Antimicrobial Test Report - Drapilux

Antimicrobial Test Report
Customer: Schmitz via: BST
Report date: 03. September 2010
Report number: BT10-0102 Internal reference: TS10-0121
Short Description: 2-step application: Curtain fabric treated with Pure by HeiQ ® and Air by Schmitz.
Testing antimicrobial activity against MRSA.
1 Sample Description
Sample Details: Curtain fabric, PET Trevira CS, mesh like
Sample Preparation: Application by customer Application by HeiQ
Mill trial
Laboratory
Treatment:
HeiQ AGS-20 TF (antimicrobial textile finish)
Standard formulation ‡
HeiQ AGS-20 TF: g/L
Superior Durability formulation ‡
HeiQ AGS-20 TF: 30 g/L
HeiQ AGS-20 SD: 15 g/L
at pick-up 30 %
‡ Refer to HeiQ AGS-20 TF Technical Datasheet for further details
Handling:
HeiQ AGS-20 MB (antimicrobial masterbatch)
Silver dosing range: up to ppm Ag
HeiQ AGS-20 powder
Loading range: up to % HeiQ AGS-20 (up to ppm Ag)
Other:
2. process step after Pure: Air product 120 g/l, PU-DM 10 g/l,
AGS-20 SD 10 g/l
Tested as received
Laundry testing (ISO 6330). Up to x at °C.
Other:
2 Method
Method Type:
ISO 20743 / JIS L 1902 (Textiles and absorbent articles)
ISO 22196 / JIS Z 2801 (Plastics, coatings and non-absorbent articles)
Other:
Test Organisms: Klebsiella pneumoniae (ATCC 4352)
Escherichia coli (ATCC 8739)
Staphylococcus aureus (ATCC 6538P)
Other: Methicillin-resistant Staphylococcus aureus (NCTC 8325 mec)
3 Notes
See appendix for details of test method and organisms
• When reviewing the following results tables please note the following:
o “cfu” is an abbreviation for colony forming units.
o The detection limit for bacteria count is 9.9 x10 1 cfu.
o Numbers in table are given in scientific notation (eg. 2.4E+05 indicates 2.4 x10 5 ).
Continued overleaf.
CONFIDENTIAL
Zürcherstrasse 42, CH-5330 Bad Zurzach, Switzerland Tel: +41(0)56 250 68 50 Fax: +41 (0)56 250 68 51 1/3

4 Results
Reference Material(s): HeiQ reference polyester (0912)
HeiQ reference cotton (0994)
As listed in results table(s)
MRSA (Staphylococcus aureus, NCTC 8325 mec)
# ID Sample
Bacteria count (cfu)
Mean
St.Dev
%
reduction
Log
reduction Performance
- 0912 HeiQ reference polyester (0 hrs) 7.2E+04 1.1E+04 - - -
- 0912 HeiQ reference polyester 4.3E+06 8.5E+05 - - -
1 BT10-0102-001 Treated curtain fabric, unwashed 9.9E+01 0.0E+00 99.998% 4.6 Very good
5 Test Comments
5.1 Control validity • Controls OK. No inherent antimicrobial activity.
5.2 Sample comments • No comments.
6 Remarks
• Treated curtain fabric shows very good antimicrobial activity against MRSA.
Dr. Rainer Lehmann
Institute of Food Science and Nutrition
Swiss Federal Institute of Technology (ETH Zürich)
Mr. Martin Meyer
Customer Service
HeiQ Materials
Dr. Murray Height
Chief Technology Officer
HeiQ Materials
HeiQ Materials AG
Zürcherstrasse 42
CH-5330 Bad Zurzach, Switzerland
Office +41 (0)56 250 68 50
Fax +41 (0)56 250 68 51
info@heiq.com
www.heiqmaterials.com
CONFIDENTIAL
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APPENDIX: Antimicrobial Test Methods:
1 General
• HeiQ offers a range of microbiological test services through our partner laboratory at the Swiss Federal Institute of Technology (ETH Zürich).
• The intended purpose of the test service is to provide rapid and low-cost performance feedback to aid customer product development.
• For final certification of product antimicrobial performance HeiQ can put the customer in touch with suitable independent laboratories that can
provide this service.
2 Quantitative Tests
• Quantitative test methods are used routinely for evaluating the performance of product treated with antimicrobial agents and there are various
standard methods available for this purpose.
• Each quantitative method involves the following basic steps:
1. Sterilize the sample(s) to remove any ambient organisms or contaminants.
2. Apply a known number of a target bacteria strain to each sample.
3. Maintain sample(s) at temperature to allow the bacteria to grow.
4. After a fixed time, extract and count the bacteria remaining on each sample.
5. Compare the remaining bacteria between each treated sample and an un-treated control to assess the antimicrobial performance
resulting from the treatment.
2.1 Absorbant Samples (eg. textiles, fabrics, fibers, nonwovens etc.)
• Method based on Japanese Industrial Standard JIS L 1902.
• Prepare sample test pieces of ca.0.4g, repeated in triplicate
• Samples sterilized either with steam treatment (steam @100°C for 5 minutes, drying @70°C for max. 5 hours) or 70% ethanol solution rinse.
• Prepare bacteria culture of ca. 1 x10 6 cells/ml
o Typically only Klebsiella pneumoniae is used in a first test series.
• 200 µl of bacteria culture applied to sample
• Sample incubated for 18 hours at 37 °C
• Living bacteria remaining on each sample are rinsed off and counted. Counting provides the number of so-called Colony Forming Units (cfu)
which is an indicator of bacteria population.
• Calculation of antimicrobial performance metrics (From cfu counts after 18 hrs). See metric calculations below.
2.2 Non-Absorbant Samples (eg. plastics, films, painted surfaces etc.)
• Method based on Japanese Industrial Standard JIS Z 2801.
• Sample geometry 5cm x 5cm (or equivalent area).
• Samples sterilized with 70% ethanol solution.
• Prepare bacteria culture of ca. 10 6 cells/ml.
o Typically only Escherichia coli is used in a first test series.
• 400 µl of bacteria culture applied to sample surface.
• Clear plastic sheet 4cm x 4cm (or equivalent area) applied to spread inoculum volume over sample surface.
• Sample incubated for 24 hours at 37 °C in a humidity chamber.
• Living bacteria remaining on each sample are rinsed off and counted. Counting provides the number of so-called Colony Forming Units (cfu)
which is an indicator of bacteria population.
• Calculation of antimicrobial performance metrics (From cfu counts after 24 hrs). See metric calculations below.
3 Antimicrobial Performance Metrics
• Antimicrobial performance metrics calculated from the mean cfu counts as follows.
% Reduction =
[(Mean cfu) control - (Mean cfu) sample]
(Mean cfu) control
Log reduction = Log 10(Mean cfu) control - Log 10(Mean cfu) sample
• HeiQ assigns antimicrobial performance categories according to the following table.
4 Bacteria Strains
x 100
Antimicrobial performance Log reduction % Reduction
None