 

Dichloromethane 2 

1,2-Dichloroethane 1 

Cyclohexane 

Chloroform 

Carbon Tetrachloride 

Butyl Acetate 

n-Butanol 

Benzene 

Acetonitrile 

Acetone 

Acetic Acid 

Solvent 

Polarity 

Index 

Refraction 

Index 

@20°C 

UV(nm) 

Cut-off 

@1AU 

Boiling 

Point 

(°C) 

Viscosity 

(cPoise) 

Solubility 

in water 

(%w/w) 

Acetic Acid 6.2 1.372 230 118 1.26 100 

Acetone 5.1 1.359 330 56 .032 100 

Acetonitrile 5.8 1.344 190 82 0.37 100 

Benzene 2.7 1.501 280 80 0.65 0.18 

n-Butanol 4.0 1.394 254 125 0.73 0.43 

Butyl Acetate 3.9 1.399 215 118 2.98 7.81 

Carbon Tetrachloride 1.6 1.466 263 77 0.97 0.08 

Chloroform 4.1 1.446 245 61 0.57 0.815 

Cyclohexane 0.2 1.426 200 81 1.00 0.01 

1,2-Dichloroethane 1 3.5 1.444 225 84 0.79 0.81 

Dichloromethane 2 3.1 1.424 235 41 0.44 1.6 

Dimethylformamide 6.4 1.431 268 155 0.92 100 

Dimethyl Sulfoxide 3 7.2 1.478 268 189 2.00 100 

Dioxane 4.8 1.422 215 101 1.54 100 

Ethanol 5.2 1.360 210 78 1.20 100 

Ethyl Acetate 4.4 1.372 260 77 0.45 8.7 

Di-Ethyl Ether 2.8 1.353 220 35 0.32 6.89 

Heptane 0.0 1.387 200 98 0.39 0.0003 

Hexane 0.0 1.375 200 69 0.33 0.001 

Methanol 5.1 1.329 205 65 0.60 100 

Methyl-t-Butyl Ether 4 2.5 1.369 210 55 0.27 4.8 

Methyl Ethyl Ketone 5 4.7 1.379 329 80 0.45 24 

Pentane 0.0 1.358 200 36 .23 .004 

n-Propanol 4.0 1.384 210 97 2.27 100 

Iso-Propanol 6 3.9 1.377 210 82 2.30 100 

Di-Iso-Propyl Ether 2.2 1.368 220 68 0.37 

Tetrahydrofuran 4.0 1.407 215 65 0.55 100 

Toluene 2.4 1.496 285 111 0.59 0.051 

Tichloroethylene 1.0 1.477 273 87 0.57 0.11 

Water 9.0 1.333 200 100 1.00 100 

Xylene 2.5 1.500 290 139 0.61 0.018 

Immiscible 

Miscible 

Immiscible means that in some proportions 

two phases will be produced. 

Synonym Table 

1 

Ethylene Chloride 

2 

Methylene Chloride 

3 

Methyl Sulfoxide 

4 

tert-Butyl Methyl Ether 

5 

2-Butanone 

6 

2-Propanol 

Separation Scale-up Equations 

Scale-up Factor 

(diameter preparative) 2 

scale-up factor = __________________________ 

(diameter analytical) 2 

Flow Rate 

flow rate preparative = 

scale-up factor 

__________________________ 

analytical flow rate 

Gradient Duration (GD) 

length preparative (diameter preparative) 2 flow rate analytical 

GD preparative = GD analytical x __________ x _____________ x ____________ 

length analytical (diameter analytical) 2 flow rate preparative 

Relative 

Polarity 

Compound 

Formula 

Solvent Polarity Chart 

Group 

Representative Solvent Compounds 

Non-polar R - H Alkanes Petroleum Ethers, Ligroin, Hexanes 

Ar - H Aromatics Toluene, Benzene 

R - O - R Ethers Diethyl Ether 

R - X Alkyl Halides Tetrachloromethane, Chloroform 

R - COOR Esters Ethyl Acetate 

R - CO - R Aldehydes and Ketones Acetone, Methyl Ethyl, Ketone 

R - NH 2 Amines Pyridine, Triethylamine 

R - OH Alcohols Methanol, Ethanol, Isopropanol, Butanol 

R - COHN 2 Amides Dimethylformamide 

R - COOH Carboxylic Acids Ethanoic Acid 

Polar H - OH Water Water 

Increasing Polarity 


63

Reference 

Common Conversion Tables 

Temperature Conversion 

Fractional inch-to-decimal-to-millimeter Conversion 

Tubing Internal Diameters & Volumes 

Pressure Conversion 

Celsius (°C) 

Fahrenheit (°F) 

-100 -148 

-50 -58 

-40 -40 

-30 -22 

-20 -4 

-10 14 

0 32 

5 41 

10 50 

15 59 

20 68 

25 77 

30 86 

35 95 

40 104 

45 113 

50 122 

55 131 

60 140 

65 149 

70 158 

75 167 

80 176 

85 185 

90 194 

95 203 

100 212 

105 221 

110 230 

115 239 

120 248 

125 257 

130 266 

135 275 

140 284 

145 293 

150 302 

155 311 

160 320 

165 329 

170 338 

175 347 

180 356 

185 365 

190 374 

195 383 

200 392 

Fraction (") Decimal Millimeter 

1/32 .0313 .7938 

1/16 .0625 1.5875 

3/32 .0938 2.3813 

1/8 .125 3.175 

5/32 .1563 3.9688 

3/16 .1875 4.7625 

7/32 .2188 5.5563 

1/4 .25 6.35 

9/32 .2813 7.1438 

5/16 .3125 7.9375 

11/32 .3438 8.7313 

3/8 .375 9.525 

13/32 .4063 10.3188 

7/16 .4375 11.1125 

15/32 .4688 11.9063 

1/2 .5 12.7 

17/32 .5313 13.4938 

9/16 .5625 14.2875 

19/32 .5938 15.0813 

5/8 .625 15.875 

21/32 .6563 16.6688 

11/16 .6875 17.4625 

23/32 .7188 18.2563 

3/4 .75 19.05 

25/32 .7813 19.8438 

13/16 .8125 20.6375 

27/32 .8438 21.4313 

7/8 .875 22.225 

29/32 .9063 23.0188 

15/16 .9375 23.8125 

31/32 .9688 24.6063 

1 1 25.4 

Inch Millimeter Micron µL/in µL/mm 

0.001 0.025 25 0.013 0.0005 

0.002 0.051 51 0.051 0.002 

0.003 0.076 76 0.116 0.0046 

0.004 0.102 102 0.206 0.0081 

0.005 0.127 127 0.322 0.0127 

0.006 0.152 152 0.463 0.0182 

0.007 0.178 178 0.631 0.0248 

0.008 0.203 203 0.824 0.0324 

0.009 0.229 229 1.042 0.041 

0.01 0.254 254 1.287 0.0507 

0.011 0.279 279 1.557 0.0613 

0.012 0.305 305 1.853 0.073 

0.013 0.33 330 2.175 0.0856 

0.014 0.356 356 2.523 0.0993 

0.015 0.381 381 2.896 0.114 

0.016 0.406 406 3.295 0.1297 

0.017 0.432 432 3.72 0.1464 

0.018 0.457 457 4.17 0.1642 

0.019 0.483 483 4.646 0.1829 

0.02 0.508 508 5.148 0.2027 

0.028 0.711 711 10.09 0.3973 

0.03 0.762 762 11.583 0.456 

0.032 0.813 813 13.179 0.5189 

0.04 1.016 1016 20.593 0.8107 

0.042 1.067 1067 22.703 0.8938 

0.055 1.397 1397 38.933 1.5328 

0.062 1.575 1575 49.474 1.9478 

Temperature Conversion Formulas 

˚C = ˚F – 32 ÷ 1.8 

˚C = ˚K – 273.15 

˚F = 1.8 x ˚C + 32 

˚F = ˚R – 459.67 

˚K = ˚C + 273.15 

˚R = ˚F + 459.67 

˚C = degrees Celsius 

˚F = degrees Fahrenheit 

˚K = degrees Kelvin 

˚R = degrees Rankine 

kPa psi bar MPa 

690 100 6.9 0.7 

3450 500 34.5 3.4 

6890 1000 68.9 6.9 

10340 1500 103.4 10.3 

13790 2000 137.9 13.8 

17240 2500 172.4 17.2 

20680 3000 206.8 20.7 

24130 3500 241.3 24.1 

27580 4000 275.8 27.6 

31030 4500 310.3 31 

34470 5000 344.7 34.5 

37920 5500 379.2 37.9 

41370 6000 413.7 41.4 

44820 6500 448.2 44.8 

48260 7000 482.6 48.3 

51710 7500 517.1 51.7 

55160 8000 551.6 55.2 

58610 8500 586.1 58.6 

62050 9000 620.5 62.1 

65500 9500 655 65.5 

68950 10000 689.5 68.9 

Metric Prefixes 

Prefix Symbol Scientific Notation 

yotta Y 10 24 

zetta Z 10 21 

exa E 10 18 

peta P 10 15 

tera T 10 12 

giga G 10 9 

mega M 10 6 

kilo k 10 3 

hecto h 10 2 

deka da 10 

deci d 10 -1 

centi c 10 -2 

milli m 10 -3 

micro µ 10 -6 

nano n 10 -9 

pico p 10 -12 

femto f 10 -15 

atto a 10 -18 

zepto z 10 -21 

yocto y 10 -24 


65

Reference 

Gilson Applications 

Liquid Chromatography 

Liquid Chromatography continued 

cLC System: An Automated Solution for Normal and Reversedphase 

Preparative HPLC with Analytical Purification Determination 

The cLC system offers researchers the capabilities of both RP 

and NP chromatography on a single system, including data 

representing the throughput and analysis capabilities of 

the system. 

Optimizing Purified Sample Recovery on Reversed-phase 

HPLC Systems 

This application presents the factors that can directly affect 

purity and recovery when processing compounds on a 

preparative level. The following system parameters were 

evaluated: collection valve switch time, detector time constant 

delay, DMSO sandwich solvent, delay volume from detector to 

collection valve and software response. 

A Totally Integrated 2D System Incorporating Ion-exchange and 

RP-HPLC for Natural and Biological Products 

An automated two-dimensional application that incorporates 

ion-exchange and reversed-phase chromatography for the 

separation of complex mixtures. Data is presented that shows 

the usefulness of two dimensional chromatography and 

implementing alternative chromatography modes. 

Preparative HPLC: Factors and Parameters that Directly Affect 

Recovery of Collected Fractions 

Parameters discussed are column performance, mobile phase 

constituents, detector settings and collection parameters 

such as collection via level versus slope and delay volumes. The 

data offers guidelines for all chromatographers purifying 

compounds by preparative HPLC and can be implemented into 

existing systems. 

Separation of Flavonoids Through Automated Multiple Column 

Injections and Sample Concentration via C18 Column Capture 

Identical columns are configured in parallel for multiple injections 

sequenced by the sample peak elution. Sample is concentrated 

on a separate C18 preparative column, the purified compound is 

eluted in high organic solvent for ease of dry-down. 

Enhanced Recovery of Compound Mixtures by Concurrent 

Dissection and Concentration of Isolates 

A crude separation of a complex mixture into impure isolates. 

The impure isolates are reintroduced to the column. Front 

and back sections are cleaved to fractionate uncontaminated 

compound on separate columns for concentration. Final 

elution dispenses pure compound in high organic solvent for 

ease of dry-down. 

Automated Post-collection Concentration for Purified Preparative 

Fractions via SPE 

The method (as opposed to analytical applications involving 

SPE) works by collecting and then diluting the fraction(s), 

passing the fraction(s) through an SPE and ultimately 

eluting the concentrated fraction in a small amount of 

100% organic solvent. 

Determination of Reproducibility within an Analytical System via 

Repeat Injections 

The data presented in this application shows that the 

Gilson HPLC system provides consistent and reproducible 

chromatography. A mixture of three compounds was injected 

repeatedly under analytical reversed-phase conditions. 

Novel Approach for Screening of Drug Absorption via an 

Automated System 

The system presented provides a faster alternative to other 

screening methods, as well as an inexpensive analysis estimate 

of drug permeability for hundreds of drug candidates in a 

fraction of the time of conventional screening methods. 

A Multi-purpose Preparative System for the Detection and 

Collection of Active and Non-active UV/VIS Compounds 

The process relies on an analytical scout analysis to determine 

the best gradient conditions to optimize the separation of the 

compounds under preparative conditions and minimize the 

number of fractions collected per run by incorporating ELS 

detection in addition to UV/VIS detection. 

Systematic Look at the Affects of Various Solvents, Injection 

Techniques and Sample Amounts in Preparative HPLC Columns 

Data is presented documenting the affects elicited in 

preparative chromatography under various conditions. 

Optimized Sample Load Capacity and Separation Through a 

Series of Short Preparative Columns 

By placing the smaller preparative columns in series, loading 

capacity and separations of compounds can increase with 

short run times and semi-preparative flow rates, minimizing the 

overall cost of the larger preparative columns and channeling 

within the column. 

“Train Keep a Rolling and Isolation Gradients”: A Method for 

Purifying a Large Quantity of Sample via Continuous Injection and 

Simultaneous Fraction Collection on Multiple FC Beds 

Train injection employs continuous injection of sample under 

isocratic conditions and continuous fraction collection. In 

addition, isolation gradients also offer an efficient purification 

of preparative samples when the train injection technique is 

not applicable, as is the case with complicated chromatograms. 

Optimizing Chiral Separations via an Automated Parallel Scouting 

System for On-line Preparative Chiral Purification 

The system is capable of screening chiral compounds on four 

separate columns in parallel, increasing the stereoselective 

screening by four fold. 

Technical Data for the Quad-Z 215 (fixed probes) Dispensing Small 

Volumes of DMSO 

This study determined the accuracy and CVs for the Quad-Z 

215 (fixed probes) for low volume dispensing of DMSO. The 

results of the testing show the Quad-Z 215 is capable of 

transferring low volumes of DMSO from a 96-well format into 

an empty, flat-bottomed 96-well microplate. 

Quad-Z 215 with Disposable Tips: Implementing the Use of 

ZipTips® for the Purification of Biological Samples 

This application demonstrates how the Quad-Z 215 automates 

sample clean-up using ZipTips. The Quad-Z 215 is also capable 

of completing numerous liquid handling techniques – with or 

without tips – under varying microplate formats. 

Reformatting Procedures Employing a Quad-Z 215 with 

Disposable Tips 

The volumetric accuracy and precision data presented show 

that the Quad-Z 215 with disposable tips offers researchers 

the consistency they require in all assays. The use of common 

tubes and microplates, along with the option of designing 

a custom rack, provides a high degree of versatility to 

accommodate variations in procedures. 

Automated Liquid Handling 

Accessing the E-Gel® 96 System via the Quad-Z 215 with 

Disposable Tips 

This application examines the suitability of the Quad-Z 215 

with disposable tips for the delivery of proteins to the wells of 

the E-Gel 96 gels for electrophoresis. 

A Compact Automated Liquid Handler for Microbatch Protein 

Crystallography 

A system that incorporates fixed and disposable tips on the 

Quad-Z 215. Independent probe motion and the use of virtual 

pumps make the system very versatile in the field of protein 

crystallography via microbatch techniques. 


67

Reference 

Gilson Applications continued 

An Automated SPE Procedure and Analysis for the Extraction of 

Chlorpromazine and Thioridazine from Plasma 

This application describes an automated SPE method coupled 

with HPLC analysis to determine the levels of Chlorpromazine 

and Thioridazine in human plasma. 

An Automated SPE Procedure and Analysis for the Determination 

of Ibuprofen and Ketoprofen in Plasma 

This application describes an automated SPE method coupled 

with HPLC analysis to determine the various levels of Ibuprofen 

and Ketoprofen in human plasma. 

The Ephedra Story: An Automated SPE Procedure and Analysis for 

the Determination of Ephedra and Ephedra-like Compounds in 

Dietary Supplements 

This application presents the advantages of using an 

automated SPE method coupled with HPLC, versus manual 

SPE, to determine the various levels of Ephedra and Ephedralike 

compounds in dietary supplements. 

Optimization of SPE by an Automated Method Development 

Procedure 

The system allows for the optimization of SPE process via 

multiple collection sites and various conditions without 

manual intervention. Incorporation of a trace enrichment 

cartridge to concentrate the eluent versus a dry-down step 

offers advantages to the dry-down bottle neck and analyte 

degradation. 

Solid Phase Extraction 

Automated Post-collection Concentration for Purified Preparative 

Fractions via SPE 

The method (as opposed to analytical applications involving 

SPE) works by collecting and then diluting the fraction(s), 

passing the fraction(s) through an SPE and ultimately eluting 

the concentrated fraction in a small amount of 100% organic 

solvent. 

Isolation and Determination of Capsaicin and Dihydrocapsaicin 

from Complex Matrices via Automated SPE and UV/ELS/MS 

Detection 

Matrices tested include dried habanero, topical analgesic 

cream and pepper spray. The system allows for the extraction 

procedure to be automatically optimized for each matrix 

without operator intervention, providing high recovery. 

Flash: Eliminating the Concentration/Dry-down Required for a 

Purified Compound by Preparative Chromatography 

Employing an activated media packed in an SPE/Flash 

cartridge (60 mL/10 gm), the automated system can 

intelligently identify the purified fractions and compound 

mass 0.1 – 2 g, without time-consuming dry-down. 

Totally Automated Method for the Determination of Sudan Dyes in 

Food via On-line Filtration, SPE and HPLC Analysis 

The system presented offers a totally automated approach to 

the extraction of Sudan dyes from food products. The product 

is solubilized, filtered and SPE is performed prior to HPLC 

analysis, at detection limits below the EU of 0.5 – 1 mg/kg. 

Quality Policy 

It is Gilson, Inc.’s policy to promote a total commitment to quality by each individual throughout the 

Gilson organization worldwide. It is Gilson’s goal that this commitment to quality will result in 

complete customer satisfaction with both Gilson’s products and services. 

ISO 9001 Certification 

Gilson is committed to quality, and it shows in everything Gilson does – from design to manufacturing, 

technical support and training. Due to this commitment to quality and customer satisfaction, Gilson 

has consistently been awarded with ISO 9001 certification. Gilson, Inc. and Gilson S.A.S. are both 

ISO 9001 certified. 

Training 

Gilson provides an extensive program of instrument and software training for users. Standard courses as 

well as sessions customized to the user’s specific needs are offered at Gilson’s innovative training center or 

in the user’s own laboratory. Computer assisted training courses are also available on CD-ROM or the Gilson 

on-line education center, www.training.gilson.com. 

Gilson, Inc. World Headquarters 

3000 Parmenter Street | P.O. Box 620027 | Middleton, WI 53562-0027, USA 

Tel: 608-836-1551 or 800-445-7661 | Fax: 608-831-4451 

sales@gilson.com | service@gilson.com | training@gilson.com | hr@gilson.com | oem@gilson.com 

A Rapid Extraction and Clean-up Procedure for the Determination 

of Amnesic Shellfish Poison Toxins in Shellfish 

A rapid sample preparation procedure has been developed 

via SPE to determine the levels of domoic acid in shellfish. 

Data supporting this approach is presented along with the 

automated SPE procedure incorporating trace enrichment 

cartridges to increase sensitivity of the assay. 

Optimizing Parameters that Influence Recovery in SPE 

A totally automated approach to optimize every aspect 

of an SPE method without manual intervention on an SPE 

instrument via fractionation, improving recovery and reducing 

coefficient of variance. 

Gilson S.A.S. 

19, avenue des Entrepreneurs | BP 145, F-95400 VILLIERS LE BEL, FRANCE 

Tel: 33-(0)1-34295000 | Fax: 33-(0)1-34295020

Gilson, Inc. World Headquarters | 3000 Parmenter Street | P.O. Box 620027 | Middleton, WI 53562-0027, USA | Tel: 608-836-1551 or 800-445-7661 | Fax: 608-831-4451 

Gilson S.A.S. | 19, avenue des Entrepreneurs | BP 145, F-95400 VILLIERS LE BEL, FRANCE 

www.gilson.com | sales@gilson.com | service@gilson.com | training@gilson.com 

©2007 - 2008 Gilson, Inc. | LT303041-09 | Specifications subject to change without notice.

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