Annual report 2009/10 - Human Fertilisation and Embryology Authority

Section 7
Issues identified in the 2009/10
horizon scanning process
21
Gamete selection and manipulation (continued)
Use of aim Description Reference
Techniques to avoid
the transmission of
mitochondrial disease
Mitochondrial genome
replacement in eggs –
studies in non-primates
using a technique called
spindle- chromosomal
complex transfer.
Metaphase II karyotype
transfer.
Ooplasm donation – studies
using ICSI and its effect on
the function of the paternal
genome.
Tachibana M et al. (2009) Mitochondrial gene replacement
in primate offspring and embryonic stem cells.
Nature 461:367-372.
Tanaka A et al. (2009) Metaphase II karyoplast transfer from
human in-vitro mature oocytes to enucleated mature oocytes.
RBMO 19(4):514-520.
Liang CG et al. (2009) Effects of ooplasm transfer on paternal
genome function in mice. Hum Rep 24(11):2718-2728.
Egg selection
Factors determining the size
and quality of ovarian reserve.
Hartshorne GM et al. (2009) Oogenesis and cell death in
human prenatal ovaries: what are the criteria for oocyte
selection? Molecular Hum Rep 2009 15(12):805-819.
Sperm quality
The effect of sperm
incubation and freezing on
genome integrity.
Feliciano M et al. (2009) Environmental factors on sperm
genomic integrity. Hum Rep 24 suppl 1:i236-i256.
In vitro maturation
(IVM) of eggs
The effectiveness of and
advances in the technique
and application of IVM. For
example, the use of IVM
following retrieval of eggs
from patients about to
undergo chemotherapy and
use of artificial constructs to
aid maturation.
Albuz FK et al. (2009) Substantial improvements in embryos
yield using a novel system of induced IVM by exploiting
cAMP modulators in pre-IVM and IVM. Hum Rep 24
suppl 1:i236-i256.
Amorim C et al. (2009) Survival of human pre-antral follicle after
cryopreservation of ovarian tissue, follicular isolation and in vitro
culture in calcium alginate matrix. Hum Rep 24(1):92-99.
Cao Y et al. (2009) Cryopreservation of immature and in-vitro
matured human oocytes by vitrification. RBMO 19(3):369-373.
Chian RC et al. (2009) Obstetric outcomes following
vitrification of in vitro and in vivo matured oocytes. Fertility and
Sterility 91(6):2391-2398.
Chian RC et al. (2009) Live birth after vitrification of in vitro
matured human oocytes. Fertility and Sterility 91(2):372-376.
Epic J et al. (2009) Effect of in vitro maturation of mouse
oocytes on the health and lifespan of adult offspring.
Hum Rep 24(4):922-928.
Kim IW et al. (2009) Derivation of developmentally competent
oocytes by the culture of preantral follicles retrieved from adult
ovaries: maturation, blastocyst formation, and embryonic stem
cell transformation. Fertility and Sterility 92(5):1716-1724.
Human Fertilisation & Embryology Authority
Scientific Horizon Scanning at the HFEA