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Table A5. Pathogen and treatment combinations used in Trial 6
Treatment # Isolate Treatment Concentration/20g
seed
1 Nil Nil 1 g Gypsum+ 1 ml H 2 0
2 Rhizoctonia 04/793 Nil 1 g Gypsum+ 1 ml H 2 0
3 Pythium 05/986 Nil 1 g Gypsum+ 1 ml H 2 0
4 Phytophthora 05/1011 Nil 1 g Gypsum+ 1 ml H 2 0
5 Rhizoctonia 04/793 Dynasty ® CST +
Tecto®
100 ul+286ul + 614 ul
H 2 0
6 Nil Dynasty® CST + 100 ul+286 ul + 614 ul
Tecto®
7 Rhizoctonia 04/793 Dynasty ® CST +
Tecto® + Bion
500SC
8 Nil Dynasty® CST +
Tecto® + Bion
500SC
H 2 0
100 ul+286 ul + 12 ul +
602 ul H 2 0
100 ul+286 ul + 12 ul +
602 ul H 2 0
9 Rhizoctonia 04/793 Tecto® 286 ul + 714 ul H 2 0
10 Nil Tecto® 286 ul + 714 ul H 2 0
11 Pythium 05/986 Dynasty ® CST 100 ul / 20 g seed + 200
ul H 2 0
12 Phytophthora 05/1011 Dynasty® CST 100 ul +900 ul H 2 0
13 Nil Dynasty® CST 100 ul +900 ul H 2 0
14 Pythium 05/986 Dynasty® CST +
Bion 500SC
100 ul + 12 ul + 878 ul
H 2 0
15 Phytophthora 05/1011 Dynasty ® CST +
Bion 500SC
100 ul + 12 ul + 878 ul
H 2 0
16 Rhizoctonia 04/793 Dynasty ® CST +
Bion 500SC
100 ul + 12 ul + 878 ul
H 2 0
17 Nil Dynasty® CST +
Bion 500SC
100 ul + 12 ul + 878 ul
H 2 0
18 Pythium 05/986 Bion 500SC 12 ul +988 ul H 2 0
19 Phytophthora 05/1011 Bion 500SC 12 ul +988 ul H 2 0
20 Rhizoctonia 04/793 Bion 500SC 12 ul +988 ul H 2 0
21 Nil Bion 500SC 12 ul +988 ul H 2 0
2.3.6 Trial 7 Chemical seed dressings for control of root rot pathogens of English
spinach
The isolates Pythium 05/986, Phytophthora 05/986 and Rhizoctonia 04/793 were grown on the
selective agar Potato carrot agar (PCA) for 12 days at 25 o C. These were homogenised and diluted in
distilled water and made up to volume, 200 ml was added per pot (one plate per 3 L pot). This was
incorporated into the top 3 cm of medium, consisting of peat, perlite and sand at a 1:1:1 ratio (pH 6.3
and EC 450ms). Uncolonised and homogenised PCA was used as Nil pathogen treatments. A pool
solution of the fungicides was made and volumes added to individual treatments. The seed was treated
and allowed to air-dry overnight in a fume hood. It was sown the next day. A total of 50 seeds were
uniformly sown in each pot. There were 16 treatments, which were replicated five times. Details of
each treatment are presented below in Table A6.
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