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Protein

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Protein

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Dissociation constants<br />

Normalized wash intensity above background<br />

0.9<br />

0.8<br />

0.7<br />

0.6<br />

0.5<br />

0.4<br />

0.3<br />

0.2<br />

0.1<br />

0<br />

10 0 10 1 10 2 10 3<br />

[GCN4] (nM)<br />

Hill coefficient = 2.0<br />

KD = 42 nM

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