Dr. Kamal Krishna Biswas - Rajshahi University

Curriculum Vitae
Name
: Kamal Krishna Biswas
Father’s name :Tarapada Biswas
Nationality : Bangladesh
Date of Birth : 1 st January 1967
Place of Birth : Rajbari, Bangladesh
Marrital Status : Married
Religion
: Sonaton( Hindu)
Language proficiency : Excellent in Bengali and English, Good at Japanese
Fellowship / Degree obtained and Educational Institutions :
JSPS Post Doctoral Fellowship( Nov., 2006 to October, 2008)
Deptt. of Laboratory and Vascular Medicine, Faculty of Medical and Dental Sciences
Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-8520, Japan.
Tel : +81-99-275-5437(Lab.), Fax- +81-275-2629(Lab.)
Ph.D. (September 26, 2005)
Field : Molecular Cell Biology and Immunology
Deptt. of Laboratory and Molecular Medicine
Graduate School of Medical Sciences
Kagoshima University, Kagoshima-shi, Japan
M.Sc. in Biochemistry (1989 held in 1993*)
Deptt. of Biochemistry
University of Rajshahi, Rajshahi-6205, Bangladesh
First class, Position: First
B.Sc(Hons.) in Biochemistry (1988 held in 1991*)
Deptt. of Biochemistry
University of Rajshahi, Rajshahi-6205, Bangladesh
First class, Position: First
H.S.C. (Science Group)
Kushtia Govt. College, Kushtia
Year of passing 1985; First Division
S.S.C. (Science Group)
Kashbamajail A.H. High School, Pangsha, Rajbari
Year of Passing: 1983; First Division
Positions Held :
Associate Professor ( June 08, 2006 to date)
Deptt. of Biochemistry and Molecular Biology
University of Rajshahi, Rajshahi-6205, Bangladesh

Assistant professor (May 05, 1999 to June 07, 2006)
Deptt. of Biochemistry
University of Rajshahi, Rajshahi-6205, Bangladesh
Lecturer (1 st April, 1996 to May 04, 1999)
Deptt. of Biochemistry
University of Rajshahi, Rajshahi-6205, Bangladesh
Lecturer ( May 05, 1994 to March 31, 1996) in Biochemistry
Patuakhali Agricultural College, Dumki, Patuakhali, Bangladesh
Research Experience : Cell biology and Immunology: Programmed cell death (Apoptosis)
and Inflammatory cytokine/mediators release, Platelets aggregation, Extraction ,purification
and characterization of proteins from different biological sources etc.
Techniques learnt : Cell culture, cell proliferation and programmed cell death assays,
stable and transient transfection. electrophoresis, immunoprecipitation, Western blotting,
Northern blotting, Immunohistochemistry, FACS-Analysis, extraction of DNA and RNA ,
real-time RT-PCR, Cellular fractionation(extraction of cytoplasmic, mitochondrial and
nuclear proteins), Gel shift assay, ELISA , extraction, purification and characterization of
proteins from different biological sources etc.
Research experience :
i) Role of endocannabinoids, uric acid etc. on the release of HMGB1( High
mobility group box 1) protein, a recently identified pro-inflammatory cytokine
and tissue damaged signal molecule, by monocytes/macrophages, endothelial
cells, vascular smooth muscle cells etc. as well as mechanisms of suppression of
inflammatory cytokine(LPS, TNF-a etc.)-induced HMGB1 and other
inflammatory mediators ( iNOS, COX-2, matrix metalloproteinases etc.) release
from monocytes/macrophages and endothelial cells and in vivo (mice models)
using different agents.
ii) Apoptosis and autophagy of glioma cells and apoptosis of neuronal cells, chang
liver cells, hepatoblastoma (HepG2 , HuH 7), primary hepatocytes, induced by
different stimulants .
iii) Tissue factor expression and Platelets aggregation.
Present research works: Screening of bioactive compounds from medicinally important
plants using different solvents and investigating their potentiality in antimicrobial and antiinflammatory
actions as well as their mechanism of actions.
List of publications :
a.Publications in Peer Reviewed Journal:

1* Kamal Krishna Biswas, Salunya Tancharoen, Krishna Pada Sarker, Ko-ichi Kawahara,
Teruto Hashiguchi and Ikuro Maruyama Cepharanthine triggers apoptosis in a human
hepatocellular carcinoma cell line (HuH-7) through the activation of JNK1/2 and the
down regulation of Akt FEBS Lett. 580(2): 703-710, January 2006.
2.*Kamal Krishna Biswas, Krishna Pada Sarker, Kazuhiro Abeyama, Ko-ichi Kawahara,
Satoshi Iino, Yasuharu Otsubo, Kazuhiko Saigo, Hiroyuki Izumi, Teruto Hashiguchi,
Munekazu Yamakuchi, Kazuyo Yamaji, Ryujin Endo, Kazuyuki Suzuki, Hitoshi
Imaizumi H, and Ikuro Maruyama. Membrane cholesterol but not putative receptors
mediates anandamide-induced hepatocyte apoptosis. Hepatology, 38(5):1167-77, Nov.
2003.
3. Oyama Y, Hashiguchi T, Taniguchi N, Tancharoen S, Uchimura T, Biswas KK,
Kawahara K, Nitanda T, Umekita Y, Lotz M, Maruyama I. High-mobility group box-1
protein promotes granulomatous nephritis in adenine-induced nephropathy. Lab Invest.
2010 Jun;90(6):853-66. Epub 2010 Mar 15.
4. Kozono S, Matsuyama T, Biswas KK, Kawahara K, Nakajima Y, Yoshimoto T,
Yonamine Y, Kadomatsu H, Tancharoen S, Hashiguchi T, Noguchi K, Maruyama I.
Involvement of the endocannabinoid system in periodontal healing. Biochem Biophys
Res Commun. 2010 Apr 16;394(4):928-33. Epub 2010 Mar 15.
5. Kikuchi K, Tancharoen S, Matsuda F, Biswas KK, Ito T, Morimoto Y, Oyama Y,
Takenouchi K, Miura N, Arimura N, Nawa Y, Meng X, Shrestha B, Arimura S, Iwata M,
Mera K, Sameshima H, Ohno Y, Maenosono R, Tajima Y, Uchikado H, Kuramoto T,
Nakayama K, Shigemori M, Yoshida Y, Hashiguchi T, Maruyama I, Kawahara K.
Edaravone attenuates cerebral ischemic injury by suppressing aquaporin-4.
Biochem Biophys Res Commun. 2009 Dec 25;390(4):1121-5. Epub 2009 Sep 6.
6. Kikuchi K, Kawahara KI, Biswas KK, Ito T, Tancharoen S, Morimoto Y, Matsuda F,
Oyama Y, Takenouchi K, Miura N, Arimura N, Nawa Y, Jie MX, Shrestha B, Arimura S,
Iwata M, Mera K, Sameshima H, Ohno Y, Maenosono R, Yoshida Y, Tajima Y,
Uchikado H, Kuramoto T, Nakayama K, Shigemori M, Hashiguchi T, Maruyama I.
Minocycline attenuates both OGD-induced HMGB1 release and HMGB1-induced cell
death in ischemic neuronal injury in PC12 cells. Biochem Biophys Res Commun. 2009
[Epub ahead of print]
7. Kikuchi K, Kawahara K, Tancharoen S, Matsuda F, Morimoto Y, Ito T, Biswas KK,
Takenouchi K, Miura N, Oyama Y, Nawa Y, Arimura N, Iwata M, Tajima Y, Kuramoto
T, Nakayama K, Shigemori M, Yoshida Y, Hashiguchi T, Maruyama I. The free radical
scavenger edaravone rescues rats from cerebral infarction by attenuating the release of
high-mobility group box-1 in neuronal cells. J Pharmacol Exp Ther. 2009
Jun;329(3):865-74.
8. Arimura N, Ki-i Y, Hashiguchi T, Kawahara K, Biswas KK, Nakamura M, Sonoda Y,
Yamakiri K, Okubo A, Sakamoto T, Maruyama I. Intraocular expression and release of
high-mobility group box 1 protein in retinal detachment. Lab Invest. 2009
Mar;89(3):278-89.
9. Mashahiro Iwata, Ko-ichi Kawahara, Hisashi Kawabata, Takashi Ito, Kamal Krishna

Biswas, Yuko Higashi, Kiyoshi kikuchi, Teruto Hashiguchi, Takuro Kanekura and Ikuro
Maruyama. Cytoprotective effect of thrombomodulin in UVB-irradiated epidermal
keratinocytes via the p38MAPK-CREB signaling pathway. Biochem Biophys Res
Commun. 2008 Dec 12; 377(2):642-7.
10. Kawahara K, Hashiguchi T, Kikuchi K, Tancharoen S, Miura N, Ito T, Oyama Y, Nawa
Y, Biswas KK, Meng X, Morimoto Y, Shrestha B, Sameshima H, Maruyama I. Induction
of high mobility group box 1 release from serotonin-stimulated human umbilical vein
endothelial cells. Int J Mol Med. 2008 Nov;22(5):639-44.
11.* Ko-ichi Kawahara, Kamal Krishna Biswas, Masako Unoshima, Takashi Ito, Kiyoshi
Kikuchi, Yoko Morimoto, Toshiaki Shimizu, Yoko Oyama, Kazunori Takenouchi, Shinichiro
Arimura, Nobuo Taniguchi, Toshihiko Nakajima, Hideo Iwasaka, Teruto
Hashiguchi and Ikuro Maruyama. CRP induces HMGB1 protein release through
activation of p38 MAPK in macrophage cell line, RAW264.7 cells. Cardiovascular
Pathology 2008 May-Jun;17(3):129-38.
12. Ko-ichi Kawahara, Kentaro Setoyama, Kiyoshi Kikuchi, Kamal Krishna Biswas,
Ryozo Kamimura, Masahiro Iwata, Takashi Ito, Yoko Morimoto, Teruto Hashiguchi,
Sonshin Takao, and Ikuro Maruyama.HMGB1 release in co-cultures of porcine
endothelial and human T cells. Xenotransplantaion 2007 Nov; 14(6):636-41.
13.*Katsumi Inoue, Ko-ichi Kawahara, Kamal Krishna Biswas, Kenji Ando, Kazuaki
Mitsudo, Masakiyo Nobuyoshi, Ikuro Maruyama. HMGB1 expression by activated
vascular smooth muscle cells in advanced human atherosclerosis plaques.
Cardovascular Pathol. 2007, 16(3), 136-43.
14. Arimura S, Kawahara K, Kamal Krishna Biswas , Abeyama K, Tabata M, Shimoda T,
Ogomi D, Matsusaki M, Kato S, Ito T, Sugihara K, Akashi M, Hashiguchi T, Maruyama
I. Hydroxyapatite formed on/in agarose gel induces activation of blood coagulation and
platelets aggregation. J Biomed Mater Res B Appl Biomater. 2007 May;81(2):456-61
15. Yumiko Nakajima, Yasushi Furuichi, Kamal Krishna Biswas,Teruto Hashiguchi, Koichi
Kawahara, Kazuo Yamaji, Tomonori Uchimura, Yuichi Izumi and Ikuro Maruyama..
Endocannabinoid, anandamide in gingival tissue regulates the periodontal inflammation
through NF-kB pathway inhibition. FEBS Lett. 580(2): 613-619, January 2006.
16. *Krishna Pada Sarker, Kamal Krishna Biswas Kazuyo Yamaji, Munekazu Yamakuchi,
Teruto Hashiguchi 1 , Ki-Young Lee and Ikuro Maruyama . Inhibition of Thrombin-
Induced Vascular Endothelial Growth Factor Production in Human Neuroblastoma
(NB-1) Cells by Argatroban. Pathophysiology of Haemostasis and
Thrombosis .34(1): 41-47, 2005.
17. Salunya Tancharon, Krishna Pada Sarker,Takahisa Imamura, Kamal Krishna Biswas
Kenji Matsushita, Shoko Tatsuyama, James Travis, Jan Potempa, Mitsuo Torii and
Ikuro Maruyama Neuropeptide release from dental pulp cells by RgpB via PAR-2
signalling. J Immunol. 174(9):5796-804. May, 2005.
18. Kazuyo Yamaji, Yin Wang, Yan Liu, Kazuhiro Abeyama, Teruto Hashiguchi
Tomonory Uchimora, Kamal Krishna Biswas, Hisahiko Iwamoto, Ikuro Maruyama.
Activated protein C, a natural anticoagulant protein,has antioxidant properties and
inhibits lipid peroxidation and advanced glycation end products formation. Thromb
Res; 115 (4):319-25 . 2005.
19. *Krishna Pada Sarker, Kamal Krishna Biswas, Jesusa L. Rosales, Kazuyo Yamaji,
Teruto Hashiguchi, Ki-Young Lee, Ikuro Maruyama. Ebselen inhibits NO-induced
apoptosis of differentiated PC12 cells via inhibition of ASK1-p38 MAPK-p53 and JNK
signaling and activation of p44/42 MAPK and Bcl-2. J Neurochem 87(6):1345-53 ,
Dec.2003.
20. *Krishna Pada Sarker, Kamal Krishna Biswas, Munekazu Yamakuchi, Ki-Young Lee,

Teruto Hashiguchi, Michael Kracht, Isao Kitajima, Ikuro Maruyama. ASK1-p38
MAPK/JNK signaling cascade mediates anandamide-induced PC12 cell death. J
Neurochem. 85(1):50-61, Apr. 2003.
21. Ranajit Kumar Shaha, Niranjan Kumar Sana , Narayn Roy, Kamal Krishna Biswas,
and Abdullah Mamun. “Partial Purification And Characterization of Protease from
germinating wheat seeds”. Pakistan J. of Biol Science 5(3), 317-320 March 2002.
22. Ranajit Kumar Shaha, Kamal Krishna Biswas and Niranjan Kumar Sana.:
Improvement in the Functional Properties of Gluten by Protease from germinating
wheat seeds. Bangladesh J. Biochem. 7: 16-26, 2001.
23. Parvin, Sahana., Niranjan Kumar Sana. Kamal Krishna Biswas, Anil Chandra Deb,
B.Sikdar and Ranajit Kr. Shaha Degradation of seed storage substances of bean seeds
( Lablab purureus ) duringgermination. Bangladesh J. Genet. Biotechnol. 2(1&2):
147-150, June & December 2001.
24. Niranjan Kumar Sana, Narayan Roy, Kamal Krishna Biswas and Entazul M.Huque.
Isolation and characterization of carbohydrate binding protein(lectin) from sesame oil
seed (Sesamum indicum, Till). Pakistan J of Biol. Sciences, 4(2): 233-236, February
2001.
25. Nurul Absar, Kamal Krishna Biswas and Md.Rezaul Karim. Purification and
characterization of rape seed (Brassica campestris Linn.) glycoproteins. Rajshahi
University Studies, J of Science, Vol. 23-24, 53-62 1995-1996.
d. Abstract accepted / Symposium Proceedings (presentation in professional
conferences):
1. Kamal Krishna Biswas, Ko-ichi Kawahara, Teruto Hashiguchi and Ikuro Maruyama
N-Arachidonoyl dopamine triggers autophagy and apoptosis in malignant glioma cells.
HMRI Conference on Translational Cancer Research, 10 to 12 September 2008
Newcastle, NSW, Australia.
2.Kamal Krishna Biswas, Ko-ichi Kawahara, Kiyoshi Kikuchi, Yoko Oyama, Kazunori
Takenouchi, Toshiaki Shimizu, Teruto Hashiguchi and Ikuro Maruyama Curcumin
abrogates LPS-induced HMGB1 release in murine macrophage RAW264.7 cells. The 3 rd
International DAMPs and Allarmins Symposium, Aug 30 – Sept. 2, 2008, Pittsburgh,
Pensylvania, US
2. Kamal Krishna Biswas, KP Sarker, K Kawahara,K Abeyama, T. Hashiguchi, S.
Yamada and I. Maruyama. Endocannabinoid anandamide induces high mobility group
box-1(HMGB1) protein in monocytes and macrophages. The XXth congress of the
International Society on Thrombosis and Haemostasis, .6-12 Aug, 2005, Sydney,
Australia. Program Book, Page No. 371
3. Kamal Krishna Biswas, Y. Oyama, K.Abeyama, T. Hashiguchi, I. Maruyama. Uric
acid induces high mobility group box-1(HMGB1) protein in monocytes and
macrophages through p38MAPK, ERK1/2, JNK and AP-1 activation. 46 th American
Society of Hematology Conference, Dec 4-7, 2004, San Diego, California, USA.
Abstract in Blood Vol. 104 No 11, Page 200, Nov. 2004.