蕨类植物组织培养研究进展（综述）

2002,31( ):37-43.
, 571737
Q949.36; Q943.1
A
1009-7791(2002)S0-0037-07
A review of study in tissue culture of ferns
ZENG Xia, ZHUANG Nan-sheng
(College of Agronomy, South China University of Tropical Agriculture, Danzhou 571737, Hainan China)
The advances in tissue culture of ferns are reviewed in this paper. The
techniques of in vitro propagation are described, including spore germination and clonal
propagation from sporophytes. The effects of cultural medium, cultural method and
cultural condition on tissue culture of ferns are summarized. The inducement and
regulation of altering generations in tissue culture of ferns are also introduced.
ferns; spore germination; tissue culture; altering generation
12 000 [1]
[2]
[3]
[4,5]
[6-8]
[4,5]
(Cyathea australis)
2002-07-22
1976-
1

38
31
1
1.1
[4,5]
[4,5]
1.1.1
24
Davallia bullata Platycerium bifurcatum
[9-11]
12 [10]
GA 3
50mg/L GA 3 25min
1 2 [10]
MS [9,12-15]
MS
MS [10] Knudson [16,17] Knop [18]
Nephroleasis multiflora
[10,13]
1.1.2
[17,18]
Osmunda regalisPteris ensiformis
[19] pH
[20]
Douglas [21]
1.1.3
1mg/L ABA [5]
Knauss [14]
partial tissue culture method
2
1/2MS 120
31
[15,22-24]
Osmurda regalis
[19] 1.2
[25-27] [28-30] [31-33]
[34,35]
[36,37]
GGB
green globular body

,:
( ) 39
1.2.1
MS
Higuchi [25,33] (Nephroleasis)1/4MS
(Aslenium nidus) MS
Dykeman [37] 1/2MS Matteuacia struthiopteris
3/4MS
Loescher [31]
MS
MS
1/2MS MS
80%89%
60%82%Harper [38] P
MS + P
MFMM
[31,32] MS ,
N [30] 6 Pragre [26]
1.2.2
NAA KT
BA [31] Rumohra adiantiformis
BA
[26] Beck Nephrolepis falcate forma
furcans
KT 12mg/L + NAA 0.01mg/L
[32]
KT 12mg/L
NAA
[37] Harper [38]
KT NAAKT 12mg/LNAA 0.110mg/L
2,4-D
NAA
BA
(Pteris vittata)
[28]
[25,33,39] 1987
Murashige [40]
Higuchi [33]
GGB
BA
GGB
GGB
GGB
[25] [29,41] (Blechmun spicant) [42]
(Nephrolepsis
exaltata) [39] (Pteris ensiformis) [27]
GGB
2,4-D
2-ip
4-PUTD2GA 3 JA
[43-46] 1.2.3
Loescher [31]
20g/L
3045 g/L
75g/L
[37]
[39] 1.2.4
Loescher [31]
24 pH 5.46.0
pH 7.0 [47] GGB
GGB
[25,33]

40
31
GGB
1/2MS
[24,48] 2
[4,5]
2.1
[49-54]
[52,55]
[55]
Fe-EDTA N
[50]
[50] IAA [56]
NAABA2,4-DKT 0.11mg/L
[42,55,57] 2.2
25ft-c [50]
[57,58]
[58]
[57,59] 3
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