General Study Guide - Ontario Police College

Leucomalachite Green (RCMP)
extremely sensitive to the presence of blood and will yield positive
results with dilutions of blood as low as 1 part in 1 million.
Green
Leucomalachite green reacts with the protein in
blood to produce a green color.
Cyanoacrylate fuming – non-porous substrates
Prior to fuming, the moisture in the latent print residue may be regenerated
by exposure to acetic vapours, thus improving quality.
Research has shown that applying CA before using a general protein
stain achieved no improvement in the enhancement of bloody prints.
With glass and metal surfaces, CA is harmful for further processing with
Coomassie Blue, Crowle’s reagent, and amido black dye.
White
Fumes from the active cyanoacrylate ester
polymerizes components of the print residue.
Post-cyano dye stains &/or regular or magnetic
powders are used to further enhance the
developed prints. Refer to separate table for a list
of possible dye stains and appropriate forensic
light sources.
Titanium Dioxide (OPC Notes)
6 or 7 spray applications with continuous agitation of mixture in bottle.
Leave for 30 seconds.
Methanol rinse applied in same manner as working solution.
Allow to air dry.
White
For developing bloody prints on dark surfaces.
Acid Yellow (OPC Notes) Yellow View when dry using alternate light source at
450nm and orange goggles.
Luminol (OPC & RCMP Notes)
An alkaline solution of Luminol will oxidize in the presence of an
oxidizing agent such as hydrogen peroxide or sodium perborate and a
Hematin-catalyzed peroxidase system, such as that found in blood.
This oxidation
reaction produces a
blue
chemiluminescence.
1. Darken crime scene.
2. Set-up camera for photography.
3. Wearing face shield and officer protection
suits spray area with luminol solution.
4. Immediately photograph
chemiluminescence.
Repeat spraying step if chemiluminescence
fades. Note - False positive reactions are
common.