16.04.2014 Views

An Approach to the Mystery of Water Based on Grander® Technology

An Approach to the Mystery of Water Based on Grander® Technology

An Approach to the Mystery of Water Based on Grander® Technology

SHOW MORE
SHOW LESS

You also want an ePaper? Increase the reach of your titles

YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.

<str<strong>on</strong>g>An</str<strong>on</strong>g> <str<strong>on</strong>g>Approach</str<strong>on</strong>g> <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> <str<strong>on</strong>g>Mystery</str<strong>on</strong>g> <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>Water</str<strong>on</strong>g><br />

<str<strong>on</strong>g>Based</str<strong>on</strong>g> <strong>on</strong> Grander ® <strong>Technology</strong><br />

<str<strong>on</strong>g>An</str<strong>on</strong>g> Interim Report by Dr. Horst Felsch<br />

© message (1), Dr. Horst Felsch (5)


C<strong>on</strong>tents<br />

4<br />

Microbiological Effects <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ® <strong>Technology</strong><br />

Pin Point Formssati<strong>on</strong><br />

11<br />

Biological Method for Proving <str<strong>on</strong>g>the</str<strong>on</strong>g> Positive Transformati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> Heavy Metal<br />

Informati<strong>on</strong> in <str<strong>on</strong>g>Water</str<strong>on</strong>g>, Using Grander ® Revitalisati<strong>on</strong><br />

The Phosphorescent Bacteria Test<br />

18<br />

Grander ® <strong>Technology</strong> Improves <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

The Preservati<strong>on</strong> Qualities <str<strong>on</strong>g>of</str<strong>on</strong>g> Drinking <str<strong>on</strong>g>Water</str<strong>on</strong>g><br />

21<br />

How L<strong>on</strong>g Is Original Grander ® Revitalisati<strong>on</strong> Equipment Effective?<br />

The Active Life-Span <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ® Revitalisers<br />

Published by: Uranus Verlagsges.m.b.H., Lange Gasse 48/5, A-1080 Vienna<br />

Tel.: 0043(0)1 403 91 11, fax: 0043(0)1 403 91 11 33, e-mail:uranus@uranus.at<br />

Resp<strong>on</strong>sible for <str<strong>on</strong>g>the</str<strong>on</strong>g> c<strong>on</strong>tents: Dr. Horst Felsch, Academically Qualified Engineer, Schlossberg 25, A-6391 Fieberbrunn<br />

Tel.: 0043(0)5354 56 050, fax: 0043(0)5354 52 248, e-mail:h.felsch@tirol.com<br />

Producti<strong>on</strong>: message Medien & VerlagsGmbH, Diefenbachgasse 5, A-1150 Vienna<br />

Litho: Brüll & Schor, Pröllgasse 4, A-1130 Vienna<br />

Printing: Druckerei Berger, A-3580 Horn<br />

Copyright: All articles ©2000, Uranus Verlagsges.m.b.H.<br />

May be reprinted <strong>on</strong>ly with express permissi<strong>on</strong><br />

2


Preface<br />

“<str<strong>on</strong>g>Water</str<strong>on</strong>g> is a cosmic thing!” That was <strong>on</strong>e <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> things Johann Grander pressed up<strong>on</strong> me<br />

in 1993, when he entrusted me with <str<strong>on</strong>g>the</str<strong>on</strong>g> task<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> testing his discovery with scientifically<br />

recognised methods. This resp<strong>on</strong>sibility was,<br />

for me, <str<strong>on</strong>g>the</str<strong>on</strong>g> beginning <str<strong>on</strong>g>of</str<strong>on</strong>g> a very exciting period<br />

and for <str<strong>on</strong>g>the</str<strong>on</strong>g> first time I had <str<strong>on</strong>g>the</str<strong>on</strong>g> feeling<br />

that I was drawing closer <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> phenomen<strong>on</strong><br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> “water and life”. On <str<strong>on</strong>g>the</str<strong>on</strong>g> o<str<strong>on</strong>g>the</str<strong>on</strong>g>r hand,<br />

I was very sceptical, because <str<strong>on</strong>g>the</str<strong>on</strong>g> relati<strong>on</strong>ship<br />

between “water and <str<strong>on</strong>g>the</str<strong>on</strong>g> cosmos” meant<br />

little <str<strong>on</strong>g>to</str<strong>on</strong>g> me.<br />

The work got underway with a series <str<strong>on</strong>g>of</str<strong>on</strong>g> labora<str<strong>on</strong>g>to</str<strong>on</strong>g>ry tests,<br />

with <str<strong>on</strong>g>the</str<strong>on</strong>g> involvement <str<strong>on</strong>g>of</str<strong>on</strong>g> Austrian university institutes. As a<br />

matter <str<strong>on</strong>g>of</str<strong>on</strong>g> principal, <str<strong>on</strong>g>the</str<strong>on</strong>g> practical implementati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ®<br />

<strong>Technology</strong> in <str<strong>on</strong>g>the</str<strong>on</strong>g> industrial field was undertaken <strong>on</strong>ly after<br />

water analysis had clearly defined <str<strong>on</strong>g>the</str<strong>on</strong>g> initial c<strong>on</strong>diti<strong>on</strong>s. After<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> equipment was installed, chemical and bacteriological tests<br />

documented any changes. This resulted in an abundance <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

data, which dem<strong>on</strong>strated <str<strong>on</strong>g>the</str<strong>on</strong>g> following: <str<strong>on</strong>g>the</str<strong>on</strong>g> use <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ®<br />

<strong>Technology</strong> results in a lower demand for chemicals, <str<strong>on</strong>g>the</str<strong>on</strong>g> bacteriological<br />

quality <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> water improves, and drinking water<br />

is preserved for years.<br />

So much for <str<strong>on</strong>g>the</str<strong>on</strong>g> technical applicati<strong>on</strong>s. But what about <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

scientific side <str<strong>on</strong>g>of</str<strong>on</strong>g> things?<br />

Occasi<strong>on</strong>ally, during discussi<strong>on</strong>s I was c<strong>on</strong>fr<strong>on</strong>ted with <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

argument: Grander ® <strong>Technology</strong> may well be effective, but no<br />

<strong>on</strong>e really understands <str<strong>on</strong>g>the</str<strong>on</strong>g> principal <str<strong>on</strong>g>of</str<strong>on</strong>g> how it works, and ultimately<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> whole matter simply has <str<strong>on</strong>g>to</str<strong>on</strong>g> be accepted <strong>on</strong> faith.<br />

In March 2000, Pr<str<strong>on</strong>g>of</str<strong>on</strong>g>essor Rachmanin and his colleague Pr<str<strong>on</strong>g>of</str<strong>on</strong>g>essor<br />

K<strong>on</strong>dra<str<strong>on</strong>g>to</str<strong>on</strong>g>v came <str<strong>on</strong>g>to</str<strong>on</strong>g> Austria in order <str<strong>on</strong>g>to</str<strong>on</strong>g> report pers<strong>on</strong>ally<br />

<strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g>ir findings at Jochberg in Tirol. <str<strong>on</strong>g>An</str<strong>on</strong>g>d <str<strong>on</strong>g>the</str<strong>on</strong>g>re it was again:<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> c<strong>on</strong>necti<strong>on</strong> between water and <str<strong>on</strong>g>the</str<strong>on</strong>g> cosmos. The scientists<br />

c<strong>on</strong>firmed that <str<strong>on</strong>g>the</str<strong>on</strong>g> structure <str<strong>on</strong>g>of</str<strong>on</strong>g> water undergoes lasting change<br />

during Grander ® revitalisati<strong>on</strong>. They supplied pro<str<strong>on</strong>g>of</str<strong>on</strong>g> that <str<strong>on</strong>g>the</str<strong>on</strong>g>re<br />

is vertical compressi<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> hexag<strong>on</strong>al units. Thus water that<br />

has underg<strong>on</strong>e Grander ® revitalisati<strong>on</strong> has qualities that are<br />

comparable with those <str<strong>on</strong>g>of</str<strong>on</strong>g> “oscillating quartz”: <str<strong>on</strong>g>the</str<strong>on</strong>g> vertical<br />

structures act as antennas that pick up <str<strong>on</strong>g>the</str<strong>on</strong>g> special frequencies<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> cosmic energy and transfer it (like a transmitter) <str<strong>on</strong>g>to</str<strong>on</strong>g> water<br />

that has not been revitalised.<br />

So Johann Grander is right: water is c<strong>on</strong>nected with <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

cosmos.<br />

To me, it has been very interesting <str<strong>on</strong>g>to</str<strong>on</strong>g> observe <str<strong>on</strong>g>the</str<strong>on</strong>g> uncomplicated<br />

relati<strong>on</strong>ship that so-called “natural researchers” such as<br />

Vik<str<strong>on</strong>g>to</str<strong>on</strong>g>r Schauberger and Johann Grander have <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> phenomen<strong>on</strong><br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> water. They succeed in discovering relati<strong>on</strong>ships that are<br />

initially incomprehensible <str<strong>on</strong>g>to</str<strong>on</strong>g> “qualified” scientists. This is also<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> reas<strong>on</strong> why I want <str<strong>on</strong>g>to</str<strong>on</strong>g> dedicate <str<strong>on</strong>g>the</str<strong>on</strong>g> present work <str<strong>on</strong>g>to</str<strong>on</strong>g> Johann<br />

Grander. With his highly descriptive language he has helped me<br />

find an entirely new relati<strong>on</strong>ship <str<strong>on</strong>g>to</str<strong>on</strong>g> natural phenomena. Thus<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> investigati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> water as a “cosmic thing” is a matter that<br />

will c<strong>on</strong>tinue <str<strong>on</strong>g>to</str<strong>on</strong>g> fascinate me for a l<strong>on</strong>g time <str<strong>on</strong>g>to</str<strong>on</strong>g> come.<br />

Dr. Horst Felsch,<br />

Academically Qualified Engineer<br />

April 2000<br />

In order <str<strong>on</strong>g>to</str<strong>on</strong>g> invalidate this noti<strong>on</strong> that “faith” is required<br />

and also <str<strong>on</strong>g>to</str<strong>on</strong>g> give <str<strong>on</strong>g>the</str<strong>on</strong>g> users trust and c<strong>on</strong>fidence in Grander ®<br />

<strong>Technology</strong>, <str<strong>on</strong>g>the</str<strong>on</strong>g> results presented in this brochure have been<br />

obtained using scientifically recognised test methods. Thus <str<strong>on</strong>g>the</str<strong>on</strong>g>y<br />

can be checked by any properly equipped labora<str<strong>on</strong>g>to</str<strong>on</strong>g>ry .<br />

In this regard, I would like <str<strong>on</strong>g>to</str<strong>on</strong>g> make <str<strong>on</strong>g>the</str<strong>on</strong>g> following important<br />

point: <str<strong>on</strong>g>the</str<strong>on</strong>g> studies described here were written primarily for <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

“interested layman” ra<str<strong>on</strong>g>the</str<strong>on</strong>g>r than for <str<strong>on</strong>g>the</str<strong>on</strong>g> scientist. Despite <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

complicated c<strong>on</strong>text it was intended <str<strong>on</strong>g>to</str<strong>on</strong>g> keep <str<strong>on</strong>g>the</str<strong>on</strong>g> text universally<br />

comprehensible. The “complete versi<strong>on</strong>” <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g>se studies will<br />

be published next year in book form.<br />

<str<strong>on</strong>g>An</str<strong>on</strong>g>d <str<strong>on</strong>g>the</str<strong>on</strong>g> cosmic thing? Here as well, we have made c<strong>on</strong>siderable<br />

progress. In 1997, a research c<strong>on</strong>tract related <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

Grander ® <strong>Technology</strong> was awarded <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> vice president <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

Russian Academy <str<strong>on</strong>g>of</str<strong>on</strong>g> Natural Sciences, Pr<str<strong>on</strong>g>of</str<strong>on</strong>g>. Yuri Rachmanin.<br />

3


Microbiological Effects <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ® <strong>Technology</strong><br />

Pin Point Formati<strong>on</strong><br />

First investigati<strong>on</strong>s have proved that <strong>Grander®</strong> <strong>Technology</strong> changes <str<strong>on</strong>g>the</str<strong>on</strong>g> cluster structure<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> water. Above all, this should effect bacteria, which have maintained a living<br />

relati<strong>on</strong>ship (biocoenosis) with water from time immemorial. Changes in <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

cluster structure should <str<strong>on</strong>g>of</str<strong>on</strong>g> necessity also lead <str<strong>on</strong>g>to</str<strong>on</strong>g> bacteriological changes.<br />

This was <str<strong>on</strong>g>the</str<strong>on</strong>g> basic premise for <str<strong>on</strong>g>the</str<strong>on</strong>g> investigati<strong>on</strong>s that are described below.<br />

Pho<str<strong>on</strong>g>to</str<strong>on</strong>g>s: Dr. Felsch<br />

The expressi<strong>on</strong> “pin points” is taken from practical<br />

life and refers <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> size <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> bacteria. It is applied<br />

<str<strong>on</strong>g>to</str<strong>on</strong>g> bacterial col<strong>on</strong>ies that are particularly small,<br />

and <str<strong>on</strong>g>the</str<strong>on</strong>g> size <str<strong>on</strong>g>of</str<strong>on</strong>g> a pin-head is a well-chosen comparis<strong>on</strong>.<br />

Until <strong>on</strong>ly a few years ago <str<strong>on</strong>g>the</str<strong>on</strong>g> expressi<strong>on</strong> “pin points”<br />

was relatively little known in water research. Pin points were<br />

described in a dissertati<strong>on</strong> submitted in 1972 by an Iranian<br />

student, Khodaia Mohaled. The title <str<strong>on</strong>g>of</str<strong>on</strong>g> his dissertati<strong>on</strong> was<br />

Oligocarbophile: Micro-organisms in Plus Lake. The literal translati<strong>on</strong><br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> name oligocarbophile bacteria implies that <str<strong>on</strong>g>the</str<strong>on</strong>g>y<br />

like <strong>on</strong>ly a small amount <str<strong>on</strong>g>of</str<strong>on</strong>g> carb<strong>on</strong>; thus <str<strong>on</strong>g>the</str<strong>on</strong>g>y are typical<br />

water bacteria, which have adapted <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> very low levels<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> nutrients found in water. In flowing waters (i.e. streams)<br />

it is quite usual <str<strong>on</strong>g>to</str<strong>on</strong>g> find pin points within <str<strong>on</strong>g>the</str<strong>on</strong>g> spectrum <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> living col<strong>on</strong>ies <str<strong>on</strong>g>of</str<strong>on</strong>g> bacteria. This is seldom <str<strong>on</strong>g>the</str<strong>on</strong>g> case in<br />

drinking water.<br />

In <str<strong>on</strong>g>the</str<strong>on</strong>g> meantime, <str<strong>on</strong>g>the</str<strong>on</strong>g> expressi<strong>on</strong> “pin points” has been<br />

admitted <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> textbooks <strong>on</strong> microbiology, such as Hans<br />

G. Schlegel’s General Microbiology, published by Georg Thieme<br />

A page from <str<strong>on</strong>g>the</str<strong>on</strong>g> textbook General Microbiology, in which <str<strong>on</strong>g>the</str<strong>on</strong>g> expressi<strong>on</strong><br />

pin points is described. In <str<strong>on</strong>g>the</str<strong>on</strong>g> illustrati<strong>on</strong> <strong>on</strong>e can see large white col<strong>on</strong>ies<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> “wild” type – which is <str<strong>on</strong>g>the</str<strong>on</strong>g> original bacterium. The small white dots<br />

are pin points. The difference in size is quite apparent.<br />

Verlag in Stuttgart, Germany. It c<strong>on</strong>tains an illustrati<strong>on</strong><br />

that shows <str<strong>on</strong>g>the</str<strong>on</strong>g> difference in size between “wild” col<strong>on</strong>ies<br />

and pin point col<strong>on</strong>ies. In <str<strong>on</strong>g>the</str<strong>on</strong>g> present report, I would like <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

use bacteriological methods <str<strong>on</strong>g>to</str<strong>on</strong>g> dem<strong>on</strong>strate that <str<strong>on</strong>g>the</str<strong>on</strong>g> massive<br />

appearance <str<strong>on</strong>g>of</str<strong>on</strong>g> pin points provides clear evidence <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ®<br />

revitalisati<strong>on</strong>.<br />

Pho<str<strong>on</strong>g>to</str<strong>on</strong>g> with <str<strong>on</strong>g>the</str<strong>on</strong>g> kind permissi<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> publisher<br />

4


The Ability <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>Water</str<strong>on</strong>g><br />

<str<strong>on</strong>g>to</str<strong>on</strong>g> Purify Itself<br />

Polluted water has usually lost many <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> qualities<br />

that unpolluted water still possesses, e.g. <str<strong>on</strong>g>the</str<strong>on</strong>g> ability <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

assimilate sufficient quantities <str<strong>on</strong>g>of</str<strong>on</strong>g> oxygen. O<str<strong>on</strong>g>the</str<strong>on</strong>g>r qualities<br />

include freshness, taste and smell. Polluted water is <str<strong>on</strong>g>of</str<strong>on</strong>g>ten no<br />

l<strong>on</strong>ger capable <str<strong>on</strong>g>of</str<strong>on</strong>g> purifying itself. The bacteria in polluted<br />

waters are unable <str<strong>on</strong>g>to</str<strong>on</strong>g> break down <str<strong>on</strong>g>the</str<strong>on</strong>g> large quantity <str<strong>on</strong>g>of</str<strong>on</strong>g> nutrients<br />

found in <str<strong>on</strong>g>the</str<strong>on</strong>g>se waters. Usually <str<strong>on</strong>g>the</str<strong>on</strong>g> necessary oxygen<br />

is lacking, so that anaerobic processes are more likely <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

take place, creating unpleasant smells. Thus our wastewater<br />

treatment plants need huge quantities <str<strong>on</strong>g>of</str<strong>on</strong>g> oxygen from <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

air in order <str<strong>on</strong>g>to</str<strong>on</strong>g> stimulate micro-organisms in <str<strong>on</strong>g>the</str<strong>on</strong>g> “revitalisati<strong>on</strong><br />

pool” <str<strong>on</strong>g>to</str<strong>on</strong>g> reduce <str<strong>on</strong>g>the</str<strong>on</strong>g> levels <str<strong>on</strong>g>of</str<strong>on</strong>g> polluti<strong>on</strong>.<br />

How Is <str<strong>on</strong>g>the</str<strong>on</strong>g> Bacterial Count<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>Water</str<strong>on</strong>g> Determined?<br />

For <str<strong>on</strong>g>the</str<strong>on</strong>g> determinati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> bacterial count, a method<br />

is used in which <str<strong>on</strong>g>the</str<strong>on</strong>g> bacteria are cultured <strong>on</strong> an artificial<br />

culture medium. Individual bacteria create larger col<strong>on</strong>ies<br />

that can be seen with <str<strong>on</strong>g>the</str<strong>on</strong>g> naked eye and can thus be counted<br />

without <str<strong>on</strong>g>the</str<strong>on</strong>g> use <str<strong>on</strong>g>of</str<strong>on</strong>g> a microscope. The method for determining<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> bacterial count in drinking water has been standardised<br />

al<strong>on</strong>g with <str<strong>on</strong>g>the</str<strong>on</strong>g> compositi<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> culture media <str<strong>on</strong>g>to</str<strong>on</strong>g> be<br />

used. In principal <str<strong>on</strong>g>the</str<strong>on</strong>g>re are two methods for determining<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> bacterial count: <str<strong>on</strong>g>the</str<strong>on</strong>g> plate culture method and <str<strong>on</strong>g>the</str<strong>on</strong>g> membrane<br />

filter method. All <str<strong>on</strong>g>the</str<strong>on</strong>g> investigati<strong>on</strong>s carried out by me<br />

were c<strong>on</strong>ducted using <str<strong>on</strong>g>the</str<strong>on</strong>g> membrane filter method. Thus I<br />

would like <str<strong>on</strong>g>to</str<strong>on</strong>g> explain <str<strong>on</strong>g>the</str<strong>on</strong>g> method in greater detail.<br />

In determining <str<strong>on</strong>g>the</str<strong>on</strong>g> bacterial count, 1 ml <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> drinking<br />

water being tested (or 1 ml <str<strong>on</strong>g>of</str<strong>on</strong>g> an appropriate diluti<strong>on</strong>) is<br />

Robert Koch in <str<strong>on</strong>g>the</str<strong>on</strong>g> year 1886. He was <str<strong>on</strong>g>the</str<strong>on</strong>g> first <str<strong>on</strong>g>to</str<strong>on</strong>g> succeed in growing<br />

bacteria <strong>on</strong> an “artificial” culture medium. He used meat soup as a medium<br />

in <str<strong>on</strong>g>the</str<strong>on</strong>g> petri dishes that can be seen in this pho<str<strong>on</strong>g>to</str<strong>on</strong>g>graph. This allowed him<br />

<str<strong>on</strong>g>to</str<strong>on</strong>g> isolate and culture disease-causing agents (pathogens).<br />

drawn through a membrane filter under sterile c<strong>on</strong>diti<strong>on</strong>s.<br />

The bacteria and fungi c<strong>on</strong>tained in <str<strong>on</strong>g>the</str<strong>on</strong>g> water are trapped<br />

by <str<strong>on</strong>g>the</str<strong>on</strong>g> filter, which is <str<strong>on</strong>g>the</str<strong>on</strong>g>n laid <strong>on</strong><str<strong>on</strong>g>to</str<strong>on</strong>g> an artificial culture medium.<br />

The filter draws nutrients from <str<strong>on</strong>g>the</str<strong>on</strong>g> culture medium so<br />

that <str<strong>on</strong>g>the</str<strong>on</strong>g> bacteria and fungi found <strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> surface <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> filter<br />

easily grow and multiply. The bacteria multiply in a c<strong>on</strong>centric<br />

pattern, ideally spreading from a single bacterium and<br />

forming col<strong>on</strong>ies that are visible <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> naked eye. The reas<strong>on</strong><br />

this happens is that some 100,000 bacteria can be generated<br />

from a single bacterium in a period <str<strong>on</strong>g>of</str<strong>on</strong>g> <strong>on</strong>ly 48 hours (depending<br />

<strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> reproductive period). The formati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> visible<br />

col<strong>on</strong>ies makes it possible <str<strong>on</strong>g>to</str<strong>on</strong>g> take a bacteria count even without<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> assistance <str<strong>on</strong>g>of</str<strong>on</strong>g> a microscope. The great advantage <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

this method is that <strong>on</strong>ly those bacteria and fungi capable <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

reproducing are counted, i.e. <str<strong>on</strong>g>the</str<strong>on</strong>g> <strong>on</strong>ly germs that represent<br />

a possible danger in drinking water.<br />

Pho<str<strong>on</strong>g>to</str<strong>on</strong>g>: Institut Pasteur, Paris<br />

What Causes Pin Points To Form?<br />

Pin points are created in o<str<strong>on</strong>g>the</str<strong>on</strong>g>r<br />

ways besides <str<strong>on</strong>g>the</str<strong>on</strong>g> use <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ®<br />

<strong>Technology</strong>. Pin points can also<br />

form when a water sample is subjected<br />

<str<strong>on</strong>g>to</str<strong>on</strong>g> ultrasound over a l<strong>on</strong>ger<br />

period <str<strong>on</strong>g>of</str<strong>on</strong>g> time. Under <str<strong>on</strong>g>the</str<strong>on</strong>g> influence<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> very high frequency sound waves,<br />

bacteria “families” are fragmented<br />

and scattered. The individual col<strong>on</strong>ies<br />

thus formed <str<strong>on</strong>g>of</str<strong>on</strong>g>ten take <str<strong>on</strong>g>the</str<strong>on</strong>g> form <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

extremely tiny col<strong>on</strong>ies, thus becoming<br />

pin points.<br />

A sec<strong>on</strong>d possibility <str<strong>on</strong>g>of</str<strong>on</strong>g> producing<br />

pin points is <str<strong>on</strong>g>the</str<strong>on</strong>g> use <str<strong>on</strong>g>of</str<strong>on</strong>g> sub - lethal<br />

radiati<strong>on</strong> with ultraviolet light. Also<br />

in this case, <str<strong>on</strong>g>the</str<strong>on</strong>g> introducti<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> energy<br />

can tear apart <str<strong>on</strong>g>the</str<strong>on</strong>g> aggregati<strong>on</strong>s<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> bacteria.<br />

Grander ® <strong>Technology</strong> uses a completely<br />

different principle in inducing<br />

pin point formati<strong>on</strong>. It does not<br />

depend <strong>on</strong> massive doses <str<strong>on</strong>g>of</str<strong>on</strong>g> energy.<br />

Ra<str<strong>on</strong>g>the</str<strong>on</strong>g>r, according <str<strong>on</strong>g>to</str<strong>on</strong>g> our present state<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> knowledge, by altering <str<strong>on</strong>g>the</str<strong>on</strong>g> cluster<br />

structure <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> water <str<strong>on</strong>g>the</str<strong>on</strong>g> behaviour<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> micro-organisms is fundamentally<br />

changed. Thus <str<strong>on</strong>g>the</str<strong>on</strong>g> Grander ® pin<br />

points that form are a reacti<strong>on</strong> <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

structural change <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> water and not<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> result <str<strong>on</strong>g>of</str<strong>on</strong>g> some process that leads<br />

<str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> destructi<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> bacterial<br />

aggregates.<br />

For this reas<strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> pin points that<br />

result from ultrasound influence are<br />

fundamentally different from Grander<br />

® pin points. If a water sample is<br />

subjected <str<strong>on</strong>g>to</str<strong>on</strong>g> ultrasound and <str<strong>on</strong>g>the</str<strong>on</strong>g>n<br />

tested for bacteria, tiny col<strong>on</strong>ies can<br />

be found almost immediately, just<br />

as so<strong>on</strong> as <str<strong>on</strong>g>the</str<strong>on</strong>g>y have had time <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

multiply.<br />

Following c<strong>on</strong>tact <str<strong>on</strong>g>of</str<strong>on</strong>g> a water<br />

sample with Grander ® <strong>Technology</strong> it<br />

takes at least two <str<strong>on</strong>g>to</str<strong>on</strong>g> three days before<br />

pin points are formed.<br />

5


Distinguishing Features between Tiny Col<strong>on</strong>ies, Created by <str<strong>on</strong>g>the</str<strong>on</strong>g> Effects<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> UV Radiati<strong>on</strong> or Ultrasound, and Grander ® Pin Points:<br />

Pin point formati<strong>on</strong> due <str<strong>on</strong>g>to</str<strong>on</strong>g> Pin point formati<strong>on</strong> due <str<strong>on</strong>g>to</str<strong>on</strong>g> Grander ®<br />

ultrasound or UV radiati<strong>on</strong><br />

Pin point formati<strong>on</strong> induced by mechanical sound energy or heavy altered microbiological behaviour; no<br />

radiati<strong>on</strong>, causing splitting <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

splitting <str<strong>on</strong>g>of</str<strong>on</strong>g> bacteria aggregati<strong>on</strong>s in<str<strong>on</strong>g>to</str<strong>on</strong>g><br />

aggregati<strong>on</strong>s in<str<strong>on</strong>g>to</str<strong>on</strong>g> individual bacteria individual parts, but alterati<strong>on</strong> in<str<strong>on</strong>g>to</str<strong>on</strong>g><br />

harmless but very active new forms<br />

Occurrence immediate not until 2 <str<strong>on</strong>g>to</str<strong>on</strong>g> 3 days after first<br />

c<strong>on</strong>tact with Grander ® <strong>Technology</strong><br />

Substrate utilisati<strong>on</strong> same as “wild” bacteria different utilisati<strong>on</strong> than<br />

“wild” bacteria<br />

Breakdown <str<strong>on</strong>g>of</str<strong>on</strong>g> carb<strong>on</strong> c<strong>on</strong>tent no increased breakdown and/or increased breakdown and lowering<br />

and lowering <str<strong>on</strong>g>of</str<strong>on</strong>g> AOC c<strong>on</strong>tent no lowering <str<strong>on</strong>g>of</str<strong>on</strong>g> AOC c<strong>on</strong>tent <str<strong>on</strong>g>of</str<strong>on</strong>g> AOC c<strong>on</strong>tent, resulting in<br />

(food c<strong>on</strong>tent)<br />

purificati<strong>on</strong> and improved water<br />

The Pin Point Formati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> Bacteria,<br />

Using Grander ® <strong>Technology</strong><br />

The pin point formati<strong>on</strong> that gives revitalised water completely<br />

new qualities is <strong>on</strong>e <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> most fantastic phenomena<br />

related <str<strong>on</strong>g>to</str<strong>on</strong>g> Grander ® <strong>Technology</strong>. With amazing speed<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g>se pin points break down <str<strong>on</strong>g>the</str<strong>on</strong>g> organic c<strong>on</strong>tent dissolved<br />

in <str<strong>on</strong>g>the</str<strong>on</strong>g> water, thus purifying it. In this manner <str<strong>on</strong>g>the</str<strong>on</strong>g> water<br />

loses its perishability and regains all those qualities that it<br />

had in an unpolluted state.<br />

The as<str<strong>on</strong>g>to</str<strong>on</strong>g>nishing thing about this phenomen<strong>on</strong> is that<br />

water that has <strong>on</strong>ce been revitalised by <str<strong>on</strong>g>the</str<strong>on</strong>g> Grander ® method<br />

always retains <str<strong>on</strong>g>the</str<strong>on</strong>g>se qualities. If a bottle <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ®<br />

water is polluted, for example, by adding a drop <str<strong>on</strong>g>of</str<strong>on</strong>g> a sterile,<br />

artificial culture medium, <str<strong>on</strong>g>the</str<strong>on</strong>g> number <str<strong>on</strong>g>of</str<strong>on</strong>g> pin points increases<br />

within <strong>on</strong>ly a few hours. They quickly break down <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

source <str<strong>on</strong>g>of</str<strong>on</strong>g> carb<strong>on</strong>, thus purifying <str<strong>on</strong>g>the</str<strong>on</strong>g> water <strong>on</strong>ce again.<br />

With regard <str<strong>on</strong>g>to</str<strong>on</strong>g> pin point formati<strong>on</strong> Grander ® <strong>Technology</strong><br />

is highly specific. I have tested many <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> water systems<br />

available <strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> market. They promise water activati<strong>on</strong> or<br />

similar effects, but not a single <strong>on</strong>e <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g>se technologies was<br />

able <str<strong>on</strong>g>to</str<strong>on</strong>g> promote pin point formati<strong>on</strong> in Johann Grander’s<br />

sense.<br />

Because I c<strong>on</strong>tinue <str<strong>on</strong>g>to</str<strong>on</strong>g> refer <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> high number <str<strong>on</strong>g>of</str<strong>on</strong>g> pin<br />

points, I should point out <strong>on</strong>ce again <str<strong>on</strong>g>the</str<strong>on</strong>g> harmlessness<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g>se pin points. The changes <str<strong>on</strong>g>the</str<strong>on</strong>g>y undergo makes<br />

pin points lose <str<strong>on</strong>g>the</str<strong>on</strong>g> qualities <str<strong>on</strong>g>of</str<strong>on</strong>g> resistance that <str<strong>on</strong>g>the</str<strong>on</strong>g> “wild”<br />

bacteria <strong>on</strong>ce had. It is hardly possible for pin points <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

“Determinati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

Microbes Capable <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

Reproducti<strong>on</strong>, Using <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

Membrane Filter Method”<br />

This procedure is described in DIN<br />

38411-K5. The membrane filters c<strong>on</strong>sist<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> chemically modified cellulose and<br />

have a pore size <str<strong>on</strong>g>of</str<strong>on</strong>g> 0.45 µm. These pores<br />

are thus <str<strong>on</strong>g>to</str<strong>on</strong>g>o small for most bacteria <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

pass through so that <str<strong>on</strong>g>the</str<strong>on</strong>g>y are trapped<br />

<strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> surface <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> filter. In each test,<br />

1 ml <str<strong>on</strong>g>of</str<strong>on</strong>g> sample liquid is drawn through<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> membrane filter, which is <str<strong>on</strong>g>the</str<strong>on</strong>g>n laid<br />

<strong>on</strong><str<strong>on</strong>g>to</str<strong>on</strong>g> a standard culture medium.<br />

Membrane filter (diameter 50 mm, thickness<br />

.01 mm) with a printed grid for counting. Because<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g>ir size, bacteria and fungi cannot<br />

pass through <str<strong>on</strong>g>the</str<strong>on</strong>g> pores and are trapped by <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

filter. After 24, 48 and 96 hours <str<strong>on</strong>g>the</str<strong>on</strong>g> col<strong>on</strong>ies<br />

that have formed are counted.<br />

After <str<strong>on</strong>g>the</str<strong>on</strong>g> sample has been drawn through <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

membrane filter, it is “rolled” <strong>on</strong><str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> surface<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> a sterile culture medium. During <str<strong>on</strong>g>the</str<strong>on</strong>g> incubati<strong>on</strong><br />

period <str<strong>on</strong>g>the</str<strong>on</strong>g> bacteria found <strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> filter are<br />

nourished by <str<strong>on</strong>g>the</str<strong>on</strong>g> culture medium, enabling<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g>m <str<strong>on</strong>g>to</str<strong>on</strong>g> reproduce and form col<strong>on</strong>ies.<br />

6


Pro<str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>of</str<strong>on</strong>g> Effectiveness<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> Revitalisati<strong>on</strong><br />

The completely different images<br />

shown here make <str<strong>on</strong>g>the</str<strong>on</strong>g> remarkable<br />

nature <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ®<br />

<strong>Technology</strong> with respect <str<strong>on</strong>g>to</str<strong>on</strong>g> pin<br />

point formati<strong>on</strong> perfectly clear.<br />

This discovery has made it possible<br />

<str<strong>on</strong>g>to</str<strong>on</strong>g> develop a comprehensible<br />

scientific procedure for<br />

determining <str<strong>on</strong>g>the</str<strong>on</strong>g> effectiveness<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ® <strong>Technology</strong> in<br />

water systems.<br />

Example <str<strong>on</strong>g>of</str<strong>on</strong>g> unrevitalised water with acceptable<br />

bacteria levels. In order <str<strong>on</strong>g>to</str<strong>on</strong>g> create more col<strong>on</strong>ies,<br />

50 ml was drawn through <str<strong>on</strong>g>the</str<strong>on</strong>g> filter, which was<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g>n incubated <strong>on</strong> standard nutrient agar for<br />

72 hours at 22 degrees Celsius. The various<br />

sizes and shapes <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> col<strong>on</strong>ies are typical.<br />

Following revitalisati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> water from St Ulrich<br />

am Pillersee and two resting days, this typical<br />

image was formed after 72 hours <str<strong>on</strong>g>of</str<strong>on</strong>g> incubati<strong>on</strong><br />

at 22 degrees Celsius. Only a few “wild” col<strong>on</strong>ies<br />

remain. A large number <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ® pin points<br />

predominate.<br />

multiply at 37 degrees Celsius no matter how good <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

culture medium is. Thus we may be certain that drinking<br />

water can never have a harmful effect, no matter how many<br />

pin points it may c<strong>on</strong>tain, because both normal body<br />

temperature and s<str<strong>on</strong>g>to</str<strong>on</strong>g>mach acid would kill <str<strong>on</strong>g>the</str<strong>on</strong>g>se pin points<br />

immediately. In additi<strong>on</strong>, an increase in <str<strong>on</strong>g>the</str<strong>on</strong>g> number <str<strong>on</strong>g>of</str<strong>on</strong>g> pin<br />

points does not imply an increase in disease germs (which<br />

are not permitted by law).<br />

Details <strong>on</strong> Grander ®<br />

Pin point Formati<strong>on</strong><br />

As early as 1993 I was able <str<strong>on</strong>g>to</str<strong>on</strong>g> establish that bacteria<br />

c<strong>on</strong>tained in water form pin points as a result <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ®<br />

<strong>Technology</strong>. If a sample <str<strong>on</strong>g>of</str<strong>on</strong>g> drinking water, for example,<br />

comes in<str<strong>on</strong>g>to</str<strong>on</strong>g> c<strong>on</strong>tact with Grander ® <strong>Technology</strong>, <strong>on</strong>e<br />

can observe <str<strong>on</strong>g>the</str<strong>on</strong>g> pin point formati<strong>on</strong> taking place within<br />

about two <str<strong>on</strong>g>to</str<strong>on</strong>g> three days (that’s how l<strong>on</strong>g <str<strong>on</strong>g>the</str<strong>on</strong>g> bacteria<br />

take <str<strong>on</strong>g>to</str<strong>on</strong>g> change). The bacterial picture becomes uniform:<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g>re are no l<strong>on</strong>ger any large “wild” col<strong>on</strong>ies,<br />

but <strong>on</strong>ly small <strong>on</strong>es <str<strong>on</strong>g>of</str<strong>on</strong>g> pin-head size.<br />

This pin point formati<strong>on</strong>, which is typical <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander<br />

® <strong>Technology</strong>, has meanwhile also become <str<strong>on</strong>g>the</str<strong>on</strong>g> most<br />

important method for dem<strong>on</strong>strating <str<strong>on</strong>g>the</str<strong>on</strong>g> effectiveness<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ® <strong>Technology</strong>.<br />

Since 1993 I have c<strong>on</strong>ducted tests <strong>on</strong> some 2,000 water<br />

samples and found c<strong>on</strong>firmati<strong>on</strong> time and time again<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> pin point formati<strong>on</strong> caused by Grander ® <strong>Technology</strong>.<br />

This pin point formati<strong>on</strong> has also been c<strong>on</strong>firmed<br />

After an incubati<strong>on</strong> period <str<strong>on</strong>g>of</str<strong>on</strong>g> 96 hours, col<strong>on</strong>ies<br />

are formed that can be seen with <str<strong>on</strong>g>the</str<strong>on</strong>g> naked eye<br />

and thus can be counted. This drinking water<br />

had 30 CFU/ml (CFU = col<strong>on</strong>y forming units).<br />

The guideline that may not be exceeded under<br />

Austrian regulati<strong>on</strong>s sets a limit <str<strong>on</strong>g>of</str<strong>on</strong>g> 100 CFU/ml.<br />

<str<strong>on</strong>g>Water</str<strong>on</strong>g> revitalised using Grander ® <strong>Technology</strong><br />

shows typical pin point formati<strong>on</strong> (<str<strong>on</strong>g>the</str<strong>on</strong>g>se are<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> very small white col<strong>on</strong>ies). The unrevitalised<br />

col<strong>on</strong>ies = “wild” or mo<str<strong>on</strong>g>the</str<strong>on</strong>g>r col<strong>on</strong>ies are substantially<br />

larger and have been coloured red by<br />

a dye in <str<strong>on</strong>g>the</str<strong>on</strong>g> culture medium.<br />

When <str<strong>on</strong>g>the</str<strong>on</strong>g> water has been completely revitalised<br />

by Grander ® <strong>Technology</strong>, <strong>on</strong>ly pin points<br />

are found <strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> membrane filter. No “wild”<br />

col<strong>on</strong>ies are <str<strong>on</strong>g>to</str<strong>on</strong>g> be seen.<br />

7


Alterati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> Col<strong>on</strong>y Image<br />

by <strong>Grander®</strong> Revitalisati<strong>on</strong><br />

First, unrevitalised drinking water was incubated for 96<br />

hours at 22 degrees Celsius. After revitalisati<strong>on</strong>, <str<strong>on</strong>g>the</str<strong>on</strong>g> col<strong>on</strong>y<br />

image clearly changed, as may be seen in <str<strong>on</strong>g>the</str<strong>on</strong>g> pho<str<strong>on</strong>g>to</str<strong>on</strong>g>graphs<br />

taken after 5, 10, 15 days and 4 weeks.<br />

5 ml <str<strong>on</strong>g>of</str<strong>on</strong>g> unrevitalised drinking water<br />

was drawn through <str<strong>on</strong>g>the</str<strong>on</strong>g> membrane<br />

filter and incubated for 96 hours at<br />

22 degrees Celsius. One can see <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

diverse nature <str<strong>on</strong>g>of</str<strong>on</strong>g> col<strong>on</strong>y formati<strong>on</strong>, <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

varying colour (because <str<strong>on</strong>g>of</str<strong>on</strong>g> a colorant<br />

added <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> culture medium) and <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

varying size <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> col<strong>on</strong>ies. This water<br />

afterwards was revitalised with <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

help <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ® <strong>Technology</strong>.<br />

Five days after <str<strong>on</strong>g>the</str<strong>on</strong>g> start <str<strong>on</strong>g>of</str<strong>on</strong>g> revitalisati<strong>on</strong>,<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> bacteriological picture has<br />

already changed. The size <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> col<strong>on</strong>y<br />

formati<strong>on</strong>s no l<strong>on</strong>ger varies, and<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> colours have become uniform.<br />

Ten days after <str<strong>on</strong>g>the</str<strong>on</strong>g> start <str<strong>on</strong>g>of</str<strong>on</strong>g> revitalisati<strong>on</strong>,<br />

countless pin points have formed.<br />

The col<strong>on</strong>ies are so close <str<strong>on</strong>g>to</str<strong>on</strong>g>ge<str<strong>on</strong>g>the</str<strong>on</strong>g>r<br />

that <strong>on</strong>ly <str<strong>on</strong>g>the</str<strong>on</strong>g> outer edge <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> thick<br />

growth is capable <str<strong>on</strong>g>of</str<strong>on</strong>g> accepting dye.<br />

There is very thick growth at <str<strong>on</strong>g>the</str<strong>on</strong>g> interior<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> filter area.<br />

Fifteen days after <str<strong>on</strong>g>the</str<strong>on</strong>g> start <str<strong>on</strong>g>of</str<strong>on</strong>g> revitalisati<strong>on</strong><br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> number <str<strong>on</strong>g>of</str<strong>on</strong>g> pin points becomes<br />

smaller. This is a sign that <str<strong>on</strong>g>the</str<strong>on</strong>g> food supply<br />

has almost been exhausted and <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

bacteriological purificati<strong>on</strong> has for <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

most part been completed.<br />

by o<str<strong>on</strong>g>the</str<strong>on</strong>g>r university institutes and by Pr<str<strong>on</strong>g>of</str<strong>on</strong>g>. Dr. Rachmanin,<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> man resp<strong>on</strong>sible for <str<strong>on</strong>g>the</str<strong>on</strong>g> purity <str<strong>on</strong>g>of</str<strong>on</strong>g> Moscow’s city water<br />

supply.<br />

What Qualities Are To Be Found<br />

in <str<strong>on</strong>g>the</str<strong>on</strong>g> Pin Points Resulting from<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> Use <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ® <strong>Technology</strong>?<br />

It is truly fascinating how <str<strong>on</strong>g>the</str<strong>on</strong>g> number <str<strong>on</strong>g>of</str<strong>on</strong>g> pin points<br />

increases sharply within just a few days <str<strong>on</strong>g>of</str<strong>on</strong>g> revitalisati<strong>on</strong> with<br />

Grander ® <strong>Technology</strong>. One might easily assume that <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

pin points I describe are <str<strong>on</strong>g>the</str<strong>on</strong>g> result <str<strong>on</strong>g>of</str<strong>on</strong>g> some externally caused<br />

infecti<strong>on</strong> or c<strong>on</strong>taminati<strong>on</strong>. But this assumpti<strong>on</strong> can be<br />

refuted in a very simple experiment:<br />

A sealed bottle, made <str<strong>on</strong>g>of</str<strong>on</strong>g> glass or plastic and c<strong>on</strong>taining<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> water <str<strong>on</strong>g>to</str<strong>on</strong>g> be tested, is placed for two <str<strong>on</strong>g>to</str<strong>on</strong>g> three days next<br />

<str<strong>on</strong>g>to</str<strong>on</strong>g> a bottle <str<strong>on</strong>g>of</str<strong>on</strong>g> Original Grander ® <str<strong>on</strong>g>Water</str<strong>on</strong>g>. Bacteriological<br />

examinati<strong>on</strong> after an appropriate period <str<strong>on</strong>g>of</str<strong>on</strong>g> incubati<strong>on</strong><br />

reveals <str<strong>on</strong>g>the</str<strong>on</strong>g> pin point formati<strong>on</strong> described here. A blind test<br />

with a sec<strong>on</strong>d bottle filled under <str<strong>on</strong>g>the</str<strong>on</strong>g> same c<strong>on</strong>diti<strong>on</strong>s but<br />

with no c<strong>on</strong>tact with Grander ® <strong>Technology</strong> dem<strong>on</strong>strates<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> difference: <str<strong>on</strong>g>the</str<strong>on</strong>g>re is no formati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> pin points. The<br />

<strong>on</strong>ly case in which pin points are not formed is if <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

water has been str<strong>on</strong>gly chlorinated and thus was bacteriologically<br />

dead when <str<strong>on</strong>g>the</str<strong>on</strong>g> sample was taken. In <str<strong>on</strong>g>the</str<strong>on</strong>g> membrane<br />

filter method, if <str<strong>on</strong>g>the</str<strong>on</strong>g> pin points form very slowly<br />

<strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> agar plate, i.e. <strong>on</strong>ly after 96 hours <str<strong>on</strong>g>of</str<strong>on</strong>g> incubati<strong>on</strong>,<br />

this is always an indicati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> presence <str<strong>on</strong>g>of</str<strong>on</strong>g> inhibiting<br />

chemicals in <str<strong>on</strong>g>the</str<strong>on</strong>g> water, such as disinfectants or unusually<br />

high c<strong>on</strong>centrati<strong>on</strong>s <str<strong>on</strong>g>of</str<strong>on</strong>g> heavy metals.<br />

Thus <str<strong>on</strong>g>the</str<strong>on</strong>g> formati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> pin points using Grander ® <strong>Technology</strong><br />

cannot be explained as a bacterial infecti<strong>on</strong>. Ra<str<strong>on</strong>g>the</str<strong>on</strong>g>r<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g>re is alterati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> micro - organisms, giving <str<strong>on</strong>g>the</str<strong>on</strong>g>m<br />

completely new qualities with respect <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g>ir heightened<br />

ability <str<strong>on</strong>g>to</str<strong>on</strong>g> break down <str<strong>on</strong>g>the</str<strong>on</strong>g> bacterial nutrients c<strong>on</strong>tained in<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> water.<br />

Alterati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> Qualities<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> Resistance <str<strong>on</strong>g>of</str<strong>on</strong>g> Pin Points<br />

Four weeks after <str<strong>on</strong>g>the</str<strong>on</strong>g> start <str<strong>on</strong>g>of</str<strong>on</strong>g> revitalisati<strong>on</strong><br />

using Grander ® <strong>Technology</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

food supplies have been completely<br />

exhausted. The pin points have died.<br />

The water is biologically pure. Because<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> sharply lower levels <str<strong>on</strong>g>of</str<strong>on</strong>g> assimilable<br />

organic carb<strong>on</strong> (AOC), bacteria can<br />

no l<strong>on</strong>ger reproduce (below 10 µg org.<br />

Celsius/ml).<br />

Evidently, <str<strong>on</strong>g>the</str<strong>on</strong>g> pin points resulting from Grander ® <strong>Technology</strong><br />

also lose <str<strong>on</strong>g>the</str<strong>on</strong>g> qualities <str<strong>on</strong>g>of</str<strong>on</strong>g> resistance that characterised<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> original “wild” col<strong>on</strong>y. I was able <str<strong>on</strong>g>to</str<strong>on</strong>g> establish this myself<br />

in <str<strong>on</strong>g>the</str<strong>on</strong>g> case <str<strong>on</strong>g>of</str<strong>on</strong>g> an 80,000 - litre closed heating system in<br />

a hospital. The system operated at a primary temperature<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> 55 degrees Celsius and a return - flow temperature <str<strong>on</strong>g>of</str<strong>on</strong>g> 48<br />

degrees Celsius. These are temperatures at which aquatic<br />

pseudom<strong>on</strong>ads cannot survive. Never<str<strong>on</strong>g>the</str<strong>on</strong>g>less, a very high<br />

level <str<strong>on</strong>g>of</str<strong>on</strong>g> microbe c<strong>on</strong>taminati<strong>on</strong> was found in <str<strong>on</strong>g>the</str<strong>on</strong>g> circulating<br />

water. Apparently, high selecti<strong>on</strong> pressures had result-<br />

8


ed in <str<strong>on</strong>g>the</str<strong>on</strong>g> development <str<strong>on</strong>g>of</str<strong>on</strong>g> temperature-resistant plasmids<br />

(small, circular DNA-molecules), permitting survival at<br />

temperatures <str<strong>on</strong>g>of</str<strong>on</strong>g> around 50 degrees Celsius. The system was<br />

revitalised using Grander ® <strong>Technology</strong>. Six weeks later no<br />

bacteria were <str<strong>on</strong>g>to</str<strong>on</strong>g> be found in this closed heating system. (A<br />

sec<strong>on</strong>d bacteriological institute, which was asked <str<strong>on</strong>g>to</str<strong>on</strong>g> become<br />

involved in <str<strong>on</strong>g>the</str<strong>on</strong>g> experiment as a result <str<strong>on</strong>g>of</str<strong>on</strong>g> insurance issues,<br />

arrived at <str<strong>on</strong>g>the</str<strong>on</strong>g> same results.) Evidently <str<strong>on</strong>g>the</str<strong>on</strong>g> temperature<br />

resistance <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> “wild” bacteria was lost when <str<strong>on</strong>g>the</str<strong>on</strong>g>y were<br />

transformed in<str<strong>on</strong>g>to</str<strong>on</strong>g> pin points. Fundamentally, pin points<br />

formed as a result <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ® <strong>Technology</strong> show a striking<br />

sensitivity <str<strong>on</strong>g>to</str<strong>on</strong>g> temperature. When water that has been revitalised<br />

using this technology is examined and <str<strong>on</strong>g>the</str<strong>on</strong>g> culture<br />

medium has been incubated at 37 <str<strong>on</strong>g>to</str<strong>on</strong>g> 40 degrees Celsius, <str<strong>on</strong>g>the</str<strong>on</strong>g>re<br />

is no col<strong>on</strong>y formati<strong>on</strong>. However, at room temperature<br />

pin points develop <strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> culture medium in <str<strong>on</strong>g>the</str<strong>on</strong>g> usual<br />

form after 24 hours.<br />

The Utilisati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> Substrates by<br />

Grander ® Pin Points<br />

Of particular interest is <str<strong>on</strong>g>the</str<strong>on</strong>g> ability <str<strong>on</strong>g>of</str<strong>on</strong>g> pin points created<br />

through <str<strong>on</strong>g>the</str<strong>on</strong>g> use <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ® <strong>Technology</strong> <str<strong>on</strong>g>to</str<strong>on</strong>g> find new sources<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> nourishment. In <strong>on</strong>e example, Grander ® <strong>Technology</strong><br />

has been used for many years <str<strong>on</strong>g>to</str<strong>on</strong>g> improve closed cooling<br />

systems that are operated ei<str<strong>on</strong>g>the</str<strong>on</strong>g>r with pure water or with<br />

hydrocarb<strong>on</strong> coolants – mineral-oil products.<br />

One user <str<strong>on</strong>g>of</str<strong>on</strong>g> such hydrocarb<strong>on</strong> coolants had <str<strong>on</strong>g>the</str<strong>on</strong>g> idea<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> revitalising a supply barrel <str<strong>on</strong>g>of</str<strong>on</strong>g> readymade hydrocarb<strong>on</strong><br />

coolant in order <str<strong>on</strong>g>to</str<strong>on</strong>g> see what <str<strong>on</strong>g>the</str<strong>on</strong>g> effect would be. Grander ®<br />

revitalisati<strong>on</strong> equipment was suspended in a sealable 200-litre<br />

steel drum c<strong>on</strong>taining 180 litres <str<strong>on</strong>g>of</str<strong>on</strong>g> coolant emulsi<strong>on</strong>, and<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> drum was closed. It was <strong>on</strong>ly opened, when necessary, <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

take samples for bacteriological examinati<strong>on</strong>. The revitalisati<strong>on</strong><br />

process followed <str<strong>on</strong>g>the</str<strong>on</strong>g> expected bacteriological course:<br />

within <strong>on</strong>ly a short time after <str<strong>on</strong>g>the</str<strong>on</strong>g> Grander ® <strong>Technology</strong><br />

revitalisati<strong>on</strong> began, <str<strong>on</strong>g>the</str<strong>on</strong>g>re were a large number <str<strong>on</strong>g>of</str<strong>on</strong>g> pin points.<br />

A peak was reached after four weeks. After six weeks <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

number began <str<strong>on</strong>g>to</str<strong>on</strong>g> fall, reaching a minimum after eight weeks.<br />

I came <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> c<strong>on</strong>clusi<strong>on</strong> that <str<strong>on</strong>g>the</str<strong>on</strong>g> easily utilised sources <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

carb<strong>on</strong> had been exhausted.<br />

After 10 weeks, however, <str<strong>on</strong>g>the</str<strong>on</strong>g> number <str<strong>on</strong>g>of</str<strong>on</strong>g> pin points<br />

began rising again. From this point <strong>on</strong>, my labora<str<strong>on</strong>g>to</str<strong>on</strong>g>ry<br />

tests provided <str<strong>on</strong>g>the</str<strong>on</strong>g> interesting informati<strong>on</strong> that <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

mineral-oil c<strong>on</strong>tent <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> emulsi<strong>on</strong>, which had earlier<br />

been .8 per cent, was steadily decreasing. Evidently, <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

pin points, as <str<strong>on</strong>g>the</str<strong>on</strong>g>ir primary source <str<strong>on</strong>g>of</str<strong>on</strong>g> nourishment was<br />

becoming exhausted, had learned <str<strong>on</strong>g>to</str<strong>on</strong>g> crack <str<strong>on</strong>g>the</str<strong>on</strong>g> more<br />

complex carb<strong>on</strong> chain structures and use <str<strong>on</strong>g>the</str<strong>on</strong>g>m as substrates.<br />

The explanati<strong>on</strong> for this is that, in c<strong>on</strong>trast <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> usual circula<str<strong>on</strong>g>to</str<strong>on</strong>g>ry cooling systems in which nutrients<br />

are c<strong>on</strong>stantly being added, in this drum <str<strong>on</strong>g>the</str<strong>on</strong>g>re was a<br />

limited food supply, and as it became exhausted, <str<strong>on</strong>g>the</str<strong>on</strong>g> pin<br />

points began using <str<strong>on</strong>g>the</str<strong>on</strong>g> oil as a food source.<br />

For many years now in my labora<str<strong>on</strong>g>to</str<strong>on</strong>g>ry I have been<br />

checking closed cooling systems equipped with Grander ®<br />

<strong>Technology</strong>. In practice, pin point formati<strong>on</strong> does not<br />

lead <str<strong>on</strong>g>to</str<strong>on</strong>g> an undesirable breakdown <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> oily comp<strong>on</strong>ents.<br />

This is because <str<strong>on</strong>g>the</str<strong>on</strong>g> pump circulati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> cooling system<br />

provides sufficient sources <str<strong>on</strong>g>of</str<strong>on</strong>g> food that can easily be<br />

broken down.<br />

The Nutrient C<strong>on</strong>tent<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>Water</str<strong>on</strong>g> Is Lowered by<br />

Grander ® Pin Points<br />

If bacteria is <str<strong>on</strong>g>to</str<strong>on</strong>g> remain alive in drinking water, <str<strong>on</strong>g>the</str<strong>on</strong>g> water<br />

must also c<strong>on</strong>tain nutrients. In <str<strong>on</strong>g>the</str<strong>on</strong>g> 1990s, Dutch scientists<br />

set out <str<strong>on</strong>g>to</str<strong>on</strong>g> determine <str<strong>on</strong>g>the</str<strong>on</strong>g> necessary nutrient supply in water<br />

(primarily in drinking water). This nutrient package is called<br />

assimilable organic carb<strong>on</strong> (AOC) and is expressed in µg<br />

carb<strong>on</strong> equivalent per litre.<br />

The lower limit required for bacterial growth has been<br />

determined <str<strong>on</strong>g>to</str<strong>on</strong>g> be an AOC <str<strong>on</strong>g>of</str<strong>on</strong>g> 10 µg per litre. What does this<br />

mean in practice? If drinking water c<strong>on</strong>tains less than 10<br />

µg AOC per litre, <str<strong>on</strong>g>the</str<strong>on</strong>g>re are not enough nutrients. Over <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

course <str<strong>on</strong>g>of</str<strong>on</strong>g> time <str<strong>on</strong>g>the</str<strong>on</strong>g> bacteria living in <str<strong>on</strong>g>the</str<strong>on</strong>g> water will simply<br />

starve. In practice it has been determined that drinking<br />

water with an AOC c<strong>on</strong>tent <str<strong>on</strong>g>of</str<strong>on</strong>g> 10 <str<strong>on</strong>g>to</str<strong>on</strong>g> around 50 µg/l may be<br />

classified as good, having <strong>on</strong>ly a small tendency <str<strong>on</strong>g>to</str<strong>on</strong>g> support<br />

bacterial growth.<br />

At <str<strong>on</strong>g>the</str<strong>on</strong>g> Department <str<strong>on</strong>g>of</str<strong>on</strong>g> Hydraulic Engineering, Industrial<br />

<str<strong>on</strong>g>Water</str<strong>on</strong>g> Management and Preventi<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>Water</str<strong>on</strong>g> Polluti<strong>on</strong>,<br />

Agricultural University, Vienna, a bacteriological<br />

examinati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> Original Grander ® <str<strong>on</strong>g>Water</str<strong>on</strong>g> was recently<br />

c<strong>on</strong>ducted under <str<strong>on</strong>g>the</str<strong>on</strong>g> supervisi<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> Dr. Franziska Zibuschka.<br />

A water sample was bottled <strong>on</strong> January 17, 2000.<br />

This sample was tested <strong>on</strong> February 2, 2000. The examinati<strong>on</strong><br />

revealed an unheard-<str<strong>on</strong>g>of</str<strong>on</strong>g> AOC figure <str<strong>on</strong>g>of</str<strong>on</strong>g> <strong>on</strong>ly 1.8 µg<br />

carb<strong>on</strong> equivalent per litre, lower than ever before. Under<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g>se c<strong>on</strong>diti<strong>on</strong>s, bacterial growth is impossible and,<br />

as l<strong>on</strong>g as <str<strong>on</strong>g>the</str<strong>on</strong>g> bottle is not opened, <str<strong>on</strong>g>the</str<strong>on</strong>g> water will keep<br />

virtually forever. The explanati<strong>on</strong> for this extremely low<br />

AOC figure is pin point formati<strong>on</strong> induced by revitalisati<strong>on</strong>.<br />

The pin points have c<strong>on</strong>sumed almost all <str<strong>on</strong>g>the</str<strong>on</strong>g> nutrients<br />

in <str<strong>on</strong>g>the</str<strong>on</strong>g> water, so that <str<strong>on</strong>g>the</str<strong>on</strong>g> bottled Original Grander ®<br />

<str<strong>on</strong>g>Water</str<strong>on</strong>g> no l<strong>on</strong>ger tends <str<strong>on</strong>g>to</str<strong>on</strong>g>wards bacterial growth. The<br />

stability tests are now c<strong>on</strong>tinuing in <str<strong>on</strong>g>the</str<strong>on</strong>g>ir sixth year.<br />

Original Grander ® water bottled in March 1994, although<br />

it has been kept at room temperature, remains bacteriologically<br />

pure, meeting all <str<strong>on</strong>g>the</str<strong>on</strong>g> parameters <str<strong>on</strong>g>of</str<strong>on</strong>g> chapter B<br />

17 <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> codex, “Table <str<strong>on</strong>g>Water</str<strong>on</strong>g>”.<br />

9


The Significance <str<strong>on</strong>g>of</str<strong>on</strong>g> <strong>Grander®</strong> Pin Point Formati<strong>on</strong> in Practical Applicati<strong>on</strong><br />

It is still impossible <str<strong>on</strong>g>to</str<strong>on</strong>g> foresee <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

ultimate significance <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ®<br />

pin point formati<strong>on</strong> in its final c<strong>on</strong>sequences.<br />

In any case, this technology<br />

makes it possible <str<strong>on</strong>g>to</str<strong>on</strong>g> bottle drinking water<br />

without <str<strong>on</strong>g>the</str<strong>on</strong>g> additi<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> chemicals<br />

and <str<strong>on</strong>g>to</str<strong>on</strong>g> s<str<strong>on</strong>g>to</str<strong>on</strong>g>re it for years without loss<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> quality. Enormous drinking-water<br />

reservoirs, set aside for times <str<strong>on</strong>g>of</str<strong>on</strong>g> emergency,<br />

could be preserved in a bacteriologically<br />

stable state without <str<strong>on</strong>g>the</str<strong>on</strong>g> use<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> chemical additives. The same applies<br />

<str<strong>on</strong>g>to</str<strong>on</strong>g> drinking-water supplies that<br />

are maintained for use aboard ships,<br />

planes, caravans or dining-cars.<br />

The implementati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander<br />

® <strong>Technology</strong> at public swimming-pools<br />

has dem<strong>on</strong>strated that<br />

pin points show substantially more<br />

sensitivity <str<strong>on</strong>g>to</str<strong>on</strong>g> chlorine than do “wild”<br />

bacteria. Thus it is possible <str<strong>on</strong>g>to</str<strong>on</strong>g> safely<br />

reduce <str<strong>on</strong>g>the</str<strong>on</strong>g> chlorine c<strong>on</strong>tent <str<strong>on</strong>g>of</str<strong>on</strong>g> pool<br />

water <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> minimum limit <str<strong>on</strong>g>of</str<strong>on</strong>g> .3 mg<br />

chlorine per litre required by swimming-pool<br />

regulati<strong>on</strong>s.<br />

The implementati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> this technology<br />

in <str<strong>on</strong>g>the</str<strong>on</strong>g> field <str<strong>on</strong>g>of</str<strong>on</strong>g> rainwater-recovery<br />

systems has led <str<strong>on</strong>g>to</str<strong>on</strong>g> a reducti<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

<str<strong>on</strong>g>of</str<strong>on</strong>g>fensive odours and <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> settling<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> particles suspended in <str<strong>on</strong>g>the</str<strong>on</strong>g> water.<br />

Above all, Grander ® pin points are<br />

highly capable <str<strong>on</strong>g>of</str<strong>on</strong>g> reducing unpleasant<br />

odours, <str<strong>on</strong>g>the</str<strong>on</strong>g> cause <str<strong>on</strong>g>of</str<strong>on</strong>g> which is usually<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> activity <str<strong>on</strong>g>of</str<strong>on</strong>g> anaerobic bacteria.<br />

Evidently, oxygen c<strong>on</strong>diti<strong>on</strong>s are improved<br />

by structural changes in <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

water following revitalisati<strong>on</strong> with<br />

Grander ® <strong>Technology</strong>, thus eliminating<br />

anaerobic c<strong>on</strong>diti<strong>on</strong>s.<br />

In principle, a good source <str<strong>on</strong>g>of</str<strong>on</strong>g> drinking water is also<br />

characterised by a low water temperature, which ideally<br />

lies between five and eight degrees Celsius. Because <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

this low temperature <str<strong>on</strong>g>the</str<strong>on</strong>g> few bacteria found in this water<br />

almost appear <str<strong>on</strong>g>to</str<strong>on</strong>g> be dead. Of note is <str<strong>on</strong>g>the</str<strong>on</strong>g> sharp increase in<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g>ir reproductive period, <str<strong>on</strong>g>the</str<strong>on</strong>g> time a mo<str<strong>on</strong>g>the</str<strong>on</strong>g>r cell takes <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

divide in<str<strong>on</strong>g>to</str<strong>on</strong>g> two daughter cells. Under ideal circumstances<br />

– growing <strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> best culture medium and at room temperature<br />

– this reproductive period takes about 20 minutes.<br />

In <str<strong>on</strong>g>the</str<strong>on</strong>g> case <str<strong>on</strong>g>of</str<strong>on</strong>g> drinking water at a temperature <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

six degrees Celsius <str<strong>on</strong>g>the</str<strong>on</strong>g> reproductive period increases <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

around 10 <str<strong>on</strong>g>to</str<strong>on</strong>g> 12 hours. The bacteria become more active<br />

<strong>on</strong>ly after <str<strong>on</strong>g>the</str<strong>on</strong>g> water has been warmed <str<strong>on</strong>g>to</str<strong>on</strong>g> room temperature.<br />

The uniqueness <str<strong>on</strong>g>of</str<strong>on</strong>g> Original Grander ® <str<strong>on</strong>g>Water</str<strong>on</strong>g> lies in <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

fact that it is possible <str<strong>on</strong>g>to</str<strong>on</strong>g> s<str<strong>on</strong>g>to</str<strong>on</strong>g>re this bottled water without<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> additi<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> preservatives (i.e. carb<strong>on</strong>ic acid) for many<br />

years at room temperature, and it remains fit for c<strong>on</strong>sumpti<strong>on</strong>.<br />

C<strong>on</strong>tact Revitalisati<strong>on</strong> Using<br />

Grander ® <strong>Technology</strong><br />

Earlier we talked about a bottle c<strong>on</strong>taining Original<br />

Grander ® <str<strong>on</strong>g>Water</str<strong>on</strong>g> being placed next <str<strong>on</strong>g>to</str<strong>on</strong>g> a sealed bottle <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

water that had not been revitalised and <str<strong>on</strong>g>the</str<strong>on</strong>g> fact that this<br />

c<strong>on</strong>tact brought about revitalisati<strong>on</strong> after <strong>on</strong>ly a few days.<br />

Pin point formati<strong>on</strong> was found in <str<strong>on</strong>g>the</str<strong>on</strong>g> water that had originally<br />

not been revitalised. Our experience is that c<strong>on</strong>tact<br />

with Original Grander ® <str<strong>on</strong>g>Water</str<strong>on</strong>g> brings about revitalisati<strong>on</strong><br />

in a lessened, diluted form.<br />

This c<strong>on</strong>tact-revitalisati<strong>on</strong> represents <str<strong>on</strong>g>the</str<strong>on</strong>g> elemental<br />

and fundamental basic principle <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> mode <str<strong>on</strong>g>of</str<strong>on</strong>g> acti<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

Grander ® revitalisati<strong>on</strong> equipment and all o<str<strong>on</strong>g>the</str<strong>on</strong>g>r Grander ®<br />

products. Above all, bottling water using Grander ® revitalisati<strong>on</strong><br />

equipment is far more effective in its functi<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

transmitting informati<strong>on</strong> across wide distances and large<br />

surfaces. This high revitalisati<strong>on</strong> effect is also necessary,<br />

c<strong>on</strong>sidering that tap-water <str<strong>on</strong>g>of</str<strong>on</strong>g>ten passes through <str<strong>on</strong>g>the</str<strong>on</strong>g> equipment<br />

in <strong>on</strong>ly a fracti<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> a sec<strong>on</strong>d.<br />

For test purposes, we have removed Grander ® revitalisati<strong>on</strong><br />

equipment from water lines, and with <str<strong>on</strong>g>the</str<strong>on</strong>g> help<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> pin point method we tested how l<strong>on</strong>g it would take<br />

before <str<strong>on</strong>g>the</str<strong>on</strong>g> level <str<strong>on</strong>g>of</str<strong>on</strong>g> revitalisati<strong>on</strong> dropped. Revitalisati<strong>on</strong><br />

slows down <strong>on</strong>ly after a period <str<strong>on</strong>g>of</str<strong>on</strong>g> <strong>on</strong>e <str<strong>on</strong>g>to</str<strong>on</strong>g> two m<strong>on</strong>ths.<br />

Evidently informati<strong>on</strong> is s<str<strong>on</strong>g>to</str<strong>on</strong>g>red throughout <str<strong>on</strong>g>the</str<strong>on</strong>g> entire<br />

drinking-water system – a functi<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> materials used<br />

in <str<strong>on</strong>g>the</str<strong>on</strong>g> pipework.<br />

This revitalisati<strong>on</strong> effect <str<strong>on</strong>g>of</str<strong>on</strong>g> Original Grander ® <strong>Technology</strong>,<br />

which also includes <str<strong>on</strong>g>the</str<strong>on</strong>g> surrounding area, sometimes<br />

creates difficulties in a research labora<str<strong>on</strong>g>to</str<strong>on</strong>g>ry – especially if<br />

unrevitalised water is needed for purposes <str<strong>on</strong>g>of</str<strong>on</strong>g> comparis<strong>on</strong> and<br />

much <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> glass equipment being used has already been<br />

revitalised through c<strong>on</strong>tact.<br />

Summary<br />

The bacteriological tests that have been performed were intended<br />

<str<strong>on</strong>g>to</str<strong>on</strong>g> dem<strong>on</strong>strate that <strong>Grander®</strong> <strong>Technology</strong> brings<br />

about a bacteriological alterati<strong>on</strong> in <str<strong>on</strong>g>the</str<strong>on</strong>g> water. Evidently,<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> “wild” bacteria react <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> changed c<strong>on</strong>diti<strong>on</strong>s brought<br />

about by <strong>Grander®</strong> <strong>Technology</strong> in <str<strong>on</strong>g>the</str<strong>on</strong>g> form <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> pin<br />

point formati<strong>on</strong> described here and reduce <str<strong>on</strong>g>the</str<strong>on</strong>g> nutrient<br />

c<strong>on</strong>tent <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> water. This leads <str<strong>on</strong>g>to</str<strong>on</strong>g> a comprehensive palette<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> new and interesting qualities in water that has been<br />

revitalised with <str<strong>on</strong>g>the</str<strong>on</strong>g> help <str<strong>on</strong>g>of</str<strong>on</strong>g> <strong>Grander®</strong> <strong>Technology</strong>.<br />

10


Biological Method for Proving <str<strong>on</strong>g>the</str<strong>on</strong>g> Positive Alterati<strong>on</strong><br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> Heavy Metal Informati<strong>on</strong> in <str<strong>on</strong>g>Water</str<strong>on</strong>g>, Using <strong>Grander®</strong> Revitalisati<strong>on</strong><br />

The Phosphorescent<br />

Bacteria Test<br />

Johann Grander has said: “Grander ® <strong>Technology</strong> works with natural energy. Because <str<strong>on</strong>g>of</str<strong>on</strong>g> this<br />

quality it is able <str<strong>on</strong>g>to</str<strong>on</strong>g> reverse <str<strong>on</strong>g>the</str<strong>on</strong>g> polarity <str<strong>on</strong>g>of</str<strong>on</strong>g> negative informati<strong>on</strong> so that it can no l<strong>on</strong>ger<br />

have a negative effect. It is possible <str<strong>on</strong>g>to</str<strong>on</strong>g> neutralise negative informati<strong>on</strong> <strong>on</strong>ly by using energy<br />

and informati<strong>on</strong> that have come from nature itself.” The present work is an attempt <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

provide microbiological pro<str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>of</str<strong>on</strong>g> Johann Grander’s statement.<br />

In searching for biological test models <str<strong>on</strong>g>to</str<strong>on</strong>g> explain <str<strong>on</strong>g>the</str<strong>on</strong>g> positive<br />

alterati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> pollutant informati<strong>on</strong> by Grander ®<br />

<strong>Technology</strong>, I c<strong>on</strong>centrated primarily <strong>on</strong> investigative<br />

methods that are generally accessible or whose procedures<br />

have been standardised. It was important <str<strong>on</strong>g>to</str<strong>on</strong>g> find a method<br />

that could be duplicated in any labora<str<strong>on</strong>g>to</str<strong>on</strong>g>ry furnished with<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> proper equipment. This test report is an excerpt from a<br />

more comprehensive investigati<strong>on</strong>. Details <str<strong>on</strong>g>of</str<strong>on</strong>g> my testing<br />

methods may be obtained from me at any time.<br />

The American researcher A. A. Bulich established that<br />

phosphorescent bacteria are extremely sensitive. Even in <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

presence <str<strong>on</strong>g>of</str<strong>on</strong>g> minute quantities <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>to</str<strong>on</strong>g>xic material <str<strong>on</strong>g>the</str<strong>on</strong>g> phosphorescent<br />

intensity is reduced. In 1979 it occurred <str<strong>on</strong>g>to</str<strong>on</strong>g> him that<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g>se bacteria might be used <str<strong>on</strong>g>to</str<strong>on</strong>g> determine <str<strong>on</strong>g>the</str<strong>on</strong>g> <str<strong>on</strong>g>to</str<strong>on</strong>g>xicity <str<strong>on</strong>g>of</str<strong>on</strong>g> polluted<br />

water. He succeeded in measuring <str<strong>on</strong>g>the</str<strong>on</strong>g> bioluminescence<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> bacteria.<br />

Summarised in a single paragraph, <str<strong>on</strong>g>the</str<strong>on</strong>g> principle <str<strong>on</strong>g>of</str<strong>on</strong>g> measurement<br />

seems very “enlightening”: <str<strong>on</strong>g>the</str<strong>on</strong>g> phosphorescent<br />

bacterium radiates with full intensity when it is doing well. But<br />

in <str<strong>on</strong>g>the</str<strong>on</strong>g> presence <str<strong>on</strong>g>of</str<strong>on</strong>g> even <str<strong>on</strong>g>the</str<strong>on</strong>g> smallest quantities <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>to</str<strong>on</strong>g>xic material<br />

it phosphoresces with diminished intensity. The difference in<br />

phosphorescent output can be measured.<br />

The Phosphorescent Bacteria Test<br />

– a Standardised Procedure<br />

In <str<strong>on</strong>g>the</str<strong>on</strong>g> meantime, <str<strong>on</strong>g>the</str<strong>on</strong>g> phosphorescent bacteria test has<br />

proved itself <str<strong>on</strong>g>to</str<strong>on</strong>g> such an extent that in 1991 <str<strong>on</strong>g>the</str<strong>on</strong>g> procedure was<br />

standardised. According <str<strong>on</strong>g>to</str<strong>on</strong>g> DIN 38412, Part 34, it is possible <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

test waste water using phosphorescent bacteria. Even though<br />

this norm was developed for pollutants in waste water, it is<br />

applicable <str<strong>on</strong>g>to</str<strong>on</strong>g> every o<str<strong>on</strong>g>the</str<strong>on</strong>g>r kind <str<strong>on</strong>g>of</str<strong>on</strong>g> water as well, including<br />

drinking water, and even drainage water from dumps.<br />

Material and Method<br />

The phosphorescent bacteria used for testing were obtained<br />

from <str<strong>on</strong>g>the</str<strong>on</strong>g> firm Dr. Lange (where <str<strong>on</strong>g>the</str<strong>on</strong>g>y are called LUMISmini).<br />

They are s<str<strong>on</strong>g>to</str<strong>on</strong>g>red at a temperature <str<strong>on</strong>g>of</str<strong>on</strong>g> -18 degrees Celsius. With<br />

11


Bioluminescence<br />

Every<strong>on</strong>e is familiar with <str<strong>on</strong>g>the</str<strong>on</strong>g> female firefly, who sets out<br />

at twilight <strong>on</strong> mild May evenings <str<strong>on</strong>g>to</str<strong>on</strong>g> attract male fireflies<br />

with her “lantern”. In some species, female fireflies<br />

(glow-worms) are incapable <str<strong>on</strong>g>of</str<strong>on</strong>g> flight. For that reas<strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g>y<br />

climb <strong>on</strong><str<strong>on</strong>g>to</str<strong>on</strong>g> blades <str<strong>on</strong>g>of</str<strong>on</strong>g> grass, sending out <str<strong>on</strong>g>the</str<strong>on</strong>g>ir characteristic<br />

greenish-yellow light. They do so in hope <str<strong>on</strong>g>of</str<strong>on</strong>g> attracting an<br />

airworthy male partner, who resp<strong>on</strong>ds with a light signal<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> his own.<br />

The aquatic phosphorescent bacteria <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> types called<br />

Pho<str<strong>on</strong>g>to</str<strong>on</strong>g>bacterium phosphoreum and Vibrio fischeri are found in<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> sea, where <str<strong>on</strong>g>the</str<strong>on</strong>g>y broadcast a bluish fluorescent light<br />

with a wavelength <str<strong>on</strong>g>of</str<strong>on</strong>g> 490 nanometres. This bioluminescence<br />

(light emissi<strong>on</strong> by an organism) is directly dependent<br />

<strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> metabolic (variable) c<strong>on</strong>diti<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> bacterial<br />

cells. The emissi<strong>on</strong>s are particularly str<strong>on</strong>g when <str<strong>on</strong>g>the</str<strong>on</strong>g>y<br />

are doing well.<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> reactivati<strong>on</strong> liquid, which is supplied at <str<strong>on</strong>g>the</str<strong>on</strong>g> same time,<br />

bacteria can be prepared for testing in <strong>on</strong>ly 15 minutes. Al<strong>on</strong>g<br />

with <str<strong>on</strong>g>the</str<strong>on</strong>g>se reactivated phosphorescent bacteria, tests are<br />

also performed <strong>on</strong> bacterial suspensi<strong>on</strong>s that are prepared<br />

in <str<strong>on</strong>g>the</str<strong>on</strong>g> labora<str<strong>on</strong>g>to</str<strong>on</strong>g>ry.<br />

This pho<str<strong>on</strong>g>to</str<strong>on</strong>g>graph <str<strong>on</strong>g>of</str<strong>on</strong>g> phosphorescent<br />

bacteria under a microscope shows<br />

that <str<strong>on</strong>g>the</str<strong>on</strong>g>re is luminescence outside<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> cell nucleus. The strength <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> bioluminescence is a measure<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> positive metabolic c<strong>on</strong>diti<strong>on</strong><br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> bacteria. Toxic substances reduce<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> phosphorescent output.<br />

In <str<strong>on</strong>g>the</str<strong>on</strong>g> dark, <str<strong>on</strong>g>the</str<strong>on</strong>g> phosphorescence<br />

(bioluminescence) <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> bacteria<br />

can be observed particularly clearly<br />

with <str<strong>on</strong>g>the</str<strong>on</strong>g> naked eye. With <str<strong>on</strong>g>the</str<strong>on</strong>g> additi<strong>on</strong><br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> appropriate amount <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

salt, phosphorescent bacteria can<br />

be cultured <strong>on</strong> labora<str<strong>on</strong>g>to</str<strong>on</strong>g>ry plates.<br />

That permits <str<strong>on</strong>g>the</str<strong>on</strong>g> producti<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

fresh bacteria suspensi<strong>on</strong>s that<br />

show a particularly high sensitivity<br />

<str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>to</str<strong>on</strong>g>xic substances.<br />

The Special Qualities <str<strong>on</strong>g>of</str<strong>on</strong>g> Phosphorescent Bacteria<br />

• Bacteria in general, and marine bacteria in particular, are<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> oldest known living creatures, and yet <str<strong>on</strong>g>the</str<strong>on</strong>g>y are evidently<br />

particularly sensitive <str<strong>on</strong>g>to</str<strong>on</strong>g> pollutant substances.<br />

• Phosphorescent bacteria usually react sensitively and<br />

quickly. This means that a measurement taken after <strong>on</strong>ly<br />

30 minutes <str<strong>on</strong>g>of</str<strong>on</strong>g>ten results in a definite c<strong>on</strong>clusi<strong>on</strong>.<br />

• Around <strong>on</strong>e milli<strong>on</strong> bacteria are used in a single phosphorescent<br />

bacteria test. This means <str<strong>on</strong>g>the</str<strong>on</strong>g> test examines <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

behaviour <str<strong>on</strong>g>of</str<strong>on</strong>g> <strong>on</strong>e milli<strong>on</strong> individual cells.<br />

• The phosphorescent bacteria test can be standardised<br />

using <str<strong>on</strong>g>the</str<strong>on</strong>g> methods <str<strong>on</strong>g>of</str<strong>on</strong>g> quality c<strong>on</strong>trol. This means that<br />

definite and comparable – and above all repeatable – results<br />

can be produced.<br />

Lab stati<strong>on</strong> for phosphorescent bacteria tests.<br />

In <str<strong>on</strong>g>the</str<strong>on</strong>g> foreground are two LUMISmini pho<str<strong>on</strong>g>to</str<strong>on</strong>g>meters, behind <str<strong>on</strong>g>the</str<strong>on</strong>g>m a<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g>rmoblock for maintaining <str<strong>on</strong>g>the</str<strong>on</strong>g> suspensi<strong>on</strong> at a fixed temperature.<br />

Measurements are taken in disposable glass bowls at<br />

15 degrees Celsius and at 21 degrees Celsius.<br />

The luminescence measurements are c<strong>on</strong>ducted using a<br />

measuring device from <str<strong>on</strong>g>the</str<strong>on</strong>g> firm Dr. Lange called LUMISmini<br />

Type LPG 298. Two kinds <str<strong>on</strong>g>of</str<strong>on</strong>g> measurement can be recorded<br />

with this device: <str<strong>on</strong>g>the</str<strong>on</strong>g> actual phosphorescent output, or <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

variati<strong>on</strong> (increase or reducti<strong>on</strong>) in phosphorescent output<br />

relative <str<strong>on</strong>g>to</str<strong>on</strong>g> a pre-selected standard value. A single measurement<br />

takes about 10 sec<strong>on</strong>ds. In every experiment a standard<br />

sample c<strong>on</strong>sisting <str<strong>on</strong>g>of</str<strong>on</strong>g> unrevitalised natural drinking water<br />

c<strong>on</strong>taining 2 per cent sodium chloride is also tested.<br />

Detail <str<strong>on</strong>g>of</str<strong>on</strong>g> a LUMISmeter with which <str<strong>on</strong>g>the</str<strong>on</strong>g> phosphorescent output<br />

(bioluminescence) <str<strong>on</strong>g>of</str<strong>on</strong>g> a phosphorescent bacteria suspensi<strong>on</strong> can be<br />

measured. The instrument records <str<strong>on</strong>g>the</str<strong>on</strong>g> reducti<strong>on</strong> in phosphorescent<br />

output as a percentage compared with a standard value.<br />

12


The Test Method<br />

In this series <str<strong>on</strong>g>of</str<strong>on</strong>g> tests, unpolluted, n<strong>on</strong>-revitalised drinking<br />

water is c<strong>on</strong>taminated with heavy metals. Since <str<strong>on</strong>g>the</str<strong>on</strong>g>se<br />

phosphorescent bacteria are marine creatures, it is necessary<br />

<str<strong>on</strong>g>to</str<strong>on</strong>g> add 2 per cent sodium <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> water being tested. This<br />

water sample is <str<strong>on</strong>g>the</str<strong>on</strong>g>n divided in<str<strong>on</strong>g>to</str<strong>on</strong>g> two equal parts. One part<br />

is revitalised by dipping a Grander ® Energy Rod in<str<strong>on</strong>g>to</str<strong>on</strong>g> it for<br />

about 10 sec<strong>on</strong>ds; <str<strong>on</strong>g>the</str<strong>on</strong>g> sec<strong>on</strong>d part remains unrevitalised.<br />

Thus <str<strong>on</strong>g>the</str<strong>on</strong>g> compositi<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> both water samples is completely<br />

identical. Revitalisati<strong>on</strong> with a Grander ® Energy Rod (filled<br />

with Grander ® Informati<strong>on</strong> <str<strong>on</strong>g>Water</str<strong>on</strong>g>) nei<str<strong>on</strong>g>the</str<strong>on</strong>g>r adds anything<br />

<str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> water nor removes anything from it. Both water<br />

samples are <str<strong>on</strong>g>the</str<strong>on</strong>g>n subjected <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> phosphorescent bacteria<br />

test. The bacterial suspensi<strong>on</strong> used for <str<strong>on</strong>g>the</str<strong>on</strong>g> test is distributed<br />

in <str<strong>on</strong>g>the</str<strong>on</strong>g> same quantity and c<strong>on</strong>centrati<strong>on</strong> in both test series,<br />

and <str<strong>on</strong>g>the</str<strong>on</strong>g> phosphorescent output is measured at <str<strong>on</strong>g>the</str<strong>on</strong>g> same<br />

temperature and after <str<strong>on</strong>g>the</str<strong>on</strong>g> same period <str<strong>on</strong>g>of</str<strong>on</strong>g> time. This is <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

basic c<strong>on</strong>cept for <str<strong>on</strong>g>the</str<strong>on</strong>g> test series.<br />

Phosphorescent Bacteria Test <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

<str<strong>on</strong>g>Water</str<strong>on</strong>g> with Lead C<strong>on</strong>centrati<strong>on</strong>s<br />

between .0625 and 1 mg/l<br />

N<strong>on</strong>-revitalised drinking water with a lead c<strong>on</strong>tent below<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> analytically detectable level <str<strong>on</strong>g>of</str<strong>on</strong>g> 0.01 mg/l l was mixed in <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

labora<str<strong>on</strong>g>to</str<strong>on</strong>g>ry with lead salt (lead nitrate), so that <str<strong>on</strong>g>the</str<strong>on</strong>g> following<br />

c<strong>on</strong>centrati<strong>on</strong>s were achieved: 0.0625 mg/l, 0.125 mg/l, 0.25<br />

mg/l, 0.5 mg/l and 1.0 mg/l.<br />

The highest c<strong>on</strong>centrati<strong>on</strong> permitted in drinking water<br />

lies at present at around 0.05 mg/l. Thus <str<strong>on</strong>g>the</str<strong>on</strong>g> water samples<br />

tested here would have been, with <str<strong>on</strong>g>the</str<strong>on</strong>g> excepti<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> first<br />

sample, c<strong>on</strong>sidered unpotable.<br />

Results<br />

The beams below <str<strong>on</strong>g>the</str<strong>on</strong>g> 0%-line <strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> ordinate represent<br />

inhibiti<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> phosphorescent output, <str<strong>on</strong>g>the</str<strong>on</strong>g> beams above<br />

an increase in phosphorescent output – a result <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ®<br />

revitalisati<strong>on</strong>. These increases in phosphorescent output occur<br />

in c<strong>on</strong>centrati<strong>on</strong>s below 0.25 mg lead per litre <str<strong>on</strong>g>of</str<strong>on</strong>g> drinking water.<br />

Evidently, this is a catalytic effect <strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> biological process<br />

resp<strong>on</strong>sible for phosphorescent output.<br />

The chart indicates <str<strong>on</strong>g>the</str<strong>on</strong>g> effect <strong>on</strong> n<strong>on</strong>-revitalised water<br />

after a period <str<strong>on</strong>g>of</str<strong>on</strong>g> 30 minutes. A c<strong>on</strong>centrati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> 0.0625 mg<br />

lead per litre <str<strong>on</strong>g>of</str<strong>on</strong>g> drinking water experiences no significant<br />

reducti<strong>on</strong> in phosphorescent output. At a c<strong>on</strong>centrati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

0.125 mg lead per litre <str<strong>on</strong>g>the</str<strong>on</strong>g> reducti<strong>on</strong> is 6%, while at a c<strong>on</strong>centrati<strong>on</strong><br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> 0.25 mg lead per litre <str<strong>on</strong>g>the</str<strong>on</strong>g>re is a 10% reducti<strong>on</strong>. At<br />

c<strong>on</strong>centrati<strong>on</strong>s <str<strong>on</strong>g>of</str<strong>on</strong>g> 0.5 mg lead per litre <str<strong>on</strong>g>the</str<strong>on</strong>g> reducti<strong>on</strong> is 33%,<br />

and at a c<strong>on</strong>centrati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> 1.0 mg lead per litre it is 52%.<br />

After a wait <str<strong>on</strong>g>of</str<strong>on</strong>g> 60 minutes, <str<strong>on</strong>g>the</str<strong>on</strong>g> comparative values are -3%,<br />

-5%, -19%, -43% and -62%.<br />

The chart also indicates <str<strong>on</strong>g>the</str<strong>on</strong>g> effect <strong>on</strong> Grander ® revitalised<br />

water after a period <str<strong>on</strong>g>of</str<strong>on</strong>g> 30 minutes. At a c<strong>on</strong>centrati<strong>on</strong><br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> 0.0625 mg per litre <str<strong>on</strong>g>of</str<strong>on</strong>g> drinking water, <str<strong>on</strong>g>the</str<strong>on</strong>g>re is an actual<br />

increase in phosphorescent output <str<strong>on</strong>g>of</str<strong>on</strong>g> 32%. At a c<strong>on</strong>centrati<strong>on</strong><br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> 0.125 mg/l <str<strong>on</strong>g>the</str<strong>on</strong>g> increase is 14%, and at 0.25 mg/l<br />

Lead in Drinking <str<strong>on</strong>g>Water</str<strong>on</strong>g><br />

30-minute durati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> acti<strong>on</strong> – freshly cultured phosphorescent bacteria<br />

Grander ® revitalised<br />

unrevitalised<br />

reducti<strong>on</strong> or increase in %<br />

40%<br />

30%<br />

20%<br />

10%<br />

0%<br />

10%<br />

32%<br />

0%<br />

14%<br />

6%<br />

7%<br />

10%<br />

1%<br />

20%<br />

17%<br />

30%<br />

40%<br />

50%<br />

60%<br />

0.0625 mg/l 0.125 mg/l 0.25 mg/l 0.5 mg/l 1.0 mg/l<br />

33%<br />

52%<br />

C<strong>on</strong>centrati<strong>on</strong>s<br />

13


Lead in Drinking <str<strong>on</strong>g>Water</str<strong>on</strong>g><br />

60-minute durati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> acti<strong>on</strong> – freshly cultured phosphorescent bacteria<br />

Grander ® revitalised<br />

unrevitalised<br />

reducti<strong>on</strong> or increase in %<br />

20%<br />

10%<br />

0%<br />

10%<br />

20%<br />

30%<br />

10%<br />

3%<br />

1%<br />

5% 5%<br />

19%<br />

15%<br />

40%<br />

50%<br />

60%<br />

70%<br />

43% 44%<br />

0.0625 mg/l 0.125 mg/l 0.25 mg/l 0.5 mg/l 1.0 mg/l<br />

62%<br />

C<strong>on</strong>centrati<strong>on</strong>s<br />

around 7%. A small reducti<strong>on</strong> occurs at a lead c<strong>on</strong>centrati<strong>on</strong><br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> 0.5 mg/l with -1%; at a c<strong>on</strong>centrati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> 1.0 mg/l <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

reducti<strong>on</strong> is -17%.<br />

After a wait <str<strong>on</strong>g>of</str<strong>on</strong>g> 60 minutes, <str<strong>on</strong>g>the</str<strong>on</strong>g> comparative values are<br />

+10%, +1%, -5%, -15% and -44%<br />

Significant Differences<br />

<str<strong>on</strong>g>to</str<strong>on</strong>g> N<strong>on</strong>-revitalised <str<strong>on</strong>g>Water</str<strong>on</strong>g><br />

These test results show significant differences between<br />

water revitalised with Grander ® and n<strong>on</strong>-revitalised water<br />

when both c<strong>on</strong>tain similar c<strong>on</strong>centrati<strong>on</strong>s <str<strong>on</strong>g>of</str<strong>on</strong>g> lead.<br />

The values reached after 30 minutes are particularly interesting,<br />

while those reached after 60 minutes do not represent<br />

as significant a difference. This, however, does not mean that<br />

Grander ® <strong>Technology</strong> is less effective, but ra<str<strong>on</strong>g>the</str<strong>on</strong>g>r that <str<strong>on</strong>g>the</str<strong>on</strong>g> effect<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> pois<strong>on</strong> is time dependent.<br />

In lead-c<strong>on</strong>taining water that has been revitalised using<br />

Grander ® technology, after 30 minutes c<strong>on</strong>centrati<strong>on</strong>s <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

up <str<strong>on</strong>g>to</str<strong>on</strong>g> 0.5 mg lead per litre <str<strong>on</strong>g>of</str<strong>on</strong>g> drinking water show practically<br />

no reducti<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> phosphorescent output. Not until a<br />

value <str<strong>on</strong>g>of</str<strong>on</strong>g> 1.0 mg per litre is an effect <str<strong>on</strong>g>of</str<strong>on</strong>g> -17% recorded. In<br />

n<strong>on</strong>-revitalised water a reducti<strong>on</strong> is already recorded at a<br />

c<strong>on</strong>centrati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> 0.125 mg per litre and reaches -52% at 1.0<br />

mg per litre.<br />

Phosphorescent Bacteria Tests<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>Water</str<strong>on</strong>g> with Mercury C<strong>on</strong>tent<br />

between 0.0007 and 0.07 mg/l<br />

N<strong>on</strong>-revitalised drinking water with a mercury c<strong>on</strong>tent<br />

below <str<strong>on</strong>g>the</str<strong>on</strong>g> analytically detectable level <str<strong>on</strong>g>of</str<strong>on</strong>g> .0005 mg/l was<br />

mixed with a mercury salt (mercury II sulphate) so that <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

following c<strong>on</strong>centrati<strong>on</strong>s were achieved: 0.0007 mg/l, 0.0035<br />

mg/l, 0.007 mg/l, 0.035 mg/l and 0.07 mg/l. The highest c<strong>on</strong>centrati<strong>on</strong><br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> mercury currently permitted in drinking water<br />

Results in Samples C<strong>on</strong>taining Mercury<br />

0.0007 mg Hg/l 0.0035 mg Hg/l 0.007 Hg/l 0.035 mg Hg/l 0.07 mg Hg/l<br />

unrevitalised 0% –10% –18% –51% –89%<br />

Grander ® revitalised -42% +28% +17% +13% –35%<br />

50-minute durati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> acti<strong>on</strong> – freshly cultured phosphorescent bacteria (1 ml sample + 0.4 ml bacterial suspensi<strong>on</strong>).<br />

14


Mercury in Drinking <str<strong>on</strong>g>Water</str<strong>on</strong>g><br />

50-minute durati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> acti<strong>on</strong> – freshly cultured phosphorescent bacteria<br />

Grander ® revitalised<br />

unrevitalised<br />

reducti<strong>on</strong> or increase in %<br />

60%<br />

40%<br />

20%<br />

0%<br />

20%<br />

40%<br />

42%<br />

0%<br />

28%<br />

10%<br />

17%<br />

18%<br />

13%<br />

35%<br />

60%<br />

51%<br />

80%<br />

100%<br />

0.0007 mg/l 0.0035 mg/l 0.007 mg/l 0.035 mg/l 0.07 mg/l<br />

89%<br />

C<strong>on</strong>centrati<strong>on</strong>s<br />

is 0.001 mg/l. Thus <str<strong>on</strong>g>the</str<strong>on</strong>g> water samples that were tested were,<br />

with <str<strong>on</strong>g>the</str<strong>on</strong>g> excepti<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> first sample, were n<strong>on</strong>potable.<br />

Results<br />

<str<strong>on</strong>g>An</str<strong>on</strong>g> increase in <str<strong>on</strong>g>the</str<strong>on</strong>g> phosphorescent output <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ® -<br />

revitalised water samples was found at low c<strong>on</strong>centrati<strong>on</strong>s <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

lead and cadmium as well The chart shows <str<strong>on</strong>g>the</str<strong>on</strong>g> results after 50<br />

minutes. Compared with lead, mercury is substantially more<br />

<str<strong>on</strong>g>to</str<strong>on</strong>g>xic <str<strong>on</strong>g>to</str<strong>on</strong>g> phosphorescent bacteria, or <str<strong>on</strong>g>to</str<strong>on</strong>g> reverse <str<strong>on</strong>g>the</str<strong>on</strong>g> statement:<br />

phosphorescent bacteria are substantially more sensitive <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

mercury in soluti<strong>on</strong> than <str<strong>on</strong>g>the</str<strong>on</strong>g>y are <str<strong>on</strong>g>to</str<strong>on</strong>g> lead.<br />

In <str<strong>on</strong>g>the</str<strong>on</strong>g> sample that had underg<strong>on</strong>e Grander ® revitalisati<strong>on</strong>,<br />

an increase in phosphorescent output was seen in c<strong>on</strong>centrati<strong>on</strong>s<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> up <str<strong>on</strong>g>to</str<strong>on</strong>g> .035 mg/l. Not until c<strong>on</strong>centrati<strong>on</strong>s <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

0.07 mg/l and higher was <str<strong>on</strong>g>the</str<strong>on</strong>g>re a reducti<strong>on</strong> in phosphorescent<br />

output <str<strong>on</strong>g>of</str<strong>on</strong>g> around -35% in <str<strong>on</strong>g>the</str<strong>on</strong>g> revitalised sample as well.<br />

In <str<strong>on</strong>g>the</str<strong>on</strong>g> unrevitalised water c<strong>on</strong>taining mercury <str<strong>on</strong>g>the</str<strong>on</strong>g>re<br />

was a small reducti<strong>on</strong> already at 0.0035 mg/l. The biggest<br />

difference between <str<strong>on</strong>g>the</str<strong>on</strong>g> Grander ® revitalised sample and <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

unrevitalised sample was at a c<strong>on</strong>centrati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> 0.035 mg/l.<br />

<str<strong>on</strong>g>An</str<strong>on</strong>g> increase in phosphorescent output <str<strong>on</strong>g>of</str<strong>on</strong>g> around +13% was<br />

seen in <str<strong>on</strong>g>the</str<strong>on</strong>g> revitalised sample; in <str<strong>on</strong>g>the</str<strong>on</strong>g> unrevitalised sample <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

reducti<strong>on</strong> was -51%.<br />

Phosphorescent Bacteria Test<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>Water</str<strong>on</strong>g> with Cadmium<br />

C<strong>on</strong>centrati<strong>on</strong>s between<br />

0.005 and 0.25 mg/l<br />

<str<strong>on</strong>g>An</str<strong>on</strong>g>alogously <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> previous tests, cadmium-free drinking<br />

water was mixed with cadmium i<strong>on</strong>s in <str<strong>on</strong>g>the</str<strong>on</strong>g> form <str<strong>on</strong>g>of</str<strong>on</strong>g> cadmium<br />

chloride m<strong>on</strong>ohydrate, so that <str<strong>on</strong>g>the</str<strong>on</strong>g> c<strong>on</strong>centrati<strong>on</strong>s 0.005 mg/l,<br />

0.01 mg/l, 0.05 mg/l, 0.1 mg/l and 0.25 mg/l were reached. The<br />

present maximum permissible c<strong>on</strong>centrati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> cadmium in<br />

drinking water is 0.005 mg/l.<br />

Results in Samples C<strong>on</strong>taining Cadmium<br />

0.005 mg Cd/l 0.01 mg Cd/l 0.05 Cd/l 0.1 mg Cd/l 0.25 mg Cd/l<br />

unrevitalised 11% –20% –31% –38% –53%<br />

Grander ® revitalised +21% +11% –7% –20% –47%<br />

60-minute durati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> acti<strong>on</strong> – freshly cultured phosphorescent bacteria (1 ml sample + 0.4 ml bacterial suspensi<strong>on</strong>).<br />

15


Cadmium in Drinking <str<strong>on</strong>g>Water</str<strong>on</strong>g><br />

60-minute durati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> acti<strong>on</strong> – freshly cultured phosphorescent bacteria<br />

Grander ® revitalised<br />

unrevitalised<br />

reducti<strong>on</strong> or increase in %<br />

30%<br />

20%<br />

10%<br />

0%<br />

10%<br />

20%<br />

21%<br />

11%<br />

11%<br />

20%<br />

7%<br />

20%<br />

30%<br />

40%<br />

31%<br />

38%<br />

50%<br />

60%<br />

0.005 mg/l 0.01 mg/l 0.05 mg/l 0.1 mg/l 0.25 mg/l<br />

47%<br />

53%<br />

C<strong>on</strong>centrati<strong>on</strong>s<br />

Results<br />

The phosphorescent bacteria display a sensitivity <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

cadmium i<strong>on</strong>s that is greater than in <str<strong>on</strong>g>the</str<strong>on</strong>g> case <str<strong>on</strong>g>of</str<strong>on</strong>g> lead i<strong>on</strong>s<br />

but not as high as in <str<strong>on</strong>g>the</str<strong>on</strong>g> case <str<strong>on</strong>g>of</str<strong>on</strong>g> mercury i<strong>on</strong>s. The greatest<br />

difference between Grander ® -revitalised and unrevitalised<br />

water c<strong>on</strong>taining cadmium is evident at c<strong>on</strong>centrati<strong>on</strong>s <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

0.005 mg/l, 0.01 mg/l and 0.05 mg/l. No significant difference<br />

between <str<strong>on</strong>g>the</str<strong>on</strong>g> Grander ® -revitalised and unrevitalised<br />

samples was measured at 0.25 mg/l. This c<strong>on</strong>centrati<strong>on</strong> is<br />

<str<strong>on</strong>g>to</str<strong>on</strong>g>o high for Grander ® revitalisati<strong>on</strong> <str<strong>on</strong>g>to</str<strong>on</strong>g> result in a reducti<strong>on</strong><br />

in <str<strong>on</strong>g>to</str<strong>on</strong>g>xicity <str<strong>on</strong>g>to</str<strong>on</strong>g> phosphorescent bacteria.<br />

The Statistics <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

<str<strong>on</strong>g>An</str<strong>on</strong>g>alysis Method<br />

Ring tests reveal that <str<strong>on</strong>g>the</str<strong>on</strong>g> coefficient <str<strong>on</strong>g>of</str<strong>on</strong>g> variati<strong>on</strong> –<br />

depending <strong>on</strong> what is being measured – varies between<br />

6% and 16%. In measuring <str<strong>on</strong>g>the</str<strong>on</strong>g> same heavy metals and in 10<br />

individual measurements under completely identical c<strong>on</strong>diti<strong>on</strong>s<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> deviati<strong>on</strong> was <strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> order <str<strong>on</strong>g>of</str<strong>on</strong>g> ±5%.<br />

In <str<strong>on</strong>g>the</str<strong>on</strong>g> phosphorescent bacteria test it has become established<br />

practice that reducti<strong>on</strong>s amounting <str<strong>on</strong>g>to</str<strong>on</strong>g> less than 20%<br />

are not <str<strong>on</strong>g>the</str<strong>on</strong>g> subject <str<strong>on</strong>g>of</str<strong>on</strong>g> fur<str<strong>on</strong>g>the</str<strong>on</strong>g>r discussi<strong>on</strong>. In such cases <str<strong>on</strong>g>the</str<strong>on</strong>g> test<br />

medium is c<strong>on</strong>sidered <str<strong>on</strong>g>to</str<strong>on</strong>g> be <str<strong>on</strong>g>of</str<strong>on</strong>g> low <str<strong>on</strong>g>to</str<strong>on</strong>g>xicity. This is also taken<br />

in<str<strong>on</strong>g>to</str<strong>on</strong>g> account in <str<strong>on</strong>g>the</str<strong>on</strong>g> test results presented here. Specifically:<br />

differences <str<strong>on</strong>g>of</str<strong>on</strong>g> less than 20% are not c<strong>on</strong>sidered <str<strong>on</strong>g>to</str<strong>on</strong>g> be particularly<br />

meaningful.<br />

What Is <str<strong>on</strong>g>the</str<strong>on</strong>g> Meaning <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

Test Results and How Should<br />

They Be Used?<br />

The three “most hazardous” heavy metals in drinking<br />

water (lead, cadmium and mercury) were examined in <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

Reducti<strong>on</strong> in Phosphorescent Output according <str<strong>on</strong>g>to</str<strong>on</strong>g> Koller-Kreiml and Rodinger, 1987<br />

% <str<strong>on</strong>g>of</str<strong>on</strong>g> reducti<strong>on</strong> after 30 minutes durati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> acti<strong>on</strong> Assessment<br />

under 10%<br />

insignificant reducti<strong>on</strong><br />

10% - 40% moderately str<strong>on</strong>g reducti<strong>on</strong><br />

40% - 60% str<strong>on</strong>g reducti<strong>on</strong><br />

60% - 90% very str<strong>on</strong>g reducti<strong>on</strong><br />

over 90%<br />

complete eliminati<strong>on</strong><br />

16


phosphorescent bacteria test and significant reducti<strong>on</strong> differences<br />

between unrevitalised and Grander ® -revitalised water<br />

were established. After eliminati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> all sources <str<strong>on</strong>g>of</str<strong>on</strong>g> error,<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g>se differences were attributed <str<strong>on</strong>g>to</str<strong>on</strong>g> a positive informati<strong>on</strong><br />

change caused by Grander ® <strong>Technology</strong>.<br />

By no means should <str<strong>on</strong>g>the</str<strong>on</strong>g>se first results be used <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

promote Grander ® <strong>Technology</strong> as a method for lowering<br />

“heavy metal polluti<strong>on</strong>”. Since Grander ® revitalisati<strong>on</strong><br />

equipment is used in <str<strong>on</strong>g>the</str<strong>on</strong>g> field <str<strong>on</strong>g>of</str<strong>on</strong>g> drinking water <strong>on</strong>ly when<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> water is potable, <str<strong>on</strong>g>the</str<strong>on</strong>g>re is no fundamental reas<strong>on</strong> <str<strong>on</strong>g>to</str<strong>on</strong>g> even<br />

c<strong>on</strong>sider it for such a use.<br />

The phosphorescent bacteria test is an interesting biological<br />

test model for establishing informati<strong>on</strong> changes. Many<br />

additi<strong>on</strong>al tests will be necessary in future. For when a positive<br />

informati<strong>on</strong> change has been established, this change will<br />

apply not <strong>on</strong>ly <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> heavy metal c<strong>on</strong>tent <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> drinking<br />

water but will be generally applicable <str<strong>on</strong>g>to</str<strong>on</strong>g> all o<str<strong>on</strong>g>the</str<strong>on</strong>g>r c<strong>on</strong>tents<br />

within a certain level <str<strong>on</strong>g>of</str<strong>on</strong>g> c<strong>on</strong>centrati<strong>on</strong>.<br />

For example, when nitrate c<strong>on</strong>centrati<strong>on</strong>s <str<strong>on</strong>g>of</str<strong>on</strong>g> up <str<strong>on</strong>g>to</str<strong>on</strong>g> 500<br />

mg/l were tested, <str<strong>on</strong>g>the</str<strong>on</strong>g> phosphorescent bacteria always reacted<br />

with an increase in phosphorescent output. Nitrates are evidently<br />

food sources for phosphorescent bacteria. Nitrates are<br />

also ubiqui<str<strong>on</strong>g>to</str<strong>on</strong>g>us in seawater. Thus this result should come as<br />

no surprise.<br />

Summary<br />

With <str<strong>on</strong>g>the</str<strong>on</strong>g> help <str<strong>on</strong>g>of</str<strong>on</strong>g> phosphorescent bacteria tests standardised<br />

according <str<strong>on</strong>g>to</str<strong>on</strong>g> DIN, this highly sensitive biological model<br />

was used for <str<strong>on</strong>g>the</str<strong>on</strong>g> first time <str<strong>on</strong>g>to</str<strong>on</strong>g> examine <str<strong>on</strong>g>the</str<strong>on</strong>g> questi<strong>on</strong>:<br />

Can a positive alterati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> pollutant informati<strong>on</strong> as a<br />

direct result <str<strong>on</strong>g>of</str<strong>on</strong>g> using Grander ® <strong>Technology</strong> be scientifically<br />

established?<br />

In <str<strong>on</strong>g>the</str<strong>on</strong>g> case <str<strong>on</strong>g>of</str<strong>on</strong>g> drinking water samples that were artificially<br />

polluted with lead, mercury and cadmium, <str<strong>on</strong>g>the</str<strong>on</strong>g> inhibiti<strong>on</strong><br />

was significantly smaller in <str<strong>on</strong>g>the</str<strong>on</strong>g> Grander ® -revitalised water<br />

at lower c<strong>on</strong>centrati<strong>on</strong>s than in unrevitalised water.<br />

Despite <str<strong>on</strong>g>the</str<strong>on</strong>g>se results, however, Grander ® <strong>Technology</strong><br />

should not be used in an attempt <str<strong>on</strong>g>to</str<strong>on</strong>g> remove <str<strong>on</strong>g>to</str<strong>on</strong>g>xins from<br />

water c<strong>on</strong>taining heavy metals.<br />

The experiment c<strong>on</strong>firmed that phosphorescent bacteria<br />

are well suited for use in such examinati<strong>on</strong>s. The research<br />

work will be extended <str<strong>on</strong>g>to</str<strong>on</strong>g> a large number <str<strong>on</strong>g>of</str<strong>on</strong>g> o<str<strong>on</strong>g>the</str<strong>on</strong>g>r pollutants<br />

found in drinking water in order <str<strong>on</strong>g>to</str<strong>on</strong>g> fur<str<strong>on</strong>g>the</str<strong>on</strong>g>r c<strong>on</strong>firm<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> correctness <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> findings.<br />

Reference <str<strong>on</strong>g>to</str<strong>on</strong>g> Fur<str<strong>on</strong>g>the</str<strong>on</strong>g>r Reading<br />

• Bulich, A. A. Use <str<strong>on</strong>g>of</str<strong>on</strong>g> Luminescent Bacteria for Determining Toxicity in Aquatic Envir<strong>on</strong>ments, in: L. L. Marking, R. A. Kimerle: Aquatic Toxicology, ASTM STP, American Society for<br />

Testing and Materials, vol. 667, pages 98-106, (1979)<br />

• Dannenberg, R.: <str<strong>on</strong>g>Water</str<strong>on</strong>g> - Waste <str<strong>on</strong>g>Water</str<strong>on</strong>g>, 135 no. 8, 475-480 (1994)<br />

• Kaiser, K. L., Palabrica, V. S., Pho<str<strong>on</strong>g>to</str<strong>on</strong>g>bacterium Phosphoreum Toxicity Data Index. <str<strong>on</strong>g>Water</str<strong>on</strong>g> Polluti<strong>on</strong> Research Journal <str<strong>on</strong>g>of</str<strong>on</strong>g> Canada, 26, 361-431 (1991)<br />

• Koller-Kreiml, V. and Rodinger, W., Aquatische Toxizität - ein wichtiges Kriterium zur Beurteilung v<strong>on</strong> Substanzen in Abwässern (Emissi<strong>on</strong>en) sowie zur Feststellung der <str<strong>on</strong>g>to</str<strong>on</strong>g>xischen<br />

Beeinträchtigung v<strong>on</strong> Oberflächengewässern (Immissi<strong>on</strong>en). Wasser und Abwasser 31 413-432 (1987)<br />

• Coleman R. M., Qureshi, A. A. Micro<str<strong>on</strong>g>to</str<strong>on</strong>g>x: Reserved and Spirillum Volutans Tests for Accessing Toxicity and Envir<strong>on</strong>mental Samples. B. Env. C<strong>on</strong>tam. and Tox. 35, 443-451 (1985)<br />

• Nohava, M. “Der Leuchtbakterientest in der Umweltk<strong>on</strong>trolle.” Untersuchungsbericht des Umweltbundesamtes UBA-1994-0909, February 1994, page 21.<br />

17


<strong>Grander®</strong> <strong>Technology</strong> Improves <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

The Preservati<strong>on</strong> Qualities<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> Drinking <str<strong>on</strong>g>Water</str<strong>on</strong>g><br />

My article “<str<strong>on</strong>g>Water</str<strong>on</strong>g> for Eternity”, which was published in <str<strong>on</strong>g>the</str<strong>on</strong>g> Grander Journal, vol. 1, 1999,<br />

(pages 42-47), provides additi<strong>on</strong>al informati<strong>on</strong> <strong>on</strong> this <str<strong>on</strong>g>to</str<strong>on</strong>g>pic. Here I present <str<strong>on</strong>g>the</str<strong>on</strong>g> most important<br />

findings <str<strong>on</strong>g>of</str<strong>on</strong>g> that report, al<strong>on</strong>g with <str<strong>on</strong>g>the</str<strong>on</strong>g> most recent test results.<br />

Pho<str<strong>on</strong>g>to</str<strong>on</strong>g>s: Dr. Felsch<br />

Chapter B17 <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> Austrian codex <str<strong>on</strong>g>of</str<strong>on</strong>g> food regulati<strong>on</strong>s<br />

pertains anytime drinking water is bottled and <str<strong>on</strong>g>of</str<strong>on</strong>g>fered<br />

for sale in Austria. It establishes that a bottle<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> water has <str<strong>on</strong>g>to</str<strong>on</strong>g> meet <str<strong>on</strong>g>the</str<strong>on</strong>g> following guideline figures 12 hours<br />

after it has been freshly filled:<br />

• max. 100 CFU/ml at 22° Celsius / 72 hours incubati<strong>on</strong> time and<br />

• max. 20 CFU/ml at 37° Celsius / 24 hours incubati<strong>on</strong> time.<br />

The most important requirement, however, is that in a<br />

250 ml water sample <str<strong>on</strong>g>the</str<strong>on</strong>g>re must be no microbes harmful <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

health (<str<strong>on</strong>g>the</str<strong>on</strong>g>se are prohibited by law).This specifically relates<br />

<str<strong>on</strong>g>to</str<strong>on</strong>g> Escherichia coli, coliform bacteria, enterococcus bacteria,<br />

Pseudom<strong>on</strong>as aeruginosa and Staphylococcus aureus. In additi<strong>on</strong>,<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> level <str<strong>on</strong>g>of</str<strong>on</strong>g> sulfite-reduced clostridia in 50 ml <str<strong>on</strong>g>of</str<strong>on</strong>g> water must<br />

be undetectable.<br />

These regulati<strong>on</strong>s establish requirements for <str<strong>on</strong>g>the</str<strong>on</strong>g> quality<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> bottled table water; <str<strong>on</strong>g>the</str<strong>on</strong>g>re are separate requirements<br />

for mineral water. Austrian law was adapted <str<strong>on</strong>g>to</str<strong>on</strong>g> European<br />

Uni<strong>on</strong> law in <str<strong>on</strong>g>the</str<strong>on</strong>g> Federal Law Gazette 309 <str<strong>on</strong>g>of</str<strong>on</strong>g> 1999, which was<br />

published <strong>on</strong> January 13, 2000. A c<strong>on</strong>sumer, <str<strong>on</strong>g>of</str<strong>on</strong>g> course, almost<br />

never has a bottle that was filled <strong>on</strong>ly 12 hours earlier. Usually<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> water has been <strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> shelf for some time before it arrives<br />

<strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> c<strong>on</strong>sumer’s table. What guidelines apply <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g>se<br />

water samples, which are usually somewhat older? Chapter<br />

B17 <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> codex has nothing <str<strong>on</strong>g>to</str<strong>on</strong>g> say about this. Publicati<strong>on</strong>s<br />

that address this field permit guideline figures <str<strong>on</strong>g>of</str<strong>on</strong>g> up <str<strong>on</strong>g>to</str<strong>on</strong>g> 1000<br />

CFU/ml if <str<strong>on</strong>g>the</str<strong>on</strong>g> microbes that are harmful <str<strong>on</strong>g>to</str<strong>on</strong>g> health, as menti<strong>on</strong>ed<br />

earlier, are absent. Here something clearly needs <str<strong>on</strong>g>to</str<strong>on</strong>g> be<br />

d<strong>on</strong>e <str<strong>on</strong>g>to</str<strong>on</strong>g> protect c<strong>on</strong>sumers.<br />

The Problem <str<strong>on</strong>g>of</str<strong>on</strong>g> Later Germinati<strong>on</strong><br />

As a reminder: for micro-organisms <str<strong>on</strong>g>to</str<strong>on</strong>g> survive in drinking<br />

water, <str<strong>on</strong>g>the</str<strong>on</strong>g> water must c<strong>on</strong>tain nutrients. This nutrient<br />

package is called AOC (assimilable organic carb<strong>on</strong>) and is<br />

expressed in µg carb<strong>on</strong> equivalent per litre. The lower limit<br />

needed for bacterial growth has been determined <str<strong>on</strong>g>to</str<strong>on</strong>g> be an<br />

AOC <str<strong>on</strong>g>of</str<strong>on</strong>g> 10 µg per litre; this is <str<strong>on</strong>g>the</str<strong>on</strong>g> AOC value below which<br />

bacteria cannot reproduce.<br />

In producing and bottling table water, <str<strong>on</strong>g>the</str<strong>on</strong>g> germinati<strong>on</strong><br />

problem would be solved if <strong>on</strong>ly water samples with an AOC<br />

c<strong>on</strong>tent <str<strong>on</strong>g>of</str<strong>on</strong>g> less than 10 µg per litre were used. However, such<br />

water samples are ra<str<strong>on</strong>g>the</str<strong>on</strong>g>r rare. Even very good drinking water<br />

usually has a higher AOC c<strong>on</strong>tent.<br />

What is d<strong>on</strong>e in practice <str<strong>on</strong>g>to</str<strong>on</strong>g> solve this problem? Carb<strong>on</strong>ic<br />

acid is added, which lowers <str<strong>on</strong>g>the</str<strong>on</strong>g> pH <str<strong>on</strong>g>to</str<strong>on</strong>g> less than 5, thus inhib-<br />

18


iting <str<strong>on</strong>g>the</str<strong>on</strong>g> reproducti<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> aquatic pseudom<strong>on</strong>ads. C<strong>on</strong>sumers<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g>ten find that <str<strong>on</strong>g>the</str<strong>on</strong>g> additi<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> this preservative gives water a<br />

particularly tingling and refreshing taste. The actual taste <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> water, however, is hardly discernible.<br />

<str<strong>on</strong>g>Water</str<strong>on</strong>g> Is Less Perishable with<br />

Grander ® <strong>Technology</strong><br />

Countless tests have shown that Grander ® <strong>Technology</strong>,<br />

without <str<strong>on</strong>g>the</str<strong>on</strong>g> use <str<strong>on</strong>g>of</str<strong>on</strong>g> additives or o<str<strong>on</strong>g>the</str<strong>on</strong>g>r measures, is capable <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

stabilising and permanently preserving drinking water even<br />

at room temperature. Grander ® revitalisati<strong>on</strong> leads <str<strong>on</strong>g>to</str<strong>on</strong>g> a<br />

breakdown <str<strong>on</strong>g>of</str<strong>on</strong>g> organic nutrient sources in <str<strong>on</strong>g>the</str<strong>on</strong>g> water. One <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> effects <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ® <strong>Technology</strong> is that <str<strong>on</strong>g>the</str<strong>on</strong>g> AOC c<strong>on</strong>tent<br />

in <str<strong>on</strong>g>the</str<strong>on</strong>g> water is quickly broken down, thus preventing <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

growth <str<strong>on</strong>g>of</str<strong>on</strong>g> microbes in <str<strong>on</strong>g>the</str<strong>on</strong>g> water.<br />

How Can <str<strong>on</strong>g>the</str<strong>on</strong>g> Nutrient<br />

Breakdown Be Explained?<br />

It would appear that <str<strong>on</strong>g>the</str<strong>on</strong>g> nutrients s<str<strong>on</strong>g>to</str<strong>on</strong>g>red in n<strong>on</strong>-revitalised<br />

drinking water are less attractive <str<strong>on</strong>g>to</str<strong>on</strong>g> bacteria. Even if <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

temperature <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> source water is raised from eight degrees<br />

Celsius <str<strong>on</strong>g>to</str<strong>on</strong>g> 25 degrees Celsius, <str<strong>on</strong>g>the</str<strong>on</strong>g> bacteria do not become<br />

substantially more active. A rise in <str<strong>on</strong>g>the</str<strong>on</strong>g> microbe count from<br />

5 CFU/ml <str<strong>on</strong>g>to</str<strong>on</strong>g> 140 CFU/ml within a m<strong>on</strong>th had almost no effect<br />

<strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> AOC c<strong>on</strong>tent.<br />

The Grander ® pin points, <strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> o<str<strong>on</strong>g>the</str<strong>on</strong>g>r hand, are substantially<br />

more active, and lower <str<strong>on</strong>g>the</str<strong>on</strong>g> AOC c<strong>on</strong>tent by 90%. Following<br />

proximity revitalisati<strong>on</strong> using Grander ® <strong>Technology</strong>,<br />

micro-organisms c<strong>on</strong>tained in <str<strong>on</strong>g>the</str<strong>on</strong>g> water begin <str<strong>on</strong>g>to</str<strong>on</strong>g> alter <str<strong>on</strong>g>the</str<strong>on</strong>g>ir<br />

behaviour. Suddenly <str<strong>on</strong>g>the</str<strong>on</strong>g> food supply in <str<strong>on</strong>g>the</str<strong>on</strong>g> water appears <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

become very usable by <str<strong>on</strong>g>the</str<strong>on</strong>g> pin points that have developed in<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> interim. The pin points react by multiplying sharply and<br />

c<strong>on</strong>suming all <str<strong>on</strong>g>the</str<strong>on</strong>g> available food. Thereafter, <str<strong>on</strong>g>the</str<strong>on</strong>g> pin points enter<br />

an inactive phase and assume <str<strong>on</strong>g>the</str<strong>on</strong>g> functi<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> a “surveillance<br />

Filled <strong>on</strong> March 13, 1994, this<br />

bottle is now six years old.<br />

Meanwhile, <str<strong>on</strong>g>the</str<strong>on</strong>g> date <str<strong>on</strong>g>of</str<strong>on</strong>g> manufacture<br />

is being printed <strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

bottles, with <str<strong>on</strong>g>the</str<strong>on</strong>g> expiry date<br />

given as two years later, but this<br />

could be extended <str<strong>on</strong>g>to</str<strong>on</strong>g> six years.<br />

The advice “s<str<strong>on</strong>g>to</str<strong>on</strong>g>re in a cool<br />

place” is no l<strong>on</strong>ger necessary.<br />

Breakdown <str<strong>on</strong>g>of</str<strong>on</strong>g> Organic Food Sources<br />

The following experiment serves <str<strong>on</strong>g>to</str<strong>on</strong>g> explain <str<strong>on</strong>g>the</str<strong>on</strong>g> phenomen<strong>on</strong><br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> rapid breakdown <str<strong>on</strong>g>of</str<strong>on</strong>g> AOC:<br />

Three samples were taken from a drinking-water<br />

source known <str<strong>on</strong>g>to</str<strong>on</strong>g> be <str<strong>on</strong>g>of</str<strong>on</strong>g> high quality:<br />

• The first sample (unrevitalised) was immediately sent in<br />

a cooled state <str<strong>on</strong>g>to</str<strong>on</strong>g> a health institute in order <str<strong>on</strong>g>to</str<strong>on</strong>g> determine<br />

its bacteriological quality.<br />

• The sec<strong>on</strong>d sample (unrevitalised) was sent <str<strong>on</strong>g>to</str<strong>on</strong>g> ano<str<strong>on</strong>g>the</str<strong>on</strong>g>r<br />

university institute, which was asked <str<strong>on</strong>g>to</str<strong>on</strong>g> determine its<br />

AOC c<strong>on</strong>tent.<br />

• The third sample was divided and placed in two sterilised<br />

glass bottles.<br />

• One glass bottle was s<str<strong>on</strong>g>to</str<strong>on</strong>g>red in such a way that it did<br />

not come in<str<strong>on</strong>g>to</str<strong>on</strong>g> c<strong>on</strong>tact with <strong>Grander®</strong> <strong>Technology</strong>.<br />

• The sec<strong>on</strong>d bottle was placed next <str<strong>on</strong>g>to</str<strong>on</strong>g> a bottle <str<strong>on</strong>g>of</str<strong>on</strong>g> Original<br />

<strong>Grander®</strong> <str<strong>on</strong>g>Water</str<strong>on</strong>g> and c<strong>on</strong>tact-revitalised.<br />

These two samples underwent bacteriological testing at<br />

<strong>on</strong>e-week intervals and <str<strong>on</strong>g>the</str<strong>on</strong>g> number <str<strong>on</strong>g>of</str<strong>on</strong>g> col<strong>on</strong>y forming<br />

units (CFU) was determined.<br />

After <strong>on</strong>e m<strong>on</strong>th all <str<strong>on</strong>g>the</str<strong>on</strong>g> test results were available:<br />

• First sample: <str<strong>on</strong>g>the</str<strong>on</strong>g> health institute found a <str<strong>on</strong>g>to</str<strong>on</strong>g>tal microbe<br />

level <str<strong>on</strong>g>of</str<strong>on</strong>g> 5 CFU/ml after 72 hours at 22 degrees Celsius. At<br />

37 degrees Celsius <str<strong>on</strong>g>the</str<strong>on</strong>g> level <str<strong>on</strong>g>of</str<strong>on</strong>g> CFU/ml was undetectable.<br />

The tests <str<strong>on</strong>g>to</str<strong>on</strong>g> determine <str<strong>on</strong>g>the</str<strong>on</strong>g> level <str<strong>on</strong>g>of</str<strong>on</strong>g> harmful microbes<br />

found n<strong>on</strong>e at all.<br />

• Sec<strong>on</strong>d sample: <str<strong>on</strong>g>the</str<strong>on</strong>g> AOC c<strong>on</strong>tent was found <str<strong>on</strong>g>to</str<strong>on</strong>g> be 125<br />

µg organic carb<strong>on</strong> per litre.<br />

• The unrevitalised sample s<str<strong>on</strong>g>to</str<strong>on</strong>g>red at room temperature<br />

for <strong>on</strong>e m<strong>on</strong>th showed a microbe increase from<br />

5 CFU/ml (starting-point) <str<strong>on</strong>g>to</str<strong>on</strong>g> 140 CFU/ml.<br />

• In <str<strong>on</strong>g>the</str<strong>on</strong>g> first week, massive pin point formati<strong>on</strong> was<br />

found in <str<strong>on</strong>g>the</str<strong>on</strong>g> c<strong>on</strong>tact-revitalised <strong>Grander®</strong> sample.<br />

In <str<strong>on</strong>g>the</str<strong>on</strong>g> sec<strong>on</strong>d week, 1,500 pin points per ml were<br />

counted. Starting in <str<strong>on</strong>g>the</str<strong>on</strong>g> third week, <str<strong>on</strong>g>the</str<strong>on</strong>g> number <str<strong>on</strong>g>the</str<strong>on</strong>g> pin<br />

points began <str<strong>on</strong>g>to</str<strong>on</strong>g> fall. After four weeks fewer than 10 pin<br />

points were detected.<br />

AOC determinati<strong>on</strong>s were c<strong>on</strong>ducted again <strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

revitalised and <str<strong>on</strong>g>the</str<strong>on</strong>g> unrevitalised samples (both four weeks<br />

old) producing <str<strong>on</strong>g>the</str<strong>on</strong>g> following results:<br />

• Drinking water sample, unrevitalised, s<str<strong>on</strong>g>to</str<strong>on</strong>g>red <strong>on</strong>e<br />

m<strong>on</strong>th at room temperature: AOC c<strong>on</strong>tent <str<strong>on</strong>g>of</str<strong>on</strong>g> 120 µg/l<br />

– representing a drop <str<strong>on</strong>g>of</str<strong>on</strong>g> 4%.<br />

• Drinking water, Grander ® -revitalised, s<str<strong>on</strong>g>to</str<strong>on</strong>g>red <strong>on</strong>e m<strong>on</strong>th<br />

at room temperature: AOC c<strong>on</strong>tent <str<strong>on</strong>g>of</str<strong>on</strong>g> 12 µg/l – representing<br />

a drop <str<strong>on</strong>g>of</str<strong>on</strong>g> 90%.<br />

19


The Course <str<strong>on</strong>g>of</str<strong>on</strong>g> Col<strong>on</strong>y Formati<strong>on</strong> in Revitalised and Unrevitalised <str<strong>on</strong>g>Water</str<strong>on</strong>g><br />

microbe count<br />

700<br />

600<br />

500<br />

400<br />

300<br />

200<br />

100<br />

0<br />

650<br />

390<br />

350<br />

200<br />

190<br />

210<br />

180<br />

160<br />

100<br />

25 14 10 5 0<br />

0<br />

Days 0 3 5 13 20 30<br />

mo<str<strong>on</strong>g>the</str<strong>on</strong>g>r col<strong>on</strong>ies pin points unrevitalised water<br />

Let us look first at <str<strong>on</strong>g>the</str<strong>on</strong>g> red curve:<br />

The drinking water used in <str<strong>on</strong>g>the</str<strong>on</strong>g> test had an initial value <str<strong>on</strong>g>of</str<strong>on</strong>g> 25 CFU/ml. After <str<strong>on</strong>g>the</str<strong>on</strong>g> water was<br />

allowed <str<strong>on</strong>g>to</str<strong>on</strong>g> stand for three days at room temperature, <str<strong>on</strong>g>the</str<strong>on</strong>g> <str<strong>on</strong>g>to</str<strong>on</strong>g>tal microbe count was 160.<br />

According <str<strong>on</strong>g>to</str<strong>on</strong>g> drinking-water regulati<strong>on</strong>s, this water would be <strong>on</strong>ly c<strong>on</strong>diti<strong>on</strong>ally potable. The<br />

microbe count rose in two additi<strong>on</strong>al days <str<strong>on</strong>g>to</str<strong>on</strong>g> 200 and after 30 days <str<strong>on</strong>g>of</str<strong>on</strong>g> s<str<strong>on</strong>g>to</str<strong>on</strong>g>rage had reached<br />

210 CFU per ml. Thus in 30 days <str<strong>on</strong>g>the</str<strong>on</strong>g> <str<strong>on</strong>g>to</str<strong>on</strong>g>tal microbe count in <str<strong>on</strong>g>the</str<strong>on</strong>g> unrevitalised drinking water<br />

rose by a fac<str<strong>on</strong>g>to</str<strong>on</strong>g>r <str<strong>on</strong>g>of</str<strong>on</strong>g> eight.<br />

Let us look now at <str<strong>on</strong>g>the</str<strong>on</strong>g> blue and green curves:<br />

Part <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> water menti<strong>on</strong>ed was subjected <str<strong>on</strong>g>to</str<strong>on</strong>g> revitalisati<strong>on</strong> in a parallel revitalisati<strong>on</strong> experiment,<br />

in which <str<strong>on</strong>g>the</str<strong>on</strong>g> so-called Grander ® Energy Rod was placed for about 10 sec<strong>on</strong>ds in a<br />

volume <str<strong>on</strong>g>of</str<strong>on</strong>g> 500 ml water and <str<strong>on</strong>g>the</str<strong>on</strong>g>n removed. (The rod, <str<strong>on</strong>g>of</str<strong>on</strong>g> course, had been sterilised.)<br />

Five days after this revitalisati<strong>on</strong>, <str<strong>on</strong>g>the</str<strong>on</strong>g> number <str<strong>on</strong>g>the</str<strong>on</strong>g> pin points had risen <str<strong>on</strong>g>to</str<strong>on</strong>g> 650. The “wild”<br />

col<strong>on</strong>ies had dropped <str<strong>on</strong>g>to</str<strong>on</strong>g> 10. After this maximum, both <str<strong>on</strong>g>the</str<strong>on</strong>g> pin points and <str<strong>on</strong>g>the</str<strong>on</strong>g> “wild”<br />

col<strong>on</strong>ies declined. After 20 days <str<strong>on</strong>g>the</str<strong>on</strong>g> number <str<strong>on</strong>g>of</str<strong>on</strong>g> “wild” col<strong>on</strong>ies had dropped <str<strong>on</strong>g>to</str<strong>on</strong>g> zero;<br />

after 30 days no pin points were detected.<br />

troop”. This troop immediately reactivates when nutrients<br />

appear. This is proved by <str<strong>on</strong>g>the</str<strong>on</strong>g> following experiment. A single<br />

drop <str<strong>on</strong>g>of</str<strong>on</strong>g> sterile bouill<strong>on</strong> soluti<strong>on</strong> is added <str<strong>on</strong>g>to</str<strong>on</strong>g> Grander ® -revitalised<br />

drinking water with an AOC c<strong>on</strong>tent <str<strong>on</strong>g>of</str<strong>on</strong>g> 12 µg/l. This soluti<strong>on</strong><br />

is s<str<strong>on</strong>g>to</str<strong>on</strong>g>red for two days at room temperature. A microbe<br />

count will <str<strong>on</strong>g>the</str<strong>on</strong>g>n reveal <str<strong>on</strong>g>the</str<strong>on</strong>g> following results: about 12,000 pin<br />

points have formed <strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> membrane filter after an incubati<strong>on</strong><br />

period <str<strong>on</strong>g>of</str<strong>on</strong>g> 72 hours at 22 degrees Celsius. Grander ® revitalisati<strong>on</strong><br />

and <str<strong>on</strong>g>the</str<strong>on</strong>g> high activity <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g>se pin points thus guarantee<br />

complete natural purity <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> water.<br />

Mode <str<strong>on</strong>g>of</str<strong>on</strong>g> Acti<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ®<br />

<strong>Technology</strong> in <str<strong>on</strong>g>the</str<strong>on</strong>g> Bacteriological<br />

Stabilisati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> Drinking <str<strong>on</strong>g>Water</str<strong>on</strong>g><br />

The previous experiment was based <strong>on</strong> a closed system:<br />

for example, drinking water that has been bottled so that<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g>re can be no fur<str<strong>on</strong>g>the</str<strong>on</strong>g>r additi<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> nutrients or pollutants.<br />

Revitalisati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> water samples in closed systems will result<br />

in <str<strong>on</strong>g>the</str<strong>on</strong>g> l<strong>on</strong>g-term stability, as described.<br />

But what is <str<strong>on</strong>g>the</str<strong>on</strong>g> situati<strong>on</strong> in <str<strong>on</strong>g>the</str<strong>on</strong>g> case <str<strong>on</strong>g>of</str<strong>on</strong>g> open systems, such<br />

as drinking-water pipes? Here fresh quantities <str<strong>on</strong>g>of</str<strong>on</strong>g> water are<br />

flowing through <str<strong>on</strong>g>the</str<strong>on</strong>g> revitalisati<strong>on</strong> equipment, c<strong>on</strong>stantly<br />

bringing new nutrient materials with <str<strong>on</strong>g>the</str<strong>on</strong>g>m.<br />

Experience has shown that over <str<strong>on</strong>g>the</str<strong>on</strong>g> course <str<strong>on</strong>g>of</str<strong>on</strong>g> about two<br />

m<strong>on</strong>ths after Grander ® <strong>Technology</strong> has been installed, an<br />

equilibrium is reached. The number <str<strong>on</strong>g>of</str<strong>on</strong>g> pin points settles<br />

down <str<strong>on</strong>g>to</str<strong>on</strong>g> a value that is dependent <strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> AOC c<strong>on</strong>tent <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

drinking water. As was already menti<strong>on</strong>ed in <str<strong>on</strong>g>the</str<strong>on</strong>g> previous<br />

chapter <strong>on</strong> pin point formati<strong>on</strong>, within about two m<strong>on</strong>ths<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> installati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> revitalisati<strong>on</strong> equipment in a drinkingwater<br />

pipe, <str<strong>on</strong>g>the</str<strong>on</strong>g> entire system <str<strong>on</strong>g>of</str<strong>on</strong>g> pipes will also have been<br />

revitalised. This is most likely a c<strong>on</strong>tributing fac<str<strong>on</strong>g>to</str<strong>on</strong>g>r <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

good revitalisati<strong>on</strong> results achieved in practice. In stagnant<br />

water <str<strong>on</strong>g>the</str<strong>on</strong>g> activity is <str<strong>on</strong>g>of</str<strong>on</strong>g>ten even more intense. More pin points<br />

are usually found <str<strong>on</strong>g>the</str<strong>on</strong>g>re than in samples <str<strong>on</strong>g>of</str<strong>on</strong>g> flowing drinking<br />

water.<br />

Original Grander ® <str<strong>on</strong>g>Water</str<strong>on</strong>g> -<br />

Found <str<strong>on</strong>g>to</str<strong>on</strong>g> Keep for Six Years<br />

The sample bottle <str<strong>on</strong>g>of</str<strong>on</strong>g> Original Grander ® <str<strong>on</strong>g>Water</str<strong>on</strong>g> c<strong>on</strong>cerned<br />

was bottled <strong>on</strong> March 13, 1994, and has been s<str<strong>on</strong>g>to</str<strong>on</strong>g>redat room<br />

temperature for use in l<strong>on</strong>g-term stability tests.<br />

Only four weeks after <str<strong>on</strong>g>the</str<strong>on</strong>g> bottle was filled – mid-April 1994<br />

– <str<strong>on</strong>g>the</str<strong>on</strong>g> <str<strong>on</strong>g>to</str<strong>on</strong>g>tal microbe count <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> water had fallen <str<strong>on</strong>g>to</str<strong>on</strong>g> an undetectable<br />

level, and <str<strong>on</strong>g>the</str<strong>on</strong>g> microbe count has not changed <str<strong>on</strong>g>to</str<strong>on</strong>g> this day.<br />

Microbes harmful <str<strong>on</strong>g>to</str<strong>on</strong>g> health have never been present. Thus even<br />

after six years, this Original Grander ® <str<strong>on</strong>g>Water</str<strong>on</strong>g> meets <str<strong>on</strong>g>the</str<strong>on</strong>g> standards<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> chapter B 17 <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> Austrian codex <str<strong>on</strong>g>of</str<strong>on</strong>g> health regulati<strong>on</strong>s. In<br />

additi<strong>on</strong>, samples have been taken since 1994 <str<strong>on</strong>g>of</str<strong>on</strong>g> every batch in<br />

order <str<strong>on</strong>g>to</str<strong>on</strong>g> test its state <str<strong>on</strong>g>of</str<strong>on</strong>g> preservati<strong>on</strong>. This individual bottle is not<br />

unique in its capacity <str<strong>on</strong>g>to</str<strong>on</strong>g> keep for many years; this property has<br />

been c<strong>on</strong>firmed by many o<str<strong>on</strong>g>the</str<strong>on</strong>g>r sample analyses.<br />

Summary<br />

This report is intended <str<strong>on</strong>g>to</str<strong>on</strong>g> dem<strong>on</strong>strate <str<strong>on</strong>g>the</str<strong>on</strong>g> significance<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ® <strong>Technology</strong> in <str<strong>on</strong>g>the</str<strong>on</strong>g> preservati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> drinking<br />

water. With <str<strong>on</strong>g>the</str<strong>on</strong>g> excepti<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ® <strong>Technology</strong>, I am<br />

aware <str<strong>on</strong>g>of</str<strong>on</strong>g> no process that is capable <str<strong>on</strong>g>of</str<strong>on</strong>g> preserving drinking<br />

water without <str<strong>on</strong>g>the</str<strong>on</strong>g> additi<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> chemicals or <str<strong>on</strong>g>the</str<strong>on</strong>g> implementati<strong>on</strong><br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> physical measures. The preservati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> drinking<br />

water is probably <str<strong>on</strong>g>the</str<strong>on</strong>g> most important task facing us in <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

new millennium.<br />

20


How L<strong>on</strong>g Is Original Grander ® Revitalisati<strong>on</strong> Equipment Effective?<br />

The Active Life-Span <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

Grander ® Revitalisers<br />

The product descripti<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ® water revitalisati<strong>on</strong> equipment states <str<strong>on</strong>g>the</str<strong>on</strong>g> following: Grander<br />

® revitalisati<strong>on</strong> equipment operates with natural energy, without electricity,<br />

without additives and is maintenance and service free. This raises <str<strong>on</strong>g>the</str<strong>on</strong>g> questi<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> how l<strong>on</strong>g<br />

Grander ® equipment, which has no replacement parts and is “<strong>on</strong>ly” filled with<br />

Grander ® Informati<strong>on</strong> <str<strong>on</strong>g>Water</str<strong>on</strong>g>, remains effective?<br />

To answer this questi<strong>on</strong>, an effectiveness test was performed<br />

<strong>on</strong> Grander ® revitalisati<strong>on</strong> equipment, some<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> which was more than 10 years old. Pin point formati<strong>on</strong><br />

was chosen as a method <str<strong>on</strong>g>of</str<strong>on</strong>g> establishing pro<str<strong>on</strong>g>of</str<strong>on</strong>g>.<br />

One method for determining <str<strong>on</strong>g>the</str<strong>on</strong>g> effectiveness <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander<br />

® <strong>Technology</strong> is by m<strong>on</strong>i<str<strong>on</strong>g>to</str<strong>on</strong>g>ring pin point formati<strong>on</strong>. The<br />

pin point method determines whe<str<strong>on</strong>g>the</str<strong>on</strong>g>r <str<strong>on</strong>g>the</str<strong>on</strong>g> water structure<br />

altered by Grander ® <strong>Technology</strong> is capable <str<strong>on</strong>g>of</str<strong>on</strong>g> effecting “wild”<br />

col<strong>on</strong>ies in such a way that <str<strong>on</strong>g>the</str<strong>on</strong>g>ir appearance changes <str<strong>on</strong>g>to</str<strong>on</strong>g> that<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> pin points. The advantages <str<strong>on</strong>g>of</str<strong>on</strong>g> this method are tw<str<strong>on</strong>g>of</str<strong>on</strong>g>old:<br />

first, <str<strong>on</strong>g>the</str<strong>on</strong>g> revitalisati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> living organisms – namely, bacteria<br />

– can be measured; sec<strong>on</strong>d, <str<strong>on</strong>g>the</str<strong>on</strong>g> pin point analysis can<br />

be c<strong>on</strong>ducted al<strong>on</strong>g <str<strong>on</strong>g>the</str<strong>on</strong>g> lines <str<strong>on</strong>g>of</str<strong>on</strong>g> standardised and generally<br />

recognised bacteriological methods.<br />

Pho<str<strong>on</strong>g>to</str<strong>on</strong>g>s: Dr. Felsch<br />

Up <str<strong>on</strong>g>to</str<strong>on</strong>g> now, <str<strong>on</strong>g>the</str<strong>on</strong>g>re has been no method capable <str<strong>on</strong>g>of</str<strong>on</strong>g> measuring<br />

informati<strong>on</strong> transmissi<strong>on</strong> directly. Thus it has not<br />

been possible <str<strong>on</strong>g>to</str<strong>on</strong>g> determine <str<strong>on</strong>g>the</str<strong>on</strong>g> effectiveness <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ®<br />

revitalisati<strong>on</strong> equipment using ei<str<strong>on</strong>g>the</str<strong>on</strong>g>r electric or electr<strong>on</strong>ic<br />

instruments.<br />

The Developmental His<str<strong>on</strong>g>to</str<strong>on</strong>g>ry <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

Grander ® Revitalisati<strong>on</strong> Equipment<br />

The first equipment <str<strong>on</strong>g>of</str<strong>on</strong>g> this kind (1989-1993) c<strong>on</strong>sisted <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

b<strong>on</strong>ded plastic pipes suitable for drinking water with external<br />

brass cartridges filled with Grander ® Informati<strong>on</strong> <str<strong>on</strong>g>Water</str<strong>on</strong>g>.<br />

Since 1994 Original Grander ® revitalisati<strong>on</strong> equipment<br />

has been manufactured in an integrated unit c<strong>on</strong>structi<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

stainless steel. There are no l<strong>on</strong>ger external brass cartridges<br />

but ra<str<strong>on</strong>g>the</str<strong>on</strong>g>r internal chambers filled with Original Grander ®<br />

Informati<strong>on</strong> <str<strong>on</strong>g>Water</str<strong>on</strong>g>.<br />

21


Col<strong>on</strong>y Forming<br />

Units in<br />

N<strong>on</strong>-revitalised <str<strong>on</strong>g>Water</str<strong>on</strong>g><br />

1 ml <str<strong>on</strong>g>of</str<strong>on</strong>g> undiluted liquid was<br />

drawn through a membrane<br />

filter impermeable <str<strong>on</strong>g>to</str<strong>on</strong>g> bacteria<br />

(pore size .45 µm, with counting<br />

grid). This was <str<strong>on</strong>g>the</str<strong>on</strong>g>n incubated<br />

<strong>on</strong> standard nutrient agar with<br />

added TTC at 22 degrees Celsius.<br />

N<strong>on</strong>-revitalised water from St Ulrich am Pillersee.<br />

Sample <str<strong>on</strong>g>of</str<strong>on</strong>g> March 22, 2000. 3 CFU/ml.<br />

After 48 hours <str<strong>on</strong>g>of</str<strong>on</strong>g> incubati<strong>on</strong>, <str<strong>on</strong>g>the</str<strong>on</strong>g> “wild” col<strong>on</strong>ies<br />

have reached <str<strong>on</strong>g>the</str<strong>on</strong>g>ir full size. The water has not been<br />

revitalised (no pin points).<br />

Descripti<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

Effectiveness Test<br />

All <str<strong>on</strong>g>the</str<strong>on</strong>g> equipment tested had been used in practical<br />

applicati<strong>on</strong>s; that means that until its testing it was installed<br />

in drinking-water pipes. After being dismantled, <str<strong>on</strong>g>the</str<strong>on</strong>g> equipment<br />

was brought <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> labora<str<strong>on</strong>g>to</str<strong>on</strong>g>ry.<br />

• The test began <strong>on</strong> March 22, 2000.<br />

• The dismantled Grander ® revitalisati<strong>on</strong> equipment was first<br />

rinsed with scalding hot, n<strong>on</strong>-revitalised water in order <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

cleanse it <str<strong>on</strong>g>of</str<strong>on</strong>g> any attached impurities and <str<strong>on</strong>g>to</str<strong>on</strong>g> remove any<br />

airborne microbes that might have entered <str<strong>on</strong>g>the</str<strong>on</strong>g> equipment<br />

pipes during dismantling or transport.<br />

• After <str<strong>on</strong>g>the</str<strong>on</strong>g> equipment had been cooled, n<strong>on</strong>-revitalised<br />

drinking water (from St Ulrich am Pillersee) was run through<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> equipment for 25 sec<strong>on</strong>ds at a rate <str<strong>on</strong>g>of</str<strong>on</strong>g> 1 l/s.<br />

• Afterwards, <str<strong>on</strong>g>the</str<strong>on</strong>g> equipment that had been filled with this<br />

water was allowed <str<strong>on</strong>g>to</str<strong>on</strong>g> stand for 15 minutes. This method was<br />

chosen because it reflects household practice and simulates<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> typical practice <str<strong>on</strong>g>of</str<strong>on</strong>g> water usage.<br />

• When <str<strong>on</strong>g>the</str<strong>on</strong>g> 15 minutes were over, <str<strong>on</strong>g>the</str<strong>on</strong>g> water that remained<br />

in each revitaliser was poured in<str<strong>on</strong>g>to</str<strong>on</strong>g> a sterilised 400-ml beaker<br />

where <str<strong>on</strong>g>the</str<strong>on</strong>g> volume was determined. The results were as<br />

follows:<br />

Revitalisers made in 1989, blue plastic pipe: 223 ml<br />

Revitalisers made in 1991, green plastic pipe: 225 ml<br />

Revitalisers made in 1997, stainless steel: 387 ml<br />

• These beakers were <str<strong>on</strong>g>the</str<strong>on</strong>g>n sealed and s<str<strong>on</strong>g>to</str<strong>on</strong>g>red in such a way<br />

that proximity revitalisati<strong>on</strong> by Grander ® equipment or<br />

Original Grander ® drinking water was impossible.<br />

• At <str<strong>on</strong>g>the</str<strong>on</strong>g> same time, in order <str<strong>on</strong>g>to</str<strong>on</strong>g> arrive at a base bacteriological<br />

value, <str<strong>on</strong>g>the</str<strong>on</strong>g> n<strong>on</strong>-revitalised water from St Ulrich was subjected<br />

<str<strong>on</strong>g>to</str<strong>on</strong>g> bacteriological examinati<strong>on</strong>. This n<strong>on</strong>-revitalised<br />

water was also poured in<str<strong>on</strong>g>to</str<strong>on</strong>g> a 400-ml beaker and allowed <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

stand.<br />

• The first bacteriological examinati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> individual beakers<br />

was made after 24 hours and <str<strong>on</strong>g>the</str<strong>on</strong>g> sec<strong>on</strong>d after 48 hours<br />

(<str<strong>on</strong>g>the</str<strong>on</strong>g>se are depicted above).<br />

Results <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

Effective Period Tests<br />

When <str<strong>on</strong>g>the</str<strong>on</strong>g> results <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> pin point tests are compared, <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

following c<strong>on</strong>clusi<strong>on</strong>s can be drawn:<br />

• In this test, all three revitalisers completely revitalised<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> drinking water from St Ulrich am Pillersee. After an<br />

incubati<strong>on</strong> period <str<strong>on</strong>g>of</str<strong>on</strong>g> 48 hours at 22 degrees Celsius no “wild”<br />

col<strong>on</strong>ies (large mo<str<strong>on</strong>g>the</str<strong>on</strong>g>r col<strong>on</strong>ies) were seen <strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g> filter.<br />

There were <strong>on</strong>ly pin points.<br />

22


• Comparing <str<strong>on</strong>g>the</str<strong>on</strong>g> revitalisati<strong>on</strong> equipment <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> first<br />

generati<strong>on</strong> made in 1989 (with blue plastic pipe) with <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

modern integrated unit equipment <str<strong>on</strong>g>of</str<strong>on</strong>g> stainless steel (made<br />

in 1997) no significant difference in <str<strong>on</strong>g>the</str<strong>on</strong>g> number <str<strong>on</strong>g>the</str<strong>on</strong>g> pin<br />

points could be determined. It should be pointed out that in<br />

bacteriological tests <str<strong>on</strong>g>the</str<strong>on</strong>g> results can vary by plus or minus<br />

15% <str<strong>on</strong>g>to</str<strong>on</strong>g> 20%.<br />

• The blind test results: <str<strong>on</strong>g>the</str<strong>on</strong>g> n<strong>on</strong>-revitalised drinking<br />

water from St Ulrich am Pillersee was filled in<str<strong>on</strong>g>to</str<strong>on</strong>g> a 400ml<br />

c<strong>on</strong>tainer and after 48 hours <str<strong>on</strong>g>the</str<strong>on</strong>g> bacteria count determined.<br />

Result: two “wild col<strong>on</strong>ies” and 13 pin points. This shows,<br />

that in a labora<str<strong>on</strong>g>to</str<strong>on</strong>g>ry, where <str<strong>on</strong>g>the</str<strong>on</strong>g>re is extensive work with<br />

revitalised water, it is impossible <str<strong>on</strong>g>to</str<strong>on</strong>g> rule out some minimal<br />

“proximity revitalisati<strong>on</strong>”.<br />

Effectiveness Test <str<strong>on</strong>g>of</str<strong>on</strong>g> Three<br />

Different <strong>Grander®</strong> Models<br />

The effectiveness <str<strong>on</strong>g>of</str<strong>on</strong>g> three older models <str<strong>on</strong>g>of</str<strong>on</strong>g> Grander ®<br />

water revitalisers was tested with regard <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> formati<strong>on</strong><br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> pin points.<br />

<strong>Grander®</strong> <str<strong>on</strong>g>Water</str<strong>on</strong>g> Revitaliser Made in 1989<br />

As <str<strong>on</strong>g>the</str<strong>on</strong>g> illustrati<strong>on</strong> shows, producti<strong>on</strong><br />

checks were made as early as 1989. The<br />

revitalisati<strong>on</strong> equipment was subjected <str<strong>on</strong>g>to</str<strong>on</strong>g><br />

a pressure <str<strong>on</strong>g>of</str<strong>on</strong>g> up <str<strong>on</strong>g>to</str<strong>on</strong>g> 12 bars and this was<br />

noted <strong>on</strong> a sticker with <str<strong>on</strong>g>the</str<strong>on</strong>g> date (Oc<str<strong>on</strong>g>to</str<strong>on</strong>g>ber<br />

25, 1989) and a signature. This permitted<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> age <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> equipment <str<strong>on</strong>g>to</str<strong>on</strong>g> be determined<br />

exactly <str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> day.<br />

After 48 hours<br />

incubati<strong>on</strong> at 22° C.<br />

No mo<str<strong>on</strong>g>the</str<strong>on</strong>g>r col<strong>on</strong>ies,<br />

<strong>on</strong>ly pin point<br />

<strong>Grander®</strong> <str<strong>on</strong>g>Water</str<strong>on</strong>g> Revitaliser Made in 1991<br />

Blind test: Bacterial result <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> n<strong>on</strong>-revitalised water<br />

Final Assessment<br />

The openings where <str<strong>on</strong>g>the</str<strong>on</strong>g> Informati<strong>on</strong><br />

<str<strong>on</strong>g>Water</str<strong>on</strong>g> was filled in<str<strong>on</strong>g>to</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g> cartridges were<br />

sealed, and <str<strong>on</strong>g>the</str<strong>on</strong>g>se seals have not been<br />

broken. The pressure-test sticker is missing<br />

from this equipment, but <strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g><br />

brass cartridge itself a note has been<br />

made with a felt-tip pen: “Installed <strong>on</strong><br />

April 8, 1991”.<br />

<str<strong>on</strong>g>Based</str<strong>on</strong>g> <strong>on</strong> <str<strong>on</strong>g>the</str<strong>on</strong>g>se results, <str<strong>on</strong>g>the</str<strong>on</strong>g> c<strong>on</strong>clusi<strong>on</strong> may be drawn that<br />

Grander ® revitalisati<strong>on</strong> equipment keeps its powers <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

revitalisati<strong>on</strong> forever.<br />

After 48 hours:<br />

<strong>on</strong>ly pin points<br />

Since 1993 I have c<strong>on</strong>ducted about 2,000 water tests where<br />

Grander ® revitalisati<strong>on</strong> equipment was installed. At<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g>se installati<strong>on</strong>s, some <str<strong>on</strong>g>of</str<strong>on</strong>g> which were <str<strong>on</strong>g>of</str<strong>on</strong>g> an industrial<br />

nature, <str<strong>on</strong>g>the</str<strong>on</strong>g>re were dem<strong>on</strong>strable improvements in water<br />

quality and reducti<strong>on</strong> in <str<strong>on</strong>g>the</str<strong>on</strong>g> use <str<strong>on</strong>g>of</str<strong>on</strong>g> chemicals and envir<strong>on</strong>mental<br />

damage. A positive pin point test always went<br />

hand in hand with a measurable alleviati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> existing<br />

water problems.<br />

In many <str<strong>on</strong>g>of</str<strong>on</strong>g> <str<strong>on</strong>g>the</str<strong>on</strong>g>se examinati<strong>on</strong>s, both internal company<br />

testing as well as external labora<str<strong>on</strong>g>to</str<strong>on</strong>g>ries were involved.<br />

The technical measurements c<strong>on</strong>firmed both <str<strong>on</strong>g>the</str<strong>on</strong>g> existence<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> pin point formati<strong>on</strong> as well as an improvement<br />

in water quality.<br />

<strong>Grander®</strong> <str<strong>on</strong>g>Water</str<strong>on</strong>g> Revitaliser Made in 1997<br />

Current equipment made <str<strong>on</strong>g>of</str<strong>on</strong>g> stainless<br />

steel has integrated chambers filled<br />

with Grander ® Informati<strong>on</strong> <str<strong>on</strong>g>Water</str<strong>on</strong>g>.<br />

In flowing through <str<strong>on</strong>g>the</str<strong>on</strong>g> equipment,<br />

<str<strong>on</strong>g>the</str<strong>on</strong>g> water <str<strong>on</strong>g>to</str<strong>on</strong>g> be revitalised comes in<str<strong>on</strong>g>to</str<strong>on</strong>g><br />

indirect c<strong>on</strong>tact with <str<strong>on</strong>g>the</str<strong>on</strong>g> Informati<strong>on</strong><br />

<str<strong>on</strong>g>Water</str<strong>on</strong>g> in <str<strong>on</strong>g>the</str<strong>on</strong>g> chambers.<br />

After 48 hours:<br />

countless pin points<br />

23


7/02<br />

24

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!